Detection of Candida in Cervical Cancer Tissue by Periodic Acid Shiff and Gomori Methenamine Silver Staining DOI Creative Commons

Ahmad Ridho’i,

Rebekah Juniati Setiabudi, Arthur Pohan Kawilarang

et al.

Journal of chemical health risks, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Introduction and Aim: Cervical cancer is a global health burden. Of all the incidences reported in Indonesia, cervical occupies second position as highest contributor to incidence throughout year, third leading cause of death. Tissue culture staining methods were used diagnose cancer. This study aimed compare Candida detection possibility vulvovaginal candidiasis risk factors tissue blocks stained with periodic acid-Schiff (PAS) Gomori methenamine silver (GMS). Methods: was an analytical observational using cross-sectional approach. compared PAS GMS detect fungi paraffin block from individuals diagnosed at Anatomical Pathology Laboratory Kediri Regency Regional General Hospital 2021–2022. The sampling Total carried out March December 2022. Data analyses descriptively performed. Results: dominated by patients aged 51–60 years who belonged decade 6. In this study, 32 tissues deparaffinized analyzed. A histopathological diagnosis examined biopsy samples obtained. total 81.25% had squamous cell carcinoma, 18.75% adenocarcinoma. findings show that can more than staining. Microscopic examination showed obvious contrast, marked black structures green area. Thirteen (40.7%) for vulvovaginitis. However, did not invasion cervix factor Conclusion: be tissue. has higher accuracy detecting carcinoma fungal infections occurrence require further investigation.

Language: Английский

Design of Protease-Responsive Antifungal Liposomal Formulation Decorated with a Lipid-Modified Chitin-Binding Domain DOI Open Access
Hendra Saputra, Muhammad Safaat, Pugoh Santoso

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3567 - 3567

Published: March 22, 2024

Candida albicans is a prevalent fungal pathogen that displays antibiotic resistance. The polyene antifungal amphotericin B (AmB) has been the gold standard because of its broad spectra, and liposomal formulation, AmBisome, used widely clinically in treating infections. Herein, we explored enhancing activity AmBisome by integrating small chitin-binding domain (LysM) chitinase A derived from Pteris ryukyuensis. LysM conjugated with lipid (LysM-lipid) was initially prepared through microbial transglutaminase (MTG)-mediated peptide tag-specific conjugation lipid-peptide substrate. formulation modified LysM-lipid conjugates had size distribution comparable to native liposomes but an increased zeta potential, indicating were anchored AmBisome. LysM-lipid-modified exhibited long-term stability at 4 °C while retaining capacity bind chitin. Nevertheless, efficacy against C. modest. We then redesigned new conjugate introducing linker containing thrombin digestion (TD) site C-terminus (LysM-TD linker-lipid), thereby facilitating liberation upon addition thrombin. This LysM-TD linker-lipid superior performance suppressing growth presence compared formulation. These results provide platform design stimuli-responsive formulations respond external environments thus advance treatment pathogenic fungi

Language: Английский

Citations

1
Understanding the Role of Candida Abicans in the Development of Cancer Cells, its Progression Mechanism, Possible Preventive Measures and Treatment DOI Open Access

Adejoke Mary Ajao

International Journal of Innovative Science and Research Technology (IJISRT), Journal Year: 2024, Volume and Issue: unknown, P. 2796 - 2808

Published: May 17, 2024

As a common human commensal fungus, Candida albicans may play an active part in cancer progression rather than just being present by chance people who have cancer. A lot of research has shown that having your body can make you more likely to get oral, esophageal, intestinal, and maybe even skin cancers. But needs be done find out exactly how this opportunistic pathogen adds the development The purpose review is summarize current data possible ways linked fungus help tumors grow using its cytochrome system DNA-damaging benzopyrene molecules. In addition, it change immune causing inflammation while blocking antitumor reactions. dimorphic which means between yeast forms with only one cell hyphal invade host cells them spread. protected biofilms, native microbiota makeup Infected tissues could also oncogenic signaling turned on virulence factors. Assuming link direct one, useful way tell are most Taking aim at fresh treatments better. well-planned prospective clinical studies still needed sure results associations animal models correct. Getting clear molecular processes will us understand new treat focus interact.

Language: Английский

Citations

1
Beyond bacteria: Role of non-bacterial gut microbiota species in inflammatory bowel disease and colorectal cancer progression DOI

Hania Haque,

Syeda Warisha Zehra,

Mohammad Shahzaib

et al.

World Journal of Gastroenterology, Journal Year: 2024, Volume and Issue: 30(36), P. 4078 - 4082

Published: Sept. 20, 2024

This letter emphasizes the need to expand discussions on gut microbiome’s role in inflammatory bowel disease (IBD) and colorectal cancer (CRC) by including often-overlooked non-bacterial components of human flora. It highlights how viral, fungal archaeal inhabitants respond towards dys-biosis contribute progression. Viruses such as bacteriophages target certain bacterial species modulate immune system. Other viruses found associated include Epstein-Barr virus, papillomavirus, John Cunningham cytomegalovirus, herpes simplex virus type 6. Fungi Candida albicans Malassezia forming tissue-invasive filaments producing cytokines, respectively. Archaea, mainly methanogens are also altering microbial fermentation pathways. correspondence, thus underscores significance considering pathological physiological mechanisms entire spectrum microbiota develop effective therapeutic interventions for both IBD CRC.

Language: Английский

Citations

1
Alcohol consumption modulates Candida albicans-induced oral carcinogenesis and progression DOI

Isabel O’Grady,

Jeff O’Sullivan

Journal of Oral Biosciences, Journal Year: 2023, Volume and Issue: 65(4), P. 293 - 304

Published: Oct. 6, 2023

Language: Английский

Citations

3
Enhancing immunity against Candida albicans infections through TIGIT knockout DOI Creative Commons
Ahmed Rishiq,

Mingdong Liu,

Ofer Mandelboim

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: Aug. 7, 2024

ABSTRACT T cell immunoreceptor with immunoglobulin and tyrosine-based inhibitory motif (ITIM) domain (TIGIT) is an receptor expressed by natural killer cells. Here, we used TIGIT knockout (KO) mice to demonstrate that mouse directly interacts Candida albicans . Reduced fungal growth colonization were observed when TIGIT-KO splenocytes co-cultured C. compared the wild type (WT). In a systemic candidiasis model, exhibited improved survival reduced body weight loss WT mice. Organ-specific burden assessment revealed significantly lower loads in kidneys, spleen, lungs of Finally, show agglutinin-like sequence proteins ALS6, ALS7, ALS9 are ligands for absence these abolishes effect vivo Our results identify significance modulating host defense against highlight potential therapeutic implications infections. IMPORTANCE ITIM

Language: Английский

Citations

0
Bee Pollen as a Source of Biopharmaceuticals for Neurodegeneration and Cancer Research: A Scoping Review and Translational Prospects DOI Creative Commons

Rachid Kacemi,

María G. Campos

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5893 - 5893

Published: Dec. 13, 2024

Bee Pollen (BP) has many advantageous properties relying on its multitargeting potential, a new tendency in managing challenging illnesses. In cancer and neurodegeneration, the multiple effects of BP could be unequaled importance need further investigation. Although still limited, available data interestingly spotlights some floral sources with promising activities line this Adopting scoping review methodology, we have identified crucial bioactivities that are widely recognized to individual compounds but remain completely untapped valuable bee cocktail. A wide range these been recently found endowed great potential modulating pivotal processes neurodegeneration pathophysiology. addition, ubiquitous only isolated, while number studied BPs remains extremely limited compared endless pool plant species worldwide. We also elucidated clinical profits from perspectives impeded by hurdles such as bioavailability phytocompounds, diversity lack phytochemical standardization BP, difficulty selective targeting pathophysiological mechanisms. finally present interesting insights guide future research pave way for urgently needed simplified investigations.

Language: Английский

Citations

0
Priming the Nutrition-Gut-Immuno-Oncology Axis DOI
Abhijoy Saha, Neelkamal Kapoor, Navkiran Kaur

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Detection of Candida in Cervical Cancer Tissue by Periodic Acid Shiff and Gomori Methenamine Silver Staining DOI Creative Commons

Ahmad Ridho’i,

Rebekah Juniati Setiabudi, Arthur Pohan Kawilarang

et al.

Journal of chemical health risks, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Introduction and Aim: Cervical cancer is a global health burden. Of all the incidences reported in Indonesia, cervical occupies second position as highest contributor to incidence throughout year, third leading cause of death. Tissue culture staining methods were used diagnose cancer. This study aimed compare Candida detection possibility vulvovaginal candidiasis risk factors tissue blocks stained with periodic acid-Schiff (PAS) Gomori methenamine silver (GMS). Methods: was an analytical observational using cross-sectional approach. compared PAS GMS detect fungi paraffin block from individuals diagnosed at Anatomical Pathology Laboratory Kediri Regency Regional General Hospital 2021–2022. The sampling Total carried out March December 2022. Data analyses descriptively performed. Results: dominated by patients aged 51–60 years who belonged decade 6. In this study, 32 tissues deparaffinized analyzed. A histopathological diagnosis examined biopsy samples obtained. total 81.25% had squamous cell carcinoma, 18.75% adenocarcinoma. findings show that can more than staining. Microscopic examination showed obvious contrast, marked black structures green area. Thirteen (40.7%) for vulvovaginitis. However, did not invasion cervix factor Conclusion: be tissue. has higher accuracy detecting carcinoma fungal infections occurrence require further investigation.

Language: Английский

Citations

1