Transgenesis in microalga <i>Chlamydomonas reinhardtii</i>: current approaches

Ecological genetics, Journal Year: 2024, Volume and Issue: 22(1), P. 47 - 62

Published: March 20, 2024

Microalgae are a rich source of biologically active substances natural origin, which have potential for use in pharmaceutical, agricultural, food and industrial production. Genetic engineering microalgae opens up great prospects creating improved strains that produce various additives, commercial enzymes, as well proteins therapeutic purposes — antibodies, hormones vaccines. Chlamydomonas reinhardtii P.A. Dang. is unicellular green alga, reference organism studying the genetics photosynthesis developing new genetic approaches microalgae. The advantages C. include ability to transform all three its genomes (nuclear, mitochondrial chloroplast), low cost ease cultivation, safety humans presence system post-translational modification proteins, makes this platform biotechnology. Over past few years, significant advances been made transgenesis reinhardtii, including techniques based on CRISPR/Cas9 genome editing technology. In review, we summarize available information current alga reinhardtii: 1) general principles transgenic constructs design transformation nuclear chloroplast genome, 2) popular selection markers used, 3) methods cell transformation, 4) using system.

Language: Английский

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(12), P. 630 - 630

Published: Dec. 7, 2023

Rheumatoid arthritis (RA) is an invalidating chronic autoimmune disorder characterized by joint inflammation and progressive bone damage. Dietary intervention important component in the treatment of RA to mitigate oxidative stress, a major pathogenic driver disease. Alongside traditional sources antioxidants, microalgae-a diverse group photosynthetic prokaryotes eukaryotes-are emerging as anti-inflammatory immunomodulatory food supplements. Several species accumulate therapeutic metabolites-mainly lipids pigments-which interfere pro-inflammatory pathways involved other inflammatory conditions. The advancement clinical uses microalgae requires continuous exploration phytoplankton biodiversity chemodiversity, followed domestication wild strains into reliable producers said metabolites. In addition, tractability microalgal genomes offers unprecedented possibilities establish microbes light-driven biofactories heterologous immunotherapeutics. Here, we review evidence-based mechanisms metabolites provide detailed coverage genetic engineering strategies enhance yields endogenous compounds develop innovative bioproducts.

Language: Английский

Small cells with big photosynthetic productivities: biotechnological potential of the Picochlorum genus

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский