Phage-encoded depolymerases as a strategy for combating multidrug-resistant Acinetobacter baumannii

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 24, 2024

, a predominant nosocomial pathogen, represents grave threat to public health due its multiple antimicrobial resistance. Managing patients afflicted with severe infections caused by drug-resistant

Language: Английский

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Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications

Infectious Diseases and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Antimicrobial resistance is a significant global public health issue, and the dissemination of antibiotic in Gram-positive bacterial pathogens has significantly increased morbidity, mortality rates, healthcare costs. Among them, Staphylococcus, especially methicillin-resistant Staphylococcus aureus (MRSA), causes wide range diseases due to its diverse pathogenic factors infection strategies. These bacteria also present issues veterinary medicine food safety. Effectively managing staphylococci-related problems necessitates concerted effort implement preventive measures, rapidly detect pathogen, develop new safe antimicrobial therapies. In recent years, there been growing interest using endolysins combat infections. enzymes, which are referred as lysins, unique class hydrolytic enzymes synthesized by double-stranded DNA bacteriophages. They possess glycosidase, lytic transglycosylase, amidase, endopeptidase activities, effectively destroying peptidoglycan layer resulting lysis. This property makes powerful agents, particularly against organisms with more accessible layers. Therefore, considering potential benefits compared conventional antibiotics, we have endeavored gather review characteristics uses derived from staphylococcal bacteriophages, well their antibacterial effectiveness spp. based on conducted experiments trials.

Language: Английский

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Novel antimicrobial strategies for diabetic foot infections: addressing challenges and resistance

Acta Diabetologica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Language: Английский

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Isolation and Characterization of a Broad-Spectrum bacteriophage Against Multi-drug Resistant Escherichia coli from waterfowl field

Poultry Science, Journal Year: 2025, Volume and Issue: 104(2), P. 104787 - 104787

Published: Jan. 10, 2025

Escherichia coli (E. coli) is a significant pathogen responsible for intestinal infections and foodborne diseases. The rise of antibiotic resistance poses challenge to global public health. Traditional therapy becoming increasingly ineffective, highlighting the urgent need innovative control strategies. This study explores potential bacteriophages as sustainable alternative traditional antibiotics. From 2021 2022, total 183 non-repetitive duck source fecal samples were collected from Mianyang City, Sichuan Province, 126 strains E. isolated. minimum inhibitory concentration (MIC) test showed that these exhibited high piperacillin (96.8%), tetracycline (88.9%), chloramphenicol (86.5%). It concerning 93.7% isolates are classified multidrug-resistant (MDR), posing threat existing treatment options. 20 isolated soil samples, among which 5 selected further analysis. Bacteriophage YP6 excellent lytic effects on MDR strains, especially strain MY104, well representative serotypes O1 MY51) O18 MY106). identification member Myoviridae family was conducted using transmission electron microscopy, it found have an optimal infection factor 0.1. Bacteriophages exhibit thermal pH stability, maintaining survival at temperatures up 60 °C ranges 4 10. Whole genome sequencing confirmed has double stranded DNA 139,323 base pairs (bp), no or virulence genes found, indicating low possibility horizontal gene transfer. In addition, effectively inhibits formation biofilm, key in chronic infections. vivo experiments Galleria mellonella (G. mellonella) larvae shown protective effect against infection. summary, bacteriophage expected become therapeutic agent due its broad host range, environmental biofilm inhibition properties. Future research should optimize preparations, evaluate safety efficacy animal models, establish clinical application plans field food safety.

Language: Английский

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Phage treatment of multidrug-resistant bacterial infections in humans, animals, and plants: The current status and future prospects

Infectious Medicine, Journal Year: 2025, Volume and Issue: 4(1), P. 100168 - 100168

Published: Feb. 6, 2025

Phages, including the viruses that lyse bacterial pathogens, offer unique therapeutic advantages, their capacity to antibiotic-resistant bacteria and disrupt biofilms without harming host microbiota. The lack of new effective antibiotics growing limitations existing have refocused attention on phage therapy as an option in complex clinical cases such burn wounds, cystic fibrosis, pneumonia. This review describes preclinical studies which has been both human veterinary medicine, agricultural context. In addition, critical challenges, narrow range bacteriophages, possibility resistance, regulatory constraints widespread use therapy, are addressed. Future directions include optimizing through strategies ranging from cocktails broadening genetic modification, using phages vaccines or biocontrol agents. future, if can be efficiently delivered, maintained a stable state, phage-antibiotic synergy achieved, will much needed treatment options. However, successful implementation within current standards practice also require considerable development infrastructure greater public acceptance. closing, this highlights promise backup substitute for antibiotics. It proposes role significant adjunct to, even replacement for, treating multidrug-resistant infections.

Language: Английский

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Recent insights on phage therapy against multidrug-resistant Acinetobacter baumannii

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 12, 2025

Abstract Acinetobacter baumannii is a prevalent clinical pathogen commonly found to be multidrug-resistant (MDR), causing serious life-threatening infections, particularly hospital-acquired infections with limited therapeutic options. The MDR phenotype developed against this critical increasingly globally, reaching pan-drug-resistant conferring non-susceptibility all antimicrobials used in its treatment according the standard guidelines. Therefore, it develop innovative approaches, such as phage therapy, considering rise drug-resistant A. infections. In review, we highlight and discuss up-to-date antimicrobial resistance of , use phages, their limitations, future perspectives treating addition, combination phages antimicrobials, preclinical studies including pharmacokinetics pharmacodynamics properties have been discussed.

Language: Английский

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Fighting Antibiotic Resistance: Insights Into Human Barriers and New Opportunities

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

The public health issue of bacterial multi-resistance to antibiotics has gained awareness among the public, researchers, and pharmaceutical sector. Nevertheless, spread antimicrobial resistance been considerably aggravated by human activities, climate change, subsequent increased release antibiotics, drug-resistant bacteria, antibiotic genes in environment. extensive use for medical veterinary purposes not only induced increasing but also other problems, including negative effects on patient's microbiome. Preventive strategies, new treatment modalities, surveillance are progressively set up. A comprehensive approach is, however, lacking urgently tackling this adverse situation. To address challenge, we discussed here main causes driving pollution environment factors favorable emergence drug resistance. We next propose some key priorities research, prevention, surveillance, education supervise an effective clinical sustainable response.

Language: Английский

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The Global Challenge of Campylobacter: Antimicrobial Resistance and Emerging Intervention Strategies

Tropical Medicine and Infectious Disease, Journal Year: 2025, Volume and Issue: 10(1), P. 25 - 25

Published: Jan. 16, 2025

Antimicrobial resistance (AMR) in Campylobacter species, particularly C. jejuni and coli, poses a significant public health threat. These bacteria, which are commonly found livestock, poultry, companion animals, wildlife, the leading causes of foodborne illnesses, often transmitted through contaminated poultry. Extensive exposure to antibiotics human veterinary medicine creates selection pressure, driving mechanisms such as point mutations, horizontal gene transfer, efflux pumps. Resistance fluoroquinolones, macrolides, tetracyclines complicates treatment increases risk severe infections. Drug-resistant is humans via food, water, direct contact with highlighting its zoonotic potential. Addressing this challenge requires effective interventions. Pre-harvest strategies like biosecurity immune-based methods reduce bacterial loads on farms, while post-harvest measures, including carcass decontamination freezing, limit contamination. Emerging approaches, bacteriocins natural antimicrobials, offer chemical-free alternatives. Integrated, multidisciplinary interventions across food chain essential mitigate AMR transmission enhance safety. Sustainable agricultural practices, antimicrobial stewardship, innovative solutions critical curbing protecting global health. Our review examines dynamics presents current Campylobacter-related AMR, offering valuable insights for control poultry industry.

Language: Английский

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The Gut Virome and Human Health: From Diversity to Personalized Medicine

Engineering Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 100191 - 100191

Published: Feb. 1, 2025

Language: Английский

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Isolation and Optimization of Phages Infecting Members of the Streptococcus bovis/Streptococcus equinus Complex

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 28 - 28

Published: March 4, 2025

Background: Cattle production is a cornerstone of U.S. agriculture but faces increasing pressure to balance profitability with environmental sustainability. Optimizing the ruminal microbiome enhance feed efficiency could help address both challenges. Members Streptococcus bovis/Streptococcus equinus complex (SBSEC) are key contributors acidosis and related digestive disorders due their role in carbohydrate fermentation lactic acid production. Bacteriophages targeting this bacterial group present promising approach mitigate problem high precision without promoting spread antibiotic resistance. Methods: A collection SBSEC-targeting bacteriophages were isolated from cattle rumen fluid feces further characterized. Characterization included host-range evaluation, whole genome sequencing, growth inhibition assessment via optical density measurements. Selected underwent training infectivity. Results: Eleven lytic one lysogenic phage isolated. Several phages demonstrated sustained suppression, showing efficacy against SBSEC bacteria diverse sources despite narrow host ranges. Co-evolutionary was done subset improve bacteriolytic activity had an inconsistent effect on ability inhibit naïve host. Genomic sequencing phylogenetic analysis revealed uniqueness clustering into three distinct groups that matched phenotypic characteristics. Conclusions: This study demonstrates potential as precise biological control agents, successful isolation enhancement bacteria. genome-sequenced show promise for development additives, though research needed optimize application agricultural settings.

Language: Английский

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