Published: March 28, 2025
Language: Английский
Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1920 - 1920
Published: Sept. 21, 2024
Antimicrobial resistance (AMR), frequently considered a major global public health threat, requires comprehensive understanding of its emergence, mechanisms, advances, and implications. AMR’s epidemiological landscape is characterized by widespread prevalence constantly evolving patterns, with multidrug-resistant organisms (MDROs) creating new challenges every day. The most common mechanisms underlying AMR (i.e., genetic mutations, horizontal gene transfer, selective pressure) contribute to the emergence dissemination resistant strains. Therefore, mitigation strategies (e.g., antibiotic stewardship programs—ASPs—and infection prevention control strategies—IPCs) emphasize importance responsible antimicrobial use surveillance. A One Health approach interconnectedness human, animal, environmental health) highlights necessity for interdisciplinary collaboration holistic in combating AMR. Advancements novel therapeutics alternative agents vaccines) offer promising avenues addressing challenges. Policy interventions at international national levels also promote ASPs aiming regulate use. Despite all observed progress, remains pressing concern, demanding sustained efforts address emerging threats sustainability. Future research must prioritize innovative approaches complex socioecological dynamics This manuscript resource researchers, policymakers, healthcare professionals seeking navigate develop effective mitigation.
Language: Английский
Citations
22
Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14
Published: Jan. 6, 2025
The rise of antibiotic-resistant pathogens has intensified the search for novel antimicrobial agents. This study aimed to isolate Streptomyces kanamyceticus from local soil samples and evaluate its properties, along with optimizing production bioactive compounds. Soil were collected regions, processed, analysed strains isolation using morphological characteristics molecular identification through 16S rRNA gene PCR assay. Antimicrobial activity was assessed against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Candida albicans double-layer method, while Minimum Inhibitory Concentration (MIC) values determined. extracted compounds underwent Fourier Transform Infrared Spectroscopy (FTIR) analysis functional group identification. Optimization compound performed a Central Composite Design (CCD) coupled Partial Least Squares Regression (PLSR). A total 25 distinct isolated, seven confirmed as S. kanamyceticus. These exhibited activity, inhibition zones reaching 30 mm MIC between 20 70 µg/mL. extraction yielded 150-200 mL studies revealed that medium containing 10 g/L glucose glycine max meal maximized antibiotic production. is promising source antibiotics. combination microbial isolation, testing, statistical optimization successfully enhanced compounds, contributing effective agents resistant pathogens.
Language: Английский
Citations
2
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 18, 2025
Language: Английский
Citations
2
Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 194, P. 106843 - 106843
Published: Aug. 6, 2024
Language: Английский
Citations
9
Published: July 11, 2024
The increasing emergence of multidrug resistant (MDR) pathogens, due to antibiotics misuse, translates in obstinate infections, with high morbidity and high-cost hospitalizations. To oppose these MDR superbugs, new antimicrobial options are necessary. Although both quaternary ammonium (QASs) phosphonium salts (QPSs) possess effects, QPSs have been studied at lesser extent. Recently, we successfully reported on the bacteriostatic cytotoxic effects a triphenyl salt against isolates Enterococcus Staphylococcus genus. Here, aiming finding antibacterial devices possibly bactericidal towards broader spectrum clinically relevant bacteria, responsible for severe human synthesized water-soluble, sterically hindered (BPPB). It encompasses two groups linked by C12 alkyl chain, thus embodying characteristics molecules known as bola-amphiphiles. BPPB was characterized ATR-FTIR, NMR UV spectroscopy, FIA-MS (ESI), elemental analysis potentiometric titrations. Optical DLS analyses evidenced tendency self-forming spherical vesicles solution (45 nm DLS), having positive zeta potential (+18 mV). were first time assessed fifty clinical Gram-positive Gram-negative species. Excellent observed all strains tested, involving most worrying species included ESKAPE bacteria. lowest MICs 0.250 µg/mL, while highest ones (32 µg/mL) metallo-β-lactamase-producing bacteria and/or also colistin, carbapenems, cefiderocol, so that intractable current available antibiotics. Moreover, when administered HepG2 hepatic Cos-7 monkey kidney cell lines, showed selectivity indices > 10 isolated superbugs such those E. coli species, being very promising be developed.
Language: Английский
Citations
8
Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14
Published: Jan. 27, 2025
Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a critical pathogen in healthcare settings, associated with high mortality due to its extensive antibiotic resistance. In this study, we report an outbreak of CRKP neonatal intensive care unit (NICU) within 200-bed tertiary hospital. The main goal study was characterize the phenotypic and genomic profiles isolates involved gain insights into their resistance mechanisms transmission dynamics NICU. Methods conducted between November December 2023 5-bed Monthly surveillance cultures were performed monitor colonization infection multidrug-resistant organisms. obtained from blood nasal swabs affected neonates. Identification antimicrobial susceptibility testing initially using Vitek ® 2 system N-395 card further confirmed by 16S rRNA sequencing. Whole-genome sequencing (WGS) (AMR) profiling identify genes virulence factors. For genetic analysis, both Illumina short-read Nanopore long-read used, followed hybrid assembly for enhanced genome resolution. Plasmid gene determined AMRFinder PlasmidFinder databases. Results A total three (designated Kp1, Kp2, Kp3) identified. Kp1 Kp2 belonged sequence type (ST) ST23 genetically near-identical, differing single allele, while Kp3 distinct type, ST2096, 245 allelic differences Kp2. All resistant colistin carried genes, including mcr-1 bla NDM-1, KPC2 confirming carbapenem Efflux pump aminoglycoside also detected, providing multifaceted defence against antibiotics. analysis identified several incompatibility groups (IncFI, IncHI, IncFIB, IncX), indicating potential horizontal transfer determinants. Conclusion This highlights complexity outbreaks care, exhibiting that complicate treatment. plasmid suggest these strains are reservoirs emphasizing need strict control ongoing surveillance. challenges increase risk treatment failures mortality, underscoring importance prevention novel therapeutic strategies.
Language: Английский
Citations
1
Life, Journal Year: 2025, Volume and Issue: 15(2), P. 242 - 242
Published: Feb. 5, 2025
Antimicrobial peptides (AMPs) are a diverse group of small, naturally occurring molecules that orchestrate the innate immune response various organisms, from microorganisms to humans. Characterized by their broad-spectrum activity against bacteria, fungi and viruses, AMPs increasingly recognized for potential as novel therapeutic agents in face rising antibiotic resistance. Here, we present several newly designed AMPs, one which, DTN6, exerts significant organisms with MIC values low 0.5 µg/mL. The D-TN6 peptide influences both bacteria yeasts. Scanning electron microscopy transmission results showed bacterial membrane is affected D-TN6, which resistant proteases effective antibiotic-resistant pathogens hemolytic toxicity. vivo standard S. aureus strains wounds. Thus, potent candidate broad spectrum activity. Overall, promising tool development next-generation antimicrobial could mitigate global health threats posed multidrug-resistant pathogens.
Language: Английский
Citations
1
Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 465 - 465
Published: May 31, 2024
Aeromonas dhakensis is increasingly recognised to be an important pathogen responsible for disease losses in warm-water aquaculture and, similar several other species, it can infect humans. Knowledge of A. accumulating, but this species remains relatively under-investigated compared its close relative, hydrophila. The significance may have been overlooked events aquatic animals due issues with reliable identification. Critical appreciating the importance application dependable molecular tools that enable accurate identification and discrimination from hydrophila motile aeromonads. This review aims synthesise key literature on dhakensis, particularly relevance aquaculture, including knowledge bacterium derived case studies hosts. Identification methods strain phylogeny are discussed, detection prompt diagnosis distinguishing strains heightened virulence. Increasing evidence suggests more virulent than correct required determine zoonotic risks posed, which includes concerns antibiotic-resistant strains. provides impetus improve future screen collections presumptive spp. retrospectively reveal true prevalence impact environment, healthcare settings.
Language: Английский
Citations
5
The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 961, P. 178400 - 178400
Published: Jan. 1, 2025
Urban water environments, including canals, harbours and estuaries are susceptible to contamination with antimicrobials drug-resistant bacteria through domestic industrial wastewater discharges storm overflows. There is potential for wildlife using these waters acquire transmit antimicrobial resistance genes (ARGs) of clinical importance. This study aimed assess clinically important in urban waterfowl, particularly mute swans. Faecal samples were collected from 17 swans the Greater Dublin Area, Ireland during July, August, October, November 2022. Samples swabbed directly onto agars select carbapenem resistant, Extended-spectrum Beta-lactamase (ESBL)-producing, ciprofloxacin resistant colistin bacteria. Isolates identified by MALDI-TOF as Enterobacterales tested susceptibility a panel 16 real-time PCR was employed detect cefotaximase carbapenemase (CRGs). Drug-resistant isolates characterised Whole Genome Sequencing (WGS), long read sequencing mobile (mcr) gene-producing isolates. Eleven seventeen (65 %) swan positive organism(s) (n = 35), comprising Escherichia coli 32; 82 other 3). Twenty E. (63 produced ESBL, 16/20 (80 bla
Language: Английский
Citations
0
Exploration of Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 10, 2025
Ventilator-associated pneumonia (VAP) is a common and serious complication in critically ill patients, particularly those requiring prolonged mechanical ventilation. Extensively drug-resistant (XDR) Acinetobacter baumannii has emerged as significant threat VAP cases, complicating treatment due to its exceptionally high level of antibiotic resistance. We present case 62-year-old male patient with severe acute ischemic stroke, who developed during his stay the intensive care unit (ICU). Cultures confirmed XDR causative organism. The infection was further complicated by development lung abscess, rare but consequence VAP. treated targeted antimicrobial regimen consisting colistin, vancomycin, tigecycline. Despite pathogen’s resistance profile, patient’s showed gradual improvement sustained treatment. However, recovery process significantly complexity infection, necessitating extended supportive hospitalization. This highlights challenges posed infections patients importance tailored approach effectively manage complications such abscess.
Language: Английский
Citations
0