Juglans regia and Pfaffia paniculata extracts: implications for periodontal disease treatment and correlation with Alzheimer’s risk DOI Creative Commons
Diego Garcia Miranda, Florence Carrouel, Nina Attik

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: May 16, 2025

Periodontal disease (PD) is a significant global health concern, affecting approximately 19% of the world’s population. It one most prevalent diseases today, causing substantial socio-economic impacts and diminished quality life. Recent research has also revealed potential link between PD Alzheimer’s disease. This study investigated antimicrobial effects Juglans regia Pfaffia paniculata extracts against P. endodontalis gingivalis , bacteria that cause are related to risk. The assessed impact these on macrophage metabolic activity, pro- anti-inflammatory cytokine expression, genotoxicity. phytochemical analysis extract was carried out first. Antimicrobial activity performed using M11-A7 protocol (CLSI) for planktonic cultures monotypic biofilms matured 168 hours in anaerobiosis. Cell viability MTT mouse macrophages (RAW 264-7), as well genotoxicity assessment micronuclei. evaluated ELISA method, checking cytokines IL-6, IL-1B, TNF-alpha, IL-17 IL-10. Phytochemical presence Miquelianin, Regiolone Gallic Acid J. extract. For extract, we identified glycosides Pfaffoside C, A, 3-O-β-D-glycopyranosyl-oleanolic acid Beta-ecdysone. MBC 1.73 0.48 . All were reduced by more than 89% after treatment with 5 min. Cytotoxicity evaluations cell remained above 50% at concentrations up 0.216 mg/ml 0.015 Neither exhibited Furthermore, both demonstrated promoting production In conclusion, activities suggest their treatments oral dysbiosis, which may contribute risk neurodegenerative diseases.

Language: Английский

Effects of Sucrose and Farnesol on Biofilm Formation by Streptococcus mutans and Candida albicans DOI Creative Commons

Wit Yee Wint,

Mayu Miyanohara,

Chika Terada-Ito

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1737 - 1737

Published: Aug. 22, 2024

Candida albicans (C. albicans) and Streptococcus mutans (S. mutans) are frequently detected in the plaque biofilms of children with early childhood caries. This study investigated effects sucrose farnesol on biofilm formation by oral pathogens S. C. albicans, including their synergistic interactions. Biofilm dynamics were monitored using Cell Index (CI). The CI for increased brain–heart infusion medium, peaking at 10 h; however, addition reduced CI. For yeast cells, concentrations > 0.5%, 2 h. Mixed cultures cells showed significantly higher values presence sucrose, suggesting a effect formation. Farnesol consistently suppressed even resulted greater inhibition. Regarding hyphal did not enhance formation, whereas all tested. These findings elucidate complex roles emphasize potential as an effective inhibitor.

Language: Английский

Citations

4
Juglans regia and Pfaffia paniculata extracts: implications for periodontal disease treatment and correlation with Alzheimer’s risk DOI Creative Commons
Diego Garcia Miranda, Florence Carrouel, Nina Attik

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: May 16, 2025

Periodontal disease (PD) is a significant global health concern, affecting approximately 19% of the world’s population. It one most prevalent diseases today, causing substantial socio-economic impacts and diminished quality life. Recent research has also revealed potential link between PD Alzheimer’s disease. This study investigated antimicrobial effects Juglans regia Pfaffia paniculata extracts against P. endodontalis gingivalis , bacteria that cause are related to risk. The assessed impact these on macrophage metabolic activity, pro- anti-inflammatory cytokine expression, genotoxicity. phytochemical analysis extract was carried out first. Antimicrobial activity performed using M11-A7 protocol (CLSI) for planktonic cultures monotypic biofilms matured 168 hours in anaerobiosis. Cell viability MTT mouse macrophages (RAW 264-7), as well genotoxicity assessment micronuclei. evaluated ELISA method, checking cytokines IL-6, IL-1B, TNF-alpha, IL-17 IL-10. Phytochemical presence Miquelianin, Regiolone Gallic Acid J. extract. For extract, we identified glycosides Pfaffoside C, A, 3-O-β-D-glycopyranosyl-oleanolic acid Beta-ecdysone. MBC 1.73 0.48 . All were reduced by more than 89% after treatment with 5 min. Cytotoxicity evaluations cell remained above 50% at concentrations up 0.216 mg/ml 0.015 Neither exhibited Furthermore, both demonstrated promoting production In conclusion, activities suggest their treatments oral dysbiosis, which may contribute risk neurodegenerative diseases.

Language: Английский

Citations

0