Trans, Trans-Farnesol Enhances the Anti-Bacterial and Anti-Biofilm Effect of Arachidonic Acid on the Cariogenic Bacteria Streptococcus mutans and Streptococcus sobrinus DOI Open Access

Farah Haj-Yahya,

Doron Steinberg,

Ronit Vogt Sionov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11770 - 11770

Published: Nov. 1, 2024

Background: Streptococcus mutans and sobrinus are Gram-positive bacteria involved in the development of dental caries, as they able to form biofilms on tooth enamel, ferment sugars into acids, survive under acidic conditions. This ultimately leads a local lowering pH value surface, which causes enamel cavities. Hypothesis: One measure reduce caries is limit growth cariogenic by using two anti-bacterial agents with different mechanisms action. The hypothesis this study was that activity ω-6 polyunsaturated arachidonic acid (AA) against S. can be enhanced sesquiterpene alcohol trans, trans-farnesol (t,t-farnesol). Methods: single combined treatment determined checkerboard assay. Bacterial viability assessed live/dead SYTO 9/propidium iodide (PI) staining flow cytometry. Anti-biofilm MTT metabolic assay, crystal violet biofilm biomass, 9/PI spinning disk confocal microscopy (SDCM) high-resolution scanning electron (HR-SEM). Results: t,t-Farnesol lowered minimum inhibitory concentration (MIC) (MBIC) AA at sub-MICs. reduced preformed mature biofilms, while t,t-farnesol had no significant effect. effect t,t-farnesol/AA further evidenced increased PI uptake, indicating membrane perforation. anti-biofilm verified SDCM HR-SEM. Gene expression studies showed some biofilm-related genes. Conclusions: Altogether, our suggests potential use naturally occurring compounds for preventing formation sobrinus. These findings have implications prevention.

Language: Английский

Preventing Oral Dual Biofilm Development with Innovative Bioactive Varnishes DOI Creative Commons

Tainá de Lima Costa,

Regina Maria Puppin‐Rontani, Aline Rogéria Freire de Castilho

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(2), P. 70 - 70

Published: Feb. 18, 2025

This study introduces innovative varnishes incorporating natural bioactive compounds to inhibit the formation of oral dual biofilms, a critical contributor dental caries and other diseases. The purpose this was evaluate effectiveness containing tt-farnesol, quercetin, theobromine in inhibiting mixed Streptococcus mutans Candida albicans biofilms. Mixed biofilms UA159 SC5314 were grown 96-well plates specialized culture medium. Approximately 0.2 mL experimental with A-1.5% or B-4.5% concentrations separately added wells using disposable applicator, vehicle varnish (lacking bioactives) serving as control. Biofilms incubated at 37 °C 5% CO2 for 24 h. Microbial viability determined terms colony-forming units per milliliter (CFU/mL), biofilm morphology evaluated qualitatively via scanning electron microscopy (SEM). Statistical analyses performed ANOVA/Tukey tests significance level. Varnishes A B achieved significant reductions microbial populations within (p < 0.05) compared control (C). SEM imaging revealed marked structural disruptions validating quantitative results. Higher demonstrated enhanced inhibitory effects. Bioactive enriched theobromine, tt-farnesol represent novel effective strategy development, offering promising advancement preventive dentistry.

Language: Английский

Citations

0
Antibacterial Effects of Er:YAG Laser Irradiation on Candida–Streptococcal Biofilms DOI Creative Commons
Zuzanna Grzech-Leśniak,

Jakub Pyrkosz,

Jagoda Szwach

et al.

Life, Journal Year: 2025, Volume and Issue: 15(3), P. 474 - 474

Published: March 16, 2025

In contemporary dentistry, laser-based interventions have become one of the mainstays care for patients with oral biofilm diseases, such as candidiasis, periodontal disease and peri-implantitis. The purpose this study was to evaluate effectiveness Er:YAG laser (LightWalker, Ljubljana, Fotona, Slovenia) irradiation at varying irradiance levels (T1: 11.3 W/cm2 T2: 120.54 W/cm2) on microbial viability in single- dual-species models, focusing Candida albicans, glabrata Streptococcus mutans, address challenges managing complex biofilms clinically relevant settings. results showed substantial reduction, C. albicans being most susceptible microorganism (93–99.9%), while exhibited marked resistance higher levels. Interestingly, S. mutans demonstrated reductions based composition, highlighting influence interactions. This concluded that effectively reduced viability, its efficacy depending composition These findings highlight need tailored erbium parameters optimize clinical outcomes, underscoring individualized polymicrobial management, particularly peri-implant therapies.

Language: Английский

Citations

0
Investigation Ability of Local Micrococcus Luteus to Produce Biofilm (Sustainable Exopolysaccharide) and Studying Its Optimum Production Conditions DOI Open Access

Karam Khalid Chalop,

Ibtissem Moussa

IOP Conference Series Earth and Environmental Science, Journal Year: 2025, Volume and Issue: 1487(1), P. 012131 - 012131

Published: April 1, 2025

Abstract The study aimed to detection the ability of local Micrococcus luteus ML1 produce Biofilm (Sustainable exopolysaccharides) & determining some optimal conditions for Its production by studying six factors affecting production(temperature, pH, inoculame volume, incubation time, sucrouse percentage, Shaking speed) in laboratories Iraqi Ministry Agriculture / Department Crop Protection. Congo red agar test Elisa Technique designated investigate isolates biofilm, as isolate proved its exopolysaccharides indicated colour change medium a result formation biofilm this environment, also show moderate microtiterplates. bacteria gave highest extracellular polysaccharides ma45trix at temperature 35°C, an period 168 hours, pH 7, sucrose concentration 15%, inoculum volume 6 ml, and shaking speed 75 rpm.

Language: Английский

Citations

0
Trans, Trans-Farnesol Enhances the Anti-Bacterial and Anti-Biofilm Effect of Arachidonic Acid on the Cariogenic Bacteria Streptococcus mutans and Streptococcus sobrinus DOI Open Access

Farah Haj-Yahya,

Doron Steinberg,

Ronit Vogt Sionov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11770 - 11770

Published: Nov. 1, 2024

Background: Streptococcus mutans and sobrinus are Gram-positive bacteria involved in the development of dental caries, as they able to form biofilms on tooth enamel, ferment sugars into acids, survive under acidic conditions. This ultimately leads a local lowering pH value surface, which causes enamel cavities. Hypothesis: One measure reduce caries is limit growth cariogenic by using two anti-bacterial agents with different mechanisms action. The hypothesis this study was that activity ω-6 polyunsaturated arachidonic acid (AA) against S. can be enhanced sesquiterpene alcohol trans, trans-farnesol (t,t-farnesol). Methods: single combined treatment determined checkerboard assay. Bacterial viability assessed live/dead SYTO 9/propidium iodide (PI) staining flow cytometry. Anti-biofilm MTT metabolic assay, crystal violet biofilm biomass, 9/PI spinning disk confocal microscopy (SDCM) high-resolution scanning electron (HR-SEM). Results: t,t-Farnesol lowered minimum inhibitory concentration (MIC) (MBIC) AA at sub-MICs. reduced preformed mature biofilms, while t,t-farnesol had no significant effect. effect t,t-farnesol/AA further evidenced increased PI uptake, indicating membrane perforation. anti-biofilm verified SDCM HR-SEM. Gene expression studies showed some biofilm-related genes. Conclusions: Altogether, our suggests potential use naturally occurring compounds for preventing formation sobrinus. These findings have implications prevention.

Language: Английский

Citations

1