Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors DOI
Andrea Colautti, Elisabetta Orecchia, Giuseppe Comi

et al.

Journal of Bacteriology, Journal Year: 2022, Volume and Issue: 204(11)

Published: Oct. 26, 2022

To date, several studies have reported an alarming increase in pathogen resistance to current antibiotic therapies and treatments. Therefore, the search for effective alternatives counter their spread onset of infections is becoming increasingly important. In this regard, microorganisms former Lactobacillus genus demonstrated ability reduce virulence pathogens. addition production bioactive substances, self- coaggregation, substrate competition, lactobacilli influence gene expression by downregulating genes associated with As many vivo vitro trials, counteract inhibit various factors that favor pathogens, including toxins, biofilm formation, host cell adhesion invasion, downregulation linked quorum sensing. The aim review summarize on inhibition lactobacilli, important microbial group well known industrial medical fields technological probiotic properties benefit human hosts potential provide aid fight against pathogens besides use therapies. Further research could lead identification new strains that, alleviating adverse effects, improve efficacy or play preventive role reducing if regularly taken.

Language: Английский

Probiogenomic analysis of Lactiplantibacillus plantarum SPS109: A potential GABA-producing and cholesterol-lowering probiotic strain DOI Creative Commons

Nutwadee Chintakovid,

Kamonnut Singkhamanan, Thunchanok Yaikhan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33823 - e33823

Published: June 28, 2024

Lactiplantibacillus plantarum SPS109, an isolated strain of lactic acid bacteria (LAB) from fermented foods, showed remarkable potential as a probiotic with dual capabilities in γ-aminobutyric (GABA) production and cholesterol reduction. This study employs genomic comparative analyses to search into the strain's genetic profile, safety features, attributes. The assessment reveals absence virulence factors antimicrobial resistance genes, while genome uncovers bacteriocin-related elements, including sactipeptides cluster for putative plantaricins, strengthening its ability combat diverse pathogens. Pangenome analysis revealed unique specifically lcnD bcrA, distinguishing SPS109 four other L. strains producing GABA. In addition, emphasizes distinctive two GABA-related genes responsible GABA bile tolerance gene (cbh) crucial Additionally, highlights several properties, stress tolerance, vitamin production, antioxidant activity. summary, emerges promising candidate versatile applications food beverage industries, supported by features profile.

Language: Английский

Citations

6
Novel Strategies for Efficient Production and Delivery of Live Biotherapeutics and Biotechnological Uses of Lactococcus lactis: The Lactic Acid Bacterium Model DOI Creative Commons

Laísa Macedo Tavares,

Luís Cláudio Lima de Jesus, Tales Fernando da Silva

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2020, Volume and Issue: 8

Published: Nov. 4, 2020

Lactic Acid Bacteria (LAB) are traditionally used in fermentation and food preservation processes recognized as safe for consumption. Recently, they have attracted attention due to their health-promoting properties; many species already widely probiotics treatment or prevention of various medical conditions, including inflammatory bowel diseases, infections, autoimmune disorders. Some LAB, especially Lactococcus lactis, been engineered live vehicles delivery DNA vaccines production therapeutic biomolecules. Here we summarize work on engineering with emphasis the model L. lactis. We review expression systems heterologous proteins spp. its use a system biotherapeutics using eukaryotic cell machinery. included examples molecules produced by these platforms application clinical also present CRISPR-Cas approach novel methodology development optimization food-grade useful substances, detail methods improve LAB gastrointestinal tract. Finally, discuss perspectives applications recombinant LABs involving animal studies human trials, touch main safety issues that need be taken into account so bioengineered versions generally organisms will considered acceptable use.

Language: Английский

Citations

42
Lacticaseibacillus rhamnosus FM9 and Limosilactobacillus fermentum Y57 Are as Effective as Statins at Improving Blood Lipid Profile in High Cholesterol, High-Fat Diet Model in Male Wistar Rats DOI Open Access

Hamza Zafar,

Noor ul Ain, Abdulrahman Alshammari

et al.

Nutrients, Journal Year: 2022, Volume and Issue: 14(8), P. 1654 - 1654

Published: April 15, 2022

Elevated serum cholesterol is a major risk factor for coronary heart diseases. Some Lactobacillus strains with cholesterol-lowering potential have been isolated from artisanal food products. The purpose of this study was to isolate probiotic traditional yoghurt (dahi) and yogurt milk (lassi) investigate the impact these on blood lipid profile anti-obesity effect in high fat diet model Wistar rats. Eight candidate were chosen based vitro features reduction ability. By 16S rDNA sequencing, identified as Limosilactibacillus fermentum FM6, L. FM16, FM12, Lacticaseibacillus rhamnosus FM9, Y55, Y57, Y59, Y63. safety investigated by feeding 2 × 108 CFU/mL saline water 28 days rat model. No bacterial translocation or any other adverse effects observed animals after administration water, which indicates strains. probiotics evaluated male rats using high-fat, high-cholesterol (HFCD) For 30 days, fed CFU/mL/rat/day, addition fat, diet. various compared those statin. All showed improvement total cholesterol, LDL, HDL, triglycerides, weight gain. Serum levels reduced 9% 8% FM9 respectively, 5% statin-treated group. HDL significantly improved 46 44% 46% Compared group, Y57 LDL almost twofold. These findings show that can improve profiles effectively statins Furthermore, probiotic-fed groups helped control HFCD, indicating possible be used develop products supplements treat ischemic diseases management. Clinical trials, however, are required validate findings.

Language: Английский

Citations

26
Use of genetically modified lactic acid bacteria and bifidobacteria as live delivery vectors for human and animal health DOI Creative Commons
Romina Levit, Naima G. Cortes‐Perez, Alejandra de Moreno de LeBlanc

et al.

Gut Microbes, Journal Year: 2022, Volume and Issue: 14(1)

Published: Aug. 12, 2022

There is now strong evidence to support the interest in using lactic acid bacteria (LAB)in particular, strains of lactococci and lactobacilli, as well bifidobacteria, for development new live vectors human animal health purposes. LAB are Gram-positive that have been used millennia production fermented foods. In addition, numerous studies shown genetically modified bifodobacteria can induce a systemic mucosal immune response against certain antigens when administered mucosally. They therefore good candidates delivery strategies attractive alternatives vaccines based on attenuated pathogenic whose use presents risks. This article reviews most recent research advances bifidobacteria health.

Language: Английский

Citations

26
Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors DOI
Andrea Colautti, Elisabetta Orecchia, Giuseppe Comi

et al.

Journal of Bacteriology, Journal Year: 2022, Volume and Issue: 204(11)

Published: Oct. 26, 2022

To date, several studies have reported an alarming increase in pathogen resistance to current antibiotic therapies and treatments. Therefore, the search for effective alternatives counter their spread onset of infections is becoming increasingly important. In this regard, microorganisms former Lactobacillus genus demonstrated ability reduce virulence pathogens. addition production bioactive substances, self- coaggregation, substrate competition, lactobacilli influence gene expression by downregulating genes associated with As many vivo vitro trials, counteract inhibit various factors that favor pathogens, including toxins, biofilm formation, host cell adhesion invasion, downregulation linked quorum sensing. The aim review summarize on inhibition lactobacilli, important microbial group well known industrial medical fields technological probiotic properties benefit human hosts potential provide aid fight against pathogens besides use therapies. Further research could lead identification new strains that, alleviating adverse effects, improve efficacy or play preventive role reducing if regularly taken.

Language: Английский

Citations

26