Role of Insect Gut Microbiota in Pesticide Degradation: A Review

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 3, 2022

Insect pests cause significant agricultural and economic losses to crops worldwide due their destructive activities. Pesticides are designed be poisonous intentionally released into the environment combat menace caused by these noxious pests. To survive, insects can resist toxic substances introduced humans in form of pesticides. According recent findings, microbes that live insect as symbionts have recently been found protect hosts against toxins. Symbioses formed between various microbes, a defensive mechanism pathogens Insects’ guts provide unique conditions for microbial colonization, resident bacteria deliver numerous benefits hosts. Insects vary significantly reliance on gut basic functions. digestive tracts very different shape chemical properties, which big impact structure composition community. microbiota has contribute feeding, parasite pathogen protection, immune response modulation, pesticide breakdown. The current review will examine roles detoxification mechanisms behind development resistance better understand detoxifying agriculturally pest insects, we provided comprehensive information regarding role

Language: Английский

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Culturable bacteria associated with Anastrepha fraterculus sp. 1: in search of nitrogen‐fixing symbionts with biotechnological potential

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria protection nutrient assimilation flies, particularly importance biological fixation nitrogen (diazotrophy). The presence diazotrophic A. sp. 1 has been demonstrated through molecular, culture-independent methods. This study aimed to characterize composition diversity culturable males from different origins, explore their metabolic roles, focusing on bacteria. Three male groups were studied: wild-caught (WW), lab-reared wild larvae (WL), lab-colony raised (LL). Gut collected characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL NFb). Phylogenetic analysis mapped across bacterial collection, while biochemical profiling ARDRA (Amplified rDNA Restriction Analysis) used quickly differentiate PCR testing for nifH gene, associated fixation, was also performed. Bacterial highest WW, followed by WL, lowest LL. In LL Enterobacter most frequent genus, Klebsiella dominated WW. Among 20 SIL+ isolates identified, 10 came 9 One these (Enterobacter sp.) tested as supplement adult diet, without showing beneficial effect pheromone calling behavior. NFb+; two had gene. effective rapid diazotroph discrimination. These findings highlight symbiotic eco-friendly management strategies like sterile insect technique (SIT). By bacteria, protein requirements artificial diets could be reduced, cutting costs improving affordability SIT programs.

Language: Английский

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Cladosporium—Insect Relationships

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(1), P. 78 - 78

Published: Jan. 19, 2024

The range of interactions between

Language: Английский

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The Role of Bacterial Symbionts in Triatomines: An Evolutionary Perspective

Microorganisms, Journal Year: 2020, Volume and Issue: 8(9), P. 1438 - 1438

Published: Sept. 19, 2020

Insects have established mutualistic symbiotic interactions with microorganisms that are beneficial to both host and symbiont. Many insects exploited these symbioses diversify expand their ecological ranges. In the Hemiptera (i.e., aphids, cicadas, true bugs), evolved obligatory essential (primary symbionts) facultative symbionts (secondary symbionts). Primary usually intracellular found in specialized diets such as obligate hematophagy or phytophagy. Most Heteroptera (true however, gastrointestinal (GI) tract extracellular functions analogous primary endosymbionts. The triatomines, vectors of human parasite, Trypanosoma cruzi. A description small GI microbiota richness was based on a few culturable first described almost century ago. growing literature describes more complex between triatomines bacteria properties characteristic secondary symbionts. this review, we provide an evolutionary perspective Hemiptera, illustrating context may drive evolution triatomines. We highlight diversity triatomine microbiota, bacterial taxa potential be symbionts, unique characteristics triatomine-bacteria symbioses, among trypanosomes,

Language: Английский

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Relationship Between the Host Plant Range of Insects and Symbiont Bacteria

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 189 - 189

Published: Jan. 17, 2025

The evolution of phytophagous insects has resulted in the development feeding specializations that are unique to this group. majority current research on insect palatability concentrated aspects ecology and biology, with relatively little attention paid role gut symbiotic bacteria. Symbiont bacteria have a close relationship their hosts perform range functions. This aimed investigate between host plant A synthesis extant literature intestinal commensal monophagous, oligophagous, polyphagous tephritids revealed no evidence positive correlation diversity larval microbial species. symbionts same species were observed exhibit discrepancies different sources, which attributed variations multiple environmental factors. However, following beta analysis, monophagy demonstrated lowest level variation bacteria, while exhibited greatest community variation. In light these findings, study proposes hypothesis exclusive or closely related provide oligophagy stable core colony over long evolutionary periods. flora is associated adaptations monophagous oligophagous tephritids, including nutritional detoxification contrast polyphagy, whose dominant varies environments. Our requires further refinement data as number samples currently limited.

Language: Английский

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Gut bacterial population and community dynamics following adult emergence in pest tephritid fruit flies

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Aug. 22, 2023

Abstract Gut microbiota are important contributors to insect success. Host-microbe interactions dynamic and can change as hosts age and/or encounter different environments. A turning point in these relationships the transition from immature adult life stages, particularly for holometabolous insects where there is radical restructuring of gut. Improved knowledge population community dynamics gut microbiomes upon emergence inform drivers assembly physiological aspects host-microbe interactions. Here, we evaluated bacterial communities pest tephritid species melon fly ( Zeugodacus cucurbitae ) Medditeranean fruit (medfly, Ceratitis capitata associated with pupae stage timepoints immediately following eclosion. We used a combination culturing determine cultivatable titers, qPCR 16S-rRNA SSU copy numbers, 16S V4 sequencing changes communities. Both revealed that populations declined by 10 100-fold followed recovery within 24 h Titers reached ~ 7 CFUs (~ 8 rRNA copies) week post-emergence. also observed concurrent amplicon sequence variance (ASVs), ASV composition differed overtime both medfly adults at timepoints. Medfly, particular, had microbiome compositions each timepoint, indicating greater levels variation before stabilization. These results demonstrate experience period flux emergence, biomass makeup undergoes dramatic shifts. The document suggest plasticity may be specific periods pliable introduce establish new microbial strains host.

Language: Английский

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Metagenomic Survey of the Highly Polyphagous Anastrepha ludens Developing in Ancestral and Exotic Hosts Reveals the Lack of a Stable Microbiota in Larvae and the Strong Influence of Metamorphosis on Adult Gut Microbiota

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 2, 2021

We studied the microbiota of a highly polyphagous insect, Anastrepha ludens (Diptera: Tephritidae), developing in six its hosts, including two ancestral ( Casimiroa edulis and C. greggii ), three exotic Mangifera indica cv. Ataulfo, Prunus persica Criollo, Citrus x aurantium ) one occasional host Capsicum pubescens Manzano), that is only used when extreme drought conditions limit fruiting by common hosts. One hosts (“criollo” peach) rife with polyphenols capsaicinoids exerting high fitness costs on larvae. pursued following questions: (1) How microbial composition larval food related to adult microbiota, what does this tell us about transience stability species’ gut microbiota? (2) metamorphosis affect surveyed pulp each fruit, as well larvae flies found A. lacks stable since it was invariably associated plant species also different from indicating filters out much present The males females similar between them, independent dominated bacteria within Enterobacteriaceae. case “toxic” enriched potentially deleterious genera were less abundant other In contrast, several bacterial groups can be beneficial for development. report first time presence Arcobacteraceae family stemming . Based our findings, we conclude changes food-associated dictate major suggesting most originated food.

Language: Английский

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Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 16, 2020

Larval diets used for artificial rearing can have a significant effect on insect biology. The Queensland fruit fly (aka 'Qfly'), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is one of the greatest challenges growers in Australia. sterile technique (SIT) being developed to manage outbreaks regions that remain free Qfly and reduce populations where this species endemic. Factory scale essential SIT; however, larval are known affect microbiome Qfly, which may then performance. In study, high-throughput Illumina sequencing was assess colonies reared, five generations from nature, two common (carrot gel). At generation (G5), assessed larvae, pupae, adult males females standard quality control parameters were together with additional performance measures mating propensity survival under nutritional stress. genus level, bacterial communities significantly different between reared diets. However, converged at Phyla family taxonomic levels. Interestingly, pathogenic genera (e.g., Morganella, Citrobacter, Providencia, Burkholderia) highly abundant all developmental stages gel diet, when compared carrot diet. Despite abundance these commonly genera, greater percentage egg hatching, heavier pupal weight higher fliers found Mating stress similar had been Overall, our findings demonstrate diet strongly influences likely downstream effects flies released SIT programs.

Language: Английский

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Dynamics of the Queensland Fruit Fly Microbiome through the Transition from Nature to an Established Laboratory Colony

Microorganisms, Journal Year: 2022, Volume and Issue: 10(2), P. 291 - 291

Published: Jan. 26, 2022

The transition from nature to laboratory or mass rearing can impose significant physiological and evolutionary impact on insects. Queensland fruit fly (also known as 'Qfly'), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is a serious economic pest that presents major challenges for horticulture industries in Australia. sterile insect technique (SIT) being developed manage outbreaks regions remain free of Qfly suppress populations where this species endemic. biology intimately connected its microbiome. Therefore, changes the microbiome occur through domestication have implications SIT. There are numerous studies larvae adults, but there little information how colonies established. In study, high-throughput Illumina sequencing was used assess reared wild larvae, collected fruit, five generations, gel-based larval diet. Beta diversity analysis showed bacterial communities Generation 5 (G5) clustered separately earlier generations. At genus level, were significantly different between generations mostly altered at G5. However, found similar phyla family taxonomic levels. We observed high abundance Morganella Burkholderia level pupal stages respectively G5, these not detected Overall, our findings demonstrate process strongly affects prompts questions about functional relationship microbiome, well performance insects been domesticated mass-reared SIT programs.

Language: Английский

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Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Oct. 6, 2020

Abstract Bactrocera tryoni (Froggatt), the Queensland fruit fly (Qfly), is a highly polyphagous tephritid that widespread in Eastern Australia. Qfly physiology closely linked with its fungal associates, particular relationship between nutrition and yeast or yeast-like fungi. Despite animal-associated fungi typically occurring multi-species communities, studies have predominately involved culture characterisation of single isolates. Further, only two investigated communities associated Qfly, both used culture-dependant techniques overlook non-culturable hence under-represent, provide biased interpretation of, overall community. In order to explore potentially hidden diversity complexity within mycobiome, we culture-independent, high-throughput Illumina sequencing comprehensively, holistically characterized community larvae overcome bias. We collected from range hosts along east coast Australia, all had mycobiome dominated by ascomycetes. The most abundant taxa belonged genera Pichia (43%), Candida (20%), Hanseniaspora (10%), Zygosaccharomyces (11%) Penicillium (7%). also hosts, found strong degree overlap host suggesting these are intimately inter-connected. Our data suggests larval acquired surrounding flesh. It likely physiological benefits exposure primarily due consumption fungi, not through syntrophy/symbiosis insect ‘host’.

Language: Английский

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