Microbiomes of Two Pest Fly Species of Pennsylvania Mushroom Houses

Insects, Journal Year: 2024, Volume and Issue: 15(7), P. 525 - 525

Published: July 12, 2024

Mushroom cultivation vastly improves the yield of mushrooms under optimized, controlled conditions, but may be susceptible to opportunistic colonization by pest species that can establish themselves, as well pathogens and pests they transmit. Here, we describe our investigation into bacterial communities adult

Language: Английский

---

Utilizing full-length 16S rRNA sequencing to assess the impact of diet formulation and age on targeted gut microbiome colonization in laboratory and mass-reared Mediterranean fruit flies

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract Insect gut microbiomes have important roles in overall host health and how hosts function the environment. In laboratory mass-reared insects, can differ composition compared to wild conspecifics. For fruit flies, like Mediterranean fly (medfly; Ceratitis capitata ), these changes influence male performance behavior. Overall, understanding factors that ability of bacteria colonize is an for establishment lost or novel microbiota into insects. The goal this study was evaluate age diet inoculation method influenced bacterial medfly. We used Enterobacter strain with antibiotic resistance coupled it full-length PacBio 16S rRNA sequencing track a specific isolates under different adult dietary conditions. also two longstanding reared lines medfly our study. Our results identified had strong interaction age. Host fed liquid target were able be colonized regardless age, but those slurry-based separate water source more resilient. This consistent both rearing corroborated strain, revealed some species/strain-level variation sequences associated flies. Additionally, illustrates long-read may afford improved characterization species- strain-level distribution Enterobacteriaceae Importance: Insects form intimate relationships microorganisms help facilitate several roles. goals microbial (medfly), pest species throughout world. Mass-reared insects sterile insect technique often possess substantially from which impact their control contexts. Here, we show liquid-based formulations utilized manipulate Furthermore, using near metabarcoding sequencing, uncovered diversity not immediately obvious other approaches. notable finding, as suggests approaches marked improvements taxa fewer hypervariable regions at approximately same cost. provide new avenues exploring interrogating medfly-microbiome interactions.

Language: Английский

---

Electrophysiological and Behavioral Responses of Virgin Female Bactrocera tryoni to Microbial Volatiles from Enterobacteriaceae

Microorganisms, Journal Year: 2023, Volume and Issue: 11(7), P. 1643 - 1643

Published: June 23, 2023

The Queensland fruit fly (Bactrocera tryoni) is a major polyphagous pest widespread in Australia and several Pacific Islands. Bacteria present on the host plant phyllosphere supply proteins, essential for egg development female sexual maturity. We investigated role of microbial volatile organic compounds (MVOCs) emitted by Enterobacteriaceae commonly found gut attracting virgin females. were cultured artificial media natural fruits, at various pH, MVOCs collected using different headspace absorbent materials. olfactory responses females to bacterial assessed via electrophysiology behavioral assays. production was strongly influenced qualitatively strain type media, it semi-quantitatively varied with pH time. Klebsiella oxytoca invoked strongest antennal response most attractive. Among identified triggering an response, D-limonene 2-nonanone both significantly behaviorally attractive, whereas phenol, nonanal, isoamyl alcohol, some pyrazines appeared be repulsive. This study deepens our understanding chemical ecology between flies their symbionts paves way novel synthetic lures based specifically targeting

Language: Английский

---

Cladosporium - Insect Relationships

Published: Dec. 21, 2023

The range of interactions between Cladosporium, an ubiquitous fungal genus, and insects, a class including about 60% the animal species, is extremely diverse. broad case history antagonism mutualism connecting Cladosporium insects reviewed in this paper based on examination available literature. Certain strains establish direct with pest or beneficial indirectly influence them through their endophytic development plants. Entomopathogenicity often connected to production toxic secondary metabolites, although there where these compounds have been reported favor pollinators attraction, suggesting important role angiosperm reproduction. Other relationships include mycophagy, which other hand may reflect ecological advantage for adaptable fungi using as carriers spreading environment. Several species colonize insect structures, such galleries ambrosia beetles, leaf-rolls attelabid weevils galls formed by cecidomyid midges, playing still uncertain symbiotic role. Finally, occurrence gut several has intriguing implications management, also considering that some proven be able degrade insecticides. These especially deserve further investigation understand impact control measures strategies preserve insects.

Language: Английский

---

Stage correlation of symbiotic bacterial community and function in the development of litchi bugs (Hemiptera: Tessaratomidae)

Antonie van Leeuwenhoek, Journal Year: 2021, Volume and Issue: 115(1), P. 125 - 139

Published: Nov. 29, 2021

Language: Английский

---