Changes in the intestinal microbiota of farmed northern sheatfish (Silurus soldatovi) associated with natural bacterial infection DOI Creative Commons

Zhongjie Zan,

Qing Mao, Zhuoran Han

et al.

Journal of the World Aquaculture Society, Journal Year: 2023, Volume and Issue: 54(6), P. 1575 - 1591

Published: July 2, 2023

Abstract An acute infectious disease of northern sheatfish ( Silurus soldatovi ) was found in a commercial aquaculture pond Tianjin, China. Herein, two dominant bacterial strains were isolated from naturally diseased and identified as Aeromonas veronii Vibrio cholerae . Seven healthy seven fish that collected the used for gut microbiota analysis by high‐throughput 16S rRNA gene sequencing. The α‐diversity indices (ACE, Chao 1, Shannon) did not differ significantly between p > 0.05), but significant separation observed them when β ‐ diversity population performed. demonstrated elevation relative abundances , Blautia Megamonas decrease Cetobacterium Bacteroides Chryseolinea Ralstonia Pseudomonas compared with < 0.05). Linear discriminant effect size showed microbial marker species order Aeromonadales, family Aeromonadaceae, genus functional profiles community predicted using PICRUSt2 indicated disturbance caused alteration its fish.

Language: Английский

Teleost skin microbiome: An intimate interplay between the environment and the host immunity DOI
Liang‐Chun Wang, Li-Hsuan Chen,

Yu‐Che Chiu

et al.

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 139, P. 108869 - 108869

Published: June 5, 2023

Language: Английский

Citations

10
Effect of intestinal microbiota on growth rate of Babylonia areolata DOI Creative Commons
Wang Zhao,

Xingmei Huang,

Haipeng Qin

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0322985 - e0322985

Published: May 7, 2025

To analyze the differences in intestinal microbiota structure of different growth rates Babylonia areolata , sequencing V3-V4 regions 16S rDNA from samples was performed. Evaluation richness by calculating Shannon index, Simpson index and Chao1 showed that community diversity B. changed for rates. There are fast rate (FG) slow (SG) groups. A total 315,294 reads were obtained 6 samples, 17 phyla identified RDP classifier. After data standardization, dominant FG SG Firmicutes Proteobacteria, Bacteroidetes, 50.23%, 39.31% 8.39% group, 79.97%, 12.05% 6.21% respectively. The distribution group is relatively uniform, but Mycoplasma genus accounting about 63%. At level, compared to exhibited a significant increase abundance Exiguobacterium Vibrio Escherichia-Shigella while Citrobacter Phascolaretobacterum significantly reduced. These findings provide novel information studying with rates, foundation future research on bacterial factors may affect cultivation .

Language: Английский

Citations

0
The supplementation of a prebiotic improves the microbial community in the gut and the skin of Atlantic salmon (Salmo salar) DOI Creative Commons

Simon Baumgärtner,

Jack E. James, Amy Ellison

et al.

Aquaculture Reports, Journal Year: 2022, Volume and Issue: 25, P. 101204 - 101204

Published: June 15, 2022

Aquaculture growth is hindered by an increasing number of challenges, primarily infectious diseases and inappropriate or unsustainable fish nutrition. Hence it critical to develop novel prevention strategies minimise pharmaceutical interventions. Nutritional challenges the health could be improved managing their microbial communities. Microbiomes can play a crucial role in physiology, particularly digestion, metabolizing largely indigestible feed components for host synthesis essential micronutrients. Beyond nutritional role, microbiomes are considered first line defence against pathogens. In this study, prebiotic mix (Selectovit), composed 1,3/1,6-beta glucans, mannan-oligosaccharides, nucleic acids, nucleotides, medium chain fatty acids single was tested at different inclusion levels (0.0; 0.5; 1.0; 2.0 g/kg) diet Atlantic salmon (Salmo salar). Using experimental trials 16 S rRNA microbiome profiling, impact blend on community within both gastrointestinal tract skin assessed. Overall, supplement showed no significant growth. However, we clearly demonstrate that significantly manipulate distal intestine skin. Several potential beneficial bacteria such as Bacillus Mycoplasma spp. were more abundant prebiotic-fed groups compared control. contrast, putative pathogenic less fed blend. Interestingly, induced changes than gut. There growing evidence highly complex interactions between communities digestive system external mucosa, with immune system. Further research field lead creation bacterial biomarkers new non-invasive monitoring.

Language: Английский

Citations

14
Skin Microbial Community Associated to Strawberry Disease in Farmed Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) DOI Creative Commons

Alda Pardo,

Alejandro Villasante, Jaime Romero

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(1), P. 217 - 217

Published: Jan. 21, 2024

Aquaculture plays a crucial role in addressing the growing global demand for food. However, diseases associated with intensive aquaculture practices, especially those affecting skin, can present significant challenges to both fish health and industry as whole. Strawberry disease (SD), also known red-mark syndrome, is persistent non-lethal skin condition observed Rainbow Trout (

Language: Английский

Citations

2
Extracellular matrix (ECM) pathway involved in skin immune response of Cynoglossus semilaevis upon Vibrio vulnificus infection DOI Creative Commons

Houfu Liu,

Ge Jin,

Ruoxuan Wang

et al.

Water Biology and Security, Journal Year: 2024, Volume and Issue: 3(3), P. 100277 - 100277

Published: June 19, 2024

Half-smooth tongue sole (Cynoglossus semilaevis) is regarded as a significant commercial marine fish species in China, and frequent outbreaks of vibriosis has led to substantial economic losses. In this study, we investigated the molecular mechanisms underlying involvement extracellular matrix (ECM) signaling pathway skin immune response C. semilaevis infected with Vibrio vulnificus. The results showed that most differentially expressed proteins (DEPs) were identified through iTRAQ proteome analysis, ten ECM-related screened at 12 36 h post-infection (hpi). Notably, several DEPs associated ECM including HSPG, FN, COCH, LAMB1, LAMC1, TSPN, COL6A1, AMBP, HX ITGA6 tested for their expression transcriptional level during V. vulnificus infection using qRT-PCR assay. analysis protein-protein interaction (PPI) integrin exhibited obvious interactions among DEPs. Furthermore, spatio-temporal Csitga6 gene was highest skin, gill, muscle, spleen, but lower liver, kidney intestine. To our knowledge, first report on infection, provides reference further study mechanism mucosal fish.

Language: Английский

Citations

2
Sexual dimorphism in fish innate immunity: a functional and transcriptional study in yellowtail kingfish DOI Creative Commons
Yulema Valero, Carlos Hurtado, Luís Mercado

et al.

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 154, P. 109921 - 109921

Published: Sept. 23, 2024

Language: Английский

Citations

2
Infection and antibiotic treatment have prolonged effects on gut microbiota, muscle and hepatic fatty acids in rainbow trout (Oncorhynchus mykiss) DOI Open Access
Aleksey Parshukov, Natalia Fokina, Irina Sukhovskaya

et al.

Journal of Applied Microbiology, Journal Year: 2022, Volume and Issue: 133(3), P. 1709 - 1724

Published: June 19, 2022

The aim of the present study was to investigate gastrointestinal (GI) microbiota and bacterium-specific fatty acid occurrence in muscle hepatic lipids rainbow trout Oncorhynchus mykiss (Walbaum, 1792), both healthy those naturally infected with bacterial pathogens.From June 2017 (L1) September 2018 (L8), 74 specimens (with an average weight from 139.2 ± 7.1 g 2191.7 85.1 (L8)) were studied. Amplicon sequencing targeted V3-V4 region 16S rRNA gene fragments is used for identification taxonomic composition gut communities. Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes Fusobacteria major phyla. Besides behavioural physiological manifestations mixed disease (yersiniosis, pseudomonosis flavobacteriosis), some disorders induced by infection followed antibiotic treatment detected host organism, including (1) a progressive decrease content odd-chain saturated acids origin within lipid molecules (2) abnormalities GI tract microbiota, such as elimination LAB certain taxa, particularly Mycoplasmataceae.The flora varied principally due Mycoplasmataceae Lactobacillaceae, which could be considered search bioindicators. specific bacterium-derived incorporated into seems related prevalence their deficit regarded early warning sign disturbance.Our results demonstrated that infectious reared species can cause pertinent imbalance reduce abundance acids. This useful sustainable aquaculture industry development indication technologies rapid diagnosis.

Language: Английский

Citations

11
First Evaluation of Associated Gut Microbiota in Wild Thick-Lipped Grey Mullets (Chelon labrosus, Risso 1827) DOI Creative Commons
Jorge García‐Márquez, Isabel M. Cerezo, Félix L. Figueroa

et al.

Fishes, Journal Year: 2022, Volume and Issue: 7(4), P. 209 - 209

Published: Aug. 19, 2022

This study aimed to characterize the intestinal microbiota of wild thick-lipped grey mullets (Chelon labrosus) and explore its potential functionality on host. Intestinal contents anterior posterior sections from fish were collected DNA was extracted. Subsequently, V3–V4 regions 16S rRNA sequenced using Illumina technology results analyzed by bioinformatics pipeline. The functional profile microbial community PICRUSt software. Shannon Simpson diversity indices significantly higher in section specimens. overall taxonomic composition suggests a certain homogeneity intestine heterogeneity section. Due this, no statistical differences detected at any level among both sections. Predicted functions showed most abundant those related amino acid metabolism, carbohydrate energy membrane transport, cell replication repair. Furthermore, analysis revealed genes elimination environmental toxins.

Language: Английский

Citations

11
The haematology of clinically healthy, farmed juvenile Asian seabass (Lates calcarifer Bloch)—reference intervals, and indicators of subclinical disease DOI Creative Commons
Xian Zhe Chew, Susan Gibson‐Kueh

Journal of Fish Diseases, Journal Year: 2023, Volume and Issue: 46(10), P. 1109 - 1124

Published: July 14, 2023

This study establishes the blood reference intervals (RIs) for clinically healthy and farmed juvenile Asian seabass (Lates calcarifer), within 4-6 weeks after stocking into flow-through, marine aquaculture systems. The 90% percentile RIs (n = 156, mean bodyweight 41.8 g) are as follows: glucose (GLU) 2.4-11.3 mmol/L, haematocrit (Hct) 18.9%-39.2%, haemoglobin concentration (Hb) 56.0-85.0 g/L, total plasma protein (TPP) 56.0-77.0 red cell (RBC) count 4.1-11.2 × 1012 /L, white (WBC) 5.3-69.9 109 lymphocytes 4.7-51.4 monocytes 0.3-16.2 /L heterophils 0.6-8.4 /L. Pearson's method analysis showed weak but significantly positive correlations between fish Hct, Hb, TPP RBC (p < 0.05). Histopathology of 42 156 used to derive RIs, with values range, did not exhibit any abnormal pathology. In contrast, histopathology from a different group L. calcarifer 72, 31.3 falling outside these established that 25% had severe, chronic granulomatous enteritis, 13% severely depleted lipid stores in their liver. Point biserial correlation this second 72 elevated WBC, monocyte heterophil counts reduced Hct levels associated 0.05) occurrence enteritis Reduced GLU were liver 0.05), corroborating period malnutrition. is among first establish healthy, detection subclinical diseases support early intervention.

Language: Английский

Citations

5
Effects of Chlorella fusca-supplemented diet on intestinal microbiota and gene expression related to metabolism, stress, and immune response in Chelon labrosus DOI
Jorge García‐Márquez, Daniel Álvarez-Torres, Isabel M. Cerezo

et al.

Algal Research, Journal Year: 2023, Volume and Issue: 77, P. 103362 - 103362

Published: Dec. 12, 2023

Language: Английский

Citations

5