Food Antioxidants and Their Interaction with Human Proteins

Antioxidants, Journal Year: 2023, Volume and Issue: 12(4), P. 815 - 815

Published: March 27, 2023

Common to all biological systems and living organisms are molecular interactions, which may lead specific physiological events. Most often, a cascade of events occurs, establishing an equilibrium between possibly competing and/or synergistic processes. Biochemical pathways that sustain life depend on multiple intrinsic extrinsic factors contributing aging diseases. This article deals with food antioxidants human proteins from the circulation, their interaction, effect structure, properties, function antioxidant-bound proteins, possible impact complex formation antioxidants. An overview studies examining interactions individual antioxidant compounds major blood is presented findings. Investigating antioxidant/protein at level organism determining distribution involvement in particular role very challenging task. However, by knowing protein certain pathology or aging, exerted bound it, it recommend intake resistance improve condition slow down process.

Language: Английский

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Astilbin inhibited neutrophil extracellular traps in gouty arthritis through suppression of purinergic P2Y6 receptor

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155754 - 155754

Published: May 17, 2024

Language: Английский

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Imaging pharmacological and edible behavior of Sinomenii Caulis volatile organic compounds: chain model insight

Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

As a traditional Chinese medicine, Sinomenii Caulis (SC) has not only attracted much attention for its medicinal value, but also volatile organic compounds have shown potential application in the agriculture and food fields. This study combined chain modeling (fingerprinting, machine learning, network pharmacology, metabolic analysis, spectroscopic experiments, physical simulations) with aim of interdisciplinarity exploring applications SC, at interface food, especially area additives active ingredient pharmacology. Palmitic acid (PA), linoleic (LA), isomenthone (IM), which are closely related to depression, were good biocompatibility stability, allowing them cross blood-brain barrier exert their therapeutic effects on depression. Through these ingredients can be effectively metabolized human body, thus ensuring safety food. spectral analysis simulation, it was verified that PA/LA/IM interact transporter proteins vivo transport. The SC used treatment depression as additives, fully transported perform roles, is beneficial development medicines flavored foods. provides feasible guide products pharmacological activities. © 2025 Society Chemical Industry.

Language: Английский

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How does HSA`s antioxidant activity change under the influence of lincomycin and spectinomycin mixture? – A spectroscopic study

Journal of Pharmaceutical and Biomedical Analysis Open, Journal Year: 2025, Volume and Issue: unknown, P. 100070 - 100070

Published: March 1, 2025

Language: Английский

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Lipase-catalyzed synthesis of astilbin fatty acid esters: effect of key reaction factors on catalysis and physicochemical properties of target products

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106416 - 106416

Published: March 1, 2025

Language: Английский

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Comparison on the conformation folding and structure change of serum albumin induced by methyl parathion and its metabolite p-nitrophenol

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106393 - 106393

Published: March 1, 2025

Language: Английский

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Detailed Experimental and In Silico Investigation of Indomethacin Binding with Human Serum Albumin Considering Primary and Secondary Binding Sites

Molecules, Journal Year: 2023, Volume and Issue: 28(7), P. 2979 - 2979

Published: March 27, 2023

The interaction of indomethacin with human serum albumin (HSA) has been studied here considering the primary and secondary binding sites. Stern-Volmer plots were linear in lower concentration range while a downward curvature was observed higher range, suggesting presence more than one site for inside HSA due to which microenvironment fluorophore changed slightly some its fraction not accessible quencher. quenching constants (KSV) sites calculated from two portions plots. There around two-fold decrease low-affinity as compared site. takes place via static mechanism KSV decreases at both upon increasing temperature. also evaluated, show strong fair thermodynamically favorable liberation heat ordering system. In principle, hydrogen bonding Van der Waals forces involved interacted through hydrophobic only. competitive evaluated using warfarin, ibuprofen, hemin, warfarin + hemin combination markers. profile remained unchanged whereas it decreased straight line reduction maximum when present simultaneously concentrations indomethacin. structure changes Molecular dynamics simulations performed are drug 1 (located subdomain IIA protein) IB), respectively. From results obtained molecular docking MD simulation, molecule showed affinity towards followed by other

Language: Английский

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The effect of selected aminoglycoside antibiotics on human serum albumin antioxidant activity: a spectroscopic and calorimetric comparative study

Pharmacological Reports, Journal Year: 2023, Volume and Issue: 75(5), P. 1276 - 1290

Published: Sept. 13, 2023

Abstract Background Human serum albumin (HSA) is a valuable component of non-enzymatic and endogenous antioxidant mechanisms. The activity HSA can be modulated by ligands, including drugs. Although this central topic in the field oxidation, there still lack information about protection against effects elevated free radical levels. Methods aim study was to investigate kanamycin (KAN) neomycin (NEO) their effect on potential using spectroscopic microcalorimetric techniques. Results Despite fact that interact with HSA, no changes secondary structure protein have been observed. analysis aminoglycoside antibiotics showed low synergistic interaction, probably due influence ligands (KAN, NEO) availability amino acid residues functional groups, such as thiol group (Cys-34). Conclusions Based data, both KAN NEO considered modulators activity.

Language: Английский

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Toxicological profile of the Hymenaea courbaril stem bark hydroalcoholic extract using in vitro bioassays and an alternative in vivo Caenorhabditis elegans model

Journal of Toxicology and Environmental Health, Journal Year: 2023, Volume and Issue: 86(18), P. 678 - 695

Published: July 24, 2023

ABSTRACTHymenaea genus has been used in folk medicine Brazil, but few studies investigated its toxicity profile. Thus, the aim of this study was to determine toxicological parameters Hymenaea courbaril stem bark hydroalcoholic extract by utilizing three cell lines including murine macrophages (RAW 264.7), mouse fibroblast cells (L929) and human lung (MRC-5), as well Salmonella/microsome assay, vivo Caenorhabditis elegans model. The predominant detected phytoconstituents were coumarins, flavonoids, phenolics, tannins saponins HPLC analysis, astilbin (AST) found be main component. DPPH assay demonstrated that H. exhibited potent antioxidant activity, with an IC50 3.12 μg/ml. at concentrations 400 800 μg/ml decreased viability 48 hr after treatment L929 MRC-5 lines. In Raw 264.7 strain, just highest concentration (800 μg/ml) lowered within following exposure. 100 did not markedly affect trypan blue assay. alkaline comet non-genotoxic. Ames test, low mutagenic potential without metabolic activation, since only produced effect. affected survival C. 1600 μl/ml. These findings demonstrate appears exert evidenced vitro mutagenicity assays; however, biological relevance response safety assessments needs further studies.KEYWORDS: AstilbinCaenorhabditis eleganscytotoxicitygenotoxicityhymenaea courbarilmutagenicity AcknowledgmentsThe author LACL thank Coordenação de Aperfeicoamento Pessoal Nivel Superior (CAPES) for doctoral scholarship.Disclosure statementNo conflict interest reported author(s).Credit authorship contribution statementLACL, MIG PP conceived designed experiments. FCS collected registered plant material. EME contributed reagents/materials/analysis tools.LACL, SEB VLB performed analysis.LACL mammalian LACL, PAS elegansstudies. JTS, JNP genotoxicity JNP, analyzed data. wrote paper. PP, critically revised manuscript.Data availability statementThe data support are available from corresponding (PP), upon reasonable request.Correction StatementThis article corrected minor changes. changes do impact academic content article.Additional informationFundingThis research supported [FinanceCode 001].

Language: Английский

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The mechanism of interaction between tri-para-cresyl phosphate and human serum protein: a multispectroscopic and in-silico study

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 400, P. 111144 - 111144

Published: July 14, 2024

Language: Английский

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