Oxidation Products of Tryptophan and Proline in Adipokinetic Hormones—Artifacts or Post-Translational Modifications? DOI Creative Commons

Simone König,

Heather G. Marco, Gerd GÄde

et al.

Life, Journal Year: 2023, Volume and Issue: 13(12), P. 2315 - 2315

Published: Dec. 10, 2023

Background: Adipokinetic hormones (AKHs) regulate important physiological processes in insects. AKHs are short peptides with blocked termini and Trp position 8. Often, proline occupies 6. Few post-translational modifications have been found, including hydroxyproline ([Hyp6]) kynurenine. Our recent data suggest that the Hyp- Kyn-containing occur more often than originally thought we here investigate if they natural or artifactual. Methods: From crude extracts of corpora cardiaca (CC) various insect species, were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-MS). Synthetic [Hyp6]-AKHs tested an vivo metabolic assay. Freshly dissected Periplaneta americana Blaberus atropos CCs (with precautions taken against oxidation) analyzed. B. CC placed into a depolarizing saline released measured. Results: Hyp was detected several decapeptides from cockroaches. The modified form accompanied AKH at concentrations below 7%. present fresh preparations shown be releasable ex vivo. [Hyp6]-containing positively hypertrehalosemic bioassay. Hydroxyprolination also for Manto-CC termite Kalotermes flavicollis Tetsu-AKH grasshopper, Tetrix subulata. Oxidized Trp-containing forms Nicve-AKH found species burying beetle genus Nicrophorus. Conclusions: oxidation is known easily during sample handling likely reason findings. For hydroxyprolination, however, experimental evidence suggests endogenous processes.

Language: Английский

The gonadotropin-releasing hormone (GnRH) superfamily across Pancrustacea/Tetraconata: A role in metabolism? DOI Creative Commons
Heather G. Marco, Susan Glendinning, Tomer Ventura

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 590, P. 112238 - 112238

Published: April 13, 2024

Language: Английский

Citations

4
Chemical ecology of triatomines: current knowledge and implications for Chagas disease vector management DOI Creative Commons
David Alavez‐Rosas,

Reyna Vargas‐Abasolo,

Claudia I. Albores-Flores

et al.

Journal of Pest Science, Journal Year: 2023, Volume and Issue: 97(2), P. 507 - 520

Published: Aug. 10, 2023

Abstract Triatomines (Hemiptera: Heteroptera: Reduviidae), commonly known as conenose or kissing bugs, includes species of outstanding medical importance given their role vectors the protozoan parasite Trypanosoma cruzi Chagas (Kinetoplastida: Trypanosomatidae), aetiological agent disease. Herein, we present an updated review chemical ecology triatomines, with special reference to molecules involved in communication these insects. Infochemicals, including hormones and semiochemicals (pheromones allelochemicals), have a pivotal mediating information shaping interactions within between during distinct behavioural contexts. We analyse new findings about composition alarm, aggregation sex pheromones, well on cuticular hydrocarbons, host attractants repellents. also discuss how triatomine may serve signals highlight reverse approach studying complex chemoecology arthropod vectors. In final section, outline knowledge can be used for implementing real-world control operations proven epidemiological impact.

Language: Английский

Citations

10
Comparative analysis of adipokinetic hormones and their receptors in Blattodea reveals novel patterns of gene evolution DOI Creative Commons
Shixiong Jiang, Heather G. Marco,

Nina Scheich

et al.

Insect Molecular Biology, Journal Year: 2023, Volume and Issue: 32(6), P. 615 - 633

Published: June 29, 2023

Adipokinetic hormone (AKH) is a neuropeptide produced in the insect corpora cardiaca that plays an essential role mobilising carbohydrates and lipids from fat body to haemolymph. AKH acts by binding rhodopsin-like G protein-coupled receptor (GPCR), adipokinetic (AKHR). In this study, we tackle ligand gene evolution as well evolutionary origins of paralogues order Blattodea (termites cockroaches). Phylogenetic analyses precursor sequences point ancient duplication event common ancestor Blaberoidea, yielding new group putative decapeptides. total, 16 different peptides 90 species were obtained. Two octapeptides seven putatively novel decapeptides are predicted for first time. 18 species, spanning solitary cockroaches subsocial wood roaches lower higher termites, subsequently acquired using classical molecular methods silico approaches employing transcriptomic data. Aligned AKHR open reading frames revealed 7 highly conserved transmembrane regions, typical arrangement GPCRs. based on support accepted relationships among termite, (Cryptocercus spp.) cockroach lineages large extent, while post-translational modification sites do not greatly differ between social termites. Our study provides important information only functional research but also further interested their development potential candidates biorational pest control agents against invasive termites cockroaches.

Language: Английский

Citations

7
The proline effect and the tryptophan immonium ion assist in de novo sequencing of adipokinetic hormones DOI Creative Commons

Simone König,

Heather G. Marco, Gerd GÄde

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 5, 2023

Adipokinetic hormones (AKHs) in Arthopoda are characterized by special sequence features including limited choices of amino acid residues certain positions, such as Trp position 8. Over 100 different AKHs have been described, but de novo sequencing novel peptide can be a challenge. In project analyzing corpora cardiaca extracts from two fly species, moths, termite and beetle for their AKHs, we noted specific patterns the fragmentation spectra octapeptides electrospray Q-TOF experiments resulting presence Pro 6. The preference cleavage N-terminal to created an abundant y3″-ion, which, conjunction with b-ions before after Pro, provided marker pattern. As Pro6 occurs about 61% known this information is highly relevant elucidation. Moreover, default Trp8 allowed use its immonium ion AKH candidate identification. addition, assembled sequences literature AKH-type format found Uniprot database single online resource. These efforts assisted analysis workflow led assignment evidence Melme-CC Dorpa-AKH corpus cardiacum scarab Sinodendron cylindricum.

Language: Английский

Citations

7
Predicted novel hypertrehalosaemic peptides of cockroaches are verified by mass spectrometry DOI Creative Commons
Heather G. Marco,

Simone König,

Gerd GÄde

et al.

Amino Acids, Journal Year: 2023, Volume and Issue: 55(11), P. 1641 - 1654

Published: Oct. 26, 2023

Small neuropeptides from the corpora cardiaca are responsible in cockroaches for mobilisation of trehalose fat body into haemolymph. Such hypertrehalosaemic hormones (HrTHs) belong to large family insect adipokinetic (AKHs); a few HrTHs were previously sequenced cockroaches, and genomic and/or transcriptomic information one may predict genes encoding more species. Definite elucidation primary structure mature peptide with putative modifications needs analytical chemical methods. In current study, we use high-resolution mass spectrometry coupled liquid chromatography identify unequivocally 13 cockroach Either genomic/transcriptomic was available most species examined, or related We confirm predicted novel sequences find hydroxyproline modification majority peptides. The decapeptides structurally close Bladi-HrTH, which is found all seven investigated blaberid subfamilies. Bladi-HrTH peptides elicit response Periplaneta americana, blattid cockroach.

Language: Английский

Citations

4
Molecular identification and the role of adipokinetic hormone receptor in Helicoverpa armigera (Hübner) DOI
Zhengkun Xiao, Ruoheng Jin, Yun Tan

et al.

Journal of Asia-Pacific Entomology, Journal Year: 2023, Volume and Issue: 26(2), P. 102070 - 102070

Published: March 15, 2023

Language: Английский

Citations

3
Oxidation Products of Tryptophan and Proline in Adipokinetic Hormones—Artifacts or Post-Translational Modifications? DOI Creative Commons

Simone König,

Heather G. Marco, Gerd GÄde

et al.

Life, Journal Year: 2023, Volume and Issue: 13(12), P. 2315 - 2315

Published: Dec. 10, 2023

Background: Adipokinetic hormones (AKHs) regulate important physiological processes in insects. AKHs are short peptides with blocked termini and Trp position 8. Often, proline occupies 6. Few post-translational modifications have been found, including hydroxyproline ([Hyp6]) kynurenine. Our recent data suggest that the Hyp- Kyn-containing occur more often than originally thought we here investigate if they natural or artifactual. Methods: From crude extracts of corpora cardiaca (CC) various insect species, were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-MS). Synthetic [Hyp6]-AKHs tested an vivo metabolic assay. Freshly dissected Periplaneta americana Blaberus atropos CCs (with precautions taken against oxidation) analyzed. B. CC placed into a depolarizing saline released measured. Results: Hyp was detected several decapeptides from cockroaches. The modified form accompanied AKH at concentrations below 7%. present fresh preparations shown be releasable ex vivo. [Hyp6]-containing positively hypertrehalosemic bioassay. Hydroxyprolination also for Manto-CC termite Kalotermes flavicollis Tetsu-AKH grasshopper, Tetrix subulata. Oxidized Trp-containing forms Nicve-AKH found species burying beetle genus Nicrophorus. Conclusions: oxidation is known easily during sample handling likely reason findings. For hydroxyprolination, however, experimental evidence suggests endogenous processes.

Language: Английский

Citations

2