Unveiling the potential of spirulina algal extract as promising antibacterial and antibiofilm agent against carbapenem-resistant Klebsiella pneumoniae: in vitro and in vivo study

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 5, 2025

Abstract Carbapenem-resistant Klebsiella pneumoniae poses a severe risk to global public health, necessitating the immediate development of novel therapeutic strategies. The current study aimed investigate effectiveness green algae Arthrospira maxima (commercially known as Spirulina ) both in vitro and vivo against carbapenem-resistant K. . In this study, thirty isolates were collected, identified, then screened for their susceptibility several antibiotics carbapenemase production genes using PCR. Both bla KPC OXA-48 most predominant detected tested isolates. phytochemical profiling A. algal extract was conducted LC–MS/MS positive mode technique. minimum inhibitory concentrations (MIC) ranged from 500 1000 µg/mL. also resulted decreasing membrane integrity distortion bacterial cells revealed by scanning electron microscope. bioactive compounds that responsible antibacterial action fatty acids, including PUFAs, polysaccharides, glycosides, peptides, flavonoids, phycocyanin, minerals, essential amino vitamins. Moreover, an antibiofilm activity crystal violet assay qRT-PCR. A murine pneumonia model employed assessment extract. showed promising which comparable colistin (standard drug). This manifested improving pulmonary architecture, inflammatory cell infiltration, fibrosis after staining with hematoxylin eosin Masson’s trichrome stain. Using immunohistochemical investigations, percentage immunoreactive significantly decreased monoclonal antibodies tumor necrosis factor-alpha interleukin six. So, may be considered new candidate medications. Graphical

Language: Английский

---

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2100 - 2100

Published: Feb. 8, 2024

The rising prevalence of drug-resistant bacteria underscores the need to search for innovative and nature-based solutions. One approaches may be use plants that constitute a rich source miscellaneous compounds with wide range biological properties. This review explores antimicrobial activity seven bioactives their possible molecular mechanisms action. Special attention was focused on antibacterial properties berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, sanguinarine against Staphylococcus aureus, Enterococcus spp., Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Serratia marcescens Pseudomonas aeruginosa. growing interest in novel therapeutic strategies based new plant-derived formulations confirmed by number articles. Natural products are one most promising intensively examined agents combat consequences overuse misuse classical antibiotics.

Language: Английский

---

Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 2, 2024

Pseudomonas aeruginosa biofilm is a community of bacteria that adhere to live or non-living surfaces and are encapsulated by an extracellular polymeric substance. Unlike individual planktonic cells, biofilms possess notable inherent resistance sanitizers antibiotics. Overcoming this substantial barrier in the medical food industries. Hence, while antibiotics ineffective eradicating P. biofilm, scientists have explored alternate strategies, including utilization natural compounds as novel treatment option. To end, curcumin, carvacrol, thymol, eugenol, cinnamaldehyde, coumarin, catechin, terpinene-4-ol, linalool, pinene, linoleic acid, saponin, geraniol major extensively utilized for management community. Noteworthy, exact interaction bacterium not elucidated yet; however, interference with quorum sensing system inhibition autoinducer production main possible mechanisms. use different drug platforms can overcome some drawbacks compounds, such insolubility water, limited oral bioavailability, fast metabolism, degradation. Additionally, deliver antibiofilm agents simultaneously, which enhances potential compounds. This article explores many facets utilizing inhibit eradicate biofilms. It also examines techniques protocols employed enhance effectiveness these

Language: Английский

---

Critical review on plant-derived quorum sensing signaling inhibitors in pseudomonas aeruginosa

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 151, P. 107649 - 107649

Published: July 15, 2024

Language: Английский

---

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 17, 2024

Abstract Background Biofilms and oxidative stress retard wound healing. The resistance of biofilms to antibiotics has led a search for alternative approaches in biofilm elimination. Antioxidants work synergistically with antibacterial agents against biofilms. Hence recent research suggested plants as candidates the development new alternatives treatments antioxidants due presence phytocompounds which are responsible their bioactivities. Hoslundi opposita Vahl is one used by traditional healers treat wounds other infections, this makes it potential candidate drug discovery hence, study, we investigate antibiofilm antioxidant activity methanolic extract hoslundia V ahl from Uganda. We also identify phytochemicals its bioactivity. Method plant was extracted maceration using methanol, investigated 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. microtiter plate assay (MTP) where Minimum inhibitory concentration required inhibit 50% or 90% (MBIC 50 MBIC 90 ) eradication remove (MBEC MBEC were measured. It further analysed phytochemical composition quantitative screening, well Gas chromatography-mass spectrometry (GC-MS) Liquid chromatography mass-spectrometry (LC-MS). Results H. Opposita showed good 249.6 mg/mL. inhibited growth P. aeruginosa S. aureus 28.37 mg/mL 10 mg/mL, respectively. ability eradicate 23.85 39.01 Phytochemical analysis revealed alkaloids, tannins, flavonoids, phenols. GC-MS 122 compounds which, 23 have evidence literature. most abundant were; 1,4- Citric acid, Tetracontane-1,40-diol (43.43.3%, 1, Olean-12-en-28-oic 3-hydroxy-, methyl ester, (3.beta) (15.36%) 9-Octadecenamide (12.50%), Squalene (11.85%) Palmitic Acid 4TMS (11.28%), alpha Amyrin (11.27%). LC-MS identified 115 57 multiple reaction mode (MRM) scan modes Conclusion bioactive identified, hence study justifies use can be utilised drugs antioxidants.

Language: Английский

---

Potential Use of Selected Natural Compounds with Anti-Biofilm Activity

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 607 - 607

Published: Jan. 13, 2025

Antibiotic resistance in microorganisms is an escalating global concern, exacerbated by their formation of biofilms, which provide protection through extracellular matrix and communication via quorum sensing, enhancing to treatment. This situation has driven the search for alternative approaches, particularly those using natural compounds. study explores potential phytochemicals, such as quercetin, apigenin, arbutin, gallic acid, proanthocyanidins, rutin, known antibacterial properties ability inhibit biofilm disrupt mature biofilms. The methods used this included a comprehensive review current literature assessing bioavailability, distribution, effective concentrations these compounds treating biofilm-associated infections. results indicate that phytochemicals exhibit significant effects, reduce biofilm’s structural integrity, bacterial pathways. Moreover, use combination with existing antibiotics may enhance therapeutic outcomes. findings support conclusion offer promising additions anti-biofilm strategies are capable complementing or replacing conventional treatments, appropriate levels delivery mechanisms being key effectiveness. insight underscores need further research into clinical applications infections complicated

Language: Английский

---

Exploring daidzein dimethyl ether from Albizzia lebbeck as a novel quorum sensing inhibitor against Pseudomonas aeruginosa: Insights from in vitro and in vivo studies

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 156, P. 108168 - 108168

Published: Jan. 16, 2025

Language: Английский

---

Phenolic Compounds from Pyrus communis Residues: Mechanisms of Antibacterial Action and Therapeutic Applications

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 280 - 280

Published: March 8, 2025

Background/Objectives: The food industry produces substantial amounts of fruit byproducts, which are often discarded despite their high content bioactive compounds with potential therapeutic applications. Pyrus communis (pear) residues, particularly rich in phenolic compounds, represent a valuable yet underutilized resource. These byproducts have demonstrated significant antioxidant and antibacterial properties, suggesting for medical pharmaceutical This review aims to provide comprehensive analysis the profile P. emphasizing mechanisms prospective use combating oxidative stress resistance. Methods: A key from residues was conducted using ScienceDirect Google Scholar databases (from 2014 2024). Studies assessing activities were reviewed, focus on action against Gram-positive Gram-negative bacterial pathogens. Results: minimum 14 distinct identified among residues. However, chlorogenic acid catechin as primary contributors activity Hydroquinone exhibited strong effects through membrane disruption, enzyme inhibition, metabolic interference. Despite this potential, hydroquinone’s cytotoxicity regulatory concerns limit its direct application. Conclusions: While phenolics show promise natural agents, future research should address bioavailability, extraction standardization, safe formulation strategies. Investigating synergy conventional antibiotics improving stability cosmetic applications steps toward practical use. In vivo clinical studies crucial validating ensuring approval.

Language: Английский

---

Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: March 18, 2025

Options to combat bacterial infections are becoming scarce. We require innovative approaches enhance the discovery of effective antimicrobials capable combating bacteria resistant multiple or all antibiotics. These methods should either directly eliminate indirectly influence their viability by inhibiting virulence reducing resistance One interesting approach is analyze ancient remedies used treat infections, formulate them, and test them against modern microbes. This field has recently been named "ancientbiotics." allows us leverage centuries empirical knowledge accumulated, from traditional medicines across various cultures worldwide. The strategy already yielded promising formulations ESKAPE group nosocomial pathogens. Additionally, molecular de-extinction, which involves genome analysis extinct species search for useful antimicrobials, such as peptides, offers another avenue. In this review, we compile antimicrobial effects de-extinct molecules known science discuss possible new strategies further harness potential past fight rise superbugs.

Language: Английский

---

Unveiling the potential antibacterial action of acetylcysteine for managing Staphylococcus aureus wound infections: in vitro and in vivo study

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: April 1, 2025

Language: Английский

---