Cited by Additional file 1 of Bufalin targeting CAMKK2 inhibits the occurrence and development of intrahepatic cholangiocarcinoma through Wnt/β-catenin signal pathway

Bufalin CaCO₃ Nanoparticles Triggered Pyroptosis through Calcium Overload via Na⁺/Ca²⁺ Exchanger Reverse for Cancer Immunotherapy DOI

Tingxuan Li,

Yitong Zhang,

Hanyue Li

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Bufalin is a promising active ingredient in traditional Chinese medicine but has shown limited anticancer applications due to its toxicity. Here, we report BCNPs@gel, bufalin-containing CaCO

Language: Английский

Citations

Pharmacological insights and role of bufalin (bufadienolides) in inflammation modulation: a narrative review DOI

Gagandeep Kaur, Sushma Devi, Akhil Sharma

et al.

Inflammopharmacology, Journal Year: 2024, Volume and Issue: 32(5), P. 3057 - 3077

Published: July 16, 2024

Language: Английский

Citations

Bufalin targeting CAMKK2 inhibits the occurrence and development of intrahepatic cholangiocarcinoma through Wnt/β-catenin signal pathway DOI

Huhu Zhang, Xiaolei Dong, Xiaoyan Ding

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: Dec. 11, 2023

Abstract Background Intrahepatic cholangiocarcinoma (ICC) accounts for about 15% of primary liver cancer, and the incidence rate has been rising in recent years. Surgical resection is best treatment ICC, but 5-year survival less than 30%. ICC signature genes are crucial early diagnosis so it especially important to find its therapeutic drug. Here, we studied that bufalin targeting CAMKK2 promotes mitochondrial dysfunction inhibits occurrence metastasis intrahepatic through Wnt/β-catenin signal pathway. Methods IC50 cells was determined by CCK8 invasive migratory abilities were verified wound healing, cell cloning, transwell Western blot. IF IHC expression between patients normal subjects. BLI pull-down demonstrated binding ability CAMKK2. Bioinformatics predicted whether related SKL2001, an activator β-catenin, acted this In vitro vivo experiments overexpression affects proliferative effects ICC. Transmission electron microscopy integrity. Associated Ca 2+ levels biological ANXA2 on Results It found inhibited proliferation migration highly expressed high positively correlated with poor prognosis.CAMKK2 a direct target bufalin, associated signaling pathway, which dose-dependently decreased after treatment. promoted migration, reversed effect. , changes content induced protein ANXA2, decreasing cytoplasmic increasing overexpressing cells, knocked down, reversal overexpression-induced enhancement siANXA2. Conclusions Our results suggest Thus, as drug, may also be used cancer therapy future.

Language: Английский

Citations

Global trends in bufalin application research for cancer from 2003 to 2022: A bibliometric and visualised analysis DOI

Donghao Tang,

Yuejiao Feng,

Jiahao Lu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(2), P. e24395 - e24395

Published: Jan. 1, 2024

BackgroundBufalin, the main active ingredient of traditional Chinese medicine huachansu, is used in clinical treatment colorectal cancer and has multiple effects, including inhibition migratory invasion, reversal multi-drug resistance, induction apoptosis differentiation, angiogenesis.MethodsWe collected relevant articles on bufalin from 2003 to 2022 using Web Science platform, analysed information VOSviewer, CiteSpace, Microsoft Excel categorise summarise publications over past 20 years.ResultsWe 371 papers, with a steady increase number published globally. China highest articles, whereas Japan citations. Currently, there considerable enthusiasm for investigating anti-tumour mechanism optimising drug delivery systems its administration.ConclusionFor first time, we present comprehensive overview papers worldwide two decades progress application tumour therapy. We summarised key authors, institutions, countries that have contributed field potential cancer. This will help other researchers obtain an field, enhance collaboration knowledge sharing, promote future research bufalin.

Language: Английский

Citations

Mechanisms mediating effects of cardiotonic steroids in mammalian blood cells DOI

Yuri M. Poluektov, O. D. Lopina, Maria A. Strelkova

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Cardiotonic steroids (CTSs) were known as steroidal plant compounds that exert cellular effects by the binding to Na,K-ATPase. Earlier, (exogenous) CTSs used treat chronic heart failure. By now, endogenous CTS have been identified in mammals, and their concentrations blood, normally a subnanomolar range, are altered numerous pathologies. This indicates role regulators of physiological processes. transport occurs primarily yet on blood cells remain poorly understood. review summarizes animals humans under normal pathological conditions, analyzes action based mechanisms mammalian cells. At high (greater than 10 −9 M), Na,K-ATPase inhibits enzyme, whereas lower induce signaling cascades or activate enzyme. All these shown be present The particular effect is determined type, its concentration, isoform composition catalytic α-subunit cell, other cell features. It has demonstrated all types (erythrocytes, leukocytes, platelets) expressed both ubiquitously distributed α1-isoform tissue-specific α3-subunit, which exhibits different ion affinity compared α1. results wide spectrum responses fluctuations levels blood. In particular, an increase level more twofold sufficient decline activity erythrocyte administration exogenous able modulate proinflammatory attributed activation cascades, influence platelet activation. Hence, alterations bloodstream significantly affect functionality cells, contributing organism’s adaptive response. On top this, comparison human leukocytes rodent carrying CTS-resistant often reveals opposite effects, thus indicating rodents unsuitable model for studying

Language: Английский

Citations

ATP1A1 is a promising new target for melanoma treatment and can be inhibited by its physiological ligand bufalin to restore targeted therapy efficacy DOI

Laura Soumoy,

Aline Genbauffe,

Lena Mouchart

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 4, 2024

Abstract Despite advancements in treating metastatic melanoma, many patients exhibit resistance to targeted therapies. Our study focuses on ATP1A1, a sodium pump subunit associated with cancer development. We aimed assess ATP1A1 prognostic value melanoma and examine the impact of its ligand, bufalin, cell lines vitro vivo. High expression (IHC) correlated reduced overall survival patients. Resistance BRAF inhibitor was linked elevated levels patient biopsies (IHC, qPCR) (Western blot, qPCR). Additionally, high mRNA positively differentiation/pigmentation markers based data from The Cancer Genome Atlas (TCGA) databases Verfaillie proliferative gene signature analysis. Bufalin specifically caveolae, (proximity ligation assay) influenced Src phosphorylation blot), thereby disrupting multiple signaling pathways (phosphokinase array). In vitro, bufalin induced apoptosis by acting (siRNA experiments) and, vivo, significantly impeded growth using nude mouse xenograft model continuous delivery via an osmotic pump. conclusion, our demonstrates that could serve as marker for predictive response therapy. By targeting inhibited proliferation, effectively suppressed tumor development mice. Thus, findings strongly support promising therapeutic target, potential agent disrupt tumor-promoting activity.

Language: Английский

Citations

Natural products combating EGFR-TKIs resistance in cancer DOI

Xia Li,

Gaohui Zhu,

Qiyao Peng

et al.

European Journal of Medicinal Chemistry Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100251 - 100251

Published: Feb. 1, 2025

Language: Английский

Citations

Deciphering the Enigmatic Influence: Non-Coding RNAs Orchestrating Wnt/β-Catenin Signaling Pathway in Tumor Progression DOI

Xinbing Yang,

Yajing Du,

Lulu Luo

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 13909 - 13909

Published: Sept. 10, 2023

Dysregulated expression of specific non-coding RNAs (ncRNAs) has been strongly linked to tumorigenesis, cancer progression, and therapeutic resistance. These ncRNAs can act as either oncogenes or tumor suppressors, thereby serving valuable diagnostic prognostic markers. Numerous studies have implicated the participation in regulation diverse signaling pathways, including pivotal Wnt/β-catenin pathway that is widely acknowledged for its role embryogenesis, cellular proliferation, biology control. Recent emerging evidence shed light on capacity interact with key components pathway, modulating Wnt target genes cells. Notably, activity this reciprocally influence levels ncRNAs. However, comprehensive analysis investigating associated their intricate interactions remains elusive. Based these noteworthy findings, review aims unravel associations between during initiation, potential implications interventions. Additionally, we provide a overview characteristics accompanied by thorough discussion functional roles biology. Targeting molecules may emerge promising effective strategy future treatments.

Language: Английский

Citations

Regulatory role of circular RNAs in oral squamous cell carcinoma DOI

Rükset Attar, Khalida I. Noel, Mirna Azalea Romero

et al.

Cellular and Molecular Biology, Journal Year: 2023, Volume and Issue: 69(8), P. 250 - 257

Published: Aug. 31, 2023

OSCC is a genomically complicated disease and advancements in the modern era of molecular oncology have enabled researchers to portray near-to-complete resolution signaling landscape. Over last two decades, overwhelming proof-of-concept has established mechanistic regulatory role non-coding RNAs carcinogenesis, including OSCC. Circular demonstrate burgeoning facet research biologists are only beginning appreciate recognize significance circRNAs pathogenesis Regulatory roles re-shaping pathways offer plausible strategies for prevention/inhibition "sponge effects" mediated by wider variety need be rationally targeted effective cancer prevention. Phenomenal cutting-edge works different types animal models will further refine our knowledge selection most promising as pharmacologically valuable targets.

Language: Английский

Citations

Bufalin Suppresses Head and Neck Cancer Development by Modulating Immune Responses and Targeting the β-Catenin Signaling Pathway DOI

Nour Mhaidly,

Noura Barake,

Anne Trelcat

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(15), P. 2739 - 2739

Published: Aug. 1, 2024

Bufalin, a cardiotonic steroid derived from the Chinese toad (Bufo gargarizans), has demonstrated potent anticancer properties across various cancer types, positioning it as promising therapeutic candidate. However, comprehensive mechanistic studies specific to head and neck cancers have been lacking. Our study aimed bridge this gap by investigating bufalin’s mechanisms of action in cells. Using several methods, such Western blotting, immunofluorescence, flow cytometry, we observed dose-dependent reduction cell viability, disruption membrane integrity, inhibition colony formation both HPV-positive HPV-negative lines. Bufalin induces apoptosis through modulation apoptosis-related proteins, mitochondrial function, reactive oxygen species production. It also arrests cycle at G2/M phase attenuates migration while affecting epithelial–mesenchymal transition markers targeting pivotal signaling pathways, including Wnt/β-catenin, EGFR, NF-κB. Additionally, bufalin exerted immunomodulatory effects polarizing macrophages toward M1 phenotype, bolstering antitumor immune responses. These findings underscore potential multifaceted agent against cancers, essential pathways involved proliferation, apoptosis, regulation, metastasis, modulation. Further research is warranted validate these optimize clinical application.

Language: Английский

Citations