PA-Win2: In Silico-Based Discovery of a Novel Peptide with Dual Antibacterial and Anti-Biofilm Activity DOI Creative Commons

Jin Wook Oh,

Min Kyoung Shin,

Hye-Ran Park

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1113 - 1113

Published: Nov. 21, 2024

Background: The emergence and prevalence of antibiotic-resistant bacteria (ARBs) have become a serious global threat, as the morbidity mortality associated with ARB infections are continuously rising. activation quorum sensing (QS) genes can promote biofilm formation, which contributes to acquisition drug resistance increases virulence. Therefore, there is an urgent need develop new antimicrobial agents control prevent further development. Antimicrobial peptides (AMPs) naturally occurring defense molecules in organisms known suppress pathogens through broad range mechanisms. Methods: In this study, we utilized previously developed deep-learning model identify AMP candidates from venom gland transcriptome spider Pardosa astrigera, followed by experimental validation. Results: PA-Win2 was among top-scoring predicted selected based on physiochemical features. Subsequent validation demonstrated that inhibits growth Bacillus subtilis, Escherichia coli, Staphylococcus aureus, epidermidis, Pseudomonas aeruginosa, multidrug-resistant P. aeruginosa (MRPA) strain CCARM 2095. peptide exhibited strong bactericidal activity against MRPA 2095 depolarization bacterial cytoplasmic membranes alteration gene expression survival. addition, effectively inhibited formation degraded pre-formed biofilms aeruginosa. study showed treatment led downregulation QS Las, Pqs, Rhl systems. Conclusions: These findings suggest promising candidate demonstrate potential silico methods discovering functional biological data.

Language: Английский

Venoms and Extracellular Vesicles: A New Frontier in Venom Biology DOI Creative Commons
Auwal A. Bala,

Naoual Oukkache,

Elda E. Sánchez

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(1), P. 36 - 36

Published: Jan. 14, 2025

Extracellular vesicles (EVs) are nanoparticle-sized secreted by nearly all cell types under normal physiological conditions. In toxicological research, EVs have emerged as a crucial link between public health and multi-omics approaches, offering insights into cellular responses to disease-causing injury agents such environmental biological toxins, contaminants, drugs. Notably, present unique opportunity deepen our understanding of the pathophysiology envenomation natural toxins. Recent advancements in isolating purifying EV cargo, mass spectrometry techniques, bioinformatics positioned potential biomarkers that could elucidate signaling pathways provide valuable information on relationship venomous their mechanisms action, effectiveness antivenoms. Additionally, hold promise proxies for various aspects envenomation, including toxin dosage, characterization, progression, prognosis during therapeutic interventions. These can be explored through technology applied contents from plasma, saliva, or urine samples envenomated individuals, comprehensive integrative approach managing cases.

Language: Английский

Citations

1
Analgesic Peptides: From Natural Diversity to Rational Design DOI Creative Commons
Katarzyna Gach, Monika Biernat, Mariola Kuczer

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(7), P. 1544 - 1544

Published: March 29, 2024

Pain affects one-third of the global population and is a significant public health issue. The use opioid drugs, which are strongest painkillers, associated with several side effects, such as tolerance, addiction, overdose, even death. An increasing demand for novel, safer analgesic agents driving force exploring natural sources bioactive peptides antinociceptive activity. Since G protein-coupled receptors (GPCRs) play crucial role in pain modulation, discovery new peptide ligands GPCRs challenge novel drug development. aim this review to present human animal origin potential show possibilities their modification, well design structures. study presents current knowledge on structure-activity relationship peptide-based biomimetic compounds, modification strategies directed at activity, improvement metabolic stability pharmacodynamic profile. procedures employed prolonged delivery emerging compounds also discussed. work summarizes conditions leading development morphine replacements.

Language: Английский

Citations

5
Molluscan and Arthropod Neurotoxins DOI
Rocio K. Finol‐Urdaneta

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Artificial diet for the paddy wolf spiders Pardosa pseudoannulata: Survival, Development, and Implications for mass rearing DOI
Lelei Wen, Haixin Zhang, Zhilin Zhang

et al.

Journal of Asia-Pacific Entomology, Journal Year: 2025, Volume and Issue: unknown, P. 102418 - 102418

Published: April 1, 2025

Language: Английский

Citations

0
Invertebrate venoms: A treasure trove of bioactive compounds with anticancer potential DOI
Ayoub Lafnoune, Asmaa Chbel, Bouchra Darkaoui

et al.

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
"Acidic and Basic Proteins from Spider Latrodectus Pallidus Venom that Induce Formation of Non-Bilayer Phase and Increase Proton Capacity in Model Myelin Membranes Feature High Sequence Homology to Isoforms of Myelin Basic Protein: Pharmacological Relevance" DOI Open Access
Edward S. Gasanoff

Biomedical Journal of Scientific & Technical Research, Journal Year: 2024, Volume and Issue: 56(4)

Published: May 23, 2024

This study describes the isolation of novel acidic and basic proteins from venom Latrodectus pallidus (white widow spider), which exhibit high amino acid sequence homology to 18.5 kDa isoforms myelin proteins. explores ability model membranes, composed phospholipids spider venom, absorb protons form a non-bilayer lipid phase.

Language: Английский

Citations

3
Zilucoplan: A Newly Approved Macrocyclic Peptide for Treatment of Anti-Acetylcholine Receptor Positive Myasthenia Gravis DOI Creative Commons
Lia Costa, Carla Fernandes

Drugs and Drug Candidates, Journal Year: 2024, Volume and Issue: 3(2), P. 311 - 327

Published: March 27, 2024

Zilucoplan is a synthetic macrocyclic peptide approved by the Food and Drug Administration (FDA), in October 2023, for treatment of generalized myasthenia gravis. It considered as an orphan drug that causes inhibition terminal complement cascade activation with dual mechanism action preventing formation membrane attack complex (MAC) destruction neuromuscular junction. This has been demonstrated to be able treat gravis without significant adverse effects, good efficacy, safety, tolerability profile. not only innovative promising therapeutics gravis, but it could also beneficial other diseases well model synthesis analogues improve pharmacological

Language: Английский

Citations

2
Identification and Evolutionary Analysis of the Widely Distributed CAP Superfamily in Spider Venom DOI Creative Commons

Hongcen Jiang,

Yiru A. Wang, Guoqing Zhang

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(6), P. 240 - 240

Published: May 24, 2024

Venom plays a crucial role in the defense and predation of venomous animals. Spiders (Araneae) are among most successful predators have fascinating venom composition. Their mainly contains disulfide-rich peptides large proteins. Here, we analyzed spider protein families, utilizing transcriptomic genomic data, highlighted their similarities differences. We show that spiders specific combinations toxins for better defense, typically comprising core toxin expressed alongside several auxiliary toxins. Among them, CAP superfamily is widely distributed highly web-building Araneoidea spiders. Our analysis evolutionary relationships revealed four subfamilies (subA-subD) differ structure potential functions. proteins composed conserved domain diverse C-terminal domains. subC shares similar domains with snake ion channel regulator svCRISP proteins, while subD possesses sequence to insect allergen 5 (Ag5). Furthermore, gene duplication selective expression lead increased subD, making it member superfamily. This study sheds light on functional diversity history, which has important implications fully understanding composition components

Language: Английский

Citations

1
Primary cell culture from embryos of the common house spider Parasteatoda tepidariorum DOI Creative Commons

Afrah Hassan,

Giulia Zancolli

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Background Spiders have emerged as valuable models in evolutionary developmental biology, but primary cell cultures from spider embryonic tissues not been fully explored. In this study, we describe the first successful long-term cultivation of cells common house spider, Parasteatoda tepidariorum. We initiated five independent using mechanical and enzymatic dissociation methods, comparing two culture media, Leibovitz’s L-15 Grace’s Insect Medium, under varying pH conditions. Results Cultures exhibited diverse morphologies, including round suspension elongated, neuron-like cells. The most culture, with medium at 7, was passaged four times maintained for over six months. also tested collagen type I-coated wells to improve adhesion. Our results indicate that P. tepidariorum proliferate better supports growth, while promotes more differentiation. Conclusions This system provides a platform functional genomics studies, potential applications biology research.

Language: Английский

Citations

0
PA-Win2: In Silico-Based Discovery of a Novel Peptide with Dual Antibacterial and Anti-Biofilm Activity DOI Creative Commons

Jin Wook Oh,

Min Kyoung Shin,

Hye-Ran Park

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1113 - 1113

Published: Nov. 21, 2024

Background: The emergence and prevalence of antibiotic-resistant bacteria (ARBs) have become a serious global threat, as the morbidity mortality associated with ARB infections are continuously rising. activation quorum sensing (QS) genes can promote biofilm formation, which contributes to acquisition drug resistance increases virulence. Therefore, there is an urgent need develop new antimicrobial agents control prevent further development. Antimicrobial peptides (AMPs) naturally occurring defense molecules in organisms known suppress pathogens through broad range mechanisms. Methods: In this study, we utilized previously developed deep-learning model identify AMP candidates from venom gland transcriptome spider Pardosa astrigera, followed by experimental validation. Results: PA-Win2 was among top-scoring predicted selected based on physiochemical features. Subsequent validation demonstrated that inhibits growth Bacillus subtilis, Escherichia coli, Staphylococcus aureus, epidermidis, Pseudomonas aeruginosa, multidrug-resistant P. aeruginosa (MRPA) strain CCARM 2095. peptide exhibited strong bactericidal activity against MRPA 2095 depolarization bacterial cytoplasmic membranes alteration gene expression survival. addition, effectively inhibited formation degraded pre-formed biofilms aeruginosa. study showed treatment led downregulation QS Las, Pqs, Rhl systems. Conclusions: These findings suggest promising candidate demonstrate potential silico methods discovering functional biological data.

Language: Английский

Citations

0