Total Sanghuangporus vaninii extract inhibits hepatocyte ferroptosis and intestinal microbiota disturbance to attenuate liver fibrosis in mice

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119571 - 119571

Published: Feb. 1, 2025

Language: Английский

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A Comprehensive Review of the Diversity of Fungal Secondary Metabolites and Their Emerging Applications in Healthcare and Environment

Mycobiology, Journal Year: 2024, Volume and Issue: 52(6), P. 335 - 387

Published: Nov. 1, 2024

Fungi and their natural products, like secondary metabolites, have gained a huge demand in the last decade due to increasing applications healthcare, environmental cleanup, biotechnology-based industries. The fungi produce these metabolites (SMs) during different phases of growth, which are categorized into terpenoids, alkaloids, polyketides, non-ribosomal peptides. These SMs exhibit significant biological activity, contributes formulation novel pharmaceuticals, biopesticides, bioremediation agents. Nowadays, fungal-derived widely used food beverages, for fermentation, preservatives, protein sources, dairy In it is being as an antimicrobial, anticancer, anti-inflammatory, immunosuppressive drug. usage modern tools biotechnology can achieve increase large-scale production. present review comprehensively analyses diversity fungal along with emerging agriculture, sustainability, nutraceuticals. Here, authors reviewed recent advancements genetic engineering, metabolic pathway manipulation, synthetic biology improve production yield SMs. Advancement fermentation techniques, bioprocessing, co-cultivation approaches Investigators further highlighted importance omics technologies understanding regulation biosynthesis SMs, offers drug discovery sustainable agriculture. Finally, addressed potential manipulation biotechnological innovations exploitation commercial benefits.

Language: Английский

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UPLC-MS metabolite profiling and antioxidant activity of Sanghuangporus sanghuang extract

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18758 - e18758

Published: Jan. 22, 2025

Background The objective of the present study is to examine total phenolic and flavonoid content an ethanol extract Sanghuangporus sanghuang evaluate its phytochemical properties, antioxidant activity, capacity protect DNA from damage. This pharmaceutical/food resource mushroom may serve as a novel substitute functional food for health-conscious consumers, given promising source phenolics flavonoids. Methods S. (SEE) was evaluated contents, while UPLC-MS analysis used terpenoids, phenylpropanoid, flavonoids, steroidal, phenols identification, function prediction. Antioxidant anti-DNA damage activities were tested in vitro using ferric reducing power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS), protection assay. Results Conclusion Total (TPC) SEE 385.38 ± 1.36 mg GA/g extract, (TFC) 298.22 2.38 QE/g extract. extracts exhibited high free radical scavenging with relatively stronger activity. A 491 metabolites investigated by Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Most top 20 compounds predicted have various functions like antioxidant, anti-cancer anti-inflammatory. highlighted beneficial It contains potential natural that could be lead contender development medicines treatment wide range oxidative stress-related illnesses.

Language: Английский

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Bioactivity profiling of Sanghuangporus lonicerinus : antioxidant, hypoglycaemic, and anticancer potential via in-vitro and in-silico approaches

Journal of Enzyme Inhibition and Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 40(1)

Published: Feb. 24, 2025

This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl3), hot water (H2O) extracts Sanghuangporus lonicerinus from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, FRAP assays, in vitro hypoglycaemic effects were evaluated through α-amylase α-glucosidase inhibition. Antiproliferative potential explored by analysing binding affinities EtOH H2O to estrogen receptor α (ERα), ERβ, androgen (AR), glucocorticoid (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, extract containing highest total content (143.15 ± 6.70 mg GAE/g d.w.) best antioxidant capacity. The showed significant effects, 85.29 5.58% inhibition 41.21 0.79% α-amylase. Moderate ERβ suggests for estrogen-mediated cancer therapy, while strong AKR1C3 supports its therapeutic potential.

Language: Английский

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Multiomics reveals the molecular mechanism of unsaturated fatty acid-induced terpenoid biosynthesis in Sanghuangporus lonicericola

npj Science of Food, Journal Year: 2025, Volume and Issue: 9(1)

Published: March 26, 2025

Treatment with C18:1 and C18:2, but not C18:0, increased the triterpenoid content of medicinal fungus Sanghuangporus lonicericola. We identified 413 terpenoids, including 210 volatile terpenoids. Eight upregulated 3,13,15-trihydroxyoleanane-12-one, dulcioic acid serrat-14-ene-3,20,24,29-tetrol, were shared between C18:2 treatments C18:0 treatment. The levels 12 7 odour-related respectively, level alpha-farnesene (herbal odour). Gene set enrichment analysis revealed that compared produced stronger activation terpenoid biosynthesis, fatty degradation, MAPK signalling pathways inhibition basal transcription factors at both transcript protein levels. Finally, two-way orthogonal partial least squares gene expression in was correlated unsaturated acid-induced metabolites. Together, our integrated multiomics data key involved biosynthesis S.

Language: Английский

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Paclobutrazol induces triterpenoid biosynthesis via downregulation of the negative transcriptional regulator SlMYB in Sanghuangporus lonicericola

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 3, 2025

Triterpenoids are well-known pharmacological components of Sanghuangporus fungi, such as lonicericola. This study investigates the inductive effects paclobutrazol (PBZ) on triterpenoid biosynthesis in submerged fermentation S. lonicericola and explores induction mechanisms via multi-omics genetic methods. The addition 100 mg/L PBZ significantly increases yield by 151.39%. A total 29 triterpenoids tentatively identified, which 18 newly presented only under induction. Moreover, 30 genes involved MVA pathway 31 encoding cytochrome P450 monooxygenases assumed to be responsible for decoration identified. Finally, a MYB transcription factor (SlMYB) is identified found downregulated Genetic manipulation SlMYB demonstrates its negative regulatory effect four putative target genes, including ACAT, MVD, IDI, FDPS. Electrophoretic mobility gel shift assays verify direct interactions with promoters Taken together, acts an effective inducer lonicericola, negatively regulated.

Language: Английский

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A conserved terpene cyclase gene in Sanghuangporus for abscisic acid-related sesquiterpenoid biosynthesis

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 15, 2025

Abstract Background The medicinal mushroom Sanghuangporus is renowned in East Asia for its potent therapeutic properties, attributed part to bioactive sesquiterpenoids. However, despite their recognized potential, the biosynthetic pathways and specific enzymes responsible sesquiterpenoid production remain unexplored, limiting opportunities optimize applications. Results Sesquiterpenoids from four species were extracted through targeted isolation using mass spectrometry (MS)-based metabolomics, resulting discovery of six known abscisic acid-related compounds one new compound, whose structure was determined spectroscopic computational analysis. We employed a natural product genome mining approach identify putative gene cluster (BGC) containing sesquiterpene synthase gene, ancA , associated with detected compounds. Biosynthetic these proposed based on an integrative combining BGC analysis MS2 fragment-based dereplication. Further analyses revealed that content synteny are relatively well-conserved across but less so outside genus. Conclusions A BGC, pathway group sesquiterpenoids predicted genomic metabolic data analyses. This study addresses critical gap understanding genetic basis offers insights future research engineering enhance use.

Language: Английский

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Exploring the Wound Healing Potential of Hispidin

Nutrients, Journal Year: 2024, Volume and Issue: 16(18), P. 3161 - 3161

Published: Sept. 19, 2024

Background: Hispidin, a polyphenol component mainly derived from the medicinal mushroom species Phellinus and Inonotus, shows promise for biomedical applications, yet its potential in wound healing remains largely unexplored. This research investigates effects of hispidin through vitro vivo experiments, while also evaluating antimicrobial properties safety profile. Methods: In scratch assays were conducted to evaluate impact on migration NIH-3T3 cells. The was assessed rats using excision wounds, dead space linear incisions, treated with various topical ointments including simple ointment, 2.5% (w/w) 5% 0.2% nitrofurazone administered at 0.2 g daily 14 days. Results: Hispidin demonstrated particularly effective against Staphylococcus epidermidis. enhanced cell viability, promoted closure assays, correlating increased levels FGF21, TGF-β1, EGF, VEGF. models, ointment improved contraction, epithelialization, tissue regeneration, fibroblast activity, angiogenesis. granulation reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) lipid peroxidation, increasing anti-inflammatory (IL-10) antioxidant activities (SOD, GPx, CAT), along connective markers like hydroxyproline, hexosamine, hexuronic acid. breaking strength incision models. Acute dermal toxicity studies indicated no adverse 2000 mg/kg. Conclusions: These findings highlight hispidin’s care, demonstrating antimicrobial, regenerative, properties.

Language: Английский

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Tandem Mass Spectrometry Imaging to Assess Illudin S Distributions in Omphalotus Illudens Mushrooms

Published: Jan. 1, 2024

Poisoning metabolites within mushrooms typically exert varying physiological and biological effects; however, their distributions are seldom investigated. This study focused on imaging illudin S, a representative poisoning metabolite Omphalotus illudens mushroom, using desorption electrospray ionization–tandem mass spectrometry (DESI–MS/MS) coupled with liquid chromatography–electrospray (LC–ESI–MS/MS). Initially, LC–ESI–MS/MS conditions were established for the selective detection of S (m/z 247→159, positive mode) from extracted mushroom. Based this MS/MS condition, each mushroom was divided into nine sections, section subjected to specific DESI–MS/MS mode (pixel size: 200 × μm). The results revealed high concentrations in stalks growing trees. Further, re-evaluations LC–MS/MS assay seven-fold difference between stalk other regions. marks first attempt at assessing toxic poisonous mushrooms, thus offering valuable insights including efficient utilization analogs applications practices mitigating food risks.

Language: Английский

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Tandem mass spectrometry imaging to assess illudin S distributions in Omphalotus illudens mushrooms

Talanta Open, Journal Year: 2024, Volume and Issue: 10, P. 100335 - 100335

Published: June 4, 2024

Poisoning metabolites within mushrooms typically exert varying physiological and biological effects; however, their distributions are seldom investigated. This study focused on imaging illudin S, a representative poisoning metabolite Omphalotus illudens mushroom, using desorption electrospray ionization–tandem mass spectrometry (DESI–MS/MS) coupled with liquid chromatography–electrospray (LC–ESI–MS/MS). Initially, LC–ESI–MS/MS conditions were established for the selective detection of S (m/z 247→159, positive mode) from extracted mushroom. Based this MS/MS condition, each mushroom was divided into nine sections, section subjected to specific DESI–MS/MS mode (pixel size: 200 × μm). The results revealed high concentrations in stalks growing trees. Further, re-evaluations LC–MS/MS assay seven-fold difference between stalk other regions. marks first attempt at assessing toxic poisonous mushrooms, thus offering valuable insights including efficient utilization analogs applications.

Language: Английский

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