Preliminary Study on Lutetium-177 and Gold Nanoparticles: Apoptosis and Radiation Enhancement in Hepatic Cancer Cell Line DOI Creative Commons
Maria Anthi Kouri,

A. P. Georgopoulos,

G. Manios

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(11), P. 12244 - 12259

Published: Oct. 30, 2024

This study investigates a novel approach toward enhancing the efficacy of Lutetium-177 (Lu-177) radiopharmaceutical therapy by combining it with gold nanoparticles (AuNPs) in HepG2 hepatic cancer cell line. Lu-177, known for its effective β radiation, also emits gamma rays at energies (113 keV and 208 keV) near photoelectric absorption range, suggesting potential targeted localized radiation enhancement when used conjunction AuNPs. Thus, cells were treated two different activity levels (74 MBq 148 MBq), Lu-177 alone, combination AuNPs sizes (10 nm 50 nm), while some received no treatment. Treatment was assessed quantifying ratio (RER) apoptosis levels. The results reveal that significantly increases death compared to 10 demonstrating superior effectiveness. Additionally, varying influenced treatment outcomes, higher further augmenting therapeutic impact combined therapy. These findings underscore utilizing Lu-177's beta, but gamma, emissions, traditionally considered non-therapeutic, strategy leverages as an internal irradiator exploit advantage without requiring nanoparticle functionalization. provides promising improving radionuclide sets stage future research aimed optimizing treatments through use

Language: Английский

Наночастицы серебра и золота, синтезированные с помощью β-циклодекстрина, как радиосенсибилизаторы при лучевой терапии рака молочной железы DOI Creative Commons
Маргарита Пустовалова,

Виктор Некрасов,

Е. В. Андреев

et al.

Published: Jan. 19, 2025

Цели: 1) Синтез и характеризация наночастиц серебра золота, стабилизированных β-циклодекстрином; 2) Оценка влияния синтезированных на выраженность радиобиологических эффектов в облученных клетках рака молочной железы (РМЖ). Материалы методы: Наночастицы золота синтезировали с использованием β-циклодекстрина качестве восстановителя стабилизатора. В работе были использованы клеточные линии РМЖ человека MDA-MB-231 (ER-/PR-/ EGFR+/ HER2-) MCF7 (ER+/ PR+/ EGFR-/ HER2-). Клетки облучали рентгеновской биологической установке РУБ РУСТ-М1 (Россия), оснащенной двумя рентгеновскими излучателями, при мощности дозы 40 мГр/мин, напряжении 100 кВ, токе 0.8 мА, фильтре 1.5 мм Al. вносили за 24 ч до облучения концентрации 0,5 мг/л. без использовались контроля. Для оценки анализировали фокусы белка-маркера повреждений ДНК (γН2АХ) через 1 после облучения. Статистический математический анализ данных проводили программного обеспечения GraphPad Prism 9.0.2.161 (GraphPad Software). Статистическую значимость оценивали дисперсионного анализа (ANOVA). Результаты: Показана эффективная радиосенсибилизация клеток (РМЖ) линий помощью серебра, основе β-циклодекстрина. Полученные результаты свидетельствуют о достижении статистически значимых результатов уже мг/л, что как минимум 20 раз меньше концентраций, используемых ранее для достижения значимого эффекта.

Language: Русский

Citations

0
Silver nanoparticles enhance neutron radiation sensitivity in cancer cells: An in vitro study DOI Creative Commons
Evgenii Plotnikov, A. G. Drozd,

A.A. Artamonov

et al.

Nanomedicine Nanotechnology Biology and Medicine, Journal Year: 2025, Volume and Issue: 65, P. 102813 - 102813

Published: Feb. 28, 2025

Growing interest in cancer radiotherapy has led to the application of nanoparticles as radiosensitizers. Here, we, for first time, present results radiosensitizing properties silver (AgNPs) (possessing low toxicity towards human body) against cells under neutron irradiation. Five standard cultures (including glioblastoma, known its resistance conventional photon radiation) were used evaluate AgNPs suing MTT test, flow cytometry, and optical fluorescence microscopy. Neutron irradiation was applied absorbed dose 0.5-1.5 Gy with an average energy 7.5 MeV. increased efficiency radiosensitivity enhancement ratios 1.02-2.32, glioblastoma 1.22-1.47. It revealed that at 1.5 Gy, AgNP-induced cytotoxicity made a significant contribution total observed radiosensitizer effect: on average, five cell types, 29.8 96.2 % AgNP concentration 0.2 1.6 μg/mL, respectively.

Language: Английский

Citations

0
Chemical mechanism in gold nanoparticles radiosensitization: A Monte Carlo simulation study DOI Creative Commons
Joana Antunes, Hans Rabus, Filipa Mendes

et al.

Radiation Physics and Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 112637 - 112637

Published: March 1, 2025

Language: Английский

Citations

0
Dose comparison of slowing-down protons and secondary electrons by gold nanoparticle in proton therapy: simulation study DOI

Farshid Tabbakh

The European Physical Journal Plus, Journal Year: 2025, Volume and Issue: 140(4)

Published: April 1, 2025

Language: Английский

Citations

0
Nanoparticles with “K-edge” Metals Bring “Color” in Multiscale Spectral Photon Counting X-ray Imaging DOI
Nivetha Gunaseelan, Pranay Saha,

Nada Maher

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Preclinical and clinical diagnostics depend greatly on medical imaging, which enables the identification of physiological pathological processes in living subjects. It is often necessary to use contrast agents complement anatomical data with functional information or describe disease phenotypically. Nanomaterials are used as many advanced bioimaging techniques applications because their high payload, physicochemical properties, improved sensitivity, multimodality. Metals k-edge energy within X-ray bandwidth respond photon counting spectral imaging. This Perspective examines progress made emerging area nanoparticle-based agents. These nano

Language: Английский

Citations

1
Preliminary Study on Lutetium-177 and Gold Nanoparticles: Apoptosis and Radiation Enhancement in Hepatic Cancer Cell Line DOI Creative Commons
Maria Anthi Kouri,

A. P. Georgopoulos,

G. Manios

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(11), P. 12244 - 12259

Published: Oct. 30, 2024

This study investigates a novel approach toward enhancing the efficacy of Lutetium-177 (Lu-177) radiopharmaceutical therapy by combining it with gold nanoparticles (AuNPs) in HepG2 hepatic cancer cell line. Lu-177, known for its effective β radiation, also emits gamma rays at energies (113 keV and 208 keV) near photoelectric absorption range, suggesting potential targeted localized radiation enhancement when used conjunction AuNPs. Thus, cells were treated two different activity levels (74 MBq 148 MBq), Lu-177 alone, combination AuNPs sizes (10 nm 50 nm), while some received no treatment. Treatment was assessed quantifying ratio (RER) apoptosis levels. The results reveal that significantly increases death compared to 10 demonstrating superior effectiveness. Additionally, varying influenced treatment outcomes, higher further augmenting therapeutic impact combined therapy. These findings underscore utilizing Lu-177's beta, but gamma, emissions, traditionally considered non-therapeutic, strategy leverages as an internal irradiator exploit advantage without requiring nanoparticle functionalization. provides promising improving radionuclide sets stage future research aimed optimizing treatments through use

Language: Английский

Citations

0