Shifts in sulphur-cycling bacteria in the rhizobiome support the adaptation of Caulerpa prolifera to elevated sulphide levels DOI Creative Commons

Anastasiia Barilo,

Aschwin H. Engelen, Susanne Wilken

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 24, 2024

Caulerpa is a genus of green macroalgae that lives in tropical and subtropical coastal waters. It an intriguing organism because, despite having plant-like structures, it one giant cell – which, next to multiple nuclei, chloroplasts, mitochondria, also contains endo- epiphytic bacteria. The role these bacteria unknown, but they might impact the growth development host, adaptation environmental parameters, and, hence, ecological success algae. We hypothesised increased sulphide concentrations would trigger significant shift microbial community composition associated with C. prolifera rhizoids, favouring sulphide-oxidizing To test this hypothesis, we conducted mesocosm experiment incubating sediments different analysed algal photosynthesis, growth, microbiome composition. While photosynthesis was not affected, weight-based rate decreased linearly increasing concentration. analyse microbiome, extracted DNA RNA from fronds, accompanying performed 16S amplicon sequencing. fronds unaffected both samples. However, increase concentration coincided decrease relative abundance sulphate-reducing particularly family Desulfocapsaceae. In samples, potential oxidisers rhizoid-associated members Beggiatoaceae were detected. Our results suggest rhizobiome plays its sulphide-rich environments, offering new insights into complex interactions within marine holobionts.

Language: Английский

Microbial diagnostics in periodontal diseases DOI
Daniel Manoil, Ana Parga, Nagihan Bostancı

et al.

Periodontology 2000, Journal Year: 2024, Volume and Issue: 95(1), P. 176 - 193

Published: May 26, 2024

Microbial analytical methods have been instrumental in elucidating the complex microbial etiology of periodontal diseases, by shaping our understanding subgingival community dynamics. Certain pathobionts can orchestrate establishment dysbiotic communities that subvert host immune system, triggering inflammation and tissue destruction. Yet, diagnosis management conditions still rely on clinical radiographic examinations, overlooking well-established etiology. This review summarizes chronological emergence etiological models co-evolution with technological advances detection. We additionally approaches currently accessible to clinicians, highlighting their value broadening assessment. The epidemiological importance obtaining culture-based antimicrobial susceptibility profiles taxa for antibiotic resistance surveillance is also underscored, together clinically relevant guide antibiotherapy choices, when necessary. Furthermore, 16S-based shotgun metagenomic profiling discussed outlining signatures. Because dysbiosis precedes damage, biomarker identification offers early diagnostic possibilities forestall disease relapses during maintenance. Altogether, this highlights underutilized potential microbiology periodontology, spotlighting areas most conductive its implementation enhancing prevention, treatment predictability, addressing global resistance.

Language: Английский

Citations

16
Comparison of the full-length sequence and sub-regions of 16S rRNA gene for skin microbiome profiling DOI Creative Commons
Han Zhang, Xiang Wang, An‐Qi Chen

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(7)

Published: June 27, 2024

The skin microbiome plays a pivotal role in human health by providing protective and functional benefits. Furthermore, its inherent stability individual specificity present novel forensic applications. These aspects have sparked considerable research enthusiasm among scholars across various fields. However, the selection of specific 16S rRNA hypervariable regions for studies is not standardized should be validated through extensive tailored to different objectives targeted bacterial taxa. Notably, third-generation sequencing (TGS) technology leverages full discriminatory power gene enables more detailed accurate microbial community analyses. Here, we conducted full-length 141 microbiota samples from multiple anatomical sites using PacBio platform. Based on this data, generated derived sub-region data an

Language: Английский

Citations

5
Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence DOI

Nor Husna Mat Hussin,

Hajar Fauzan Ahmad

AIP conference proceedings, Journal Year: 2025, Volume and Issue: 3275, P. 060002 - 060002

Published: Jan. 1, 2025

Language: Английский

Citations

0
Culturable Human Microorganisms and the Impact of Transportation Conditions on Cultivability DOI Creative Commons

Xibei Fan,

Ning Lv,

Zhe‐Xue Quan

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 549 - 549

Published: Feb. 28, 2025

The composition of the human microbiome is a critical health indicator, and culture-independent methodologies have substantially advanced our understanding human-associated microorganisms. However, precise identification characterization microbial strains require culture-based techniques. Recently, resurgence culturomics, combined with high-throughput sequencing technology, has reduced high labor demand pure culture methods, facilitating more efficient comprehensive acquisition culturable strains. This study employed an integrated approach combining culturomic to identify microorganisms on scalp in saliva feces. Several Staphylococcus were identified from scalp, whereas anaerobic dominant fecal samples. Additionally, highlighted beneficial effects transportation conditions (liquid nitrogen treatment, dry ice transport, dimethyl sulfoxide [DMSO] buffer) preserving A robust methodology was developed for large-scale optimized transport that enhance potential isolating greater diversity

Language: Английский

Citations

0
Oral microbiome changes in subjects with obesity following bariatric surgery compared to lean counterparts DOI Creative Commons
Keun‐Suh Kim, Hee Sam Na, Tae Jung Oh

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

This study aimed to compare oral microbiome profiles between obese and lean individuals without clinical periodontitis, assess changes in the of subjects following bariatric surgery. Individuals with a body mass index (BMI) > 30 were enrolled group, whereas those BMI < 23 served as controls. The surgery which consented surgery, was followed up at 1, 3, 6 months examinations. Oral examinations conducted periodontal disease classified based on probing results. Saliva, buccal subgingival samples analyzed for community diversity, relative bacterial abundance, differential abundance control (n = 24) group 31). To evaluate effect size statistical power, we used micropower, simulation-based method Permutational Multivariate Analysis Variance-based β-diversity comparisons. exhibited distinct alpha diversity (buccal: Chao1 p 0.0002, Shannon 0.0003, supragingival: 0.0001) compared group. Bray-Curtis distance analysis indicated significant disparities composition distribution saliva (p 0.003), 0.002), plaque 0.001). Although normal weight groups no differences, showed species associated disease, especially including Filifactor alocis, Peptostreptococcaceae spp., Prevotella Treponema maltophilum. Cluster emergence microbiomes healthy state that increased over time Streptococcus salivarious various Veillonella clusters containing pathogens Porphyromonas tended diminish. post-bariatric indicates potential shift toward state, suggesting loss interventions may positively impact microbial communities even absence periodontitis.

Language: Английский

Citations

0
How raw milk-based adjunct cultures influence microbial diversity in cheese DOI
Matthias Dreier,

Luca Bettera,

Hélène Berthoud

et al.

International Dairy Journal, Journal Year: 2025, Volume and Issue: unknown, P. 106249 - 106249

Published: March 1, 2025

Language: Английский

Citations

0
Microbiological profile of molars with advanced furcation defects before and after surgical and non-surgical periodontal treatment: A pilot randomized controlled trial DOI
Pasquale Santamaria, Priya Bahal, Mandeep Ghuman

et al.

Journal of Dentistry, Journal Year: 2025, Volume and Issue: 157, P. 105746 - 105746

Published: April 18, 2025

Language: Английский

Citations

0
Next-Generation Sequencing in Periprosthetic Joint Infections DOI Creative Commons

Ravina Kullar,

Craig D. Tipton, Thomas M. File

et al.

Infectious Diseases in Clinical Practice, Journal Year: 2025, Volume and Issue: 33(3)

Published: March 14, 2025

Abstract Periprosthetic joint infection (PJI) is 1 of the most dreaded complications following total hip arthroplasty and knee arthroplasty. Early diagnosis optimal treatment are critical for managing PJI, accurate detection causative pathogens may be aided by recently available nucleic acid–based diagnostics. Next-generation sequencing (NGS) has demonstrated particular value in culture-negative PJI cases atypical organisms. The use NGS can provide substantial benefits to antimicrobial stewardship treatment. facilitates earlier more effective interventions patients enabling rapid precise identification pathogens. This technology especially crucial detecting rare, novel, or polymicrobial infections, which traditional diagnostic methods often fail identify.

Language: Английский

Citations

0
From wolves to humans: oral microbiome resistance to transfer across mammalian hosts DOI Creative Commons
Nicholas A. Podar, Alyssa A. Carrell, Kira A. Cassidy

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(3)

Published: Feb. 1, 2024

ABSTRACT The mammalian mouth is colonized by complex microbial communities, adapted to specific niches, and in homeostasis with the host. Individual microbes interact metabolically rely primarily on nutrients provided host, which they have potentially co-evolved along lineages. oral environment similar across mammals, but diversity, specificity, evolution of community structure related or interacting mammals are little understood. Here, we compared microbiomes dogs those wild wolves humans. In dogs, found an increased diversity relative wolves, possibly transition omnivorous nutrition following domestication. This includes a larger Patescibacteria than previously reported any other microbiota. most distinct at bacterial species strain levels, few if shared between humans canids, while close evolutionary relationship reflected numerous taxa. More taxa higher taxonomic levels including humans, supporting their more ancestral common colonization followed diversification. Phylogenies selected lineages do not support stable human-dog transfers suggest diversification (apes canids). Therefore, despite millennia cohabitation interaction, host its native controls limits assimilation new microbes, even closely related. Higher resolution metagenomic physiological studies, covering should help understand how communities assemble, adapt, hosts. IMPORTANCE Numerous types colonize after birth play important roles maintaining health. When microbiota-host perturbed, proliferation some bacteria leads diseases such as caries periodontitis. Unlike gut microbiome, space Our study apes (humans, chimpanzees, bonobos), aim identifying been exchanged result domestication cohabitation. We evidence for exchanges. significance our research finding that microbiota and/or limit acquisition exogenous context natural exclusion potential novel pathogens. provide framework expanded higher-resolution studies domestic animals resistance/resilience.

Language: Английский

Citations

1
Aqueous ecosystem: Environmental metagenomics, water quality, and possible remediation measures DOI
Inderjeet Tyagi, Kaomud Tyagi, Faheem Ahamad

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Jan. 1, 2024

Language: Английский

Citations

1