Non-Coding RNAs as Key Regulators of Glutaminolysis in Cancer DOI Open Access
Yunuen Ortiz-Pedraza, J. Omar Muñoz-Bello, Leslie Olmedo‐Nieva

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2872 - 2872

Published: April 20, 2020

Cancer cells exhibit exacerbated metabolic activity to maintain their accelerated proliferation and microenvironmental adaptation in order survive under nutrient-deficient conditions. Tumors display an increase glycolysis, glutaminolysis fatty acid biosynthesis, which provide energy source. Glutamine is critical for fundamental cellular processes, where intermediate metabolites produced through are necessary the maintenance of mitochondrial metabolism. These include antioxidants remove reactive oxygen species, generation nonessential amino acids, purines, pyrimidines acids required replication activation cell signaling. Some cancer highly dependent on glutamine consumption since its catabolism provides anaplerotic pathway feed Krebs cycle. Intermediate members have been found be deregulated several types cancers proposed as therapeutic targets prognostic biomarkers. This review summarizes main players pathway, how they implications maintenance. Furthermore, non-coding RNAs now recognized new participants regulation glutaminolysis; therefore, involvement metabolism discussed detail.

Language: Английский

Long non-coding RNAs: definitions, functions, challenges and recommendations DOI Open Access
John S. Mattick, Paulo Amaral, Piero Carninci

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(6), P. 430 - 447

Published: Jan. 3, 2023

Language: Английский

Citations

1207
PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications DOI
Junchao Shi, Yunfang Zhang, Dongmei Tan

et al.

Nature Cell Biology, Journal Year: 2021, Volume and Issue: 23(4), P. 424 - 436

Published: April 1, 2021

Language: Английский

Citations

186
tRNA renovatio: Rebirth through fragmentation DOI Creative Commons
Bernhard Kuhle, Qi Chen, Paul Schimmel

et al.

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(22), P. 3953 - 3971

Published: Oct. 5, 2023

tRNA function is based on unique structures that enable mRNA decoding using anticodon trinucleotides. These interact with specific aminoacyl-tRNA synthetases and ribosomes 3D shape sequence signatures. Beyond translation, tRNAs serve as versatile signaling molecules interacting other RNAs proteins. Through evolutionary processes, fragmentation emerges not merely random degradation but an act of recreation, generating shorter called tRNA-derived small (tsRNAs). tsRNAs exploit their linear sequences newly arranged for unexpected biological functions, epitomizing the "renovatio" (from Latin, meaning renewal, renovation, rebirth). Emerging methods to uncover full tRNA/tsRNA modifications, combined techniques study RNA integrate AI-powered predictions, will comprehensive investigations products new interaction potentials in relation functions. We anticipate these directions herald a era understanding complexity advancing pharmaceutical engineering.

Language: Английский

Citations

45
Biomarker Detection in Early Diagnosis of Cancer: Recent Achievements in Point-of-Care Devices Based on Paper Microfluidics DOI Creative Commons
Bilge Asci Erkocyigit, Ozge Ozufuklar, Ayşenur Yardım

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 387 - 387

Published: March 15, 2023

Microfluidics is very crucial in lab-on-a-chip systems for carrying out operations a large-scale laboratory environment on single chip. Microfluidic are miniaturized devices which the fluid behavior and control can be manipulated small platform, with surface forces platform being greater than volumetric depending test method used. In recent years, paper-based microfluidic analytical (μPADs) have been developed to used point-of-care (POC) technologies. μPADs numerous advantages, including ease of use, low cost, capillary action liquid transfer without need power, ability store reagents active form fiber network, capability perform multiple tests using various measurement techniques. These benefits critical advancement microfluidics fields disease diagnosis, drug application, food safety. Cancer one most diseases early detection all around world. Detecting cancer-specific biomarkers provides significant data both diagnosis controlling progression. cancer biomarker hold great promise improving cure rates, quality life, minimizing treatment costs. Although types bioanalytical platforms available biomarkers, there limited studies reviews literature. Hence, this article aims draw attention these gaps literature as well features that future should have.

Language: Английский

Citations

44
Paternal Contributions to Offspring Health: Role of Sperm Small RNAs in Intergenerational Transmission of Epigenetic Information DOI Creative Commons
Upasna Sharma

Frontiers in Cell and Developmental Biology, Journal Year: 2019, Volume and Issue: 7

Published: Oct. 9, 2019

The most fundamental process for the perpetuation of a species is transfer information from parent to offspring. Although genomic DNA contributes majority inheritance, it now clear that epigenetic —information beyond underlying sequence— also passed on future generations. However, mechanism and extent such inheritance are not well understood. Here, I review some concepts, evidence, mechanisms intergenerational via sperm small RNAs. Recent studies provide evidence mature highly abundant in non-coding These RNAs modulated by paternal environmental conditions potentially delivered zygote at fertilization, where they can regulate early embryonic development. Intriguingly, RNA payload undergoes dramatic changes during testicular post-testicular maturation, making epigenome unique distinct germ cells. explore remodeling maturation epididymis, potential role this reprogramming inheritance.

Language: Английский

Citations

129
Prevalence of small base-pairing RNAs derived from diverse genomic loci DOI
Philip P. Adams, Gisela Storz

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Journal Year: 2020, Volume and Issue: 1863(7), P. 194524 - 194524

Published: March 9, 2020

Language: Английский

Citations

80
Principles and innovative technologies for decrypting noncoding RNAs: from discovery and functional prediction to clinical application DOI Creative Commons
Yu-Meng Sun, Yujie Chen

Journal of Hematology & Oncology, Journal Year: 2020, Volume and Issue: 13(1)

Published: Aug. 10, 2020

Abstract Noncoding RNAs (ncRNAs) are a large segment of the transcriptome that do not have apparent protein-coding roles, but they been verified to play important roles in diverse biological processes, including disease pathogenesis. With development innovative technologies, an increasing number novel ncRNAs uncovered; information about their prominent tissue-specific expression patterns, various interaction networks, and subcellular locations will undoubtedly enhance our understanding potential functions. Here, we summarized principles methods for identifications functional cancer biology. Moreover, this review also provides alternative ncRNA databases based on high-throughput sequencing or experimental validation, it briefly describes current strategy clinical translation cancer-associated be used diagnosis.

Language: Английский

Citations

80
Measurements Methods for the Development of MicroRNA-Based Tests for Cancer Diagnosis DOI Open Access
Francesca Precazzini, Simone Detassis, Andrea Imperatori

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(3), P. 1176 - 1176

Published: Jan. 25, 2021

Studies investigating microRNAs as potential biomarkers for cancer, immune-related diseases, or cardiac pathogenic among others, have exponentially increased in the last years. In particular, altered expression of specific miRNAs correlates with occurrence several making these molecules molecular tools non-invasive diagnosis, prognosis, and response to therapy. Nonetheless, are not clinical use yet, due inconsistencies literature regarding identified a disease, which turn can be attributed reasons, including lack assay standardization reproducibility. Technological limitations circulating measurement been, date, biggest challenge using settings. this review we will discuss pre-analytical, analytical, post-analytical challenges address technical biases patient-related parameters that an influence should improved translate miRNA stage. Moreover, describe currently available methods profiling measurement, underlining their advantages pitfalls.

Language: Английский

Citations

61
TACOS: a novel approach for accurate prediction of cell-specific long noncoding RNAs subcellular localization DOI Creative Commons
Young-Jun Jeon, Md Mehedi Hasan, Hyun Woo Park

et al.

Briefings in Bioinformatics, Journal Year: 2022, Volume and Issue: 23(4)

Published: June 26, 2022

Long noncoding RNAs (lncRNAs) are primarily regulated by their cellular localization, which is responsible for molecular functions, including cell cycle regulation and genome rearrangements. Accurately identifying the subcellular location of lncRNAs from sequence information crucial a better understanding biological functions mechanisms. In contrast to traditional experimental methods, bioinformatics or computational methods can be applied annotation lncRNA locations in humans more effectively. past, several machine learning-based have been developed identify but relevant work cell-specific localization human remains limited. this study, we present first application tree-based stacking approach, TACOS, allows users 10 different types. Specifically, conducted comprehensive evaluations six classifiers with feature descriptors, using newly constructed balanced training dataset each type. Subsequently, strengths AdaBoost baseline models were integrated via an appropriate classifier final prediction. TACOS displayed consistent performance both cross-validation independent assessments compared other two approaches employed study. The user-friendly online web server accessed at https://balalab-skku.org/TACOS.

Language: Английский

Citations

42
Transgenerational transmission of reproductive and metabolic dysfunction in the male progeny of polycystic ovary syndrome DOI Creative Commons
Sanjiv Risal, Congru Li, Qing Luo

et al.

Cell Reports Medicine, Journal Year: 2023, Volume and Issue: 4(5), P. 101035 - 101035

Published: May 1, 2023

The transgenerational maternal effects of polycystic ovary syndrome (PCOS) in female progeny are being revealed. As there is evidence that a male equivalent PCOS may exists, we ask whether sons born to mothers with (PCOS-sons) transmit reproductive and metabolic phenotypes their progeny. Here, register-based cohort clinical case-control study, find PCOS-sons more often obese dyslipidemic. Our prenatal androgenized PCOS-like mouse model or without diet-induced obesity confirmed dysfunctions first-generation (F1) offspring passed down F3. Sequencing F1-F3 sperm reveals distinct differentially expressed (DE) small non-coding RNAs (sncRNAs) across generations each lineage. Notably, common targets between DEsncRNAs serum indicate similar hyperandrogenism, strengthening the translational relevance highlighting previously underappreciated risk transmission dysfunction via germline.

Language: Английский

Citations

27