Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenserbaerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H2O2-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(5), P. 325 - 325

Published: May 14, 2022

For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (Acipenser baerii) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds proteases. The collagen hydrolysate (SCH) generated Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) hydroxide (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) isolated from SCH, they identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, RGEPGL with molecular weights 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, 627.70 Da, respectively. IELFPGLP highest activity on DPPH· (EC50: 1.27, 1.05, 1.38 mg/mL, respectively) HO· 1.16, 0.97, 1.63 respectively), inhibiting capability lipid peroxidation, protective functions H2O2-damaged plasmid DNA. More importantly, displayed significant cytoprotection HUVECs against H2O2 injury regulating endogenous enzymes superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) decrease contents reactive oxygen species (ROS) malondialdehyde (MDA). Therefore, research provided better technical assistance for a higher-value utilization peptides-especially IELFPGLP-which may serve additives generating health-prone products treat chronic diseases caused oxidative stress.

Language: Английский

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Antioxidant Role of Kaempferol in Prevention of Hepatocellular Carcinoma

Antioxidants, Journal Year: 2021, Volume and Issue: 10(9), P. 1419 - 1419

Published: Sept. 5, 2021

Reactive oxygen species (ROS) are noxious to cells because their increased level interacts with the body’s defense mechanism. These also cause mutations and uncontrolled cell division, resulting in oxidative stress (OS). Prolonged is responsible for incorrect protein folding endoplasmic reticulum (ER), causing a stressful condition, ER stress. cellular stresses (oxidative stress) well-recognized biological factors that play prominent role progression of hepatocellular carcinoma (HCC). HCC critical global health problem third leading cancer-related mortality. The application anti-oxidants from herbal sources significantly reduces Kaempferol (KP) naturally occurring, aglycone dietary flavonoid present various plants (Crocus sativus, Coccinia grandis, Euphorbia pekinensis, varieties Aloe vera, etc.) It capable interacting pleiotropic proteins human body. Efforts progress develop KP as potential candidate prevent no adverse effects. This review emphasizes molecular mechanism treating HCC, targeting

Language: Английский

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The Role of Oxidative Stress in NAFLD–NASH–HCC Transition—Focus on NADPH Oxidases

Biomedicines, Journal Year: 2021, Volume and Issue: 9(6), P. 687 - 687

Published: June 17, 2021

A peculiar role for oxidative stress in non-alcoholic fatty liver disease (NAFLD) and its transition to the inflammatory complication steatohepatitis (NASH), as well threatening evolution hepatocellular carcinoma (HCC), is supported by numerous experimental clinical studies. NADPH oxidases (NOXs) are enzymes producing reactive oxygen species (ROS), whose abundance cells closely related inflammation immune responses. Here, we reviewed recent findings regarding this topic, focusing on of NOXs different stages describing current knowledge about their mechanisms action. We conclude that, although there a consensus that NOX-produced ROS toxic non-neoplastic conditions due vicious cycle sustaining NAFLD NASH, effect controversial neoplastic towards HCC. In regard, indications differential NOX isoforms, since NOX1 NOX2 play detrimental role, whereas increased NOX4 expression appears be correlated with better HCC prognosis some Further studies needed fully unravel action relationships signaling pathways modulating steatosis cancer development.

Language: Английский

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Liver Inflammation and Hepatobiliary Cancers

Trends in cancer, Journal Year: 2021, Volume and Issue: 7(7), P. 606 - 623

Published: March 2, 2021

Language: Английский

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Oxidative Stress in Non-Alcoholic Fatty Liver Disease

Livers, Journal Year: 2022, Volume and Issue: 2(1), P. 30 - 76

Published: Feb. 1, 2022

Non-alcoholic fatty liver disease (NAFLD) is a challenging caused by multiple factors, which may partly explain why it still remains an orphan of adequate therapies. This review highlights the interaction between oxidative stress (OS) and disturbed lipid metabolism. Several reactive oxygen species generators, including those produced in gastrointestinal tract, contribute to lipotoxic hepatic (and extrahepatic) damage acids great variety their biologically active metabolites “multiple parallel-hit model”. leads inflammation fibrogenesis contributes NAFLD progression. The alterations oxidant/antioxidant balance affect also metabolism-related organelles, leading peroxidation, mitochondrial dysfunction, endoplasmic reticulum stress. OS-induced at least partially counteracted physiological antioxidant response. Therefore, modulation this defense system emerges as interesting target prevent development For instance, probiotics, prebiotics, diet, fecal microbiota transplantation represent new therapeutic approaches targeting gut dysbiosis. OS its counter-regulation are under influence individual genetic epigenetic factors well. In near future, precision medicine taking into consideration or environmental risk coupled with biomarkers, will likely assist noninvasive diagnosis monitoring progression further personalizing treatments.

Language: Английский

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The Roles of NFR2-Regulated Oxidative Stress and Mitochondrial Quality Control in Chronic Liver Diseases

Antioxidants, Journal Year: 2023, Volume and Issue: 12(11), P. 1928 - 1928

Published: Oct. 29, 2023

Chronic liver disease (CLD) affects a significant portion of the global population, leading to substantial number deaths each year. Distinct forms like non-alcoholic fatty (NAFLD) and alcoholic (ALD), though they have different etiologies, highlight shared pathologies rooted in oxidative stress. Central metabolism, mitochondria are essential for ATP production, gluconeogenesis, acid oxidation, heme synthesis. However, diseases NAFLD, ALD, fibrosis, mitochondrial function is compromised by inflammatory cytokines, hepatotoxins, metabolic irregularities. This dysfunction, especially electron leakage, exacerbates production reactive oxygen species (ROS), augmenting damage. Amidst this, nuclear factor erythroid 2-related 2 (NRF2) emerges as cellular protector. It not only counters stress regulating antioxidant genes but also maintains health overseeing autophagy biogenesis. The synergy between NRF2 modulation introduces new therapeutic potentials CLD, focusing on preserving integrity against threats. review delves into intricate role shedding light innovative strategies its prevention treatment, through pathways.

Language: Английский

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Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Nov. 15, 2021

As a natural active substance, dihydromyricetin (DHM) has been proven to have good hepatoprotective activity. However, the therapeutic effect of DHM on liver fibrosis, which become disease threatening health people around world, not studied date. The purpose this study was investigate as new nutritional supplement thioacetamide (TAA)-induced fibrosis. fibrosis model established by intraperitoneal injection TAA (200 mg/kg, every 3 days) for 8 weeks, and oral administration (20 mg/kg 40 daily) after 4 weeks TAA-induced results showed that treatment significantly inhibited activities alanine aminotransferase (ALT) (37.81 ± 7.62 U/L) aspartate (AST) (55.18 10.94 in serum mice, increased levels superoxide dismutase (SOD) glutathione (GSH) while reversed level malondialdehyde (MDA). In addition, histopathological examination illustrated induced inflammatory infiltration, apoptosis fibroatherosclerotic deposition liver, further confirmed western-blot immunofluorescence staining. Moreover, hepatocyte regulating phosphorylation phosphatidylinositol 3-kinase (PI3K), protein kinase-B (AKT) its downstream apoptotic family. Interestingly, staining alpha smooth muscle actin (α-SMA), marker hepatic stellate cell activation, regulated expression transforming growth factor (TGF-β1). Importantly, supplementation with release nuclear kappa-B (NF-κB) signaling pathway pro-inflammatory factors tissue TAA, improved fiber diseases, such tumor necrosis (TNF-α) recombinant rat IL-1β (IL-1β). conclusion, evidence revealed is potential factor, also provides possibility

Language: Английский

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Multifactorial Diseases of the Heart, Kidneys, Lungs, and Liver and Incident Cancer: Epidemiology and Shared Mechanisms

Cancers, Journal Year: 2023, Volume and Issue: 15(3), P. 729 - 729

Published: Jan. 25, 2023

Within the aging population, frequency of cancer is increasing dramatically. In addition, multiple genetic and environmental factors lead to common multifactorial diseases, including cardiovascular disease, chronic kidney obstructive pulmonary metabolic-associated fatty liver disease. recent years, there has been a growing awareness connection between as well how one can affect other, resulting in vicious cycle. Although exact mechanistic explanations behind this remain be fully explored, some progress made uncovering pathologic mechanisms. review, we focus on nature link conditions, specific shared mechanisms, which may represent either preventive or therapeutic targets. Rather than organ-specific interactions, herein mechanisms among explain increased risk. More research subject will highlight significance developing new drugs that target systems rather just

Language: Английский

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Ozone Exposure Controls Oxidative Stress and the Inflammatory Process of Hepatocytes in Murine Models

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 212 - 212

Published: Feb. 8, 2024

(1) Background: Ozone exposure is a promising tool for treating liver damage since it known to control the release of free radicals and increase expression antioxidant enzymes. The objective investigate main intracellular pathways activated after ozone, considering dosage enzymes markers oxidative stress. (2) Methods: This systematic review was performed based on PRISMA guidelines using structured search in MEDLINE (PubMed), Scopus, Web Science. Bias analysis methodological quality assessments were examined SYRCLE Risk tool. (3) Results: Nineteen studies selected. results showed that ozone has protective effect tissue, promoting decrease inflammatory reduction stress tissue. In addition, also promoted an morphological consequences controlling these reducing tissue process areas degeneration necrosis. (4) Conclusions: beneficial models injury through markers. regulates Nrf2/ARE pathway blocks NF-κB pathway.

Language: Английский

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“Effect of Methoxychlor on Liver Function, Lipid Peroxidation, and Antioxidants in Experimental Rats”

Toxicology Reports, Journal Year: 2025, Volume and Issue: 14, P. 101988 - 101988

Published: March 12, 2025

Methoxychlor (MXC), a widely used pesticide, poses significant toxicological risks to various biological systems. It is an environmental contaminant and the only organochlorine pesticide still using instead of DDT. Endocrine disruption MXC also under investigation.This study aimed investigate effects on antioxidant status, lipid peroxidation, liver metabolism in experimental rats. Male Wistar rats were divided into control treatment groups, with latter receiving 150 mg/kg 250 body weight (BW) via oral administration for 30 days. Liver function was assessed by measuring circulating biomarkers, including Alanine Transaminase (ALT), Aspartate (AST), Alkaline Phosphatase (ALP). Oxidative damage evaluated determining Thiobarbituric Acid Reactive Substances (TBARS), hydroperoxide (HYP), other peroxidation markers. Key enzymes involved defense mechanisms analyzed animals. Our results demonstrated increase ALT, AST, ALP levels serum exposed MXC, indicating impaired function. This accompanied elevated further emphasizing oxidative stress. Moreover, activities such as SOD, GPx, CAT markedly reduced MXC-exposed groups compared controls, suggesting compromised system. These findings suggest that methoxychlor exposure disrupts induces stress enhancing thereby depleting natural defenses. highlights potential hepatotoxic underscores role mediating its toxicity.

Language: Английский

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