L-threonine promotes healthspan by expediting ferritin-dependent ferroptosis inhibition in C. elegans

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Nov. 2, 2022

Abstract The pathways that impact longevity in the wake of dietary restriction (DR) remain still ill-defined. Most studies have focused on nutrient limitation and perturbations energy metabolism. We showed L-threonine was elevated Caenorhabditis elegans under DR, supplementation increased its healthspan. Using metabolic transcriptomic profiling worms were fed with RNAi to induce loss key candidate mediators. loss-of-threonine dehydrogenaseincreased healthspan by attenuating ferroptosis a ferritin-dependent manner. Transcriptomic analysis FTN-1 encoding ferritin elevated, implying is an essential mediator promotion. Organismal levels positively correlated chronological aging protected against age-associated through DAF-16 HSF-1 pathways. Our investigation uncovered role distinct universal metabolite, L-threonine, DR-mediated improvement organismal healthspan, suggesting it could be effective intervention for preventing senescence progression age-induced ferroptosis.

Language: Английский

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Protein quality of commercially important edible bivalves

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Feb. 8, 2024

Bivalves are a high-quality source of animal protein for human consumption. In recent years, the demand bivalve proteins has increased dramatically, leading to sharp increase in global production marine bivalves. To date, although amino acid profiles many bivalves have been reported, such information not well organized. Therefore, there is an urgent need comprehensive scientific review quality bivalves, especially commercially important edible this context, study was conducted evaluate general, most rich (> 50% their dry weight) and acids 30 g/100g protein). Although species essential (EAA) (up 50 protein), some very low levels EAA (< 5 Based on AA score, almost all at least two limiting AAs. Most provides delicious flavors with unami, sweetness hint bitterness. The findings only serve as guide selecting appropriate based consumer preferences specific AAs or scores, but also provide potential aquaculture produce higher meet growing high protein.

Language: Английский

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Microbial short-chain fatty acids: a bridge between dietary fibers and poultry gut health — A review

Animal Bioscience, Journal Year: 2022, Volume and Issue: 35(10), P. 1461 - 1478

Published: April 30, 2022

The maintenance of poultry gut health is complex depending on the intricate balance among diet, commensal microbiota, and mucosa, including epithelium superimposing mucus layer. Changes in microflora composition abundance can confer beneficial or detrimental effects fowl. Antibiotics have devastating impacts altering landscape which further leads to antibiotic resistance spread pathogenic populations. By eliciting strategies should be made break down regulatory signals bacteria. optional strategy conferring dietary fibers (DFs) used counterbalance microbiota. DFs are non-starch carbohydrates indigestible by host endogenous enzymes but fermented symbiotic microbiota produce shortchain fatty acids (SCFAs). This one primary modes through interacts communicate with host. majority SCFAs produced large intestine (particularly caecum), where they taken up enterocytes transported portal vein circulation into bloodstream. Recent shreds evidence elucidated that affect modulate tissues organs either activating G-protein-coupled receptors affecting epigenetic modifications genome inducing histone acetylase activities inhibiting deacetylases. Thus, this way, vastly influence promoting energy regulation, mucosal integrity, immune homeostasis, maturation. In review article, we will focus DFs, directly interact microbes lead production SCFAs. Further, discuss current molecular mechanisms how generated, transported, modulated pro-and anti-inflammatory responses against pathogens physiology health.

Language: Английский

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Nutrient sensors and their crosstalk

Experimental & Molecular Medicine, Journal Year: 2023, Volume and Issue: 55(6), P. 1076 - 1089

Published: June 1, 2023

The macronutrients glucose, lipids, and amino acids are the major components that maintain life. ability of cells to sense respond fluctuations in these nutrients is a crucial feature for survival. Nutrient-sensing pathways thus developed govern cellular energy metabolic homeostasis regulate diverse biological processes. Accordingly, perturbations sensing associated with wide variety pathologies, especially diseases. Molecular sensors core within have certain degree specificity affinity intracellular fluctuation each nutrient either by directly binding or indirectly its surrogate molecules. Once changes levels detected, trigger signaling cascades fine-tune processes homeostasis, example, controlling uptake, de novo synthesis catabolism nutrient. In this review, we summarize discoveries on nutrient-sensing explain how those pathway availability mechanisms control Later, further discuss crosstalk between nutrient, which intertwined overall nutrient/metabolic homeostasis.

Language: Английский

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Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

Oil Crop Science, Journal Year: 2023, Volume and Issue: 8(1), P. 16 - 26

Published: March 1, 2023

There is a constant search for biomaterials from natural products like plants food and industrial applications. The work embodied in this report aimed at investigating the effects of microwave-assisted soxhlet extraction (MAE SE) techniques on functional physicochemical quality characteristics Moringa oleifera seed oil proteins extracts. M. ​seeds were ground to fine powders was extracted by using petroleum ether. Quality attributes including yield percent, moisture content, iodine, saponification, specific gravity, viscosity, pH, thiobarbituric acid, acid peroxide values measured. Mineral vitamin contents, chemical/functional groups, fatty (FA) composition, reducing power evaluated. Metabolomics protein defatted analyzed nuclear magnetic resonance (NMR). ​oil MAE SE methods had good (34.25 ​± ​0.0%, 28.75 ​0.0%), low content (0.008 0.011 non-drying unsaturated, moderately saponified, less dense (0.91 ​0.01, 0.92 ​0.02 g mL−1), Newtonian flow, weakly acidic, showed FAs, recorded strong potential long shelf-life, stability against oxidative rancidity enzymatic hydrolysis, very rich deposits micro- macro-nutrients as well water-soluble lipid-soluble vitamins, groups reflective its long- medium-chain triglycerides (LCT MCT). Monounsaturated saturated acids (MUFA SFA) detected has excellent ferric ion power. NMR metabolomic assay revealed presence nine essential amino (EAAs) extract. technique feasible acceptable alternative high throughput ​M. with attributes. study that did not impart any remarkable advantage(s) properties ​seed compared technique.

Language: Английский

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Molecular mechanism and therapeutic significance of essential amino acids in metabolically associated fatty liver disease

The Journal of Nutritional Biochemistry, Journal Year: 2024, Volume and Issue: 126, P. 109581 - 109581

Published: Jan. 13, 2024

Language: Английский

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Impact of Sprouting Process on the Protein Quality of Yellow and Red Quinoa (Chenopodium quinoa)

Molecules, Journal Year: 2024, Volume and Issue: 29(2), P. 404 - 404

Published: Jan. 14, 2024

The demand for plant-based proteins has increased remarkably over the last decade. Expanding availability and variety of protein options shown positive potential. This study aimed to investigate qualitative quantitative changes in amino acids yellow red quinoa seeds (YQ RQ) during a 9-day germination period. results showed that process led an increase total by 7.43% 14.36% YQ RQ, respectively. Both varieties exhibited significant (p < 0.05) increases non-essential essential acids, including lysine, phenylalanine, threonine, tyrosine. content nearly reached standard values found chicken eggs. These were likely attributed impact increasing enzymes activity decreasing anti-nutrient (e.g., saponins). A linear relationship between seeds’ hydration decreased saponins was observed, indicating effect water absorption changing chemical composition plant. sprouts progression; however, sprouted RQ higher rate than (57.67% vs. 43.33%, respectively). Overall, this demonstrates is promising technique enhancing nutritional value seeds, delivering as highly recommended source high-protein grains with notable functional properties.

Language: Английский

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Untargeted metabolomic profiling of serum from client-owned cats with early and late-stage chronic kidney disease

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 27, 2024

Abstract Evaluation of the metabolome could discover novel biomarkers disease. To date, characterization serum client-owned cats with chronic kidney disease (CKD), which shares numerous pathophysiological similarities to human CKD, has not been reported. CKD is a leading cause feline morbidity and mortality, can be lessened early detection appropriate treatment. Consequently, there an urgent need for early-CKD biomarkers. The goal this cross-sectional, prospective study was characterize global, non-targeted versus late-stage compared healthy cats. Analysis revealed distinct separation between cats, early-stage CKD. Differentially abundant lipid amino acid metabolites were primary contributors these differences included central metabolism fatty acids, essential acids uremic toxins. Correlation multiple clinical metadata important monitoring patient treatment (e.g. creatinine, muscle condition score) further illustrates relevance exploring metabolite classes their capacity serve as in both populations.

Language: Английский

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A co-fermentation technology based on small peptide production and protein structure modification increases the edible value of corn gluten meal

LWT, Journal Year: 2024, Volume and Issue: 198, P. 116054 - 116054

Published: April 1, 2024

Although corn gluten meal (CGM) is rich in protein, its application the food industry limited because it contains high concentrations of zein and an unbalanced composition amino acids. In this study, probiotics keratinase were used to conferment a gluten-wheat bran mixture (CGW) with wheat as carbon source. After fermentation under optimal conditions, TA-SCP content CGW was increased from 1.61% 22.67%, small peptide yield reached 52.90%. fermentation, contents essential acids free significantly. The macromolecular proteins effectively degraded, intermolecular force decreased, α-helixs random coils transformed β-sheets β-turns. Animal experiments showed that both fermented (FCGW) wet (WFCGW) products significantly increase crude protein digestibility, trypsin activity acid transporter mRNA expression intestine piglets. addition, changed structure by degrading tightly folded structures into looser structures, leading better digestion absorbtion intestine. Briefly, novel technology effective method for increasing nutritional value CGM ingredient.

Language: Английский

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Widely targeted metabolomic profiling combined with transcriptome analysis provides new insights into amino acid biosynthesis in green and red pepper fruits

Food Research International, Journal Year: 2022, Volume and Issue: 160, P. 111718 - 111718

Published: July 22, 2022

Language: Английский

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