Gut microbiota-derived UDCA enhanced by metformin inhibits FXR to activate autophagy against MCD diet-induced NAFLD in mice

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114471 - 114471

Published: March 22, 2025

Language: Английский

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Effects of Lactobacillus paracei JY062 Postbiotic on Intestinal Barrier, Immunity, and Gut Microbiota

Nutrients, Journal Year: 2025, Volume and Issue: 17(7), P. 1272 - 1272

Published: April 5, 2025

Research on postbiotics derived from probiotic fermented milk bases require further expansion, and the mechanisms through which they exert their effects have yet to be fully elucidated. This study utilized in vitro cell co-culture, digestion, fermentation experiments, combined with targeted T500 technology, elucidate mechanism by postbiotic Pa JY062 safeguards intestinal health. Compared LPS group, boosted phagocytic ability RAW264.7 macrophages, decreased NO levels, alleviated LPS-induced excessive inflammation. suppressed pro-inflammatory cytokines (IL-6, IL-17α, TNF-α) while elevating anti-inflammatory IL-10. It prevented TEER reduction Caco-2 monolayers, FITC-dextran permeability, restored microvilli integrity, upregulated tight junction genes (ZO-1, occludin, claudin-1, E-cadherin). The hydrolysis rate of progressively rose gastrointestinal fluids 0-120 min. At 5 mg/mL, it enriched gut microbiota diversity elevated proportions Limosilactobacillus, Lactobacillus, Pediococcus, Lacticaseibacillus augmenting microbial production acetic acid (120.2 ± 8.08 μg/mL), propionic (9.9 0.35 butyric (10.55 0.13 μg/mL). incorporated αs-casein/β-lactoglobulin hydrolysate (L-glutamic acid, alanine, lysine, tyrosine, phenylalanine, histidine, arginine) mitigate protein allergenic potential harboring bioactive components, including tryptophan metabolites, vitamin B6 (VB6), γ-aminobutyric (GABA). represented a novel demonstrated health-promoting properties. These findings advance current knowledge postbiotic-mediated homeostasis regulation expedite translational development dairy-derived formulations.

Language: Английский

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Beneficial Bacterial Modulation by Gypsum Fibrosum and Terra Flava Usta in Gut Microbiota

Chinese Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Language: Английский

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Exploring the role of gut microbiota in intervertebral disc degeneration: insights from bidirectional Mendelian randomization analysis

European Spine Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Although previous studies have indicated a potential association between the gut microbiota and intervertebral disc degeneration (IVDD), precise nature of this relationship remains unclear. The objective study is to further explore causal IVDD using bidirectional Mendelian randomization approach, with aim identifying microbial characteristics associated IVDD. Using data from genome-wide (GWAS) involving 412 species 227,388 controls 29,508 cases Inverse variance weighted (IVW) was used as primary (MR) analysis, complemented by median, MR-Egger regression, mode simple methods. Extensive sensitivity analyses were performed confirm robustness results assess heterogeneity horizontal pleiotropy. This revealed positive genetic predisposition 6 types through IVW method, indicating that increased levels these may lead higher risk Conversely, found negative effects on IVDD, suggesting protective effect against Reverse MR analysis such possibilities 1 5 relationships microbiota. Cochran's Q test, MR-PRESSO all yielded p-values greater than 0.05, there no significant or pleiotropy in We approach identify various Our findings lay foundation for exploration pathogenesis treatment strategies provide new research biomarkers IVDD-related metabolic

Language: Английский

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Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117198 - 117198

Published: July 26, 2024

The prevalence of obesity is increasingly widespread, resembling a global epidemic. Lifestyle changes, such as consumption high-energy-dense diets and physical inactivity, are major contributors to obesity. Common features this metabolic pathology involve an imbalance in lipid glucose homeostasis including dyslipidemia, insulin resistance adipose tissue dysfunction. Moreover, the importance gut microbiota development susceptibility has recently been highlighted. In recent years, new strategies based on use functional foods, particular bioactive peptides, have proposed counteract outcomes. context, present study examines effects lupin protein hydrolysate (LPH) obesity, dyslipidemia dysbiosis mice fed high-fat diet (HFD). After 12 weeks LPH treatment, gained less weight showed decreased dysfunction compared HFD-fed group. HFD-induced (increased triglycerides, cholesterol LDL concentration) were both counteracted by consumption. Discriminant analysis differentially distributed LPH-treated non-treated mice. HFD reduced ecological parameters, promoted blooming deleterious taxa abundance commensal members. Some these changes corrected Finally, correlation suggested that microbial population could be responsible for improvement conclusion, first show effect improving gain, adiposopathy context diet-induced pointing therapeutic potential peptides diseases.

Language: Английский

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The predictive, preventive, and personalized medicine of depression: gut microbiota and inflammation

The EPMA Journal, Journal Year: 2024, Volume and Issue: 15(4), P. 587 - 598

Published: Sept. 20, 2024

Language: Английский

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Are We Ready to Recommend Capsaicin for Disorders Other Than Neuropathic Pain?

Nutrients, Journal Year: 2023, Volume and Issue: 15(20), P. 4469 - 4469

Published: Oct. 21, 2023

Capsaicin, a lipophilic, volatile compound, is responsible for the pungent properties of chili peppers. In recent years, significant increase in investigations into its has allowed production new formulations and development tools with biotechnological, diagnostic, potential therapeutic applications. Most these studies show beneficial effects, improving antioxidant anti-inflammatory status, inducing thermogenesis, reducing white adipose tissue. Other mechanisms, including food intake intestinal dysbiosis, are also described. this way, possible clinical application such compound expanding every year. This opinion article aims to provide synthesis findings regarding mechanisms by which capsaicin participates control non-communicable diseases as obesity, diabetes, dyslipidemia.

Language: Английский

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Inhibition of MAOB Ameliorated High-Fat-Diet-Induced Atherosclerosis by Inhibiting Endothelial Dysfunction and Modulating Gut Microbiota

Nutrients, Journal Year: 2023, Volume and Issue: 15(11), P. 2542 - 2542

Published: May 30, 2023

In this study, monoamine oxidase B (MAOB) was activated under pathological conditions, and the novel source of cardiovascular reactive oxygen species (ROS). ROS-induced endothelial dysfunction results in sustained chronic vascular inflammation, which is central to atherosclerotic diseases. However, whether MAOB regulates oxidative stress its related mechanism gut microbiota mediates anti-atherosclerosis effect inhibitor remains unclear. our expressions were elevated high-fat diet (HFD) fed mice aortas, but only cells (not smooth muscle cells). small interfering RNA significantly attenuated palmitic-acid (PA)-induced dysfunction. Furthermore, RNA-sequencing data revealed that knockdown decreased levels proinflammatory apoptotic gene induced by PA. Microarray analysis qPCR assay showed miR-3620-5p HFD condition. The dual-luciferase reporter, Western blot confirmed directly regulated binding mRNA 3'UTR. Moreover, inhibition selegiline ameliorated reduced burden HFD-fed ApoE-/- mice. Finally, 16S rRNA sequencing altered community compositional structure microbiota. Specifically, treatment enriched abundance Faecalibaculum Akkermansia, unclassified_f__Lachnospiraceae, Desulfovibrio, Blautia, these genera correlated with serum biochemical indices. Taken together, findings controlled homeostasis, anti-atherosclerotic ameliorating modulating composition function

Language: Английский

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Potential benefits of spicy food consumption on cardiovascular outcomes in patients with diabetes: A cohort study of the China Kadoorie Biobank

Nutrition, Journal Year: 2023, Volume and Issue: 112, P. 112062 - 112062

Published: April 28, 2023

Language: Английский

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Schaftoside from Plumula nelumbinis mitigates “Shanghuo” by inhibiting low‐grade inflammation, diminishing oxidative stress, and enhancing energy metabolism

Food Frontiers, Journal Year: 2024, Volume and Issue: 5(5), P. 2249 - 2261

Published: June 20, 2024

Abstract The study was focused on the therapeutic potential of schaftoside (SS), a key bioactive compound found in Plumula nelumbinis , context “Shanghuo,” an excessive internal heat condition induced by overconsumption capsaicin. “Shanghuo” traditional Chinese medicine is characterized symptoms like skin and mucous membrane redness, swelling, fever, discomfort. It commonly used to describe such as intense thirst, constipation, rapid or surging pulse. In modern medicine, it associated with various factors, including dietary irritants, stress, gut microbiota imbalances, immunological issues. concept Yin–Yang balance pivotal theory medicine. Yin embodies elements stability inhibition, whereas Yang indicative vitality assertiveness. Excessive capsaicin intake can also trigger disrupting body's “Yin Yang.” present study, we isolated SS from P. performed vitro antioxidant assays. exhibited concentration‐dependent increase activity, indicating its combat oxidative stress. animal experiments, rats exposed high dose showed elevated levels inflammatory markers reduced levels. effectively mitigated these restored enzyme inflammation. Furthermore, capsaicin‐treated decreased energy metabolism altered microbiota. treatment enhanced increased abundance intestinal flora, highlighting restore metabolic balance. conclusion, shows promise alleviating intake.

Language: Английский

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