Comparative Study of Prebiotics for Infants Using a Fecal Culture System: Insights into Responders and Non-Responders DOI Open Access

Shijir Mingat,

Tatsuya Ehara,

Hirohiko Nakamura

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(19), P. 3347 - 3347

Published: Oct. 2, 2024

Background: The gut microbiota of breast-fed infants is dominated by infant-type human-residential bifidobacteria (HRB) that contribute to infant health; thus, it crucial develop formulas promote the establishment a enriched with HRB, closely resembling breastfed infants. Methods: We compared various non-digestible prebiotic oligosaccharides and their combinations using fecal culture system explore which candidates could growth all HRB rarely yield non-responders. analysis included lactulose (LAC), raffinose (RAF), galactooligosaccharides (GOS), short- long-chain fructooligosaccharides. Fecal samples were collected from seven aged 1.5–10.2 months cultured each oligosaccharide individually or combinations. Results: No single effectively promoted although GOS other than Bifidobacterium longum subsp. longum. Only LAC/RAF/GOS group evenly HRB. Accordingly, acetate production was higher in cultures supplemented cultures, suggesting superior combination for yields Conclusions: This study can aid developing help align formula-fed

Language: Английский

Unlocking the mysteries of milk oligosaccharides: Structure, metabolism, and function DOI Creative Commons
Qianqian Yao, Yanan Gao, Nan Zheng

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 332, P. 121911 - 121911

Published: Feb. 5, 2024

Milk oligosaccharides (MOs), complex carbohydrates prevalent in human breast milk, play a vital role infant nutrition. Serving as prebiotics, they inhibit pathogen adherence, modulate the immune system, and support newborn brain development. Notably, MOs demonstrate significant variations concentration composition, both across different species within same species. These characteristics of lead to several compelling questions: (i) What distinct beneficial functions do offer how vary along with their structural differences? (ii) In what ways milk differ from those other mammals, factors drive these unique profiles? (iii) are emerging applications MOs, particularly context incorporation into formula? This review delves characteristics, quantification methods, species-specific differences MOs. It highlights critical growth potential applications, providing substantial evidence enhance health

Language: Английский

Citations

14
Interactions of human milk oligosaccharides with the immune system DOI Creative Commons

A. Slater,

Rita M. Hickey, Gavin P. Davey

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 14, 2025

Human milk oligosaccharides (HMOs) are abundant, diverse and complex sugars present in human breast milk. HMOs well-characterized barriers to microbial infection by modulating the microbiome they also thought be nutritionally beneficial infant. The structural variety of over 200 HMOs, including neutral, fucosylated sialylated forms, allows them interact with immune system various ways. Clinically, impact allergic diseases, reducing autoimmune inflammatory responses, offer support preterm infant health. This review examines HMO composition associated immunomodulatory effects, interactions cell receptors gut-associated responses. These properties highlight potential for use early stage development as novel immunotherapeutics. research is rapidly evolving promises innovative treatments immune-related conditions improved health outcomes.

Language: Английский

Citations

1
Advances and challenges for obtaining human milk oligosaccharides: Extraction from natural sources and synthesis by intentional design DOI
Junai Gan, Cuiyan Cao, Bernd Stahl

et al.

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 141, P. 104203 - 104203

Published: Oct. 14, 2023

Language: Английский

Citations

14
Preparatory work for the safety evaluation by EFSA of Human‐identical Milk Oligosaccharides as Novel Foods DOI Open Access

Narges Malih,

Jadwiga Konieczna,

Mar Nafria Fernandez

et al.

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(7)

Published: July 1, 2024

Abstract Background and aims This report describes a scoping literature review as preparatory work for the safety evaluation by EFSA of Human‐identical Milk Oligosaccharides (HiMOs) Novel Foods (NFs). The main objective was to gather information on concentrations Human (HMOs) in human milk, both individual total, combinations/mixtures HiMOs/HMOs commercially available products or those reported literature. Methods A conducted, including search strategy implemented biomedical (PubMed) multidisciplinary (Web Science) databases from 2013 March 2024, supplemented an ad hoc primary studies included two key recent systematic reviews grey searches relevant food business operators' websites. Both secondary were selected determine target HMOs, total concentration HMOs HMO fractions, HiMOs/HMOs. Only with full text published English Spanish eligible, excluding patents, editorials, conference abstracts, books, dissertations. Results structured yielded 2,851 unique references, 218 full‐text 36 additional references reviews. Ultimately, 171 publications review. Fourteen companies related HiMOs identified through In most publications, data multiple groups donors (e.g., based factors like secretor status lactation period) reported. By donors, mean milk (excluding colostrum) 6,740 mg/L, whereas publication 5,341 mg/L. publication, (mg/L, were: LNnT 288, LNT 971, 2'‐FL 1,468, 3‐FL 800, DFL 232, 3'‐SL 212, 6'‐SL 365, LNFP I 738, LNT/LNnT 611. 382, 1064.1, 1685, 907, 303, 225, 435, 773, 882. (mg/L) was: 4,577 neutral; 851 acidic; 4,635 acidic fucosylated; 1,631 α‐1‐3/4‐fucosylated; 3,304 α‐1‐2‐fucosylated; 1,601 non‐fucosylated; 1,505 sialyllated; 2,293 sialyllated. group 3,942 1,032 6,477 1,650 3,748 2,026 1,957 frequent combinations HMOs/HiMOs + (24 products, 32%) (12 16%). Conclusions findings this will support NFs, accordance EC mandate (M‐2023‐00157).

Language: Английский

Citations

5
Human milk oligosaccharides promote intestinal epithelium regeneration independent of the microbiota during necrotizing enterocolitis DOI

Andrea Zito,

Richard Wu, Bo Li

et al.

Pediatric Surgery International, Journal Year: 2024, Volume and Issue: 40(1)

Published: Jan. 12, 2024

Language: Английский

Citations

4
Regioselective Enzymatic Galactosylation Enabled Divergent Synthesis of Asymmetrical Biantennary Human Milk Oligosaccharides DOI
Hui Xia,

Kan Zhong,

Yun Li

et al.

ACS Catalysis, Journal Year: 2024, Volume and Issue: 14(17), P. 13390 - 13399

Published: Aug. 23, 2024

Human milk oligosaccharides (HMOs) are essential nutritional and bioactive components of human breast milk, conferring numerous beneficial effects on neonatal health. The HMO repertoire comprises over 200 unique glycan structures, more than half them branched oligosaccharides. Owing to the structural diversity complexity, synthesis HMOs remains challenging, which significantly hinders their functional study. We herein present a highly efficient biomimetic approach for rapid scaled-up chemoenzymatic asymmetrical biantennary HMOs. Taking advantage inherent regioselectivity panel bacterial galactosyltransferases, two pentasaccharide intermediates were obtained through regioselective enzymatic β1,3- or β1,4-galactosylation readily available chemically synthesized symmetrical tetrasaccharide precursor. further diversified by sequential multienzyme cascade reactions afford library 20 structurally well-defined lacto-N-hexaose (LNH) lacto-N-neo-hexaose (LNnH) series in less 5 linear steps.

Language: Английский

Citations

4
Improving fat globule structure to narrow metabolite gap between human milk and infant formulae DOI
Yue Sun, Xueying Zhang, Jiayu Li

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 471, P. 142797 - 142797

Published: Jan. 6, 2025

Language: Английский

Citations

0
Recent advances in the science of human milk oligosaccharides DOI Creative Commons
Tadasu Urashima, Katsumi Ajisaka, Tetsuro Ujihara

et al.

BBA Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100136 - 100136

Published: Jan. 1, 2025

Language: Английский

Citations

0
Technical review by the ESPGHAN special interest group on gut microbiota and modifications on the health outcomes of infant formula supplemented with manufactured human milk oligosaccharides DOI Open Access
Iva Hojsak, Ener Çağrı Dinleyici, Chris H.P. van den Akker

et al.

Journal of Pediatric Gastroenterology and Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract This technical review, one of five developed by the European Society for Pediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) special interest group (SIG) on gut microbiota modifications (GMM), supports creation a position paper use biotic‐supplemented formulas, including those containing human milk oligosaccharides (HMOs) produced through chemical synthesis or microbial biotechnology. Though these are identical to HMOs found in milk, they do not originate from it. Therefore, we used human‐identical (HiMOs). review focuses clinical outcomes related supplementation infant formulas with HiMOs. The ESPGHAN SIG‐GMM conducted systematic evaluate HiMO‐supplemented healthy infants (0–12 months) published before 2024. Six RCTs two mechanistic substudies met inclusion criteria investigated different combinations HiMOs added formula. studied so far show no difference compared control formula such as: anthropometric data, regurgitation‐related symptoms, crying, fussiness, colic. A specific combination HMO‐analogues (2′fucosyllactose [FL], 3‐FL, lacto‐N‐tetraose [LNT], 3′‐sialyllactose [SL], 6′‐SL) suggest softer stool consistency more frequent defecation presumable infants, but studies also highest amount HiMOs; however, relevance this finding remains uncertain. Regarding infection prevention, clear conclusion can be drawn. There was tolerability safety concerns were raised HiMO far. report serves as background formulating recommendations HiMOs‐supplemented

Language: Английский

Citations

0
Human milk oligosaccharides modulating inflammation in infants, adults and older individuals - from concepts to applications DOI Creative Commons
Robert Häsler, Marta Mikš‐Krajnik, Danica Bajic

et al.

Advances in Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 100433 - 100433

Published: April 1, 2025

Language: Английский

Citations

0