Cardiac diastolic dysfunction by cigarette smoking is associated with mitochondrial integrity in the heart DOI Open Access
Lily Slotabec, Hao Wang, Blaise Seale

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(14)

Published: July 24, 2024

Abstract Cigarette smoking behaviors are harmful and cause one out of ten deaths due to cardiovascular disease. As population sizes grow number cigarette smokers increases, it is vital that we understand the mechanisms leading heart failure in smokers. We have reported metabolic regulation a histone deacetylase, SIRT1, modulates mitochondrial function under stress. Given this conclusion, hypothesized chronic led dysfunction via reduction SIRT1. Mice were randomly organized into or nonsmoking groups, group received smoke exposure for 16 weeks. Following 16‐week exposure, diastolic was impaired as compared sham, indicated by significant increase E/e’. The electrical also sham group, increased PR interval decreased QTc interval. This not accompanied fibrosis mouse hearts, although samples from human their nonsmoker counterparts. well dysfunction, mitochondria showed significantly function, evidenced decreases all parameters measured stress test. further found biochemical evidence level SIRT1 left ventricles both groups Data study indicate levels associated with caused compromised integrity.

Language: Английский

Fucoidan Oligosaccharide Supplementation Relieved Kidney Injury and Modulated Intestinal Homeostasis in D-Galactose-Exposed Rats DOI Open Access
Jing Shi, Yan Xu, Kening Zhang

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(2), P. 325 - 325

Published: Jan. 17, 2025

Background/Objectives: A fucoidan oligosaccharide (FOS), a potent compound derived from algae, is known for its diverse biological activities, including prebiotic activity, anticancer and antioxidative properties, has demonstrated supportive therapeutic effects in treating kidney ailments. This study was conducted to explore the protective influence of FOS on damage due aging induced by D-galactose Sprague Dawley (SD) rats. Methods: The low-dose group administered (100 mg/kg) gavage, high-FOS received (200 gavage. Results: findings showed that could effectively mitigate improve pathological condition tissues caused D-gal enhance function. Intervention with significantly reduced serum creatinine, uric acid, urea nitrogen levels, compared model group. mechanism D-gal-induced injury may be inhibit oxidative stress impaired mitochondrial function downregulating AMPK/ULK1 signaling pathway. also modulate expression autophagy-related proteins (Beclin-1, P62, LC3II/LC3I), thereby excessive mitophagy kidney. Furthermore, protect against preserving intestinal homeostasis. decreased lipopolysaccharide levels enhanced mucosal barrier upregulated abundances Bacteroidota, Muribaculaceae, Lactobacillus, while it Firmicutes, NK4A136_group, Lachnospiraceae_NK4A136_group. supplementation modulated gut microbiota composition, increasing beneficial bacteria reducing detrimental ones, potentially contributing improved Conclusions: safeguard renal D-gal-exposed rats inhibiting mitophagy, function, regulating

Language: Английский

Citations

1
Mitochondrial biogenesis microcarriers break membrane barriers for endogenous nucleus pulposus repair DOI
Xiaohu Li, Hongze Chang, Libin Pang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160668 - 160668

Published: Feb. 1, 2025

Citations

0
Astragaloside IV alleviated bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and fecal metabolism DOI Creative Commons
Huichao Wang, Zhongyue Huang, Guangnan Chen

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

Astragaloside IV (AS-IV) is one of the most potent components Astragalus. It has been reported to promote bone formation and inhibit osteoclastogenesis, suggesting its potential as a candidate for prevention treatment postmenopausal osteoporosis (PMOP). The gut microbiota may play crucial role in mediating effects AS-IV. To investigate impact on efficacy AS-IV treating PMOP. Mice were randomly divided into three groups: Sham, ovariectomy (OVX), AS-IV-treated OVX group (80 mg/kg). Bone loss was evaluated using Micro-CT histopathology. Immunohistochemistry assessed specific markers. Inflammatory levels measured by enzyme-linked immunosorbent assay (ELISA). Intestinal barrier function examined via colonic histopathology immunohistochemistry. Gut composition analyzed 16S rDNA sequencing, while metabolomic profiling identified key metabolites. Correlation analysis performed explore relationships between differential bacteria, metabolites, loss. improved femur microarchitecture modulated turnover mice. strengthened intestinal decreased permeability. This compound reduced oxidative stress serum marrow inflammatory cytokine production. sequencing revealed that composition, showed pathways related hormone biosynthesis, D-amino acid metabolism, galactose metabolism. study provides new insights use PMOP, highlighting metabolites regulatory factors AS-IV's therapeutic effects.

Language: Английский

Citations

0
Absorption Patterns of Fucoidan Oligosaccharides from Kjellmaniella crassifolia in the Caco-2 Monolayer Cell Model and Their Pharmacokinetics in Mice DOI Creative Commons

Zhiying Xu,

Qing Xia, Liu Li

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1486 - 1486

Published: April 24, 2025

Oligosaccharides possess characteristics such as low molecular weight, good solubility, and high bioavailability, which make them better absorbed than fucoidan. This study hypothesizes that fucoidan oligosaccharides can be by intestinal epithelial cells quickly enter the bloodstream, with a rapid absorption rate. In this study, were obtained through acid degradation Bio Gel column separation. By analyzing chemical composition smaller weights simpler monosaccharide compositions selected for further research. A cell model pharmacokinetic studies in mice established to analyze patterns of oligosaccharides. The results showed after separation, high-molecular-weight SPF1 weight range 1.63 × 104 2.14 105 Da low-molecular-weight SPF2 244.22 1545.36 obtained. transport uptake experiments, was positively correlated time negatively concentration. rates ranged from 20% 70%, Papp values greater 1 10−5 cm/s. pharmacokinetics blood concentration simulated analyzed using DAS 2.0, indicated exhibited vivo vitro. Therefore, are more easily absorbed, provides theoretical basis application

Language: Английский

Citations

0
Cardiac diastolic dysfunction by cigarette smoking is associated with mitochondrial integrity in the heart DOI Open Access
Lily Slotabec, Hao Wang, Blaise Seale

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(14)

Published: July 24, 2024

Abstract Cigarette smoking behaviors are harmful and cause one out of ten deaths due to cardiovascular disease. As population sizes grow number cigarette smokers increases, it is vital that we understand the mechanisms leading heart failure in smokers. We have reported metabolic regulation a histone deacetylase, SIRT1, modulates mitochondrial function under stress. Given this conclusion, hypothesized chronic led dysfunction via reduction SIRT1. Mice were randomly organized into or nonsmoking groups, group received smoke exposure for 16 weeks. Following 16‐week exposure, diastolic was impaired as compared sham, indicated by significant increase E/e’. The electrical also sham group, increased PR interval decreased QTc interval. This not accompanied fibrosis mouse hearts, although samples from human their nonsmoker counterparts. well dysfunction, mitochondria showed significantly function, evidenced decreases all parameters measured stress test. further found biochemical evidence level SIRT1 left ventricles both groups Data study indicate levels associated with caused compromised integrity.

Language: Английский

Citations

0