Hawthorn leaf and its extract alleviate high-fat diet-induced obesity and modulate gut microbiome in mice

Current Research in Food Science, Journal Year: 2025, Volume and Issue: 10, P. 101025 - 101025

Published: Jan. 1, 2025

Obesity has emerged as a global health issue with its prevalence continuously increasing and being associated multiple comorbidities. Although existing medications are effective, they often come significant side effects, making dietary therapy an advantageous alternative. Hawthorn leaves their active component, vitexin, have shown potential in regulating lipid metabolism improving gut microbiota imbalance. This study utilized high-fat diet-induced obese mouse model, administering different doses of hawthorn vitexin for 13 weeks, employed 16S rRNA sequencing metabolomics to analyze the composition metabolites. The results demonstrated that significantly slowed body weight gain, improved glucose tolerance, regulated blood levels, downregulated expression obesity-related gene mice (ppar-α, ppar-γ, fas). Additionally, treatment groups showed improvement diversity. Both increased abundance Kineothrix, Paramuribaculum, Lawsonibacter (which belong Bacillota phylum) Olsenella (Actinobacteria phylum), while reducing Anaerotignum (Bacillota phylum). Moreover, treatments may alleviate symptoms by fecal content testosterone propionate, formoterol, isoleucyl-prolyl-proline, decreasing Trolox. These findings highlight functional foods obesity management modulating pathways, offering promising approach.

Language: Английский

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Adipocytokine Protein Expression from Visceral Fat Differs Significantly Based on Diet, Sex, and Age in C3H/HeJ Mice Fed Long-Term, High-Fat Diets, ± Ammonium-Hydroxide-Supplemented Dietary Protein

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 218 - 218

Published: March 23, 2025

(1) Background: Overconsumption of processed meats, fats, and carbohydrates drives the obesity epidemic in USA. Associated with this are increases metabolic diseases, such as type 2 diabetes, cardiovascular disease, cancer. In study, protein levels adipocytokines isolated from visceral fat mice fed high-fat diets proteins modified through ammonium supplementation were analyzed to determine changes that occur a result dietary source its modification based on age or sex. (2) Methods: Male female C3H/HeJ randomized into six customized diets—Group 1: CCN = Control Chow (CC) + Ammonium Hydroxide Enhancement (AHE); Group 2: CC Chow; 3: HFBN High Fat (HF) AHE Dietary Beef; 4: HFB HF 5: HFCN Casein; 6: HFC Casein. Mice censored at six-month intervals, was collected for analysis. This study highlights sex- age-related cellular adipocytokine expression 12 18 months. (3) Results: When compared casein, dietary-beef-fed showed increased adiponectin, leptin, MCP-1. casein diets, high content correlated pro-inflammatory MCP-1, resistin, VEGF-A, TIMP-1. Sex-related differences observed MCP-1 levels. decreased Age-related VEGF-A. (4) Conclusions: Our results indicate plays critical role determining WAT. Furthermore, shows addition (beef casein), also impact relative both anti-inflammatory sex over time, leptin identified most frequently affected.

Language: Английский

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The Modification of Dietary Protein with Ammonium Hydroxide Enhancement Improves Longevity and Metabolic Outcomes in a Sex-Dependent Manner

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2787 - 2787

Published: Aug. 21, 2024

(1) Background: Dietary protein is a key component of all dietary patterns. It has been demonstrated that there are subtle differences in health implications associated with the source consumed. This study examined sources (DPSs) long-term diet-induced obesity ± ammonium hydroxide enhancement (AHE) and its role improving outcomes. (2) Methods: Over 18 months, 272 C3H/HeJ mice (136 male 136 female) were monitored on high-fat diets varying DPSs AHE. Mice for weekly change total mass, as well 6-month assessments lean fat mass. At each assessment, cohort (~8 per diet sex) was censored cross-sectional examination organ function. (3) Results: Longevity improved females fed AHE diets, regardless DPSs. Females' measures mass markedly elevated casein compared to beef Females + reduced increased aging. In males, diet-fed showed marked improvement longevity at 6 months. (4) Conclusions: demonstrates modification by attenuates negative impacts HF both males sex-dependent manner. Furthermore, results from this emphasize importance identifying utilization proteins sex- age-related manner demonstrate potential DPS intervention.

Language: Английский

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