Comparison of Glucose Metabolizing Properties of Enterobacterial Probiotic Strains In Vitro DOI Open Access

Jules Balanche,

Emilie Lahaye, Lisa Bremard

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2677 - 2677

Published: Aug. 13, 2024

Before the absorption in intestine, glucose encounters gut bacteria, which may serve as a barrier against hyperglycemia by metabolizing glucose. In present study, we compared capacity of enterobacterial strains to lower levels an vitro model nutrient-induced bacterial growth. Two probiotic strains, Hafnia alvei HA4597 (H. alvei) and Escherichia coli (E. coli) Nissle 1917, well E. K12, were studied. To mimic growth gut, planktonic culture was supplemented twice daily Luria Bertani milieu with or without 0.5% Repeated nutrient provision resulted incremental bacteria. However, presence glucose, maximal both but not H. inhibited. When added medium, continuous decrease its concentration observed during each feeding phase. At highest density, displayed more efficient consumption accompanied pronounced downregulation transporters’ expression than K12. Thus, study reveals that strain is resilient maintain consumption. This experimental approach offers new strategy for identification probiotics increased capacities potentially useful prevention co-treatment type 2 diabetes.

Language: Английский

Gut microbiota in health and disease: advances and future prospects DOI Creative Commons
Y J Zhang, Hong Wang, Yingpeng Sang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Nov. 20, 2024

Abstract The gut microbiota plays a critical role in maintaining human health, influencing wide range of physiological processes, including immune regulation, metabolism, and neurological function. Recent studies have shown that imbalances composition can contribute to the onset progression various diseases, such as metabolic disorders (e.g., obesity diabetes) neurodegenerative conditions Alzheimer's Parkinson's). These are often accompanied by chronic inflammation dysregulated responses, which closely linked specific forms cell death, pyroptosis ferroptosis. Pathogenic bacteria trigger these death pathways through toxin release, while probiotics been found mitigate effects modulating responses. Despite insights, precise mechanisms influences diseases remain insufficiently understood. This review consolidates recent findings on impact immune‐mediated inflammation‐associated conditions. It also identifies gaps current research explores potential advanced technologies, organ‐on‐chip models microbiome–gut–organ axis, for deepening our understanding. Emerging tools, single‐bacterium omics spatial metabolomics, discussed their promise elucidating microbiota's disease development.

Language: Английский

Citations

8
Associations of Microbiota and Nutrition with Cognitive Impairment in Diseases DOI Open Access
Ewelina Młynarska, Paulina Jakubowska, Weronika Frąk

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3570 - 3570

Published: Oct. 21, 2024

Recent research highlights the growing interest in impact of nutrition on cognitive health and function disease, as dietary habits are increasingly recognized crucial factors relation to brain function. This focus is especially important given rising prevalence neurodegenerative diseases decline associated with poor choices. Links now being sought between microbiota gut-brain axis. Mechanisms proposed that include low-grade chronic neuroinflammation, influence short-chain fatty acids, or disruption glial cells transmitters brain.

Language: Английский

Citations

4
A randomized multi-arm open labelled comparative clinical trial report of Pankajakasthuri DiabetEaze powder, a novel polyherbal formulation on the nutritional management and glycemic control in type 2 diabetic and prediabetic patients DOI Creative Commons
Shan Sasidharan,

Kasthuri Nair A,

R. Lekshmi

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42631 - e42631

Published: Feb. 1, 2025

Language: Английский

Citations

0
Protective Effects of Polygonatum sibiricum Polysaccharides Against Type 2 Diabetic Mice Induced by High-Fat Diet and Low-Dose Streptozotocin DOI Creative Commons

Q Li,

Jianbo Cheng, Yangyang Sun

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(4), P. 255 - 255

Published: March 28, 2025

Polysaccharides possessing hypoglycemic effects have shown promising results in treating diabetes. Polygonatum sibiricum polysaccharide (PSP) is one of the most active ingredients Chinese medicine P. Redoute with many biological activities. However, its efficacy alleviating type 2 diabetes mellitus (T2DM) remains unexplored. Our aim to evaluate protective effect PSP against T2DM by measuring body weight and serum biochemical indicators, examining histopathological images pancreatic liver tissues, detecting fecal short-chain fatty acid (SCFA) content, analyzing intestinal flora diversity microbiota structure mice. The findings indicated that treatment mice could obviously decrease fasting blood glucose insulin levels, ameliorate tolerance, resistance, lipid, inflammatory factor attenuate damage, increase SCFA content. In addition, modulate composition gut mice, resulting relative abundance Firmicutes decreasing Bacteroidetes increasing, along beneficial significantly especially SCFA-producing bacteria. indicate administration protected controlling disordered glucolipid metabolism modulating microbiota, which provides a valuable strategy for utilization treat T2DM.

Language: Английский

Citations

0
Diabetic neuropathy: cutting-edge research and future directions DOI Creative Commons
Yang Yang, Bing Zhao,

Yuanzhe Wang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: April 24, 2025

Abstract Diabetic neuropathy (DN) is a prevalent and debilitating complication of diabetes mellitus, significantly impacting patient quality life contributing to morbidity mortality. Affecting approximately 50% patients with diabetes, DN predominantly characterized by distal symmetric polyneuropathy, leading sensory loss, pain, motor dysfunction, often resulting in diabetic foot ulcers lower-limb amputations. The pathogenesis multifaceted, involving hyperglycemia, dyslipidemia, oxidative stress, mitochondrial inflammation, which collectively damage peripheral nerves. Despite extensive research, disease-modifying treatments remain elusive, current management primarily focusing on symptom control. This review explores the complex mechanisms underlying highlights recent advances diagnostic therapeutic strategies. Emerging insights into molecular cellular pathways have unveiled potential targets for intervention, including neuroprotective agents, gene stem cell therapies, innovative pharmacological approaches. Additionally, novel tools, such as corneal confocal microscopy biomarker-based tests, improved early detection intervention. Lifestyle modifications multidisciplinary care strategies can enhance outcomes. While significant progress has been made, further research required develop therapies that effectively halt or reverse disease progression, ultimately improving lives individuals DN. provides comprehensive overview understanding future directions management.

Language: Английский

Citations

0
Gut Microbiota and Metabolic Dysregulation in Elderly Diabetic Patients: Is There a Gender-Specific Effect DOI Open Access
Magdalena Piłot, Sylwia Dzięgielewska-Gęsiak, Katarzyna Walkiewicz

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(9), P. 3103 - 3103

Published: April 30, 2025

Background/Objectives: The aim of this study was to qualitatively and quantitatively assess the bacterial domain gut microbiome in elderly patients with type 2 diabetes (T2D), a focus on sex differences, glycemic control, lipid disorders. Methods: This included 60 older adults T2D (38 women 22 men) treated metformin or combination insulin. microbiota profiled using 16S rRNA gene sequencing. Statistical analyses, including correlation analysis multiple regression, were performed identify associations between microbial taxa, sex, metabolic parameters. Results: No statistically significant differences alpha beta diversity observed sexes. Multiple regression indicated positive relationship Tenericutes HbA1c male participants (β = 2.22931, CI [0.75, 3.70], R 0.67; R2 0.36; unadjusted p 0.0052; adjusted 0.0496). In female participants, G0′ −2.24107, [−3.19, −1.30], 0.78; 0.58; 0.00003; 0.0005) −1.86670, [−2.61, −1.12], 0.00001; 0.0003) correlated negatively Verrucomicrobia as well −1.90427, [−2.95, −0.85], 0.46; 0.17; 0.0008; 0.007) −1.69561, [−2.52, −0.87], 0.0002; 0.002) OD1 bacteria, known Parcubacteria. Conclusions: population diabetes, biological did not significantly affect diversity. However, several exploratory taxa parameters differed men women, suggesting that may influence specific aspects microbiota—metabolism interactions. These preliminary findings underscore importance considering both age- sex-related factors when investigating context diabetes.

Language: Английский

Citations

0
Gut Microbiota and Gut–Brain Axis in Hypertension: Implications for Kidney and Cardiovascular Health—A Narrative Review DOI Open Access
Ewelina Młynarska, Jakub Wasiak, Agata Gajewska

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(23), P. 4079 - 4079

Published: Nov. 27, 2024

Introduction: Arterial hypertension is a major contributor to wide range of health complications, with cardiac hypertrophy and chronic kidney disease being among the most prevalent. Consequently, novel strategies for treatment prevention are actively explored. Recent research has highlighted potential link between gut–brain axis. A bidirectional communication microbiota brain via vagus nerve, enteric nervous system, hypothalamus–pituitary–adrenal axis, secreted short-chain fatty acids, neurotransmitter metabolism. Materials methods: comprehensive literature search was conducted using databases such as PubMed identify studies exploring relationship gut hypertension, along effects dietary interventions probiotics on blood pressure regulation. Discussion: Studies in both animal models human subjects have demonstrated strong correlation alterations composition development hypertension. By influencing pressure, can potentially affect progression cardiovascular disorders. Modulating through shown promise regulating reducing systemic inflammation, offering approach managing Diets Mediterranean diet, which rich polyphenols omega-3 acids low sodium, promote growth beneficial bacteria that support health. Additionally, been found enhance barrier function, reduce modulate Renin–Angiotensin System, all contribute lowering pressure. Conclusions: Further needed determine mechanisms action The aim this study evaluate influence regulation hypertension-related

Language: Английский

Citations

2
Comparison of Glucose Metabolizing Properties of Enterobacterial Probiotic Strains In Vitro DOI Open Access

Jules Balanche,

Emilie Lahaye, Lisa Bremard

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2677 - 2677

Published: Aug. 13, 2024

Before the absorption in intestine, glucose encounters gut bacteria, which may serve as a barrier against hyperglycemia by metabolizing glucose. In present study, we compared capacity of enterobacterial strains to lower levels an vitro model nutrient-induced bacterial growth. Two probiotic strains, Hafnia alvei HA4597 (H. alvei) and Escherichia coli (E. coli) Nissle 1917, well E. K12, were studied. To mimic growth gut, planktonic culture was supplemented twice daily Luria Bertani milieu with or without 0.5% Repeated nutrient provision resulted incremental bacteria. However, presence glucose, maximal both but not H. inhibited. When added medium, continuous decrease its concentration observed during each feeding phase. At highest density, displayed more efficient consumption accompanied pronounced downregulation transporters’ expression than K12. Thus, study reveals that strain is resilient maintain consumption. This experimental approach offers new strategy for identification probiotics increased capacities potentially useful prevention co-treatment type 2 diabetes.

Language: Английский

Citations

0