Initial Glutathione Depletion During Short-Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the γ-Glutamyl Cycle DOI Creative Commons
Filippo Giorgio Di Girolamo, Filippo Mearelli,

Mariella Sturma

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1430 - 1430

Published: Nov. 21, 2024

Hypokinesia triggers oxidative stress and accelerates the turnover of glutathione system via γ-glutamyl cycle. Our study aimed to identify regulatory checkpoints controlling intracellular levels. We measured intermediate substrates cycle in erythrocytes from 19 healthy young male volunteers before during a 10-day experimental bed rest. Additionally, we tracked changes levels specific metabolite ratios up 21 days Using gas chromatography-mass spectrometry internal standard technique, observed 9 ± 9% decrease first 5 rest, followed by an 11 increase 5th 10th day, nearly returning baseline ambulatory The cysteinyl-glycine-to-glutathione ratio, reflecting cyclotransferase activity (a key enzyme breakdown), rose 14 22% then fell 10 14% over subsequent days, again approaching cysteine-to-cysteine indicative cysteine synthetase (crucial for synthesis), increased 12 30% on day 29 41% results rest confirm those seen 10. By calculating ratio product concentration precursor concentration, assessed efficiency these enzymes turnover. These were corroborated directly measuring synthesis degradation rates vivo using stable isotope techniques. findings reveal significant kinetics initial potential therapeutic targets maintaining

Language: Английский

Pharmacological approaches to enhance mitochondrial biogenesis: focus on PGC-1Α, AMPK, and SIRT1 in cellular health DOI

Ahmet Alperen Palabiyik,

Esra Palabıyık

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Feb. 28, 2025

Language: Английский

Citations

3
Aqueous extract of Atractylodes macrocephala Koidz. improves dexamethasone-induced skeletal muscle atrophy in mice by enhancing mitochondrial biological function DOI Creative Commons
Mingzhu Ye, Lai Peng, Yajing Fang

et al.

Experimental Gerontology, Journal Year: 2025, Volume and Issue: 201, P. 112693 - 112693

Published: Jan. 29, 2025

The study aims to investigate the therapeutic effects of aqueous extract Atractylodes macrocephala Koidz. (AEA) on dexamethasone (Dex) -induced sarcopenia in mice and explore its possible mechanisms action. This utilized bioinformatics analysis primary pathogenic age-related Dex-induced muscle atrophy. In animal experiments, a mouse model atrophy was established using Dex, different doses AEA were administered for treatment. evaluated through tests motor ability histological analysis, molecular predicted by verified measuring expression levels related genes. Bioinformatics suggests that there may be shared mitochondrial function structure between Dex significantly reduced mass, function, cross-sectional area fibers mice, also induced changes fiber types. contrast, ameliorated aforementioned atrophic caused Dex. modulation biogenesis dynamics crucial mechanism which exerts anti-sarcopenia effects. can alleviate symptoms skeletal improving indicating potential clinical application prevention treatment sarcopenia.

Language: Английский

Citations

0
Diabetes and Sarcopenia: Intersection of Co Morbid Conditions DOI Open Access
Byron J. Hoogwerf

OBM Geriatrics, Journal Year: 2025, Volume and Issue: 09(01), P. 1 - 23

Published: Feb. 20, 2025

Sarcopenia is more common in persons with type 2 diabetes mellitus (T2DM) than without diabetes. There overlap of some pathophysiologic features sarcopenia and T2DM. In addition, the risk for associated complications T2DM including renal disease, cardiovascular disease neuropathy. Glycemic control may have benefits on sarcopenia. The specific effects glucose lowering agents suggest that metformin, insulin, thiazolidinediones GLP1 receptor agonists favorable while SGLT2 inhibitors an adverse effect. However, RA’s been outcomes increased heart failure. Thus risk/benefit ratio overall key to selection agents. Nutrition guidelines generally align recommendations Data aerobic resistance exercise patients very limited, but data a benefit exercises.

Language: Английский

Citations

0
Impact of Paraspinal Sarcopenia on Clinical Outcomes in Intervertebral Disc Degeneration Patients Following Percutaneous Transforaminal Endoscopic Lumbar Discectomy DOI Creative Commons

Tianci Fang,

Zhancheng Xue, Quan Zhou

et al.

Orthopaedic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

ABSTRACT Objective The paraspinal muscles are a crucial component of the spine's extrinsic stabilization system. While impact muscle sarcopenia on patient‐reported outcome measures (PROMs) after lumbar surgery is well known, its effects following percutaneous transforaminal endoscopic discectomy (PTED) have not been investigated. To investigate prognostic value preoperative long‐term PROMs PTED, and to identify independent predictors chronic postoperative low back pain. Methods In this retrospective cohort study, 145 patients who underwent PTED for disc herniation (2017–2022) were stratified into ( n = 52) non‐sarcopenia 93) groups using sex‐specific psoas index (PMI) thresholds (male: < 6.36 cm 2 /m ; female: 3.92 ). Preoperative MRI/CT was used quantify parameters, including PMI, multifidus (MMI), erector spinae (EMI), Goutallier‐classified fat infiltration (FI) severity (Grades 0–4), density (MMD). Primary outcomes assessed via visual analog scale (VAS; 0–10) Oswestry disability (ODI; 0%–100%) at preoperative, 1‐month, 6‐month, final follow‐up (mean 65.6 weeks). Multivariate logistic regression performed pain (defined as VAS ≥ 4 follow‐up). Results study comprised (69 female, 76 male; mean age: 50.1 ± 7.6 years). group exhibited significantly lower indices (PMI: 4.55 vs. 7.48 , p 0.001, MMI: 2.61 0.80 3.66 0.94 EMI: 9.72 2.46 12.54 2.27 0.001) higher FI 0.05). At follow‐up, reported worse (VAS: 3.04 1.25 2.31 1.50, 0.004) (ODI: 28.33 6.61 21.57 7.28, 0.001). analysis identified BMI (OR 1.319), PMI 0.745), MMI 0.454), moderate/severe 7.036) (all Conclusion Paraspinal sarcopenia, particularly degeneration, modifiable determinant PTED. quality assessment combined with targeted rehabilitation may optimize outcomes.

Language: Английский

Citations

0
Easy-to-Use Guidelines on Protein Intake and Physical Activity Recommendations Derived from the COGFRAIL Study and the Toulouse Frailty Clinic DOI Open Access

Éva Peyrusqué,

Gabor Abellán van Kan, Patricia Álvarez-Rodríguez

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(8), P. 1294 - 1294

Published: April 8, 2025

Background/Objectives: In primary care, tailored physical activity and nutritional counselling are scarce for older adults. Several challenges contribute to this issue, the obstacle being limited access expert healthcare providers. The purpose of study was propose a quick, easy-to-implement case-finding tool offering straightforward overcome these barriers. Methods: Cross-sectional, baseline analysis performed on 277 participants Cognitive Function Amyloid Marker in Frail Older Adults (COGFRAIL) study, aged 70 years with mild cognitive impairment (mini-mental state examination score ≥ 20) autonomy daily living activities (ADL 4). Body composition assessed using dual-energy X-ray absorptiometry, function short performance battery (SPPB), nutrition mini assessment (MNA). A structured dietary interview conducted collect data typical intake pattern. second database 725 autonomous frail adults from Frailty clinic used test robustness findings. Results: Participants MNA scores < 24/30 SPPB 6/12 presented high percentage protein (74.1%) caloric (66.7%) deficiency compared other categories. Based standard recommendations, age, weight, category had protein-caloric deficit −19.4 ± 22.7 g −225.5 430.1 Kcal. Conclusions: data, an easy-to-use algorithm is suggested. This could serve as effective guiding community-dwelling

Language: Английский

Citations

0
Glutamate and Its Derivatives in Regulation of Peripheral Cholinergic Neurotransmission DOI

N. S. Fedorov,

Egor Nevsky,

A. R. Tokmakova

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 61(2), P. 369 - 386

Published: March 1, 2025

Language: Английский

Citations

0
Research Progress of Sarcopenia in the Elderly DOI

珠 朱

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(05), P. 139 - 146

Published: Jan. 1, 2025

Language: Английский

Citations

0
Initial Glutathione Depletion During Short-Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the γ-Glutamyl Cycle DOI Creative Commons
Filippo Giorgio Di Girolamo, Filippo Mearelli,

Mariella Sturma

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1430 - 1430

Published: Nov. 21, 2024

Hypokinesia triggers oxidative stress and accelerates the turnover of glutathione system via γ-glutamyl cycle. Our study aimed to identify regulatory checkpoints controlling intracellular levels. We measured intermediate substrates cycle in erythrocytes from 19 healthy young male volunteers before during a 10-day experimental bed rest. Additionally, we tracked changes levels specific metabolite ratios up 21 days Using gas chromatography-mass spectrometry internal standard technique, observed 9 ± 9% decrease first 5 rest, followed by an 11 increase 5th 10th day, nearly returning baseline ambulatory The cysteinyl-glycine-to-glutathione ratio, reflecting cyclotransferase activity (a key enzyme breakdown), rose 14 22% then fell 10 14% over subsequent days, again approaching cysteine-to-cysteine indicative cysteine synthetase (crucial for synthesis), increased 12 30% on day 29 41% results rest confirm those seen 10. By calculating ratio product concentration precursor concentration, assessed efficiency these enzymes turnover. These were corroborated directly measuring synthesis degradation rates vivo using stable isotope techniques. findings reveal significant kinetics initial potential therapeutic targets maintaining

Language: Английский

Citations

1