Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius DOI Creative Commons
Roberta Perego, Gabriele Meroni, Piera Anna Martino

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 670 - 670

Published: Dec. 20, 2024

Staphylococcus pseudintermedius (SP) is a commensal and opportunistic pathogen of skin mucosal surfaces, isolated from healthy dogs canine pyoderma cases. It has recently gained attention due to its increasing antibiotic resistance. Platelet-rich plasma (PRP) biological product, obtained through blood centrifugation process, which antibacterial properties evidenced by in vitro vivo studies conducted both the human veterinary field. This study evaluated antimicrobial effect non-activated activated leucocyte-rich PRP (L-PRP) platelet-poor (PPP) against two strains SP with pyoderma: one multidrug-resistant strain (MDR) non-MDR strain. Twenty un-sedated adult donor were enrolled for L-PRP PPP production via closed semi-automatic system use. The evaluation was performed using micro-inhibition broth method, exposing 10 L-PRP, samples, respectively. Bacterial growth CFU count at three timepoints (immediately after incubation T0, 1 h T1 2 T2). had significant all similar MDR strains. Activation appeared reduce duration L-PRP. More are necessary confirm these preliminary results.

Language: Английский

Multidrug-Resistant Commensal and Infection-Causing Staphylococcus spp. Isolated from Companion Animals in the Valencia Region DOI Creative Commons
Ana Marco-Fuertes, Clara Marín,

Concepción Gimeno-Cardona

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(2), P. 54 - 54

Published: Jan. 26, 2024

The emergence of antimicrobial resistance (AMR) and multidrug (MDR) among microorganisms to commonly used antibiotics is a growing concern in both human veterinary medicine. Companion animals play significant role the epidemiology AMR, as their population continuously increasing, posing risk disseminating particularly strains public health importance, such methicillin-resistant Staphylococcus strains. Thus, this study aimed investigate prevalence AMR MDR commensal infection-causing spp. dogs cats Valencia region. For purpose, 271 samples were taken from centers assess susceptibility against 20 antibiotics, including some most important for treatment infections, five last resort list. Of all samples, 187 recovered asymptomatic skin-diseased cats, which S. pseudintermedius (≈60%) was more prevalent dogs, while felis (≈50%) cats. In overall analysis isolates, observed tested, those crucial Furthermore, over 70% 30% respectively, exhibited MDR. This highlights significance monitoring trends companion animals. potential contribution these dissemination its genes humans, other animals, shared environment underscores necessity adopting One Health approach.

Language: Английский

Citations

6
Antimicrobial resistance and virulence profiling of Staphylococcus pseudintermedius isolated from cats, Bangladesh DOI Creative Commons
Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md. Sayedul Islam

et al.

Veterinary Quarterly, Journal Year: 2024, Volume and Issue: 44(1), P. 1 - 11

Published: March 15, 2024

Staphylococcus pseudintermedius is a significant bacterial pathogen that frequently colonizes different body sites and mucous membranes of pets. The objectives the cross-sectional study were to estimate prevalence, antimicrobial resistance pattern, detection diverse as well virulence genes S. in cats. A standard bacteriological method, species-specific gene confirmed by PCR assay. total 233 swab samples collected from 102 cats, among them 146 swabs 73 healthy 87 29 diseased Overall, prevalence cats was 12.01%, while dermatitis otitis affected 26.08% 33.33%, respectively. highest observed against penicillin (96.42%) followed streptomycin (85.71%) erythromycin (78.57%). Moreover, 89.28% isolates exhibit multi-drug (MDR) (≥ 3 classes' resistant). In addition, 17.86% harbored mecA gene; thus, classified methicillin-resistant (MRSP). Furthermore, ermA ermB 25% 10.71% isolates, 42.86% carried tetK tetL (tetracycline resistance) genes, profiling, 32.14% (sea) (seb) found positive for enterotoxin whereas, toxic shock syndrome toxin-1 (tst-1) Panton-Valentine leukocidin (pvl) detected 14.29% To our knowledge, this first report Bangladesh MDR pseudintermedius, MRSP, their profiling.

Language: Английский

Citations

5
Not just in man's best friend: A review of Staphylococcus pseudintermedius host range and human zoonosis DOI Creative Commons
Emily Roberts, Tim Nuttall,

G. Gkekas

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 174, P. 105305 - 105305

Published: May 14, 2024

Staphylococcus pseudintermedius is one species in the commensal staphylococcal population dogs. While it commonly carried on healthy companion dogs also an opportunistic pathogen associated with a range of skin, ear, wound and other infections. adapted to dogs, not restricted them, we have reviewed its host range, including increasing reports human colonisation Despite association pet S. found widely animals, covering companion, livestock free-living birds mammals. Human infections, typically immunocompromised individuals, are increasingly being recognised, part due improved diagnosis. Colonisation, infection, antimicrobial resistance, frequent multidrug among isolates represent important One Health challenges.

Language: Английский

Citations

5
Multidrug-Resistant Uropathogens in Companion Animals: A Comprehensive Study from Clinical Cases and a Genomic Analysis of a CTX-M-14-Producing Escherichia coli ST354, a Leading Cause of Urinary Tract Infections DOI

Victoria Tiemi Sorbello Sakauchi,

Barbara Ponzilacqua Silva,

Amanda Haisi

et al.

Microbial Drug Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Urinary tract infections (UTIs) are common in small animals, posing significant clinical challenges due to their recurrence and discomfort. This study investigated the bacterial causes antimicrobial resistance patterns of UTIs dogs cats presented an important Veterinary Teaching Hospital São Paulo, Brazil, largest city Latin America. Samples were collected from 31 9 via ultrasound-guided cystocentesis. Bacterial cultures performed, species identification was accomplished with matrix-assisted laser desorption ionization-time flight mass spectrometry, susceptibility testing done using Kirby-Bauer method. Escherichia coli most frequently isolated pathogen, accounting for 27.9% cases, followed by Staphylococcus pseudintermedius, Proteus mirabilis, Klebsiella pneumoniae. Ampicillin observed 70.4% enterobacteria, many E. strains exhibiting multidrug resistance. Whole-genome sequencing extended-spectrum beta-lactamase-producing uropathogenic strain a feline patient performed; it identified as ST354, leading cause worldwide humans carrying blaCTX-M-14 gene other determinants. Phylogenetic analysis indicated genetic proximity between this others Brazilian poultry environmental sources. These findings emphasize need surveillance veterinary advocate stricter antibiotic stewardship inform diagnostic therapeutic approaches within One Health perspective.

Language: Английский

Citations

0
Insights into Molecular Profiles, Resistance Patterns, and Virulence Traits of Staphylococci from Companion Dogs in Angola DOI Creative Commons
Romay Coragem da Costa, Fernando Cunha, Raquel Abreu

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 1043 - 1043

Published: April 4, 2025

Staphylococci are prevalent in dogs' microbiota, with commensal strains being able to exhibit resistance and virulence traits, complicating secondary infection management. As antimicrobial is a global threat, particularly middle-income countries like Angola, surveillance of resistant bacteria crucial. We analyzed the prevalence, resistance, profiles staphylococci from dogs Angola. Isolates were identified using VITEK® 2 Compact (bioMérieux© SA, Marcy l'Etoile, France), their genetic diversity was assessed via PCR fingerprinting. Isolates' susceptibility relevant antimicrobials determined by disk diffusion, evaluated plaque assays. The relationship between antibiotic animal-related factors also statistical analysis. as Mammaliicoccus sciuri (former Staphylococcus sciuri, 38%), xylosus (30%), equorum (13%), vitulinus vitulinus, 7%), lentus lentus, 5%), aureus (2%), spp. (5%). Of these, 86% at least one tested, 30% classified multidrug-resistant, more common females, clinical signs disease, vaccinated animals. Moreover, 93% isolates produce biofilm, 46% could lecithinase gelatinase, 23% hemolysins. Companion Angola can carry express several factors, potentially representing One Health risk.

Language: Английский

Citations

0
The impact of stray animal populations on Public Health in Naples, Italy: a preliminary study on antimicrobial resistance on the road DOI Creative Commons
Francesca Paola Nocera, S. H. Arslan,

Rossana Schena

et al.

Comparative Immunology Microbiology and Infectious Diseases, Journal Year: 2025, Volume and Issue: 120, P. 102344 - 102344

Published: April 26, 2025

Language: Английский

Citations

0
Microbiota insights into pet ownership and human health DOI Creative Commons

Sepideh Abdolghanizadeh,

Elaheh Salmeh,

Fatemeh Mirzakhani

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 171, P. 105220 - 105220

Published: March 11, 2024

Language: Английский

Citations

3
Antimicrobial Resistance and Risk Factors of Canine Bacterial Skin Infections DOI Creative Commons
Qian Wang, Siyu Chen,

Shizhen Ma

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 309 - 309

Published: March 24, 2025

Bacterial skin infections are common in dogs and often secondary to underlying conditions like allergies or ectoparasite infestations. Untreated primary causes can lead recurrent an increased risk of antimicrobial resistance, including methicillin-resistant Staphylococcus pseudintermedius (MRSP), posing a substantial clinical challenge. Here, we analyzed 896 canine bacterial infection samples collected from the China Agricultural University Veterinary Teaching Hospital between 2018 2022. Species identification was confirmed by MALDI-TOF 16S rRNA gene sequencing. Of samples, 722 (80.6%) yielded 1123 isolates, with (n = 421), Pseudomonas aeruginosa 108), Escherichia coli 73) being most prevalent. Antimicrobial susceptibility evaluated using broth microdilution method according CLSI guidelines. Notably, resistance florfenicol S. 9.1% 20.0% 2022, while ceftriaxone E. rose 30.0% 72.7% over same period. Among 305 reviewed cases, pyoderma (47.5%, 145/305) type, predominantly associated 114), followed otitis (25.6%, 78/305) primarily linked P. 24). Mixed occurred 35.4% (108/305) as frequently isolated species both single mixed infections. The multivariable logistic regression model revealed that MRSP were correlated history invasion (p <0.001) prolonged disease duration (six months less than one year: p 0.005; year longer: < 0.001). Core-genome SNP analysis showed eight infected identical strains, which dog exhibited shift gentamicin within nine days. Conversely, three distinct strains at two time points. To effectively manage chronic dogs, rigorous disinfection protocols veterinary hospitals, control duration, prevention infections, continuous monitoring antibiotic patterns essential.

Language: Английский

Citations

0
Non-traditional small companion mammals in Spain as reservoirs of antimicrobial-resistant Staphylococci DOI Creative Commons
Ana Marco-Fuertes, Clara Marín,

José Villora-Gonzalez

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 9, 2024

The increasing prevalence of antimicrobial resistance (AMR) and multidrug (MDR) in microorganisms poses a significant concern both human veterinary medicine. Non-traditional companion animals (NTCAs), particularly popular amongst households with children, play crucial role AMR epidemiology due to their rising population. Indeed, it is known that some these may act as reservoirs zoonotic pathogens thus be able spread transmit them family members, along AMR, through shared environment. It therefore imperative address this the involvement human, animal environmental health professionals. This pilot study aimed assess patterns

Language: Английский

Citations

2
Staphylococcus pseudintermedius and Pseudomonas aeruginosa Lubbock Chronic Wound Biofilm (LCWB): a suitable dual-species model for in vitro studies DOI Creative Commons
Silvia Di Lodovico, Morena Petrini, Paola Di Fermo

et al.

Microbes and Infection, Journal Year: 2024, Volume and Issue: unknown, P. 105384 - 105384

Published: June 1, 2024

Antimicrobial treatment of methicillin-resistant Staphylococcus pseudintermedius associated with canine wounds represents an important challenge. The aim this study was to create a wound infection model, Lubbock Chronic Wound Biofilm (LCWB), focus on S. pseudintermedius, drawing inspiration from the established human model involving aureus. Methicillin-resistant 115 (MRSP) and Pseudomonas aeruginosa 700 strains, isolated dog wounds, were used set up LCWB at 24, 48 72 h. LCWBs evaluated in terms volume, weight, microbial CFU/mg. spatial distribution assessed by SEM CLSM imaging. best incubation time for production volume (3.38 cm3 ± 0.13), weight (0.86 gr 0.02) CFU/mg (up 7.05 × 106 2.89 105) images showed major viable colonization h non-mixed bacteria prevalence MRSP surface P. depth wound. obtained findings demonstrate capability grow together representing suitable reproduce animal chronic vitro.

Language: Английский

Citations

1