Parasites & Vectors,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: Dec. 23, 2024
Abstract
Background
Culex
quinquefasciatus
plays
a
crucial
role
as
vector
of
West
Nile
virus
(WNV).
This
mosquito
species
is
widely
distributed
in
Cape
Verde,
being
found
all
inhabited
islands
the
archipelago.
However,
no
data
are
currently
available
on
susceptibility
local
population
to
WNV.
study
aimed
assess
competence
Cx.
mosquitoes
from
Santiago
Island,
for
WNV
and
explore
potential
impact
its
native
Wolbachia
transmission.
Methods
-infected
uninfected
female
were
exposed
lineage
1
PT6.39
strain
using
Hemotek
membrane
feeding
system.
Mosquito
samples,
including
body,
legs,
wings
saliva,
collected
at
days
7,
14
21
post-infection
(dpi)
infection
through
one-step
quantitative
real-time
PCR
(RT-qPCR).
Results
Verde
exhibited
high
tested
Also,
treated
females
without
their
significantly
higher
load
bodies
greater
dissemination
rate
7
dpi
than
wild-type
counterparts
carrying
.
Conclusions
The
poses
risk
transmission
this
seems
confer
protection
against
early
stages
viral
infection.
Additional
research
required
uncover
mechanisms
driving
Graphical
abstract
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Jan. 25, 2024
Abstract
Mosquitoes
are
primary
vectors
of
pathogens
impacting
humans,
wildlife,
and
livestock.
Among
them,
the
Asian
tiger
mosquito,
Aedes
albopictus
,
stands
out
as
an
invasive
species
with
a
global
distribution,
having
established
populations
on
every
continent
except
Antarctica.
Recent
findings
incriminate
Ae.
in
local
transmission
several
causing
human
diseases,
including
dengue,
chikungunya,
Zika
viruses
worm
parasites
Dirofilaria
.
In
Spain,
establishment
occurred
2004
it
rapidly
expanded,
currently
reaching
southern
provinces
creating
novel
epidemiological
scenarios
recently
invaded
areas.
this
study,
we
conducted
captures
from
May
to
November
2022
two
provinces,
Granada
Malaga,
situated
near
current
edge
species'
expanding
range
Spain.
The
objective
was
identify
factors
influencing
their
these
regions.
were
captured
using
BG-Sentinel
traps
baited
CO
2
BG-Lure,
miniature
CDC-UV
five
different
localities.
Our
underscore
influence
both
extrinsic
factors,
such
locality,
intrinsic
mosquito
sex,
abundance
A
higher
observed
Malaga
province
compared
localities
province.
Furthermore,
similar
numbers
mosquitoes
more
urbanized
areas
Granada,
while
lowest
counts
recorded
less
area.
These
results
another
common
area,
specifically
Culex
pipiens
Overall,
represent
first
monitoring
area
discussed
light
potential
importance
nuisance
for
humans
public
health
relevance.
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(8), P. 691 - 691
Published: Aug. 15, 2024
Mosquitoes
are
vectors
of
many
important
human
diseases.
The
prolonged
and
widespread
use
insecticides
has
led
to
the
development
mosquito
resistance
these
insecticides.
gut
microbiota
is
considered
master
host
physiology;
it
influences
biology,
disease
pathogen
transmission,
Understanding
role
mechanisms
in
insecticide
useful
for
developing
new
strategies
tackling
resistance.
We
searched
online
databases,
including
PubMed,
MEDLINE,
SciELO,
Web
Science,
Chinese
Science
Citation
Database.
all
terms,
mosquitoes,
or
any
specific
genera
species
mosquitoes.
reviewed
relationships
between
growth,
development,
survival,
reproduction,
as
well
interactions
Overall,
429
studies
were
included
this
review
after
filtering
8139
search
results.
Mosquito
show
a
complex
community
structure
with
rich
diversity,
dynamic
changes
composition
over
time
(season)
across
space
(environmental
setting),
variation
among
developmental
stages
(larval
vs.
adult).
plays
profound
roles
reproduction.
There
was
reciprocal
interaction
midgut
virus
infection
Viruses,
Journal Year:
2024,
Volume and Issue:
16(9), P. 1499 - 1499
Published: Sept. 23, 2024
Insect-specific
viruses
(ISVs)
include
that
are
restricted
to
the
infection
of
mosquitoes
and
spread
mostly
through
transovarial
transmission.
Despite
using
a
distinct
mode
transmission,
ISVs
often
phylogenetically
related
arthropod-borne
(arboviruses)
responsible
for
human
diseases
able
infect
both
vertebrates.
can
also
induce
phenomenon
called
"superinfection
exclusion",
whereby
primary
ISV
in
an
insect
inhibits
subsequent
viral
infections
insect.
This
has
sparked
interest
use
control
pathogenic
arboviruses
transmitted
by
mosquitoes.
In
particular,
insect-specific
flaviviruses
(ISFs)
have
been
shown
inhibit
vertebrate-infecting
(VIFs)
vitro
vivo.
potential
as
new
ecologically
friendly
biological
approach
arboviral
disease.
For
this
intervention
lasting
impacts
control,
it
is
imperative
ISFs
maintained
mosquito
populations
with
high
rates
vertical
Therefore,
these
strategies
will
need
optimise
transmission
order
establish
persistently
infected
lines
sustainable
arbovirus
control.
review
compares
recent
observations
determinants
understand
how
may
be
optimised
effective
Insect-specific
viruses
(ISVs)
include
that
are
restricted
to
the
infection
of
mosquitoes
and
spread
mostly
through
transovarial
transmission.
Despite
using
a
distinct
mode
transmission,
ISVs
often
closely
related
arthropod-borne
(arboviruses)
responsible
for
human
diseases
able
infect
both
vertebrates.
can
also
induce
phenomenon
called
“superinfection
exclusion”,
whereby
primary
ISV
in
an
insect
inhibits
subsequent
viral
infections
insect.
This
has
sparked
interest
use
control
pathogenic
arboviruses
transmitted
by
mosquitoes.
In
particular,
insect-specific
flaviviruses
(ISF)
have
been
shown
inhibit
vertebrate-infecting
(VIFs)
vitro
vivo.
potential
as
new
ecologically
friendly
biological
approach
arboviral
disease.
For
this
intervention
lasting
impacts
control,
it
is
imperative
ISFs
maintained
mosquito
populations
with
high
rates
vertical
Therefore,
these
strategies
will
need
optimize
transmission
order
establish
persistently
infected
lines
sustainable
arbovirus
control.
review
compares
recent
observations
determinants
understand
how
may
be
optimised
effective
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(11), P. e0314001 - e0314001
Published: Nov. 18, 2024
West
Nile
virus
(WNV)
is
a
flavivirus
naturally
circulating
between
mosquito
vectors
and
birds,
occasionally
infecting
horses
humans
causing
epidemiologically
relevant
outbreaks.
In
Spain,
the
first
big
WNV
outbreak
was
recorded
in
2020,
resulting
77
people
infected
8
fatalities,
most
of
them
southern
Spain.
Culex
perexiguus
identified
as
primary
vector
maintaining
its
enzootic
circulation
virus.
Growing
evidence
highlights
role
microbiota
key
component
determining
vectorial
capacity
mosquitoes,
largely
contributing
to
disease
epidemiology.
Here,
we
develop,
our
knowledge,
identification
composition
this
under
natural
conditions
test
for
potential
relationship
infection.
To
do
so,
collected
mosquitoes
area
Spain
during
2020
using
16S
rRNA
gene
metabarcoding
approach.
The
Cx
.
dominated
by
phylum
Proteobacteria
abundant
families
were
Burkholderiaceae
Erwiniaceae
,
including
genera
Burkholderia
Erwinia
Pantoea
genus
Wolbachia
which
use
dominate
pipiens
negatively
interact
with
according
literature,
had
low
prevalence
relative
abundance
did
not
differ
WNV-positive
WNV-negative
pools.
diversity
significantly
related
infection
status.
These
results
provide
an
endemic
Journal of Visualized Experiments,
Journal Year:
2024,
Volume and Issue:
212
Published: Oct. 4, 2024
The
global
burden
of
mosquito-transmitted
diseases,
including
malaria,
dengue,
West
Nile,
Zika,
Usutu,
and
yellow
fever,
continues
to
increase,
posing
a
significant
public
health
threat.With
the
rise
insecticide
resistance
absence
effective
vaccines,
new
strategies
are
emerging
that
focus
on
mosquito's
microbiota.Nevertheless,
majority
symbionts
remain
resistant
cultivation.Characterizing
diversity
function
bacterial
genomes
in
mosquito
specimens,
therefore,
relies
metagenomics
subsequent
assembly
binning
strategies.The
obtention
analysis
Metagenome-Assembled
Genomes
(MAGs)
from
separated
organs
can
notably
provide
key
information
about
specific
role
mosquitoassociated
microbes
ovaries
(the
reproductive
organs),
midgut
(key
for
food
digestion
immunity),
or
salivary
glands
(essential
transmission
vector-borne
diseases
as
pathogens
must
colonize
them
enter
saliva
reach
bloodstream
during
blood
meal).These
newly
reconstructed
then
pave
way
development
novel
vector
biocontrol
strategies.To
this
aim,
it
is
required
isolate
while
avoiding
cross-contamination
between
with
microorganisms
present
other
organs.Here,
we
describe
an
optimized
contamination-free
dissection
protocol
studying
microbiome
at
organ
level.
