Novel LC-MS/MS Method for Simultaneous Determination of Monoamine Neurotransmitters and Metabolites in Human Samples

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: 35(4), P. 663 - 673

Published: March 6, 2024

For the simultaneous determination of monoamine neurotransmitters (NTs) like dopamine, serotonin, noradrenaline, and epinephrine, their metabolites (metanephrine, normetanephrine, 3-methoxytyramine, vanillylmandelic acid, 3,4-dihydroxyphenylacetic homovanillic 5-hydroxyindoleacetic acid), a robust liquid chromatography method coupled with tandem mass spectrometry (LC–MS/MS) was introduced as analytical method. This proved to be accurate for measurement amounts 11 NTs in biological samples. The more efficient better than previously reported terms precision, recovery, sample requirement, extraction procedure. requires only 100 μL blood 200 urine, procedure acetonitrile precipitation, filtration, drying, reconstitution water. separation all analytes performed on an C18 column (4.6 mm × 150 1.8 μm). A 10 min gradient elution program mobile phase consisting (0.2% formic acid water) B (methanol) used. positive ionization mode used detection multiple reaction monitoring (MRM). proposed validated internal standard yielded lower limits quantification ranges 0.0182–0.0797 ng/mL 0.0553–0.2415 ng/mL, respectively, good linearity (R2) between 0.9959 0.9994. recoveries ranged from 73.37% 116.63% 80.9% 115.33% urine. metabolites, intra- interday % CV were 0.24–9.36 0.85–9.67, respectively. developed LC–MS/MS successfully trace endogenous compounds human urine

Language: Английский

---

Quantification of Plasma Dopamine in Depressed Patients Using Silver-Enriched Silicon Nanowires as SERS-Active Substrates

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(2), P. 870 - 882

Published: Feb. 14, 2024

A decrease in the levels of dopamine (DA)─a key catecholamine biomarker for major depressive disorder─highlights need quantitative analysis biological fluids to aid early diagnosis diverse neuropsychiatric disorders. This study developed silicon nanowires enriched with silver nanoparticles serve as a surface-enhanced Raman scattering (SERS) substrate enable precise and sensitive quantification blood plasma DA humans. The silver-enriched (SiNWs@Ag) yielded flower-like assemblies densely populated SERS "hot spots," allowing detection. By correlating concentration intensity at 1156 cm–1, treatment-naïve patients depression (n = 18) were 2 orders magnitude lower than those healthy controls (6.56 × 10–10 M vs 1.43 10–8 M). concentrations differed significantly between two groups (two-tailed p 5.77×10-7), highlighting distinct demarcation controls. Furthermore, SiNWs@Ag effectively differentiated norepinephrine (NE) mixtures nanomolar levels, demonstrating its selective detection capability. represents first report on human samples from individuals using an technique, emphasizing potential clinical utility evaluation

Language: Английский

---

A magnetic beads-based ligand fishing method for rapid discovery of monoterpene indoles as monoamine oxidase A inhibitors from Hunteria zeylanica

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1722, P. 464896 - 464896

Published: April 10, 2024

Language: Английский

---

Unveiling the Therapeutic Potential of Folate-Dependent One-Carbon Metabolism in Cancer and Neurodegeneration

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9339 - 9339

Published: Aug. 28, 2024

Cellular metabolism is crucial for various physiological processes, with folate-dependent one-carbon (1C) playing a pivotal role. Folate, B vitamin, key cofactor in this pathway, supporting DNA synthesis, methylation and antioxidant defenses. In dividing cells, folate facilitates nucleotide biosynthesis, ensuring genomic stability preventing carcinogenesis. Additionally, neurodevelopment, essential neural tube closure central nervous system formation. Thus, dysregulation of can contribute to pathologies such as cancer, severe birth defects, neurodegenerative diseases. Epidemiological evidence highlights folate's impact on disease risk its potential therapeutic target. antifolate drugs that inhibit enzymes 1C strategies targeting receptors are current options. However, cancer complex, varying among types dietary contexts. conditions, including Alzheimer's Parkinson's diseases, deficiency exacerbates cognitive decline through elevated homocysteine levels, contributing neuronal damage. Clinical trials folic acid supplementation show mixed outcomes, underscoring the complexities neuroprotective effects. This review integrates knowledge neurodegeneration, exploring molecular mechanisms, clinical implications, strategies, which provide information advancing treatments.

Language: Английский

---

Innovative integration of multi-scale residual networks and MK-MMD for enhanced feature representation in fault diagnosis

Measurement Science and Technology, Journal Year: 2024, Volume and Issue: 35(8), P. 086108 - 086108

Published: April 25, 2024

Abstract This article proposes a method based on multi-scale expansion of residual neural networks (ResNets) to address challenges in the operation rotating components, such as bearings and gears, under complex conditions where they are often affected by environmental noise. interference leads weaker fault characteristics, making feature selection difficult increasing presence extraneous information features. To tackle this issue, proposed first employs ResNet extract features from vibration signals machinery. The decomposes signal into multiple sub-signals different scales, extracting local at each scale. It then uses connections combine these obtain global representation. Furthermore, introduces construction maximum mean discrepancy (MMD) minimization entropy boundaries adapt differences between two domains. utilizes kernel functions calculate distances data scales combines comprehensive measure. By employing MMD boundary approach, can more accurately determine whether belong same category, thereby improving diagnostic accuracy robustness. Experimental results demonstrate effectiveness unsupervised cross-domain diagnosis tasks. Future work will focus further optimizing architecture ResNets, enhancing extraction capabilities, exploring advanced augmentation methods improve model’s generalization performance.

Language: Английский

---

Unraveling emotional regulation through multimodal neuroimaging techniques

Brainbridge, Journal Year: 2024, Volume and Issue: 1(1), P. 1 - 26

Published: May 30, 2024

Neuroimaging techniques have significantly advanced our understanding of emotional regulation by elucidating the neural mechanisms involved. This review synthesizes findings across multiple modalities—functional Magnetic Resonance Imaging (fMRI), Electroencephalography (EEG), Magnetoencephalography (MEG), Positron Emission Tomography (PET), and Spectroscopy (MRS)—to highlight key insights into processing. The prefrontal cortex (PFC), amygdala, hippocampus, insula emerge as critical brain regions in regulating emotions. fMRI studies demonstrate increased PFC activity decreased amygdala responses during effective emotion regulation, indicating top-down control mechanisms. EEG MEG provide temporal dynamics responses, capturing rapid changes tasks. PET MRS reveal neurochemical basis emphasizing roles neurotransmitters like serotonin dopamine. Integration multimodal approaches, such fMRI-EEG fMRI-PET, enhances combining spatial, temporal, specificity. Challenges include methodological limitations need for diverse participant samples to improve generalizability. Future research should focus on improving spatial resolution, adopting longitudinal ecologically valid designs, fostering interdisciplinary collaborations. These advancements hold promise developing personalized interventions treatments disorders leveraging neuroimaging biomarkers underpinnings regulation.

Language: Английский

---

A Comparison of Phenolic, Flavonoid, and Amino Acid Compositions and In Vitro Antioxidant and Neuroprotective Activities in Thai Plant Protein Extracts

Molecules, Journal Year: 2024, Volume and Issue: 29(13), P. 2990 - 2990

Published: June 23, 2024

The leaves of mulberry, Azolla spp., sunflower sprouts, cashew nut, and mung bean are considered rich sources plant protein with high levels branched-chain amino acids. Furthermore, they contain beneficial phytochemicals such as antioxidants anti-inflammatory agents. Additionally, there reports suggesting that an adequate consumption acids can reduce nerve cell damage, delay the onset memory impairment, improve sleep quality. In this study, isolates were prepared from bean. acid profile, dietary fiber content, phenolic flavonoid content evaluated. Pharmacological properties, antioxidant, anticholinesterase, monoamine oxidase, γ-aminobutyric transaminase (GABA-T) activities, also assessed. This study found concentrated beans has a higher quantity essential (52,161 mg/100 g protein) compared to sprouts (47,386 protein), spp. (42,097 nut (26,710 mulberry (8931 protein). ranged 0.90% 3.24%, while 0.25 2.29 mg/g 0.01 2.01 sample, respectively. Sunflower sprout exhibited highest (3.24%), (2.292 ± 0.082 mg GAE/g), flavonoids (2.014 quercetin/g sample). biological efficacy evaluation extract antioxidant activity; percentages inhibition 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) 20.503 0.288% 18.496 0.105%, Five plant-based proteins potent acetylcholinesterase (AChE) enzyme activity, oxidase (MAO) inhibition, GABA-T ranging 3.42% 24.62%, 6.14% 20.16%, 2.03% 21.99%, These findings suggest these extracts be used natural resources for developing food supplements neuroprotective activity.

Language: Английский

---

Monoamine oxidase and neurodegeneration: mechanisms, inhibitors and natural compounds for therapeutic intervention.

Neurochemistry International, Journal Year: 2024, Volume and Issue: 179, P. 105831 - 105831

Published: Aug. 14, 2024

Language: Английский

---

Exploring New Mechanism of Depression from the Effects of Virus on Nerve Cells

Cells, Journal Year: 2023, Volume and Issue: 12(13), P. 1767 - 1767

Published: July 3, 2023

Depression is a common neuropsychiatric disorder with long-term recurrent depressed mood, pain and despair, pessimism anxiety, even suicidal tendencies as the main symptoms. usually induces or aggravates development of other related diseases, such sleep disorders endocrine disorders. In today's society, incidence depression increasing worldwide, its pathogenesis complex generally believed to be genetic, psychological, environmental, biological factors. Current studies have shown key role glial cells in depression, it noteworthy that some recent evidence suggests may closely viral infections, SARS-CoV-2, BoDV-1, ZIKV, HIV, HHV6, which infect organism cause degree cells, astrocytes, oligodendrocytes, microglia. This can affect transmission proteins, neurotransmitters, cytokines, turn leads neuroinflammation depression. Based on close relationship between viruses this paper provides an in-depth analysis new mechanism virus-induced expected provide perspective idea for diagnosis future.

Language: Английский

---

Preparation of branched polyethyleneimine‐assisted boronic acid‐functionalized magnetic MXene for the enrichment of catecholamines in urine samples

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 47(1)

Published: Dec. 8, 2023

Herein, a magnetic borate‐functionalized MXene composite with multiple boronic affinity sites was fabricated by embedding Fe 3 O 4 nanoparticles 4‐formylphenylboronic acid functionalized Ti C 2 T x nanosheets and served as sorbent for the simultaneous extraction of catecholamines (CAs) in urine samples. The morphology structure materials were investigated using scanning microscopy, vibrating sample magnetometer, X‐ray photoelectron spectrometer, diffraction. introduction polyethyleneimine can amplify bonded groups, thereby effectively improving adsorption capacities CAs based on interactions affinity, hydrogen bonding, metal coordination. performance kinetics isotherms models, main parameters that influence efficiency optimized. Under most favorable solid‐phase condition, sensitive method analysis samples developed combining conditions high‐performance liquid chromatography detection. findings illustrated proposed approach possessed wide linearity range 0.05–250 ng/mL an acceptable correlation coefficient (R ≥ 0.9984) detection limits 0.010–0.015 target CAs. research not only provides notable but also offers effective feasible measure biological

Language: Английский

---