Nutrients,
Journal Year:
2024,
Volume and Issue:
16(24), P. 4298 - 4298
Published: Dec. 12, 2024
Background:
Intestinal
aging
is
characterized
by
declining
protein
homeostasis
via
reduced
proteasome
activity,
which
are
hallmarks
of
age-related
diseases.
Our
previous
study
showed
that
caffeine
intake
improved
intestinal
integrity
with
age
reducing
vitellogenin
(VIT,
yolk
protein)
in
C.
elegans.
In
this
study,
we
investigated
the
regulatory
mechanisms
improves
and
reduces
(VIT)
production
aged
Caenorhabditis
Methods:
We
performed
RNA-seq
analysis,
qRT-PCR
to
validate
confirm
results.
Transgenic
worms
VIT-2::GFP
VIT-6::GFP
were
used
for
measuring
VIT
production.
dsRNAi
was
conducted
elucidate
roles
pas-1
pas-3
genes.
Results:
pas-3,
a
elegans
ortholog
human
PASM4,
upregulated
intake.
They
repressing
unc-62,
transcriptional
activator
vit
expression.
Interestingly,
vit-2
required
expression,
RNAi
promoted
atrophy
colonization,
suggesting
balancing
mechanism
levels
health.
Additionally,
lifespan
extended
(2
±
0.05
days),
however,
effect
not
observed
but
RNAi,
mode
action
an
anti-aging
through
distinctive.
The
mediated
SKN-1
activation.
Conclusions:
Caffeine
enhances
health
activity
extends
upregulating
pas-3.
These
findings
suggest
consumption
mitigates
impairment
maintains
during
aging.
Frontiers in Genetics,
Journal Year:
2024,
Volume and Issue:
15
Published: Feb. 13, 2024
Background:
Physical
weakness
and
cardiovascular
risk
increase
significantly
with
age,
but
the
underlying
biological
mechanisms
remain
largely
unknown.
This
study
aims
to
reveal
causal
effect
of
circulating
metabolites
on
frailty,
sarcopenia
vascular
aging
related
traits
diseases
through
a
two-sample
Mendelian
Randomization
(MR)
analysis.
Methods:
Exposures
were
486
analyzed
in
genome-wide
association
(GWAS),
while
outcomes
included
sarcopenia,
arterial
stiffness,
atherosclerosis,
peripheral
disease
(PAD)
aortic
aneurysm.
Primary
estimates
calculated
using
inverse-variance
weighted
(IVW)
method.
Methods
including
MR
Egger,
median,
Q-test,
leave-one-out
analysis
used
for
sensitive
Results:
A
total
125
suggestive
causative
associations
between
identified.
Seven
strong
links
ultimately
identified
six
(kynurenine,
pentadecanoate
(15:0),
1-arachidonoylglycerophosphocholine,
androsterone
sulfate,
glycine
mannose)
three
(sarcopenia,
PAD
atherosclerosis).
Besides,
metabolic
pathway
13
significant
pathways
6
age-related
diseases.
Furthermore,
metabolite-gene
interaction
networks
constructed.
Conclusion:
Our
research
suggested
new
evidence
relationship
diseases,
which
may
hold
promise
as
valuable
biomarkers.
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(11), P. e0309795 - e0309795
Published: Nov. 14, 2024
Background
The
study’s
primary
goal
is
to
ascertain
whether
there
a
relationship
between
the
processed
green
tea
methanolic
extract’s
(GTME)
phytochemical
components
and
its
potential
effectiveness
against
human
liver
cancer
cells.
GTME’s
composition
was
identified
using
gas
chromatography-mass
spectrometry,
capacity
lower
cellular
proliferation
cause
apoptosis
in
HepG2
cancerous
cell
lines
checked.
Results
findings
of
chromatogram
showed
that
GTME
included
bioactive
antioxidants
anticancer
substances.
Additionally,
utilizing
MTT,
comet
assay,
acridine
revealed
selective
cytotoxic
impact
with
significant
IC
50
value
(27.3
µg/ml)
on
cells
without
any
harmful
effects
WI-38
healthy
Also,
compared
untreated
cells,
extract-treated
had
an
upsurge
proportion
have
undergone
displayed
nucleus,
which
sign
DNA
damage.
In
addition,
treated
stop
G1
phase
sub-G1
apoptotic
(37.32%)
flow
cytometry
analysis.
Furthermore,
reactive
oxygen
species
were
shown
be
responsible
for
apoptosis,
tested
extract
significantly
reduced
their
levels
Lastly,
changed
protein
expression
linked
cycle
arrest
apoptosis.
Conclusion
These
provided
information
about
processes
through
inhibited
growth
HepG2.
Therefore,
it
has
as
effective
natural
therapy
treatment
cancer.
However,
validate
these
findings,
animal
models
must
used
vivo
studies.
ChemMedChem,
Journal Year:
2024,
Volume and Issue:
19(15)
Published: April 26, 2024
Abstract
Xanthines
are
purine
derivatives
predominantly
found
in
plants.
These
include
compounds
such
as
caffeine,
theophylline,
and
theobromine
exhibit
a
variety
of
pharmacological
properties,
demonstrating
efficacy
treating
neurodegenerative
disorders,
respiratory
dysfunctions,
also
cancer.
The
versatile
attributes
these
materials
render
them
privileged
scaffolds
for
the
development
various
biological
applications.
N‐heterocyclic
carbene
precursors
that
combine
pyrimidine
an
imidazole
ring.
Owing
to
their
relevance,
xanthines
have
been
employed
carbenes
metallodrugs
anticancer
antimicrobial
purposes.
In
this
conceptual
review,
we
examine
key
examples
complexes
derived
from
caffeine
other
xanthines,
elucidating
synthetic
methods
describing
pertinent
medicinal
