Advances in pharmaceutical and biomedical applications of plant–based nano-biopolymers with special emphasis on vaginal drug delivery systems: A review

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120592 - 120592

Published: Jan. 25, 2025

Language: Английский

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Kombucha Versus Vegetal Cellulose for Affordable Mucoadhesive (nano)Formulations

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 37 - 37

Published: Jan. 4, 2025

Cellulose nanofibers gained increasing interest in the production of medical devices such as mucoadhesive nanohydrogels due to their ability retain moisture (high hydrophilicity), flexibility, superior porosity and durability, biodegradability, non-toxicity, biocompatibility. In this work, we aimed compare suitability selected bacterial vegetal nanocellulose form hydrogels for biomedical applications. The cellulose were synthesized from brewer's spent grains (BSG) kombucha membranes, respectively. Two prepared, one based on other (VNC BNC, respectively). VNC was less opaque more fluid than BNC. cytocompatibility vitro antioxidant activity nanocellulose-based investigated using human gingival fibroblasts (HGF-1, ATCC CRL-2014). investigation hydrogel-mucin interaction revealed that BNC hydrogel had an approx. 2× higher mucin binding efficiency at a hydrogel/mucin ratio (mg/mg) = 4. exhibited highest potential increase number metabolically active viable cells (107.60 ± 0.98% cytotoxicity negative control) among all culture conditions. reduced amount reactive oxygen species (ROS) by about 23% (105.5 2.2% C-) comparison with positive control, whereas ROS level slightly (120.2 3.9% following treatment. Neither two showed antibacterial when assessed diffusion method. data suggest fermentation could be better candidate cytocompatible nanoformulations grains. both VNC, respectively, should improved.

Language: Английский

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Current Paradigms and Future Challenges in Harnessing Nanocellulose for Advanced Applications in Tissue Engineering: A Critical State-of-the-Art Review for Biomedicine

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1449 - 1449

Published: Feb. 9, 2025

Nanocellulose-based biomaterials are at the forefront of biomedicine, presenting innovative solutions to longstanding challenges in tissue engineering and wound repair. These advanced materials demonstrate enhanced mechanical properties improved biocompatibility while allowing for precise tuning drug release profiles. Recent progress design, fabrication, characterization these underscores their transformative potential biomedicine. Researchers employing strategic methodologies investigate characterize structure functionality nanocellulose In engineering, nanocellulose-based scaffolds offer opportunities replicate complexities native tissues, facilitating study effects on metabolism, vascularization, cellular behavior engineered liver, adipose, tumor models. Concurrently, has gained recognition as an dressing material, leveraging its ability deliver therapeutic agents via topical, transdermal, systemic pathways simultaneously promoting proliferation regeneration. The inherent transparency provides a unique advantage, enabling real-time monitoring healing progress. Despite advancements, significant remain large-scale production, reproducibility, commercial viability biomaterials. This review not only hurdles but also outlines directions future research, including need bioengineering dressings with scalable production incorporation novel functionalities clinical translation. By addressing key challenges, redefine biomedical material design unmet needs beyond.

Language: Английский

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The Potential of Hydrogel Preparations Containing Plant Materials in Supporting the Treatment of Vaginal and Vulvar Infections—Current State of Knowledge

Polymers, Journal Year: 2025, Volume and Issue: 17(4), P. 470 - 470

Published: Feb. 11, 2025

Vaginal hydrogels are a modern alternative to solid (tablets, globules) and other semi-solid forms of medication (ointments, creams) in the control pathogenic microorganisms diseases female reproductive tract. This review aims summarize current state knowledge regarding efficacy containing plant materials treatment vaginal vulvar infections. New therapies essential address growing antimicrobial resistance crisis. Google Scholar, Web Science, Cochrane, Medline (PubMed) databases were searched. Twenty-five studies included review, including basic, pre-clinical, clinical studies. The results obtained confirmed therapeutic potential raw embedded polymer matrix hydrogels. However, due small number trials conducted, further research this area is needed.

Language: Английский

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Cytocompatibility, Antimicrobial and Antioxidant Activity of a Mucoadhesive Biopolymeric Hydrogel Embedding Selenium Nanoparticles Phytosynthesized by Sea Buckthorn Leaf Extract

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 17(1), P. 23 - 23

Published: Dec. 22, 2023

Phytosynthesized selenium nanoparticles (SeNPs) are less toxic than the inorganic salts of and show high antioxidant antibacterial activity. Chitosan prevents microbial biofilm formation can also determine dispersal. Never-dried bacterial nanocellulose (NDBNC) is an efficient carrier bioactive compounds a flexible nanofibrillar hydrophilic biopolymer. This study aimed to develop selenium-enriched hydrogel nanoformulation (Se-HNF) based on NDBNC from kombucha fermentation fungal chitosan with embedded biogenic SeNPs phytosynthesized by aqueous extract sea buckthorn leaves (SbLEx)-SeNPsSb-in order both disperse gingival dysbiotic prevent its development. We determined total phenolic content activity SbLEx. Liquid chromatography-mass spectrometry (LC-MS) high-performance liquid chromatography (HPLC) were used for identification polyphenols SeNPsSb characterized transmission electron microscopy-energy-dispersive X-ray spectroscopy (TEM-EDX), dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FTIR) diffraction (XRD) in small- wide-angle (SAXS WAXS). The was SEM, FTIR, XRD, rheology, mucin binding efficiency, contact angle interfacial tension measurements. assessed vitro biocompatibility, antimicrobial antibiofilm potential Se-HNF. TEM, DLS SAXS evidenced polydisperse SeNPsSb, whereas FTIR highlighted heterogeneous biocorona various biocompounds. polar surface smaller (52.82 ± 1.23°) that obtained non-polar (73.85 0.39°). 97.6 0.47 mN/m. efficiency Se-HNF decreased as amount decreased, SEM analysis showed relatively compact structure upon contact. XRD analyses interaction between BNC CS through characteristic peak shifting, rheological measurements pseudoplastic behavior, 0.186 N adhesion force 0.386 energy. results degree cytocompatibility significant

Language: Английский

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PLCL/SF/NGF nerve conduit loaded with RGD‐TA‐PPY hydrogel promotes regeneration of sciatic nerve defects in rats through PI3K/AKT signalling pathways

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(15)

Published: Aug. 1, 2024

Abstract Peripheral nerve defect are common clinical problem caused by trauma or other diseases, often leading to the loss of sensory and motor function in patients. Autologous transplantation has been gold standard for repairing peripheral defects, but its application is limited due insufficient donor tissue. In recent years, tissue engineering methods synthesize conduits treating become a current research focus. This study introduces novel approach defects using tissue‐engineered PLCL/SF/NGF@TA‐PPy‐RGD conduit. The conduit was fabricated combining electrospun PLCL/SF with an NGF‐loaded conductive TA‐PPy‐RGD gel. gel, synthesized from RGD‐modified tannic acid (TA) polypyrrole (PPy), provides growth anchor points cells. vitro results showed that this hybrid could enhance PC12 cell proliferation, migration, reduce apoptosis under oxidative stress. Furthermore, activated PI3K/AKT signalling pathway rat model sciatic defect, significantly improved function, gastrocnemius muscle myelin sheath axon thickness, comparable autologous transplantation. It also promoted angiogenesis around defect. suggests provide conducive environment regeneration, offering new strategy treatment, provided theoretical basis strategies treatment

Language: Английский

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Bioactive Hydrogel Formulation Based on Ferulic Acid-Grafted Nano-Chitosan and Bacterial Nanocellulose Enriched with Selenium Nanoparticles from Kombucha Fermentation

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(7), P. 202 - 202

Published: July 22, 2024

Selenium nanoparticles (SeNPs) have specific properties that result from their biosynthesis particularities. Chitosan can prevent pathogenic biofilm development. A wide palette of bacterial nanocellulose (BNC) biological and physical-chemical are known. The aim this study was to develop a hydrogel formulation (SeBNCSFa) based on ferulic acid-grafted chitosan enriched with SeNPs Kombucha fermentation (SeNPsK), which could be used as an adjuvant for oral implant integration other applications. grafted SeBNCSFa were characterized by biochemical methods. cell viability proliferation HGF-1 gingival fibroblasts investigated, well in vitro antioxidant activity. inflammatory response determined enzyme-linked immunosorbent assay (ELISA) the proinflammatory mediators (IL-6, TNF-α, IL-1β) culture medium. Likewise, amount nitric oxide released measured Griess reaction. antimicrobial activity also investigated. grafting degree acid approximately 1.780 ± 0.07% total monomeric units, assuming single-site per monomer. Fourier-transform infrared spectroscopy evidenced convolution BNC spectra, X-ray diffraction analysis highlighted amorphous rearrangement patterns, suggesting multiple interactions. showed high cytocompatibility, enhanced antioxidant, anti-inflammatory, potentials.

Language: Английский

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