Petanin Potentiated JNK Phosphorylation to Negatively Regulate the ERK/CREB/MITF Signaling Pathway for Anti-Melanogenesis in Zebrafish

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5939 - 5939

Published: May 29, 2024

Petanin, an acylated anthocyanin from the Solanaceae family, shows potential in tyrosinase inhibitory activity and anti-melanogenic effects; however, its mechanism remains unclear. Therefore, to investigate underlying of petanin’s effects, enzyme activity, protein expression mRNA transcription melanogenic related signaling pathways zebrafish using network pharmacology, molecular docking dynamics simulation were combined for analysis. The results showed that petanin could inhibit melanogenesis, change distribution arrangement melanocytes structure melanosomes, reduce activities catalase (CAT) peroxidase (POD) enhance glutathione reductase (GR). It also up-regulated JNK phosphorylation, inhibited ERK/RSK phosphorylation down-regulated CREB/MITF-related transcription. These consistent with predictions provided through pharmacology docking. Thus, by inhibiting negatively regulating tyrosinase-related pathway ERK/CREB/MITF p-JNK. In conclusion, is a good inhibitor anti-melanin natural compound significant market prospects melanogenesis-related diseases skin whitening cosmetics.

Language: Английский

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Machine Learning-Based Process Optimization in Biopolymer Manufacturing: A Review

Polymers, Journal Year: 2024, Volume and Issue: 16(23), P. 3368 - 3368

Published: Nov. 29, 2024

The integration of machine learning (ML) into material manufacturing has driven advancements in optimizing biopolymer production processes. ML techniques, applied across various stages production, enable the analysis complex data generated throughout identifying patterns and insights not easily observed through traditional methods. As sustainable alternatives to petrochemical-based plastics, biopolymers present unique challenges due their reliance on variable bio-based feedstocks processing conditions. This review systematically summarizes current applications techniques aiming provide a comprehensive reference for future research while highlighting potential enhance efficiency, reduce costs, improve product quality. also shows role algorithms, including supervised, unsupervised, deep

Language: Английский

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Mechanistic Insights into the Stimulatory Effect of Melanogenesis of 4-Methylcoumarin Derivatives in B16F10 Melanoma Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12421 - 12421

Published: Nov. 19, 2024

Vitiligo is a skin condition characterized by the loss of pigment, resulting in white patches on various parts body. It occurs when melanocytes, cells that are responsible for producing destroyed or stop functioning. This study aimed to investigate melanogenic potential 4-methylcoumarin (4MC) derivatives, including 6-methoxy-4-methylcoumarin (6M-4MC), 7-methoxy-4-methylcoumarin (7M-4MC), 7-amino-4-methylcoumarin (7A-4MC), 6,7-dihydroxy-4-methylcoumarin (6,7DH-4MC), 7,8-dihydroxy-4-methylcoumarin (7,8DH-4MC), and 6,7-dimethoxy-4-methylcoumarin (6,7DM-4MC), B16F10 melanoma cells. Our findings revealed that, while 4MC, 7A-4MC, 6,7DH-4MC, 7,8DH-4MC did not exhibit any effect melanin production, significant stimulation melanogenesis was observed with 6M-4MC, 7M-4MC, 6,7DM-4MC, 6M-4MC demonstrating most pronounced effect. significantly stimulated production tyrosinase activity concentration-dependent manner A Western blot analysis increased expression levels microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), protein-2 (TRP-2). Further mechanistic studies showed inhibited extracellular signal-regulated kinase (ERK) protein B (AKT), which led upregulation MITF TRP proteins subsequent activation synthesis. Additionally, activated GSK3β phosphorylation, reduced β-catenin via GSK3β/β-catenin pathway. Moreover, primary irritation test conducted upper backs 32 healthy female volunteers assess sensitization from applied topically at concentrations 50 µM 100 µM. The results no adverse effects skin. Collectively, these suggest may be promising pigmentation stimulator use cosmetics medical treatment hypopigmentation disorders, particularly conditions such as vitiligo.

Language: Английский

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Enhancing Radiation Shielding Efficiency of Nigella sativa Eumelanin Polymer Through Heavy Metals Doping

Polymers, Journal Year: 2025, Volume and Issue: 17(5), P. 609 - 609

Published: Feb. 25, 2025

Gamma radiation shielding is necessary for many applications; nevertheless, lead creates environmental risks. Eumelanin, a natural polymer, viable alternative, although its effectiveness limited to lower gamma-ray energy. This research looks at how doping the herbal eumelanin polymer (Nigella sativa) with heavy metals including iron (Fe), copper (Cu), and zinc (Zn) affects gamma characteristics. The inclusion of these considerably increases linear attenuation coefficient (μ) mass (μm) eumelanin, especially photon energies where photoelectric effect prominent. μ value pure 0.193 cm-1 59.5 keV. It goes up 0.309 cm-1, 0.420 0.393 when Fe, Cu, Zn are added, in that order. Similarly, coefficients increase from 0.153 cm2/g 0.230, 0.316, 0.302 Fe-, Cu-, Zn-doped samples. At intermediate higher (661.7 keV-to-1332.5 keV), Compton scattering main interaction, differences between samples not as noticeable, which means metal additions have less an effect. mean free path (MFP) protection efficiency (RPE) also show behaviors. For example, keV MFP drops 5.172 cm 3.244 Mel-Fe, 2.385 Mel-Cu, 2.540 Mel-Zn. RPE values go lot low energies. Cu-doped has highest 34.251%, while only 17.581%. However, all converge, suggesting more consistent performance. These findings suggest particularly effective enhancing energies, (Cu) providing most significant improvement overall, making composites suitable applications requiring enhanced

Language: Английский

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Umckalin Promotes Melanogenesis in B16F10 Cells Through the Activation of Wnt/β-Catenin and MAPK Signaling Pathways

Applied Biosciences, Journal Year: 2025, Volume and Issue: 4(2), P. 20 - 20

Published: April 2, 2025

Melanogenesis is regulated by melanogenic enzymes such as tyrosinase (TYR), TRP-1, and TRP-2, whose expression controlled the microphthalmia-associated transcription factor (MITF). Various signaling pathways, including cAMP/PKA, MAPK/ERK, Wnt/β-catenin, PI3K/Akt, are involved in this process have been a focal point of research for treating pigmentation disorders. However, developing effective therapies conditions like vitiligo remains significant challenge. In study, effects umckalin on melanogenesis its molecular mechanisms were investigated using B16F10 cells, mouse melanoma cell line widely used model melanin production studies. cells produce via melanosomes express key TYR, making them reliable system. Our findings demonstrate that promotes concentration-dependent manner upregulating TRP-1 activating MITF pathway. Additionally, modulated GSK3β/β-catenin MAPK, to enhance melanogenesis. conclusion, enhances enzyme activity critical thereby promoting synthesis. These suggest could be promising candidate therapeutic agents disorders vitiligo. Further studies required explore clinical applications greater detail.

Language: Английский

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Rifampicin Repurposing Reveals Anti-Melanogenic Activity in B16F10 Melanoma Cells

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 900 - 900

Published: Feb. 15, 2025

Drug repurposing is a cost-effective and innovative strategy for identifying new therapeutic applications existing drugs, thereby shortening development timelines accelerating the availability of treatments. Applying this approach to cosmeceutical ingredients enables creation functional compounds with proven safety efficacy, adding significant value cosmetic industry. This study evaluated potential rifampicin, drug widely used treatment tuberculosis leprosy, as agent. The anti-melanogenic effects rifampicin were assessed in B16F10 melanoma cells, showing no cytotoxicity at concentrations up 40 µM reduction intracellular tyrosinase activity melanin content. Mechanistically, reduced expression melanogenic enzymes, including tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2, via kinase A (PKA)-dependent pathway, leading suppression microphthalmia-associated transcription factor (MITF), which key regulator melanogenesis. Additionally, inhibited p38 signaling pathway but was independent PI3K/protein B (Akt) pathway. Furthermore, it decreased Ser9 phosphorylation, enhancing glycogen synthase kinase-3β (GSK-3β) activity, promoted β-catenin facilitated degradation, collectively contributing inhibition synthesis. To evaluate topical applicability primary human skin irritation tests conducted, adverse observed 20 µM. These findings demonstrate that inhibits melanogenesis through multiple pathways, PKA, MAPKs, GSK-3β/β-catenin. highlights be repurposed agent managing hyperpigmentation disorders, offering valuable insights into novel strategies pigmentation-related conditions.

Language: Английский

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Design and Synthesis of Novel 6-(Substituted phenyl)-[1,3]dioxolo[4′,5′:4,5]benzo[1,2-d]thiazole Compounds as Tyrosinase Inhibitors: In Vitro and In Vivo Insights

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1535 - 1535

Published: March 30, 2025

The 2,4-dihydroxyphenyl group is commonly present in the chemical structures of potent tyrosinase inhibitors. Based on this observation, a series 6-(substituted phenyl)-[1,3]dioxolo[4′,5′:4,5]benzo[1,2-d]thiazole compounds 1–13 were designed and synthesized as potential Among these, 5 9 strongly inhibited mushroom activity. Particularly, compound exhibited nanomolar IC50 values regardless substrate used, whereas kojic acid yielded 15.99–26.18 μM. Kinetic studies revealed that competitively activity, findings further corroborated by silico docking analysis. In B16F10 cell-based experiments, both effectively cellular activity melanin formation. These inhibitory effects confirmed through situ assays. Compound strong antioxidant scavenging radicals, suggesting its ability to reduce production may be attributed combination properties. Additionally, five compounds, including 5, demonstrated effective depigmentation vivo zebrafish embryos, their efficacy was similar acid, even at concentrations hundreds times lower. suggest promising anti-melanogenic agents.

Language: Английский

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Ionising and ultraviolet radiation induce the secretion of melanin in Tetrahymena pyriformis

International Journal of Environmental Studies, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Oct. 10, 2024

The objective of this study was to determine the effect ionising and ultraviolet radiation on stimulating melanin secretion in Tetrahymena pyriformis. Cell cultures were exposed different doses for 48, 72 96 h. After incubation, extracted analysed. Our results showed that concentration increased with increasing irradiation dose exposure duration. Melanin insoluble water organic solvents but soluble alkaline acidic solutions. A linear correlation between logarithm absorbance wavelength ranging from 400 600 nm observed. HPLC chromatograms degradation products by hydrogen peroxide oxidation method revealed existence four peaks. natural standard cuttlefish Sepia officinalis used as a reference compound eumelanin markers. These markers detected control cells, irradiated cells black human hair intensities.

Language: Английский

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Carnosic acid ameliorates postinflammatory hyperpigmentation by inhibiting inflammatory reaction and melanin deposition

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117522 - 117522

Published: Oct. 10, 2024

Language: Английский

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Physiological Roles of Eumelanin- and Melanogenesis-Associated Diseases: A Look at the Potentialities of Engineered and Microbial Eumelanin in Clinical Practice

Bioengineering, Journal Year: 2024, Volume and Issue: 11(8), P. 756 - 756

Published: July 25, 2024

This paper aims to highlight the physiological actions exerted by eumelanin present in several organs/tissues of human body and rationalise often conflicting functional roles played this biopolymer on basis its peculiar properties. Besides pigmentary disorders, a growing number organ injuries degenerative pathologies are presently ascribed modification levels terms alterations chemical/structural features, partial loss or uneven distribution pigment. The review analyses more recent research dedicated pathological provides an insight into some melanogenesis-associated diseases skin, eye, ear, brain, including most significant neurodegenerative disorders. Also described potentialities therapies based localised supply exogeneous EU opportunities that produced via synthetic biology offers order redesign therapeutical diagnostic applications.

Language: Английский

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