Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Near-infrared photoimmunotherapy (NIR-PIT) is a cancer treatment modality that uses antibody-photoabsorber (IR700) conjugates to destroy specific cells. The reaction between the antibody and photoabsorber triggered by NIR-light, this alters shape hydrophilicity of conjugate. This photochemical responsible for NIR-PIT-induced cell death; however, detailed mechanism underlying effect remains unknown. In study, we demonstrated actin filaments underneath membrane play an important role in death IR700 mediates conjugates, leading filament aggregation upon NIR-light irradiation. destruction cortical beneath plasma allows water flow into based on osmotic conditions, resulting rupture. sequence events may constitute death, making NIR-PIT promising modality.

Language: Английский

Contrast-enhanced ultrasound imaging for monitoring the efficacy of near-infrared photoimmunotherapy

EBioMedicine, Journal Year: 2023, Volume and Issue: 95, P. 104737 - 104737

Published: Aug. 7, 2023

Near-infrared photoimmunotherapy (NIR-PIT) is a promising cancer therapy combining NIR-light irradiation with an antibody and IR700DX, light-sensitive substance, to destroy tumours. However, homogeneous difficult because the light varies depending on distance tissue environment. Therefore, markers that indicate sufficient are necessary. Nanoparticles sized 10∼200 nm show enhanced permeation retention within tumours, which further via NIR-PIT (super permeability retention, SUPR). We aimed monitor effectiveness of by measuring SUPR.A xenograft mouse tumour model was established inoculating human cells in both buttocks Balb/C-nu/nu mice, performed only one side. To evaluate SUPR, fluorescent signal examination using QD800-fluorescent nanoparticles NIR-fluorescent poly (d,l-lactide-co-glycolic acid) (NIR-PLGA) microparticles. Harmonic signals were evaluated micro-bubbles contrast agent Sonazoid contrast-enhanced ultrasound (CEUS) imaging. The correlation between SUPR immediately after treatment day evaluated.QD800 persisted treated intensity remaining showed high positive therapeutic effect. NIR-PLGA Sonazoid-derived harmonic remained for longer time tumours than controls, kE value two-compartment correlated effectiveness.SUPR measurement CEUS imaging could be easily adapted clinical use as image-based biomarker monitoring confirming efficacy.This research supported ARIM JAPAN MEXT, Program Developing Next-generation Researchers (Japan Science Technology Agency), KAKEN (18K15923, 21K07217) (JSPS), CREST (JPMJCR19H2, JST), FOREST-Souhatsu (JST). Mochida Memorial Foundation Medical Pharmaceutical Research; Takeda Foundation; Japan Health Princess Takamatsu Cancer Research Fund. Funders provided financial support had no role study design, data collection, analysis, interpretation, writing report.

Language: Английский

近红外光免疫治疗策略靶向肿瘤微环境的研究进展

Chinese Journal of Lasers, Journal Year: 2024, Volume and Issue: 51(9), P. 0907010 - 0907010

Published: Jan. 1, 2024

近红外光免疫治疗(NIR-PIT)是一种结合抗体和光吸收剂IRDye700DX的新型肿瘤疗法,它既能够激活局部免疫效应,又能够增强肿瘤靶向性,已在不同肿瘤类型的治疗中显示出巨大的应用潜力。大量研究已经证实肿瘤微环境是导致肿瘤不断发展的重要原因,因此NIR-PIT中的光免疫偶联物靶点也已经扩展至肿瘤微环境中非肿瘤细胞的表面蛋白中。利用NIR-PIT局部消除肿瘤微环境中某些具有特定标志物的免疫抑制细胞、血管或肿瘤成纤维细胞,将解除免疫抑制,最大效率发挥机体的正常免疫功能,取得最佳的疗效。主要综述了NIR-PIT的治疗策略和靶向肿瘤微环境的最新研究进展。