Agriculture and environmental management through nanotechnology: Eco-friendly nanomaterial synthesis for soil-plant systems, food safety, and sustainability

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 171862 - 171862

Published: March 23, 2024

Language: Английский

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Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Plants, Journal Year: 2022, Volume and Issue: 11(14), P. 1856 - 1856

Published: July 15, 2022

Anthracnose is a severe disease caused by Colletotrichum spp. on several crop species. Fungal infections can occur both in the field and at post-harvest stage causing lesions fruits economic losses. Physical treatments synthetic fungicides have traditionally been preferred means to control anthracnose adverse effects; however, urgent need decrease use of toxic chemicals led investigation innovative sustainable protection techniques. Evidence for efficacy biological agents vegetal derivates has reported; their introduction into actual strategies requires solutions critical issues. Biotechnology-based approaches also explored, revealing opportunity develop safe methods management through genome editing RNA interference technologies. Nevertheless, besides number advantages related use, e.g., putative absence effects due high specificity, aspects remain be clarified enable Integrated Pest Management (IPM) protocols against disease.

Language: Английский

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Biotechnological and Biocontrol Approaches for Mitigating Postharvest Diseases Caused by Fungal Pathogens and Their Mycotoxins in Fruits: A Review

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(46), P. 17584 - 17596

Published: Nov. 8, 2023

Postharvest diseases caused by fungal pathogens are significant contributors to the postharvest losses of fruits. Moreover, some produce mycotoxins, which further compromise safety and quality In this review, potential biotechnological biocontrol approaches for mitigating mycotoxins in fruits is explored. The review begins discussing impact on fruit losses. Next, it provides an overview major pathogens. Subsequently, delves into role controlling these diseases. also explored application agents, such as antagonistic yeasts, bacteria, fungi, can suppress pathogen growth. Furthermore, future trends challenges two discussed detail. Overall, provide insights promising strategies managing

Language: Английский

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Spray-Induced Silencing of Pathogenicity Gene MoDES1 via Exogenous Double-Stranded RNA Can Confer Partial Resistance Against Fungal Blast in Rice

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Nov. 26, 2021

Over the past years, RNA interference (RNAi) has been used as a promising combat strategy against wide range of pests and pathogens in ensuring global food security. It involves induction highly specific posttranscriptional regulation target essential genes from an organism, via application precursor long, non-coding double-stranded (dsRNA) molecules that share sequence-complementarity with mRNAs targets. Fungal blast disease caused by Magnaporthe oryzae is one most deadly diseases rice wheat incurring huge losses crop yield. To date, host-induced gene silencing (HIGS) virus-induced (VIGS) aspects RNAi have successfully exploited developing resistance M. rice. Spray-induced (SIGS) current, potential, non-transformative, environment-friendly pest pathogen management strategy, where naked or nanomaterial-bound dsRNA are sprayed on leaves to cause selective knockdown pathogenicity genes. Although it relies ability fungal uptake RNA, its efficiency varies largely across phytopathogens their genes, targeted for silencing. Here, we report transient supplementation system MoDES1, host-defense suppressor oryzae. We validate feasibility vivo SIGS post-uptake transfer signals distal plant parts rice-M. pathosystem through GFP-based reporter system. A protocol efficient direct foliar spray was optimized. As proof-of-concept, demonstrate phenotypic impacts vitro dsDES1 treatment growth, conidiation, ROS-scavenging ability, pathogenic potential Furthermore, our extrapolatory experiments wounded whole plants indicate resultant MoDES1 conferred significant disease. The evaluation primary secondary host defense responses provides evidence supporting notion sequence-specific leaf tissue can systemic sustained gene. For first time, establish transgene-free approach protection notorious rice-blast fungus.

Language: Английский

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Will epigenetics be a key player in crop breeding?

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 30, 2022

If food and feed production are to keep up with world demand in the face of climate change, continued progress understanding utilizing both genetic epigenetic sources crop variation is necessary. Progress plant breeding has traditionally been thought be due selection for spontaneous DNA sequence mutations that impart desirable phenotypes. These can expand phenotypic diversity, from which breeders select agronomically useful traits. However, it become clear diversity generated even when genome unaltered. Epigenetic gene regulation a mechanism by expression regulated without altering sequence. With development high throughput sequencers, possible analyze state whole genome, termed epigenome. techniques enable us identify (epimutations) epimutations lead increased diversity. create new phenotypes causative inherited over generations. There evidence selected agronomic traits being conditioned heritable epimutations, may have historically epiallele-conditioned results imply not only but states contribute since modes induction transmission epialleles their stability differ alleles, importance inheritance as classically defined also differs. For example, there difference between types important production. The former depend more on longer-term whereas latter simply take advantage shorter-term phenomena. advances our epigenetics, epigenetics bring perspectives improvement, such use or epigenome editing breeding. In this review, we will introduce role breeding, largely focusing methylation, conclude asking what extent knowledge led documented cases its successful use.

Language: Английский

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Biotrophic Fungal Pathogens: a Critical Overview

Applied Biochemistry and Biotechnology, Journal Year: 2022, Volume and Issue: 195(1), P. 1 - 16

Published: Aug. 11, 2022

Language: Английский

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RNA-Based Vaccination of Plants for Control of Viruses

Annual Review of Virology, Journal Year: 2022, Volume and Issue: 9(1), P. 521 - 548

Published: Sept. 29, 2022

Plant viruses cause nearly half of the emerging plant diseases worldwide, contributing to 10–15% crop yield losses. Control viral is mainly accomplished by extensive chemical applications targeting vectors (i.e., insects, nematodes, fungi) transmitting these viruses. However, chemicals have a significant negative effect on human health and environment. RNA interference an endogenous, cellular, sequence-specific degradation mechanism in eukaryotes induced double-stranded molecules that has been exploited as antiviral strategy through transgenesis. Because genetically modified plants are not accepted for cultivation several countries globally, there urgent demand alternative strategies. This boosted research exogenous application RNA-based biopesticides shown exhibit protective against infections. Such environment-friendly efficacious agents protection will contribute global food security, without adverse effects health.

Language: Английский

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Strategies for Controlling the Sporulation in Fusarium spp.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 9(1), P. 10 - 10

Published: Dec. 21, 2022

Fusarium species are the most destructive phytopathogenic and toxin-producing fungi, causing serious diseases in almost all economically important plants. Sporulation is an essential part of life cycle Fusarium. frequently produces three different types asexual spores, i.e., macroconidia, chlamydospores, microconidia. It also meiotic but fewer than 20% Fusaria have a known sexual cycle. Therefore, spores play role their propagation infection. This review places special emphasis on current developments artificial anti-sporulation techniques as well features Fusarium's sporulation regulation, such temperature, light, pH, host tissue, nutrients. description regulation aspects strategies will help to shed light ways effectively control by inhibiting production which eventually improves food

Language: Английский

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Evaluation of the anti-viral efficacy of three different dsRNA nanoparticles against potato virus Y using various delivery methods

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 255, P. 114775 - 114775

Published: March 16, 2023

Nanoparticles (NPs) derived from RNA interference (RNAi) are considered a potentially revolutionary technique in the field of plant protection future. However, application NPs RNAi is hindered by conflict between high cost production and large quantity materials required for application. This study aimed to evaluate antiviral efficacy commercially available nanomaterials, such as chitosan quaternary ammonium salt (CQAS), amine functionalized silica nano powder (ASNP), carbon quantum dots (CQD), that carried double-stranded (dsRNA) via various delivery methods, including infiltration, spraying, root soaking. ASNP-dsRNA recommended soaking, which most effective method compound The tested was CQAS-dsRNA delivered Using fluorescence, FITC-CQAS-dsCP-Cy3, CQD-dsCP-Cy3 demonstrated uptake transport pathways dsRNA plants when applied different modes. duration with modes then compared, providing references evaluating retention period types NPs. All three effectively silenced genes afforded at least 14 days against viral infection. Particularly, CQD-dsRNA could protect systemic leaves 21 following spraying.

Language: Английский

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RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 9082 - 9082

Published: May 22, 2023

Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops causing significant yield losses. These obligate biotrophic parasites that completely dependent on their hosts for growth reproduction. Biotrophy in these is determined by the presence of haustoria, specialized fungal cells responsible nutrient uptake molecular dialogue with host, a fact undoubtedly complicates study under laboratory conditions, especially terms genetic manipulation. RNA interference (RNAi) biological process suppressing expression target gene through double-stranded induces mRNA degradation. RNAi technology has revolutionized enabling analysis function fungal. More importantly, opened new perspectives management powdery diseases, first stable constructs transgenic plants and, more recently, non-transgenic approach called spray-induced silencing (SIGS). In this review, impact research will be addressed.

Language: Английский

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