Plant Science Today,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 29, 2023
Drought
stress
is
one
of
the
most
considerable
threats
to
global
agricultural
food
security,
causing
yield
losses
worldwide.
Therefore,
search
for
effective
genetic
and
molecular
methods
developing
cultivars
that
are
tolerant
or
resistant
harsh
environments
has
been
more
intensive
over
last
decades.
Apart
from
time-consuming
conventional
breeding
techniques,
biotechnologists
now
investigating
modern
genome
editing
tools
engineering
tolerance
resistance
various
biotic
abiotic
stresses
in
crops.
Various
techniques
such
as
zinc
finger
nucleases
(ZFNs)
transcription
activator-like
effector
(TALENs)
were
developed
based
on
discovery
DNA
structure.
However,
these
have
limitations,
with
ZFNs
being
prone
errors
due
their
limited
base
pair
recognition,
TALENs
requiring
a
complex
protein
process
struggling
cleave
methylated
DNA.
In
recent
years,
clustered
regularly
interspaced
short
palindromic
repeats/CRISPR-associated
9
(CRISPR/Cas9)
its
alternatives
gained
popularity
plant
biotechnology.
Out
mentioned
earlier,
CRISPR/Cas9
becoming
popular
because
it's
faster
easier
use.
Given
drought
significant
threat
agriculture
drying
arable
lands,
this
review
focuses
how
we
can
use
CRISPR
enhance
crop
explores
future
potential.
BMC Biology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Jan. 17, 2024
Abstract
Background
Precise
gene
targeting
(GT)
is
a
powerful
tool
for
heritable
precision
genome
engineering,
enabling
knock-in
or
replacement
of
the
endogenous
sequence
via
homologous
recombination.
We
recently
established
CRISPR/Cas9-mediated
approach
GT
in
Arabidopsis
thaliana
(Arabidopsis)
and
rice
reported
that
double-strand
breaks
(DSBs)
frequency
Cas9
influences
efficiency.
However,
relationship
between
DSBs
at
same
locus
was
not
examined.
Furthermore,
it
has
never
been
investigated
whether
an
increase
number
copies
sgRNAs
use
multiple
would
improve
efficiency
GT.
Results
Here,
we
achieved
precise
loci
Embryo
Defective
2410
(
EMB2410
)
Repressor
Silencing
1
ROS1
using
sequential
transformation
strategy
combination
sgRNAs.
show
increasing
copy
elevates
both
On
other
hand,
application
does
always
enhance
Our
results
also
suggested
some
inefficient
play
role
as
helper
to
facilitate
activity.
Conclusions
The
this
study
clearly
DSB
efficiency,
rather
than
mutation
pattern,
one
most
important
key
factors
determining
This
provides
new
insights
into
sgRNAs,
DSBs,
GTs
molecular
mechanisms
plants.
Plant Biotechnology Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 27, 2025
Summary
Genome‐edited
plants,
endowed
with
climate‐smart
traits,
have
been
promoted
as
tools
for
strengthening
resilience
against
climate
change.
Successful
plant
gene
editing
(GE)
requires
precise
regulation
of
the
GE
machinery,
a
process
controlled
by
promoters,
which
drives
its
transcription
through
interactions
factors
(TFs)
and
RNA
polymerase.
While
constitutive
promoters
are
extensively
used
in
constructs,
their
limitations
highlight
need
alternative
approaches.
This
review
emphasizes
promise
tissue/organ
specific
well
inducible
enable
targeted
spatiotemporal
manner
no
effects
on
other
tissues.
Advances
synthetic
biology
paved
way
creation
offering
refined
control
over
expression
augmenting
potential
GE.
The
integration
these
novel
systems
presents
significant
opportunities
conditional
genome
editing.
Moreover,
advent
bioinformatic
artificial
intelligence
is
revolutionizing
characterization
regulatory
elements,
enhancing
our
understanding
roles
plants.
Thus,
this
provides
insights
into
strategic
use
promoter
to
enhance
precision,
efficiency
specificity
GE,
setting
stage
innovative
crop
improvement
strategies.
