Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells DOI Open Access
Mingyi Guo, Yan Deng,

Junqiu Huang

et al.

Polymers, Journal Year: 2023, Volume and Issue: 15(15), P. 3183 - 3183

Published: July 27, 2023

To guide therapeutic strategies and to monitor the state changes in disease, a low-cost, portable, easily fabricated microfluidic-chip-integrated three-dimensional (3D) microchamber was designed for capturing analyzing breast cancer cells. Optimally, colorimetric sensor array integrated into microfluidic chip discriminate metabolites of The ultraviolet polymerization characteristic poly(ethylene glycol) diacrylate (PEGDA) hydrogel utilized rapidly fabricate three-layer with structure under noninvasive 365 nm laser irradiation. 2-Hydroxyethyl methacrylate (HEMA) added prepolymer order increase adhesive capacity microchip’s surface 1-Vinyl-2-pyrrolidone (NVP) improve toughness reduce swelling composite. A non-toxic 3D microarray (60 mm × 20 3 mm) low immunogenicity high hydrophilicity created simulate real physiological microenvironment tissue. crisscross channels were ensure homogeneous seeding density. This material displayed excellent biocompatibility tunable physical properties compared traditional materials can be directly processed obtain most desirable microstructure. feasibility using PEGDA real-time online detection cells’ metabolism confirmed specifically 16 kinds porphyrin, porphyrin derivatives, indicator dyes. results principal component analysis (PCA), hierarchical cluster (HCA), linear discriminant (LDA) suggest that metabolic liquids different cells distinguished developed chip. has potential practicable applicability distinguishing normal cancerous

Language: Английский

Swellable colorimetric microneedles for glucose detection based on glucose oxidase-like gold nanoparticles DOI
Xiaodan Li,

Jiatong Lv,

Jiuhong Zhao

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1288, P. 342152 - 342152

Published: Dec. 16, 2023

Language: Английский

Citations

26
Remote‐Controlled Sensing and Drug Delivery via 3D‐Printed Hollow Microneedles DOI Creative Commons
Mahmood Razzaghi,

Joel Alexander Ninan,

Mostafa Azimzadeh

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Remote health monitoring and treatment serve as critical drivers for advancing equity, bridging geographical socioeconomic disparities, ensuring equitable access to quality healthcare those in underserved or remote regions. By democratizing healthcare, this approach offers timely interventions, continuous monitoring, personalized care independent of one's location status, thereby striving an distribution resources outcomes. Meanwhile, microneedle arrays (MNAs), revolutionize painless minimally invasive interstitial fluid drug delivery diagnostics. This paper introduces integrated theranostic MNA system employing array colorimetric sensors quantitatively measure -pH, glucose, lactate, alongside a remotely-triggered enabling on-demand delivery. Integration ultrasonic atomizer streamlines the delivery, facilitating rapid, pumpless, point-of-care enhancing portability while reducing complexities. An accompanying smartphone application interfaces sensing components. Demonstrated capabilities include detecting pH (3 8), glucose (up 16 mm), lactate 1.6 showcasing assessing performance via scratch assay. innovative confronts challenges, particularly managing chronic diseases requiring long-term treatment, also offering avenues non-invasive through microneedle-based sensors.

Language: Английский

Citations

15
Microenvironment-optimized GelMA microneedles for interstitial fluid extraction and real-time glucose detection DOI
Shixian Lin,

Yuehan Ouyang,

Wensheng Lin

et al.

Surfaces and Interfaces, Journal Year: 2024, Volume and Issue: 45, P. 103847 - 103847

Published: Jan. 4, 2024

Language: Английский

Citations

10
A swellable hydrogel microneedle based on cerium-metal organic frame composite nanozyme for detection of biomarkers DOI

Jiuhong Zhao,

Jiatong Lv,

Guixia Ling

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127745 - 127745

Published: Oct. 29, 2023

Language: Английский

Citations

20
3D bioprinted microneedles: merging drug delivery and scaffold science for tissue-specific applications DOI Creative Commons
Mershen Govender, Sunaina Indermun, Yahya E. Choonara

et al.

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(11), P. 1559 - 1572

Published: May 9, 2024

Three-Dimensional (3D) microneedles have recently gained significant attention due to their versatility, biocompatibility, enhanced permeation, and predictable behavior. The incorporation of biological agents into these 3D constructs has advanced the traditional microneedle an effective platform for wide-ranging applications.

Language: Английский

Citations

6
Microneedle-Integrated Sensors for Extraction of Skin Interstitial Fluid and Metabolic Analysis DOI Open Access
Jie Yang, Ruiyu Luo, Lei Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 9882 - 9882

Published: June 8, 2023

Skin interstitial fluid (ISF) has emerged as a fungible biofluid sample for blood serum and plasma disease diagnosis therapy. The sampling of skin ISF is highly desirable considering its easy accessibility, no damage to vessels, reduced risk infection. Particularly, can be sampled using microneedle (MN)-based platforms in the tissues, which exhibit multiple advantages including minimal invasion less pain, ease carrying, capacity continuous monitoring, etc. In this review, we focus on current development microneedle-integrated transdermal sensors collecting detecting specific biomarkers. Firstly, discussed classified microneedles according their structural design, solid MNs, hollow porous coated MNs. Subsequently, elaborate construction MN-integrated metabolic analysis with highlights electrochemical, fluorescent, chemical chromogenic, immunodiagnostic, molecular diagnostic sensors. Finally, discuss challenges future direction developing MN-based extraction sensing applications.

Language: Английский

Citations

16
The Effect of 3D Printing Tilt Angle on the Penetration of 3D-Printed Microneedle Arrays DOI Creative Commons
Mahmood Razzaghi, Mohsen Akbari

Micromachines, Journal Year: 2023, Volume and Issue: 14(6), P. 1157 - 1157

Published: May 30, 2023

Microneedle arrays (MNAs) are emerging devices that mainly used for drug delivery and diagnostic applications through the skin. Different methods have been to fabricate MNAs. Recently developed fabrication based on 3D printing many advantages compared conventional methods, such as faster in one step ability complex structures with precise control over their geometry, form, size, mechanical biological properties. Despite several offers of microneedles, poor penetration capability into skin should be improved. MNAs need a sharp needle tip penetrate barrier layer, stratum corneum (SC). This article presents method improve 3D-printed microneedle by investigating effect angle force The needed puncture fabricated using commercial digital light processing (DLP) printer, different tilt angles (0-60°), was measured this study. results showed minimum achieved 45° angle. Using angle, reduced 38% printed tilting 0°. We also identified 120° resulted smallest outcomes research show presented can significantly

Language: Английский

Citations

15
Epidermal Wearable Biosensors for the Continuous Monitoring of Biomarkers of Chronic Disease in Interstitial Fluid DOI Creative Commons

Xichen Yuan,

Oumaima Ouaskioud,

Xu Yin

et al.

Micromachines, Journal Year: 2023, Volume and Issue: 14(7), P. 1452 - 1452

Published: July 20, 2023

Healthcare technology has allowed individuals to monitor and track various physiological biological parameters. With the growing trend of use internet things big data, wearable biosensors have shown great potential in gaining access human body, providing additional functionality analyze biochemical information, which led a better personalized more efficient healthcare. In this review, we summarize biomarkers interstitial fluid, introduce explain extraction methods for discuss application epidermal continuous monitoring markers clinical biology. addition, current needs, development prospects challenges are briefly discussed.

Language: Английский

Citations

13
Integration of metformin-loaded MIL-100(Fe) into hydrogel microneedles for prolonged regulation of blood glucose levels DOI

Mingjia Feng,

Yan Li,

Yanfang Sun

et al.

Biomedical Physics & Engineering Express, Journal Year: 2024, Volume and Issue: 10(4), P. 045004 - 045004

Published: April 26, 2024

The transdermal drug delivery based on microneedles (MNs) provides a suitable and painless self-administration for diabetic patients. In this work, the hydrogel-forming MNs were firstly fabricated using poly(vinyl alcohol) (PVA) chitosan (CS) as matrix. A hypoglycemic drug, metformin (Met), had been loaded into MIL-100(Fe). Then, both of free Met Met-loaded MIL-100(Fe) integrated regulation blood glucose levels (BGLs) rats. After penetrated skin, could be released from MNs. Due to absorption interstitial fluid subsequent release MIL-100(Fe), leading sustainable long-term behaviors. notable effect low risk hypoglycemia obtained rat modelsin vivo. as-fabricated expected become new type platform high-dose drugs form effect.

Language: Английский

Citations

5
Porous Microneedles Through Direct Ink Drawing with Nanocomposite Inks for Transdermal Collection of Interstitial Fluid DOI

Yushuang Pang,

Yanyan Li, Kerong Chen

et al.

Small, Journal Year: 2024, Volume and Issue: 20(23)

Published: Jan. 23, 2024

Interstitial fluid (ISF) is an attractive alternative to regular blood sampling for health checks and disease diagnosis. Porous microneedles (MNs) are well suited collecting ISF in a minimally invasive manner. However, traditional methods of molding MNs from microfabricated templates involve prohibitive fabrication costs fixed designs. To overcome these limitations, this study presents facile economical additive manufacturing approach create porous MNs. Compared layerwise build sequences, direct ink drawing with nanocomposite inks can define sharp tailored shapes achieve vastly improved efficiency. The key strategy the yield-stress that easily formulated by dispersing silica nanoparticles into cellulose acetate polymer solution. As-printed solidified interconnected microstructure inside coagulation bath deionized water. resulting exhibit high mechanical strength porosity. This also allows be integrated on various substrates. In particular, filter paper substrates highly flexible rapidly collect non-flat skin sites. extracted used quantitative analysis biomarkers, including glucose, = calcium ions, ions. Overall, developments allow transdermal diagnosis therapy.

Language: Английский

Citations

4