Production and Characterization of Electrospun Chitosan, Nanochitosan and Hyaluronic Acid Membranes for Skin Wound Healing DOI
E Ponte, Alexia de Almeida Ignatowicz, Gabriel Raio Volpato

et al.

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2024, Volume and Issue: 112(10)

Published: Sept. 26, 2024

ABSTRACT The development of new wound dressings made from biomaterials, which offer a better cost–benefit ratio and accelerate the healing process, is increasing nowadays. Various biopolymers can be electrospun to form functional membranes for healing. Therefore, in this study, chitosan nanochitosan with or without hyaluronic acid were prepared using electrospinning technique, characterized evaluated skin wounds rats. Chitosan solutions, acid, at concentrations 1%–4% PEO (polyethylene oxide) subjected process obtain by scanning electron microscopy (SEM), mechanical tests, antimicrobial activity. effect was monitoring area lesions, contraction wounds, histologic analysis, induction pro‐inflammatory cytokine (IL‐1 α TNF‐α) production achieved greater fiber diameter uniformity, resistance, elasticity, thermal stability, addition good adhesion bed permeation capacity. Despite not presenting activity vitro, they contributed interleukins animals tested, provided physical protection, reduced more markedly until seventh day evaluation, an acceleration especially when functionalized acid. These results indicate that may promising accelerating chronic humans.

Language: Английский

Sodium alginate/chitosan composite scaffold reinforced with biodegradable polyesters/gelatin nanofibers for cartilage tissue engineering DOI
Chenxi Li, Wenbin Shang, Yuzhe Huang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138054 - 138054

Published: Nov. 1, 2024

Language: Английский

Citations

5
An overview of nasal cartilage bioprinting: from bench to bedside DOI
Hosein Rostamani, Omid Fakhraei,

Niloufar Zamirinadaf

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: 35(8), P. 1273 - 1320

Published: March 5, 2024

Nasal cartilage diseases and injuries are known as significant challenges in reconstructive medicine, affecting a substantial number of individuals worldwide. In recent years, the advent three-dimensional (3D) bioprinting has emerged promising approach for nasal reconstruction, offering potential breakthroughs field regenerative medicine. This paper provides an overview methods associated with 3D technologies procedure reconstructing tissue. The process entails generating digital model using biomedical imaging techniques computer-aided design to integrate both internal external scaffold features. Then, bioinks which consist biomaterials, cell types, bioactive chemicals, applied facilitate precise layer-by-layer tissue-engineered scaffolds. After undergoing vitro vivo experiments, this results development physiologically functional integrity advantages encompass ability customize design, enabling incorporation pore shape, size, porosity, well utilization patient-specific cells enhance compatibility. However, various should be considered, including optimization ensuring adequate viability differentiation, achieving seamless integration host tissue, navigating regulatory attention. Although numerous studies have demonstrated rebuilding such soft tissues, covers aspects bioprinted tissues provide insights future repair appropriate clinical use.

Language: Английский

Citations

4
Bioinspired PCL-Based Composite Scaffolds Produced via Hot Melt Extrusion and Fused Filament Fabrication: An Integrated Workflow for Enhanced Bone Regeneration DOI Creative Commons

Carmela Tommasino,

Carla Sardo,

Angiola Guidone

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106679 - 106679

Published: Feb. 1, 2025

Language: Английский

Citations

0
Current Topics in Dental Follicle Cell Research DOI Creative Commons
Christian Morsczeck

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(2)

Published: Feb. 12, 2025

Dental follicle cells (DFCs) are dental stem that can only be obtained from tooth germs or after extraction of unerupted wisdom teeth. For many years, DFCs have been studied in basic research and preclinical studies regenerative dentistry, as they involved both the development periodontium eruption. Since first isolation, number with has increased. This article summarizes most important articles last five years to provide an overview current topics. The focus was on research. Basic includes eruption, well into molecular mechanisms during osteogenic differentiation. In addition, focused therapies immunotherapies also discussed. These new show improved our understanding periodontal regeneration. DFC is for dentistry future; however, indicate significant progress still needed before integrated routine clinical practice.

Language: Английский

Citations

0
Biomechanical and histomorphometric evaluation of biodegradable mini-implants for orthodontic anchorage in the mandible of beagle dogs DOI Creative Commons
Shuo Wang, Zuodong Zhao, Q Zhang

et al.

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 10, 2025

To evaluate the effectiveness of a mini-implant composed unsintered hydroxyapatite, poly (L-lactic acid) and poly(lactic-co-glycolic (u-HA/PLLA/PLGA) composites as an anchorage device under consistent orthodontic force (OF) loading in vivo. An mandible model beagle dogs was introduced. 144 mini-implants were implanted both sides mandibles. The experimental group (left side) loaded at magnitude 200 g to simulate OF. At 2, 4 6 months after implantation, tissue specimens taken from sites biomechanical, histological histomorphometrical analysis performed. Mini-implants with highest PLLA ratio showed 27% non-fracture rate 20.83% dogs, non-fractured could maintain tensile g, while other two groups all fractured. Histomorphological that there no significant relationship between Bone Volume over Total (BV/TV) implantation time among most groups. level Bone-Implant Contact (BIC) Medium Low decreased gradually 2 months. This study biodegradable work alternative titanium alloy by adjusting proportion its ingredients. Degradable for lie their potential revolutionize treatments offering minimizes need secondary surgeries removal, thereby enhancing patient comfort reducing overall treatment time.

Language: Английский

Citations

0
Intra-abdominal transplantation of PLGA/PCL/M13 phage electrospun scaffold induces self-assembly of lymphoid tissue-like structure DOI Open Access
Zohreh Safari, Majid Sadeghizadeh, Ahmad Zavaran Hosseini

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 173, P. 116382 - 116382

Published: March 9, 2024

Lymphoid organs are the main structural components of immune system. In current research, mixture poly lactic-co-glycolic acid (PLGA), polycaprolactone (PCL), and M13 phage or its RGD-modified form was used in construction a fibrillar scaffold using electrospinning method. The constructs were transplanted intra-abdominally examined for formation lymphoid-like tissues at different time intervals. confocal scanning electron microscopy demonstrate that phage-containing scaffolds provide suitable environment lymph node-isolated fibroblasts. Morphological analysis node-like after transplantation. Histological confirm both blood angiogenesis implanted construct migration inflammatory cells to scaffolds. addition, flow cytometry immunohistochemistry showed homing compartmentalization dendritic (DCs), B T lymphocytes within PLGA/PCL/M13 phage-RGD based similar what is seen mouse lymphoid tissues. It seems application could improve generation functional electrospun be tissue regeneration.

Language: Английский

Citations

2
Development of Muscle Tendon Junction in vitro Using Aligned Electrospun PCL Fibres DOI Creative Commons
Nodoka Iwasaki, Marta Roldo, Aikaterina Karali

et al.

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(3), P. 409 - 420

Published: Jan. 24, 2024

The muscle tendon junction (MTJ) transmits the force generated by to and ultimately bone. Tears strains commonly occur at MTJ where regeneration is limited due poor vascularisation complexity of tissue. Currently treatments for a complete tear are often unsuccessful. creation tissue engineered would therefore be beneficial in development novel treatment. In this study, aligned electrospun polycaprolactone fibres were fabricated human myoblasts tenocytes cultured on scaffold. effect 10% cyclic strain co-culture formation was investigated. application significantly increased cell elongation, marker gene expression. Co-culture with induced higher expression compared separately. Paxillin collagen 22, naturally found MTJ, also produced when cells combined grown environment. For first time these results showed that combination promotes production proteins MTJ.

Language: Английский

Citations

1
Tissue engineering and future directions in regenerative medicine for knee cartilage repair: a comprehensive review DOI Creative Commons
Dragan Primorac, Vilim Molnar, Dimitrios Tsoukas

et al.

Croatian Medical Journal, Journal Year: 2024, Volume and Issue: 65(3), P. 268 - 288

Published: June 1, 2024

This review evaluates the current landscape and future directions of regenerative medicine for knee cartilage repair, with a particular focus on tissue engineering strategies. In this context, scaffold-based approaches have emerged as promising solutions regeneration. Synthetic scaffolds, while offering superior mechanical properties, often lack biological cues necessary effective integration. Natural though biocompatible biodegradable, frequently suffer from inadequate strength. Hybrid combining elements both synthetic natural materials, present balanced approach, enhancing support functionality. Advances in decellularized extracellular matrix scaffolds shown potential promoting cell infiltration integration native tissues. Additionally, bioprinting technologies enabled creation complex, bioactive that closely mimic zonal organization cartilage, providing an optimal environment growth differentiation. The also explores gene therapy editing techniques, including CRISPR-Cas9, to enhance repair by targeting specific genetic pathways involved these advanced therapies holds promise developing personalized durable treatments injuries osteoarthritis. conclusion, underscores importance continued multidisciplinary collaboration advance innovative bench bedside improve outcomes patients damage.

Language: Английский

Citations

1
Bioengineered cartilaginous grafts for repairing segmental mandibular defects DOI Creative Commons
D S Abdullah Al Maruf, Hai Xin, Kai Cheng

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Reconstructing critical-sized craniofacial bone defects is a global healthcare challenge. Current methods, like autologous transplantation, face limitations. Bone tissue engineering offers an alternative to bone, with traditional approaches focusing on stimulating osteogenesis via the intramembranous ossification (IMO) pathway. However, IMO falls short in addressing larger defects, particularly clinical scenarios where there insufficient vascularisation. This review explores redirecting regeneration through endochondral (ECO), process observed long healing stimulated by hypoxic conditions. Despite its promise, gaps exist applying ECO experiments, requiring elucidation of key aspects such as cell sources, biomaterials and priming protocols. discusses various scaffold cellular sources for chondrogenesis hypertrophic chondrocyte priming, mirroring The highlights challenges current proposes approaches. Emphasis segmental mandibular defect repair, offering insights future research application. concise aims advance critical strategies.

Language: Английский

Citations

1
Production and Characterization of Electrospun Chitosan, Nanochitosan and Hyaluronic Acid Membranes for Skin Wound Healing DOI
E Ponte, Alexia de Almeida Ignatowicz, Gabriel Raio Volpato

et al.

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2024, Volume and Issue: 112(10)

Published: Sept. 26, 2024

ABSTRACT The development of new wound dressings made from biomaterials, which offer a better cost–benefit ratio and accelerate the healing process, is increasing nowadays. Various biopolymers can be electrospun to form functional membranes for healing. Therefore, in this study, chitosan nanochitosan with or without hyaluronic acid were prepared using electrospinning technique, characterized evaluated skin wounds rats. Chitosan solutions, acid, at concentrations 1%–4% PEO (polyethylene oxide) subjected process obtain by scanning electron microscopy (SEM), mechanical tests, antimicrobial activity. effect was monitoring area lesions, contraction wounds, histologic analysis, induction pro‐inflammatory cytokine (IL‐1 α TNF‐α) production achieved greater fiber diameter uniformity, resistance, elasticity, thermal stability, addition good adhesion bed permeation capacity. Despite not presenting activity vitro, they contributed interleukins animals tested, provided physical protection, reduced more markedly until seventh day evaluation, an acceleration especially when functionalized acid. These results indicate that may promising accelerating chronic humans.

Language: Английский

Citations

0