ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(39), P. 40592 - 40607
Published: Sept. 20, 2024
This
study
introduces
a
novel,
environmentally
friendly
albumin
isolation
method
using
graphene
oxide
(GO).
GO
selectively
extracts
from
serum
samples,
leveraging
the
unique
interactions
between
GO's
oxygen-containing
functional
groups
and
proteins.
achieves
high
purification
efficiency
without
need
for
hazardous
chemicals.
Comprehensive
characterization
of
reduced
(rGO)
through
techniques
such
as
X-ray
diffraction
(XRD)
analysis,
Raman
spectroscopy,
scanning
electron
microscopy
(SEM),
Fourier
transform
infrared
spectroscopy
(FTIR)
confirmed
structural
group
transformations
crucial
protein
binding.
Sodium
dodecyl
sulfate-polyacrylamide
gel
electrophoresis
(SDS-PAGE)
mass
spectrometry
analyses
demonstrated
over
95%
purity
isolated
albumin,
with
minimal
contamination
other
The
developed
method,
optimized
pH
incubation
conditions,
showcases
green,
cost-effective,
simple
alternative
purification,
promising
broad
applicability
in
biomedical
research
clinical
applications.
Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
16(8), P. 1043 - 1043
Published: Aug. 6, 2024
Chitosan
nanoparticles
(CSNPs)
are
promising
vehicles
for
targeted
and
controlled
drug
release.
Recognized
their
biodegradability,
biocompatibility,
low
toxicity,
ease
of
production,
CSNPs
represent
an
effective
approach
to
delivery.
Encapsulating
drugs
within
(NPs)
provides
numerous
benefits
compared
free
drugs,
such
as
increased
bioavailability,
minimized
toxic
side
effects,
improved
delivery,
the
incorporation
additional
features
like
release,
imaging
agents,
combination
therapies
with
multiple
drugs.
Keys
parameters
in
nanomedicines
loading
content
efficiency.
Most
current
NP
systems
struggle
loading,
presenting
a
significant
challenge
field.
This
review
summarizes
recent
research
on
developing
high
capacity,
focusing
various
synthesis
strategies.
It
examines
CSNP
using
different
materials
providing
details
methods,
loadings,
encapsulation
efficiencies,
release
profiles,
stability,
applications
Additionally,
discusses
factors
affecting
valuable
guidelines
future
CSNPs’
development.
Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
17(2), P. 202 - 202
Published: Feb. 6, 2025
Background/Objectives:
The
principal
aim
of
this
work
was
to
prepare
a
liposomal
drug
delivery
system
based
on
the
commercial
doxorubicin
(DOX)
and
budding
agent
with
promising
anticancer
activity,
9-(N-piperazinyl)-5-methyl-12(H)-quino
[3,4-b][1,4]benzothiazinium
chloride
(9-PBThACl).
Methods:
A
spectrophotometric
methodology
used
meticulously
investigate
entrapment
release
characteristics
new
complexes
(L)
dipalmitoylphosphatidylcholine
(DPPC)
human
serum
albumin
(HSA)
its
defeated
analog
(dHSA).
Results:
impact
operational
parameters
(temperature
pH)
liposome/drug(s)/(d)HSA,
namely
[LDPPC/9-PBThACl/DOX
]:(d)HSA]
systems,
as
well
polarity
phospholipid
bilayer,
examined.
In
order
compare
experimental
findings,
mathematical
models
were
employed
specify
analytical
factors
controlling
process
release/potential
from
liposomes.
observed
variations
in
encapsulation
profiles
due
combination
conjugates
plasma
protein.
Conclusions:
It
proven
that
changes
environmental
pH
directly
affect
percentage
liposomes
medicine
efficiency.
Moreover,
grouping
tendency
combinations
investigated
using
component
analysis
(PCA)
hierarchical
clustering
(HCA).
Finally,
an
variance
(ANOVA)
confirmed
statistical
buffering
changing
temperature
conjugates.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 14, 2025
Targeted
delivery
of
therapeutic
agents
to
malignant
tissues
is
crucial
for
enhancing
clinical
outcomes
and
reducing
side
effects.
Magnetic
nanorobots
(MNRs)
present
a
promising
strategy
controlled
delivery,
leveraging
external
magnetic
fields
achieve
precise
in
vivo
targeting.
This
work
develops
elongated
MNRs
comprising
linearly
arranged
nanoparticles
linked
by
metal-polyphenol
complexes
(MPCs)
magnetic-field-directed
active
tumor
targeting
synergistic
therapy.
The
are
created
assembling
30
nm
Fe3O4
nanoparticles,
tannic
acid,
ferrous
ions
(Fe2+)
under
uniform
field,
resulting
chain-like
structures
fixed
MPCs,
which
also
promotes
peroxidase-like
activity.
These
show
greater
response
than
individual
offering
flexibility
manipulation.
MPCs
coating
allows
tailored
surface
modifications
with
glucose
oxidase,
copper
(Cu2+),
human
serum
albumin
(HSA),
producing
colloidally
stable
built-in
multienzymatic
cascade
(MNRs@GOx/Cu/HSA)
that
consumes
glucose,
generates
•OH,
depletes
the
antioxidant
glutathione
(GSH).
Collectively,
surface-engineered
multifunctional
demonstrate
improved
driven
fields,
leading
efficient
localized
chemodynamic
structural
functional
properties
developed
render
them
suitable
targeted
cargo
minimally
invasive
surgery,
treatments
disease
sites.
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 21, 2025
Angiogenesis
is
a
crucial
and
challenging
requirement
for
the
regeneration
repair
of
damaged
tissues,
particularly
critical-sized
ones.
To
address
this
challenge,
in
study,
we
fabricated
cell-communicating
gelatin
methacryloyl
(GelMA)
hydrogel
using
core-shell
silica
nanoparticles
conjugated
with
roxadustat
(FG-4592)
VEGF-mimetic
aptamer
(Apt02).
This
promotes
tube
formation
prevascularization
synergistically
through
both
extracellular
intracellular
pathways
human
umbilical
vein
endothelial
cells
(HUVEC),
FG-4592
acting
via
pathway
Apt02
pathway.
Fluorophore
carbon
quantum
dot
was
synthesized
used
as
core
constructing
amine-functionalized
(CQD@MSN-NH2).
Using
serum
albumin
(HSA)
protein
linker,
on
surface
to
finalized
CQD@MSN@HSA@FG-4592
(CMHF)
theranostic
proangiogenic
nanoparticle.
Several
techniques
were
characterize
structural
cytotoxic
properties
CMHF
nanoparticles.
On
other
hand,
incorporated
into
GelMA
induce
angiogenesis
extracellularly.
Results
showed
that
nanoparticle
are
cyto-compatible
periodontal
ligament
fibroblasts
(PDLF)
HUVEC.
The
HUVEC
assay
indicated
1.0
μM
Apt02,
20
FG-4592,
35
μg/mL
individually
induced
when
10
ng/mL
VEGF
positive
control.
Western
blot
quantitative
polymerase
chain
reaction
assays
four
genes
revealed
have
an
mechanism
action
while
increases
cellular
concentration
hypoxia-inducible
factor-1α
(Hif-1α)
transcription
factor
intercellularly
recruits
form
tube-like
vessels
vitro
ex
ovo.
In
summary,
our
study
introduces
injectable
containing
blend
5%
GelMA,
nanoparticles,
which
effectively
enhances
by
activating
(through
VEGFR)
(by
Hif-1α
overexpression)
more
effective
targeting
only
one
