Advancing Albumin Isolation from Human Serum with Graphene Oxide and Derivatives: A Novel Approach for Clinical Applications DOI Creative Commons

Chayachon Apiwat,

Jack W. Houghton, Ren Ren

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(39), P. 40592 - 40607

Published: Sept. 20, 2024

This study introduces a novel, environmentally friendly albumin isolation method using graphene oxide (GO). GO selectively extracts from serum samples, leveraging the unique interactions between GO's oxygen-containing functional groups and proteins. achieves high purification efficiency without need for hazardous chemicals. Comprehensive characterization of reduced (rGO) through techniques such as X-ray diffraction (XRD) analysis, Raman spectroscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) confirmed structural group transformations crucial protein binding. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) mass spectrometry analyses demonstrated over 95% purity isolated albumin, with minimal contamination other The developed method, optimized pH incubation conditions, showcases green, cost-effective, simple alternative purification, promising broad applicability in biomedical research clinical applications.

Language: Английский

The Uniqueness of Albumin as a Carrier in Nano Drug Delivery DOI Creative Commons
Yash Gupta,

Vishal Rai,

Anushka Singh

et al.

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(1), P. 7 - 11

Published: Feb. 1, 2024

The quest for precision medicine hinges on targeted drug delivery, minimizing off-target effects while maximizing therapeutic impact. Among nanocarriers, albumin – the most abundant protein in human blood emerges as a uniquely biocompatible stealth bomber. Its inherent advantages, including exceptional biodegradability, prolonged circulation, and natural affinity diseased tissues, perfectly align with goals of personalized medicine. Albumin readily solubilizes poorly soluble drugs, enhancing bioavailability broadening arsenal. versatile surface allows modifications, enabling dual diagnosis treatment (theranostics) tailored to individual needs. While challenges remain optimizing loading targeting specificity, albumin-based nanocarriers hold immense promise revolutionizing healthcare, delivering potent therapeutics pinpoint accuracy. burgeoning field nanodrug delivery seeks redefine landscapes by engineering nanoscale carriers that meticulously deliver drugs their designated targets, systemic exposure efficacy. This pursuit aligns medicine, where treatments demand exquisite control over delivery. Within this intricate choreography, albumin, resident plasma, maestro, orchestrating symphony advantages make it prime candidate nanocarrier construction.

Language: Английский

Citations

3
Molecular Ballet: Investigating the Complex Interaction between Self-Assembling Dendrimers and Human Serum Albumin via Computational and Experimental Methods DOI Creative Commons
Gabriele Cavalieri, Domenico Marson,

Nicoletta Giurgevich

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(4), P. 533 - 533

Published: April 12, 2024

Dendrimers, intricate macromolecules with highly branched nanostructures, offer unique attributes including precise control over size, shape, and functionality, making them promising candidates for a wide range of biomedical applications. The exploration their interaction biological environments, particularly human serum albumin (HSA), holds significant importance utilization. In this study, the between HSA recently developed self-assembling amphiphilic dendrimer (AD) was investigated using various experimental techniques. Fluorescence spectroscopy isothermal titration calorimetry revealed moderate interactions protein AD nanomicelles (NMs), primarily attributed to favorable enthalpic contributions arising from electrostatic hydrogen bonding. Structural analysis indicated minimal changes in upon complexation NMs, which further supported by computational simulations demonstrating stable at atomistic level. These findings provide valuable insights into binding mechanisms thermodynamic parameters governing HSA/AD NM interactions, thereby contributing understanding potential

Language: Английский

Citations

3
Strategies and Recent Advances on Improving Efficient Antitumor of Lenvatinib Based on Nanoparticle Delivery System DOI Creative Commons
Haiqing Wang,

Wentao Bo,

Xielin Feng

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 5581 - 5603

Published: June 1, 2024

Lenvatinib (LVN) is a potentially effective multiple-targeted receptor tyrosine kinase inhibitor approved for treating hepatocellular carcinoma, metastatic renal cell carcinoma and thyroid cancer. Nonetheless, poor pharmacokinetic properties including water solubility rapid metabolic, complex tumor microenvironment, drug resistance have impeded its satisfactory therapeutic efficacy. This article comprehensively reviews the uses of nanotechnology in LVN to improve antitumor effects. With characteristic high modifiability loading capacity nano-drug delivery system, an active targeting approach, controllable release, biomimetic strategies been devised deliver target tumors sequence, compensating lack passive targeting. The existing applications advances improving efficacy include longer-term efficiency, achieving higher combination therapy, tracking diagnosing application reducing toxicity. Therefore, using multiple combined with photothermal, photodynamic, immunoregulatory therapies overcomes multi-drug resistance, regulates unfavorable yields synergistic In brief, nano-LVN system has brought light war against cancer while at same time effect. More intelligent multifunctional nanoparticles should be investigated further converted into clinical future.

Language: Английский

Citations

3
Safe transportation and targeted destruction: Albumin encapsulated aggregation‐induced emission photosensitizer nanoaggregate for tumor photodynamic therapy through mitochondria damage‐triggered pyroptosis DOI Creative Commons
Juanmei Cao, Yong Qu, Shaojie Zhu

et al.

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Abstract Photodynamic therapy is a highly recommended alternative treatment for solid tumors, such as cutaneous or luminal in clinical practice. However, conventional photosensitizers (PSs) often induce undesirable phototoxic effects because of their normal tissue distribution and reduction antitumor resulting from aggregation‐caused quenching effects. The present study developed novel nano‐formulated aggregation‐induced emission (AIE)‐characteristic PS, nab‐TTVPHE, which composed human serum albumin carrier TTVPHE therapeutic agent, more effective cancer with lower Notably, the reactive oxygen species generated by were shielded nanoaggregate structure, photodynamic activity was after nanostructure dissociation. Nab‐TTVPHE actively internalized tumor cells via secreted protein, acidic rich cysteine released to form nanoaggregates. accumulated mitochondria, where it triggered mitochondrial damage under light irradiation its induced pyroptosis caspase‐3/gasdermin E (GSDME) signaling pathway kill cells. Therefore, this AIE‐characteristic PS provides an innovative strategy effect ability boost potential immunity GSDME‐mediated pyroptosis.

Language: Английский

Citations

3
Exploring the interaction of hesperetin-mediated green synthesised gold and silver nanoparticles with human serum albumin: A comparative analysis DOI
Aparna Raj,

L. Vidya,

T.N. Vipina Vinod

et al.

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: unknown, P. 126488 - 126488

Published: Nov. 1, 2024

Language: Английский

Citations

3
"Unlocking Therapeutic Precision: “Camptotheca acuminata, a Traditional Chinese Herb Tailored for Phytonano-Cancer Theranostics” DOI Creative Commons

Debojyoti Adak,

Priyanka Ray,

Saini Setua

et al.

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2024, Volume and Issue: 11, P. 100447 - 100447

Published: May 21, 2024

In recent years, there has been a growing interest in leveraging traditional medicinal plants for modern therapeutic approaches, particularly cancer treatment. The review explores the potential of Camptotheca acuminata, Chinese plant, treatment through innovative phytonano-based drug delivery systems. acuminata contains camptothecin, potent anticancer compound, but its use is hindered by poor solubility, bioavailability, and systemic toxicity. study highlights how phytonano-technology can enhance accuracy reduce side effects using nanoparticles to encapsulate selectively deliver drugs directly cells. This approach could significantly improve oncology therapy addressing limitations chemotherapy. Using PubMed Scopus, prospective research on activities several mushroom species during past 20 years were searched. query included "Camptotheca acuminata," "cancer," "nanoparticles," "traditional medicine," "theranostics." Only entire English-language articles used. Phytonano-Cancer Theranostics with camptothecin effectively targets cells, showing substantial uptake vitro prolonged circulation accumulation at tumor sites vivo. These results demonstrate precise effective therapy. integration offers promising targeted theranostic capabilities However, challenges remain, including optimizing nanoparticle design stability, scalability, biocompatibility, overcoming biological barriers efficient targeting. Addressing these requires interdisciplinary maximize strategies patient outcomes.

Language: Английский

Citations

2
Designing of Human Serum Albumin Nanoparticles for Drug Delivery: a Potential Use of Anticancer Treatment DOI Creative Commons
Ali W. Al-Ani, Rasha Al-Sahlanee

Karbala International Journal of Modern Science, Journal Year: 2024, Volume and Issue: 10(2)

Published: June 8, 2024

Human serum albumin (HSA) nanoparticles have been widely used as versatile drug delivery systems for improving the efficiency and pharmaceutical properties of drugs. The present study aimed to design HSA nanoparticle encapsulated with hydrophobic anticancer pyridine derivative (2-((2-([1,1'-biphenyl]-4-yl)imidazo[1,2-a]pyrimidin-3-yl)methylene)hydrazine-1-carbothioamide (BIPHC)). synthesis HSA-BIPHC was achieved using a desolvation process. Atomic force microscopy (AFM) analysis showed average size 80.21 nm. percentages entrapment efficacy, loading capacity production yield were 98.11%, 9.77% 91.29%, respectively. An In vitro release revealed that displayed fast dissolution at pH 7.4 compared 3.4. They also higher cytotoxic activity against MCF-7 human breast cancer cells. possible binding BIPHC into tyrosine threonine kinase (TTK) studied molecular modeling. findings this introduced promising candidate model delivering drug. These novel are characterized by their ability carry agent control improved targeting

Language: Английский

Citations

2
Stable Nitroxide as Diagnostic Tools for Monitoring of Oxidative Stress and Hypoalbuminemia in the Context of COVID-19 DOI Open Access
Ekaterina Georgieva, Julian Ananiev,

Y. Yovchev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8045 - 8045

Published: July 24, 2024

Oxidative stress is a major source of ROS-mediated damage to macromolecules, tissues, and the whole body. It an important marker in severe picture pathological conditions. The discovery free radicals biological systems gives "start" studying various processes related development progression many diseases. From this moment on, enrichment knowledge about participation free-radical pathogenesis cardiovascular, neurodegenerative, endocrine diseases, inflammatory conditions, infections, including COVID-19, increasing exponentially. Excessive responses abnormal reactive oxygen species (ROS) levels may disrupt mitochondrial dynamics, risk cell damage. In addition, low serum albumin changes normal physiological balance between reduced oxidized can be serious prerequisite for impaired antioxidant capacity body, worsening condition patients. This review presents interrelationship oxidative stress, inflammation, levels, which are hallmarks COVID-19.

Language: Английский

Citations

2
The hemostatic molecular mechanism of Sanguisorbae Radix’s pharmacological active components based on HSA: Spectroscopic investigations, molecular docking and dynamics simulation DOI Creative Commons

Fei Xu,

Yuqing Shen,

ZhiQiang Pan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e37020 - e37020

Published: Aug. 28, 2024

Language: Английский

Citations

2
Nano-based drug delivery systems in hepatocellular carcinoma DOI

Maryam Sadat Abtahi,

Alireza Fotouhi,

Niloufar Rezaei

et al.

Journal of drug targeting, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: June 7, 2024

The high recurrence rate of hepatocellular carcinoma (HCC) and poor prognosis after medical treatment reflects the necessity to improve current chemotherapy protocols, particularly drug delivery methods. Development targeted efficient systems (DDSs), in all active, passive stimuli-responsive forms for selective therapeutic drugs tumour site has been extended efficacy reduce severe side effects. Recent advances nanotechnology offer promising breakthroughs diagnosis, monitoring cancer cells. In this review, specific design DDSs based on different nano-particles their surface engineering is discussed. addition, innovative clinical studies which nano-based DDS was used HCC were highlighted.

Language: Английский

Citations

1