Electrochemical Analysis of the Interaction between DNA and Abiraterone D4A Metabolite DOI
Victoria V. Shumyantseva,

A. V. Berezhnova,

L.E. Agafonova

et al.

Journal of Analytical Chemistry, Journal Year: 2024, Volume and Issue: 79(7), P. 873 - 882

Published: July 1, 2024

Language: Английский

Pharmacogenomic Studies of Antiviral Drug Favipiravir DOI Creative Commons
Victoria V. Shumyantseva,

Tatiana V. Bulko,

Alexey A. Chistov

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(4), P. 503 - 503

Published: April 7, 2024

In this work, we conducted a study of the interaction between DNA and favipiravir (FAV). This chemotherapeutic compound is an antiviral drug for treatment COVID-19 other infections caused by RNA viruses. paper examines electroanalytical characteristics FAV. The determined concentrations correspond to therapeutically significant ones in range 50–500 µM (R2 = 0.943). We have shown that FAV can be electro-oxidized around potential +0.96 V ÷ +0.98 (vs. Ag/AgCl). A mechanism electrochemical oxidation was proposed. effect on recorded as changes intensity heterocyclic nucleobases (guanine, adenine thymine) using screen-printed graphite electrodes modified with single-walled carbon nanotubes titanium oxide nanoparticles. binding constants (Kb) FAV/dsDNA complexes guanine, thymine were calculated. values DNA-mediated decline coefficient calculated ratio signals presence electro-oxidation these bases without (S, %). Based analysis parameters, spectral data, intercalation proposed principal DNA. calf thymus also confirmed mechanism. However, additional mode interaction, such damage together electrostatic interactions, revealed prolonged exposure

Language: Английский

Citations

4
Electrochemical analysis of the interaction between DNA and abiraterone D4A metabolite DOI
Victoria V. Shumyantseva,

A. V. Berezhnova,

L.E. Agafonova

et al.

Журнал аналитической химии, Journal Year: 2025, Volume and Issue: 79(7), P. 733 - 745

Published: Jan. 6, 2025

Language: Английский

Citations

0
Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA DOI Creative Commons

Veronica V. Pronina,

L.V. Kostryukova,

Tatiana V. Bulko

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5317 - 5317

Published: July 10, 2023

The interactions of dsDNA with new targeted drug delivery derivatives doxorubicin (DOX), such as DOX embedded into phospholipid nanoparticles (NPhs) and the NGR peptide-modified NPhs were studied electrochemically by differential pulse voltammetry technique. Screen-printed electrodes (SPEs), modified stable fine dispersions carbon nanotubes (CNTs), used for quantitative electrochemical investigations direct oxidation guanine, adenine, thymine heterocyclic bases dsDNA, their changes in presence nanoderivatives. Analysing shifts peak potentials nucleobases drug, we have shown that peptide changed mode interaction DNA-drug complexes from intercalative to electrostatic. Binding constants (Kb) calculated accordance signals. Based on our experiments, proven surface modification a system dramatically mechanism genetic material. DNA-mediated toxicity was based concentration-dependent "response" influence. DOX, DOX-loaded (NPhs), addressed moderately toxic concentration range 0.5-290 µM.

Language: Английский

Citations

9
Electrochemical approach for the analysis of DNA degradation in native DNA and apoptotic cells DOI Creative Commons

Lyubov E. Agafonova,

Dmitry D. Zhdanov, Yulia A. Gladilina

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e25602 - e25602

Published: Feb. 1, 2024

The aim of this work was to develop an electrochemical approach for the analysis DNA degradation and fragmentation in apoptotic cells. damage is considered one major causes human diseases. We analyzed cleavage processes circular plasmid pTagGFP2-N calf thymus DNA, which were exposed restriction endonucleases (the BstMC I AluB nonspecific endonuclease I). Genomic from leukemia K562 cell line used as a marker early late (mature) stages apoptosis. Registration direct oxidation nucleobases molecules subjected or apoptosis proposed detection these biochemical events. Label-free differential pulse voltammetry (DPV) has been measure activities using carbon nanotube-modified electrodes. present DPV technique provides promising platform high-throughput screening hydrolases registering efficiency processes. comparative its native supercoiled state plasmids restricted 4 23 parts revealed significant differences their behavior. Electrochemical fully confirmed by means traditional methods registration process, such gel electrophoresis flow cytometry.

Language: Английский

Citations

3
Electroanalysis in Pharmacogenomic Studies: Mechanisms of Drug Interaction with DNA DOI
Victoria V. Shumyantseva,

Veronica V. Pronina,

Tatiana V. Bulko

et al.

Biochemistry (Moscow), Journal Year: 2024, Volume and Issue: 89(S1), P. S224 - S233

Published: Jan. 1, 2024

Language: Английский

Citations

2
Electrochemical Analysis of the Interaction between DNA and Abiraterone D4A Metabolite DOI
Victoria V. Shumyantseva,

A. V. Berezhnova,

L.E. Agafonova

et al.

Journal of Analytical Chemistry, Journal Year: 2024, Volume and Issue: 79(7), P. 873 - 882

Published: July 1, 2024

Language: Английский

Citations

0