Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone DOI
Zheng Li, Shengwei Zhang, Tao Yu

et al.

Analytical Chemistry, Journal Year: 2019, Volume and Issue: 91(16), P. 10448 - 10457

Published: June 13, 2019

Developing easy-to-use and miniaturized detectors is essential for in-field monitoring of environmentally hazardous substances, such as the cyanotoxins. We demonstrated a differential fluorescent sensor array made aptamers single-stranded DNA (ssDNA) dyes multiplexed detection discrimination four common cyanotoxins with an ordinary smartphone within 5 min reaction. The assay reagents were preloaded dried in microfluidic chip long shelf life over 60 days. Upon addition analyte solutions, competitive binding cyanotoxin to specific aptamer-dye conjugate occurred. A zone-specific concentration-dependent reduction green fluorescence was observed result aptamer conformation change. aptasensors are fully optimized by quantification their dissociation constants, tuning stoichiometric ratios reaction mixtures, implementation internal intensity correction step. allowed accurate identification measurement important cyanotoxins, including anatoxin-a (ATX), cylindrospermopsin (CYN), nodularin (NOD), microcystin-LR (MC-LR), parallel, limit (LOD) down few nanomolar (<3 nM), which close World Health Organization's guideline maximum concentration drinking water. smartphone-based platform also showed remarkable chemical specificity against potential interfering agents performance system tested validated real lake water samples that contaminated trace levels individual well binary, ternary, quaternary mixtures. Finally, app interface has been developed rapid on-site data processing display.

Language: Английский

Electrochemical biosensors for pathogen detection DOI Creative Commons
Ellen Cesewski, Blake N. Johnson

Biosensors and Bioelectronics, Journal Year: 2020, Volume and Issue: 159, P. 112214 - 112214

Published: April 12, 2020

Language: Английский

Citations

716

Immobilized Enzymes in Biosensor Applications DOI Open Access
Hoang Hiep Nguyen, Sun Hyeok Lee,

Ui Jin Lee

et al.

Materials, Journal Year: 2019, Volume and Issue: 12(1), P. 121 - 121

Published: Jan. 2, 2019

Enzyme-based biosensing devices have been extensively developed over the last few decades, and proven to be innovative techniques in qualitative quantitative analysis of a variety target substrates wide range applications. Distinct advantages that enzyme-based biosensors provide, such as high sensitivity specificity, portability, cost-effectiveness, possibilities for miniaturization point-of-care diagnostic testing make them more attractive research focused on clinical analysis, food safety control, or disease monitoring purposes. Therefore, this review article investigates operating principle enzymatic utilizing electrochemical, optical, thermistor, piezoelectric measurement their applications literature, well approaches improving use enzymes biosensors.

Language: Английский

Citations

517

Biosorption: An Interplay between Marine Algae and Potentially Toxic Elements—A Review DOI Creative Commons
Muhammad Bilal, Tahir Rasheed, Juan Eduardo Sosa‐Hernández

et al.

Marine Drugs, Journal Year: 2018, Volume and Issue: 16(2), P. 65 - 65

Published: Feb. 19, 2018

In recent decades, environmental pollution has emerged as a core issue, around the globe, rendering it of fundamental concern to eco-toxicologists, biologists, eco-chemists, pathologists, and researchers from other fields. The dissolution polluting agents is leading cause all key spheres including hydrosphere, lithosphere, biosphere, among others. widespread occurrence various pollutants toxic heavy metals emerging hazardous contaminants serious concern. With increasing scientific knowledge, socioeconomic awareness, human health problems, ecological apprehensions, people are more concerned about adverse outcomes. Against this background, several removal methods have been proposed implemented with aim addressing sustainable eco-friendly development. Among them, biosorption using naturally inspired sources, e.g., marine algae, considerable advantages. past few years, algae extensively studied due their natural origin, overall cost-effective ratio, effectiveness against broader pollutant range; thus, they considered potential alternative conventional used for decontamination. Herein, an effort made highlight importance biosorbents role in biosorption. Biosorption mechanisms factors affecting activities also discussed review. utilization biosorbent numerous potentially elements reviewed.

Language: Английский

Citations

411

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis DOI Creative Commons
Hui Chen, Olja Simoska, Koun Lim

et al.

Chemical Reviews, Journal Year: 2020, Volume and Issue: 120(23), P. 12903 - 12993

Published: Oct. 14, 2020

Bioelectrocatalysis is an interdisciplinary research field combining biocatalysis and electrocatalysis via the utilization of materials derived from biological systems as catalysts to catalyze redox reactions occurring at electrode. synergistically couples merits both electrocatalysis. The advantages include high activity, selectivity, wide substrate scope, mild reaction conditions. possible renewable electricity electron source energy conversion efficiency. These properties are integrated achieve selective biosensing, efficient conversion, production diverse products. This review seeks systematically comprehensively detail fundamentals, analyze existing problems, summarize development status applications, look toward future directions bioelectrocatalysis. First, structure, function, modification bioelectrocatalysts discussed. Second, essentials bioelectrocatalytic systems, including transfer mechanisms, electrode materials, medium, described. Third, application bioelectrocatalysis in fields biosensors, fuel cells, solar catalytic mechanism studies, bioelectrosyntheses high-value chemicals summarized. Finally, developments a perspective on suggested.

Language: Английский

Citations

325

Biosensors for wastewater monitoring: A review DOI
Fatemeh Ejeian,

Parisa Etedali,

Hajar-Alsadat Mansouri-Tehrani

et al.

Biosensors and Bioelectronics, Journal Year: 2018, Volume and Issue: 118, P. 66 - 79

Published: July 14, 2018

Language: Английский

Citations

254

Towards a point-of-care SERS sensor for biomedical and agri-food analysis applications: a review of recent advancements DOI
Jayakumar Perumal, Yusong Wang, Amalina Binte Ebrahim Attia

et al.

Nanoscale, Journal Year: 2020, Volume and Issue: 13(2), P. 553 - 580

Published: Dec. 9, 2020

The growing demand for reliable and robust methodology in bio-chemical sensing calls the continuous advancement of sensor technologies. Over last two decades, surface-enhanced Raman spectroscopy (SERS) has emerged as one most promising analytical techniques sensitive trace analysis or detection biomedical agri-food applications. SERS overcomes inherent sensitivity limitation associated with spectroscopy, which provides vibrational "fingerprint" spectra molecules that makes it unique versatile among other techniques. This paper comprehensively reviews recent advancements biomedical, food agricultural applications over 6 years, we envision that, near future, some these platforms have potential to be translated a point-of-care rapid real-life end-user merits limitations various designs are analysed discussed based on critical features such sensitivity, specificity, usability, repeatability reproducibility. We conclude by highlighting opportunities challenges field while stressing technological gaps addressed realizing commercially viable sensors practical

Language: Английский

Citations

180

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress DOI Creative Commons
Tony O Hara, Baljit Singh

ACS ES&T Water, Journal Year: 2021, Volume and Issue: 1(3), P. 462 - 478

Published: Feb. 15, 2021

The release of chemicals into water systems has resulted in pollution many parts the world, threatening human health and aquatic ecosystems. Sources chemical discharge include industry, agricultural, wastewater treatment plants, stormwater overflows. To combat pollution, European Union introduced several directives that set concentration limits for drinking water, surface groundwater. meet these limits, it is essential rapid, reliable, sensitive analytical detection be developed put use. This Review presents progress made development electrochemical biosensors environmental toxicants (pesticides heavy metals) over past seven years (2014–2020). For those unfamiliar with this field research, concept a biosensor followed by critical evaluation their performance detecting toxicants. Current challenges are discussed as well potential avenues future including demands enhanced performance, improved stability shelf life, greater integration microfluidic devices wireless database technologies remote sensing applications. We believe will beneficial enhance awareness appreciation role can play protecting our environment resources.

Language: Английский

Citations

169

Biosensors for rapid detection of bacterial pathogens in water, food and environment DOI Creative Commons
Raphael Chukwuka Nnachi, Ning Sui, Bowen Ke

et al.

Environment International, Journal Year: 2022, Volume and Issue: 166, P. 107357 - 107357

Published: June 18, 2022

Conventional techniques (e.g., culture-based method) for bacterial detection typically require a central laboratory and well-trained technicians, which may take several hours or days. However, recent developments within various disciplines of science engineering have led to major paradigm shift in how microorganisms can be detected. The analytical sensors are widely used medical applications the literature being extended rapid on-site monitoring pathogens food, water environment. Especially, low-resource settings such as low middle-income countries, due advantages cost, rapidness potential field-testing, their use is indispensable sustainable development regions. Within this context, paper discusses methods biosensors ensure food safety, quality environmental monitoring. In brief, most our discussion focused on including microfluidic chips. performances sensitivity, specificity usability these sensors, well brief comparison with conventional bacteria detection, form core part discussion. Furthermore, we provide holistic viewpoint future research should focus exploring synergy different sensing technologies by developing an integrated multiplexed, sensitive accurate that will enable

Language: Английский

Citations

155

Are plasmonic optical biosensors ready for use in point-of-need applications? DOI Creative Commons
Juanjuan Liu, Mahsa Jalali, Sara Mahshid

et al.

The Analyst, Journal Year: 2019, Volume and Issue: 145(2), P. 364 - 384

Published: Dec. 12, 2019

Plasmonics has drawn significant attention in the area of biosensors for decades due to unique optical properties plasmonic resonant nanostructures. While sensitivity and specificity molecular detection relies significantly on resonance conditions, been dedicated design, fabrication, optimization substrates. The adequate choice materials, structures, functionality goes hand with a fundamental understanding plasmonics enable development practical that can be deployed real life situations. Here we provide brief review detailing most recent developments applications. Besides metals, novel materials such as graphene are highlighted. Sensors based Surface Plasmon Resonance (SPR), Localized (LSPR), Enhanced Raman Spectroscopy (SERS) presented classified their structure. In addition, applications environment monitoring, health diagnosis, food safety presented. Potential problems related implementation discussed an outlook is

Language: Английский

Citations

153

Recent Trends in Biosensors for Environmental Quality Monitoring DOI Creative Commons
Simona Gavrilaș,

Claudiu Ștefan Ursachi,

Simona Perța-Crișan

et al.

Sensors, Journal Year: 2022, Volume and Issue: 22(4), P. 1513 - 1513

Published: Feb. 15, 2022

The monitoring of environmental pollution requires fast, reliable, cost-effective and small devices. This need explains the recent trends in development biosensing devices for pollutant detection. present review aims to summarize newest regarding use biosensors detect contaminants. Enzyme, whole cell, antibody, aptamer, DNA-based biomimetic sensors are discussed. We their applicability detection various pollutants mention constructive characteristics. Several principles used biosensor design: amperometry, conductometry, luminescence, etc. They differ terms rapidity, sensitivity, profitability, design. Each one is characterized by specific selectivity limits depending on sensitive element. Mimetic slowly gaining attention from researchers users due advantages compared with classical ones. Further studies necessary robust that can successfully be complex matrices without prior sample preparation.

Language: Английский

Citations

135