MugenNet: A Novel Combined Convolution Neural Network and Transformer Network with Application in Colonic Polyp Image Segmentation DOI Creative Commons
Chen Peng, Zhiqin Qian,

Kunyu Wang

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(23), P. 7473 - 7473

Published: Nov. 23, 2024

Accurate polyp image segmentation is of great significance, because it can help in the detection polyps. Convolutional neural network (CNN) a common automatic method, but its main disadvantage long training time. Transformer another method that be adapted to by employing self-attention mechanism, which essentially assigns different importance weights each piece information, thus achieving high computational efficiency during segmentation. However, potential drawback with risk information loss. The study reported this paper employed well-known hybridization principle propose combine CNN and retain strengths both. Specifically, applied early colonic polyps implement model called MugenNet for We conducted comprehensive experiment compare other models on five publicly available datasets. An ablation was as well. experimental results showed achieve mean Dice 0.714 ETIS dataset, optimal performance dataset compared models, an inference speed 56 FPS. overall outcome optimally two methods machine learning are complementary other.

Language: Английский

Nanotechnology-Based Diagnostics for Diseases Prevalent in Developing Countries: Current Advances in Point-of-Care Tests DOI Creative Commons
Lungile Nomcebo Thwala,

Sphumelele Colin Ndlovu,

Kelvin Mpofu

et al.

Nanomaterials, Journal Year: 2023, Volume and Issue: 13(7), P. 1247 - 1247

Published: March 31, 2023

The introduction of point-of-care testing (POCT) has revolutionized medical by allowing for simple tests to be conducted near the patient's care point, rather than being confined a laboratory. This been especially beneficial developing countries with limited infrastructure, where often involves sending specimens off-site and waiting hours or days results. However, development POCT devices challenging, simplicity, accuracy, cost-effectiveness key factors in making these feasible. Nanotechnology played crucial role achieving this goal, not only possible but also masking their complexity. In article, recent developments that benefit from nanotechnology are discussed. Microfluidics lab-on-a-chip technologies highlighted as major drivers testing, particularly infectious disease diagnosis. These enable various bioassays used at point care. article addresses challenges faced technological advances interesting future trends. benefits significant, is shifting towards prevention, early detection, managing chronic conditions. Infectious low-income can lead prompt treatment, preventing infections spreading.

Language: Английский

Citations

70
Saliva-based microfluidic point-of-care diagnostic DOI Creative Commons

Trey W. Pittman,

Daniel Balazs Decsi,

Chamindie Punyadeera

et al.

Theranostics, Journal Year: 2023, Volume and Issue: 13(3), P. 1091 - 1108

Published: Jan. 1, 2023

There has been a long-standing interest in point-of-care (POC) diagnostics as tool to improve patient care because it can provide rapid, actionable results near the patient.Some of successful examples POC testing include lateral flow assays, urine dipsticks, and glucometers.Unfortunately, analysis is somewhat limited by ability manufacture simple devices selectively measure disease specific biomarkers need for invasive biological sampling.Next generation POCs are being developed that make use microfluidic detect fluids non-invasive manner, addressing above-mentioned limitations.Microfluidic desirable they perform additional sample processing steps not available existing commercial diagnostics.As result, more sensitive selective analysis.While most methods blood or matrix, there growing push saliva diagnostic medium.Saliva represents an ideal biofluid detecting readily large quantities analyte levels reflect those blood.However, using relatively new emerging field.The overarching aim this review update on recent literature focused matrix devices.We will first cover characteristics medium then salivary biomarkers.

Language: Английский

Citations

50
Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market DOI Creative Commons
A. Akhlaghi, Harmanjit Kaur, Bal Ram Adhikari

et al.

ECS Sensors Plus, Journal Year: 2024, Volume and Issue: 3(1), P. 011601 - 011601

Published: March 1, 2024

There is a plethora of electrochemical biosensors developed for ultrasensitive detection clinically relevant biomarkers. However, many these systems lose their performance in heterogeneous clinical samples and are too complex to be operated by end users at the point-of-care (POC), prohibiting commercial success. Integration with sample processing technology addresses both challenges; however, it adds manufacturing complexity overall cost systems. Herein, we review different components biosensor avenues creating fully integrated In context integration, focus on discussing trade-offs between sensing performance, cost, scalable guide readers toward designing new commercialization potential.

Language: Английский

Citations

35
NFC Smartphone-Based Electrochemical Microfluidic Device Integrated with Nanobody Recognition for C-Reactive Protein DOI Creative Commons
Suchanat Boonkaew, Katarzyna Szot-Karpińska, Joanna Niedziółka‐Jönsson

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(6), P. 3066 - 3074

Published: June 15, 2024

Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics, and the integration of biorecognition elements with electronic components into these widens their functionalities facilitates development complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies to capture relevant biomarkers are often limited in terms sensitivity, selectivity, storage stability, considerably restricting use POCT real-world applications. Therefore, we used nanobodies they more suitable fabricating electrochemical near-field communication (NFC) technology. Moreover, flow-through microfluidic device was implemented this system detection C-reactive protein (CRP), an inflammation biomarker, model analyte. The resulting sensors not only have high sensitivity portability but also retain automated sequential flow properties through capillary transport without need external pump. We compared accuracy CRP analyses between commercial PalmSens4 NFC-based potentiostats. Furthermore, sensor reliability evaluated using three biological samples (artificial serum, plasma, whole blood any pretreatment). This platform will streamline by combining operational simplicity, low cost, fast analysis, portability.

Language: Английский

Citations

17
CRISPR-Cas-mediated diagnostics DOI

Luoxi Li,

Guanwang Shen, Min Wu

et al.

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 40(11), P. 1326 - 1345

Published: May 17, 2022

Language: Английский

Citations

48
Modern technologies and solutions to enhance surveillance and response systems for emerging zoonotic diseases DOI Creative Commons
Li Zhang, Wenqiang Guo,

Chenrui Lv

et al.

Science in One Health, Journal Year: 2023, Volume and Issue: 3, P. 100061 - 100061

Published: Dec. 12, 2023

Zoonotic diseases originating from animals pose a significant threat to global public health. Recent outbreaks, such as COVID-19, have caused widespread illness, death, and socioeconomic disruptions worldwide. To effectively combat these diseases, it is crucial strengthen surveillance capabilities establish rapid response systems. This review examines modern technologies solutions that the potential enhance zoonotic disease outbreak response. The discusses advanced tools including big data analytics, artificial intelligence, Internet of Things, geographic information systems, remote sensing, molecular diagnostics, point-of-care testing, telemedicine, digital contact tracing, early warning These enable real-time monitoring, prediction risks, anomaly detection, diagnosis, targeted interventions during outbreaks. When integrated thoughtfully through collaborative partnerships, they significantly improve speed effectiveness control. However, several challenges persist, particularly in resource-limited settings, infrastructure limitations, costs, integration, training requirements, ethical implementation. With strategic planning coordinated efforts, offer immense bolster response, serving critical arsenal against emerging threats

Language: Английский

Citations

36
Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection DOI Creative Commons
Lulu Liu, Wenrui Ma, Xiang Wang

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 350 - 350

Published: March 6, 2023

There are various pathogenic bacteria in the surrounding living environment, which not only pose a great threat to human health but also bring huge losses economic development. Conventional methods for detection usually time-consuming, complicated and labor-intensive, cannot meet growing demands on-site rapid analyses. Sensitive, effective necessary environmental monitoring, food safety infectious diagnosis. Recently, benefiting from its advantages of rapidity high sensitivity, surface-enhanced Raman spectroscopy (SERS) has attracted significant attention field identification as well drug susceptibility testing. Here, we comprehensively reviewed latest advances SERS technology analysis. Firstly, mechanism fabrication substrate were briefly introduced. Secondly, label-free applied species was summarized detail. Thirdly, tags high-sensitivity discussed. Moreover, emphasized application prospects microfluidic chips antimicrobial testing (AST). In end, gave an outlook on future development trends point-of-care diagnoses bacterial infections.

Language: Английский

Citations

35
Multiplex Detection of Infectious Diseases on Microfluidic Platforms DOI Creative Commons
Fumin Chen, Qinqin Hu, Huimin Li

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 410 - 410

Published: March 21, 2023

Infectious diseases contribute significantly to the global disease burden. Sensitive and accurate screening methods are some of most effective means identifying sources infection controlling infectivity. Conventional detecting strategies such as quantitative polymerase chain reaction (qPCR), DNA sequencing, mass spectrometry typically require bulky equipment well-trained personnel. Therefore, a large population using conventional during pandemic periods often requires additional manpower, resources, time, which cannot be guaranteed in resource-limited settings. Recently, emerging microfluidic technologies have shown potential replace performing point-of-care detection because they automated, miniaturized, integrated. By exploiting spatial separation sites, platforms can enable multiplex infectious reduce possibility misdiagnosis incomplete diagnosis with similar symptoms. This review presents recent advances used for diseases, including immunosensors nucleic acid sensors. As representative platforms, lateral flow immunoassay (LFIA) polymer-based chips, paper-based devices, droplet-based devices will discussed detail. In addition, current challenges, commercialization, prospects proposed promote application detection.

Language: Английский

Citations

25
Point-of-care devices engaging green graphene: an eco-conscious and sustainable paradigm DOI
Joydip Sengupta, Chaudhery Mustansar Hussain

Nanoscale, Journal Year: 2024, Volume and Issue: 16(14), P. 6900 - 6914

Published: Jan. 1, 2024

The healthcare landscape has experienced a profound and irreversible transformation, primarily driven by the emergence of green nanomaterial-assisted point-of-care (POC) devices.

Language: Английский

Citations

9
Integrating of analytical techniques with enzyme-mimicking nanomaterials for the fabrication of microfluidic systems for biomedical analysis DOI
Sherzod Abdullaev,

Raed H. Althomali,

Ahmad Raza Khan

et al.

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125896 - 125896

Published: March 12, 2024

Language: Английский

Citations

8