Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications DOI Creative Commons

Maitham Najim Aboud,

K. H. Al–Sowdani

Pakistan Journal of Analytical & Environmental Chemistry, Journal Year: 2024, Volume and Issue: 25(1), P. 13 - 21

Published: June 28, 2024

Microfluidics can process small amounts of fluids by using microscopic channels with microscale dimensions ranging from tens to hundreds micrometers. Many researchers have recently been working on smaller analytical equipment, such as micro-flow cytometers. These gadgets offer numerous advantages, including portability, low cost, and power consumption. It also be integrated other microfluidic devices for multitasking applications. In this study, the fluorescein dye was estimated a lab-builtfully automated fluorometric system. The developed system contained chip two lines, each four centimetres long volume 15 μL. setup, Arduino UNO (R3 version) microcontrollers were programmed homebrew software. first manage improvised peristaltic pumps, which successfully introduced water stream automatically, replacing manual injection. sample modified varying line's run time. second one utilized record output signal. method accurately identified concentrations up 0.01 μg/mL 0.999 regression coefficient six points. RSD% (Relative Standard Deviation) ten 0.08 measurements 0%, detection limit 1x10-4 μg/mL. suggested measure 300 samples per hour while decreasing reagents waste. This has potential used fluorescent assays in variety

Language: Английский

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine DOI Creative Commons
Ying Bao,

Dejing Zhang,

Huihui� Guo

et al.

Cancer Science, Journal Year: 2024, Volume and Issue: 115(4), P. 1060 - 1072

Published: Feb. 3, 2024

Abstract Liquid biopsy is emerging as a pivotal tool in precision oncology, offering noninvasive and comprehensive approach to cancer diagnostics management. By harnessing biofluids such blood, urine, saliva, cerebrospinal fluid, pleural effusions, this technique profiles key biomarkers including circulating tumor DNA, cells, microRNAs, extracellular vesicles. This review discusses the extended scope of liquid biopsy, highlighting its indispensable role enhancing patient outcomes through early detection, continuous monitoring, tailored therapy. While advantages are notable, we also address challenges, emphasizing necessity for precision, cost‐effectiveness, standardized methodologies broader application. The future trajectory set expand reach personalized medicine, fueled by technological advancements collaborative research.

Language: Английский

Citations

22
Synergistic effects of dielectrophoretic and magnetophoretic forces on continuous cell separation via pinched flow fractionation DOI

Mahdi Zare,

Mahdi Khatibi, Seyed Nezameddin Ashrafizadeh

et al.

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(2)

Published: Feb. 1, 2025

The challenges of constraints and imprecision in chemical analysis medical diagnosis are particularly evident the separation blood cells. Pinched flow fractionation, a passive microfluidic technique, has gained attention for its potential size-based cell separation. Enhancing efficiency is crucial, especially through integration with external forces or active methods. This study explores use dielectrophoresis (DEP) magnetophoresis (MP) to separate platelet cells, red white circulating tumor These were analyzed both independently simultaneously. To achieve optimal separation, Navier–Stokes equations, Newton's second law, system's electric magnetic fields modeled. Experiments conducted branched microchannel assess impact various parameters, including buffer rate, pinched section width, applied voltage, field frequency, intensity. revealed approximately 99% under conditions Wp=20 μm, Q2=1250 μl/h, f=100 kHz, V=3 V, M=1.5 T. findings demonstrate that while DEP MP individually enhance their simultaneous application significantly improves precision, achieving complete optimized system holds great promise applications analytical chemistry diagnostics, analysis.

Language: Английский

Citations

2
Label-Free and Rapid Microfluidic Design Rules for Circulating Tumor Cell Enrichment and Isolation: A Review and Simulation Analysis DOI Creative Commons
Muhammad Asraf Mansor, Chun Yang,

Kar Lok Chong

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(7), P. 6306 - 6322

Published: Feb. 11, 2025

Enriching and isolating circulating tumor cells (CTCs) have attracted significant interest due to their important role in early cancer diagnosis prognosis, allowing for minimally invasive approaches providing vital information about metastasis at the cellular level. This review comprehensively summarizes recent developments microfluidic devices CTC enrichment isolation. The advantages limitations of several are discussed, design specifications highlighted. We also developed a set methodologies rules label-free microfluidics such as spiral, deterministic lateral displacement (DLD) dielectrophoresis (DEP) allow researchers develop systematically effectively, promoting rapid research on design, fabrication, experimentation.

Language: Английский

Citations

1
Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances DOI Creative Commons
Hazal Kutluk, Martina Viefhues, Iordania Constantinou

et al.

Small Science, Journal Year: 2024, Volume and Issue: 4(4)

Published: Feb. 2, 2024

From deciphering infection and disease mechanisms to identifying novel biomarkers personalizing treatments, the characteristics of individual cells can provide significant insights into a variety biological processes facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited terms cost, contamination risks, sample volumes, times, throughput, sensitivity, selectivity. Although microfluidic approaches have been suggested as low‐cost, information‐rich, high‐throughput alternative conventional isolation methods, limitations such necessary off‐chip pre‐ post‐processing well systems designed for workflows restricted their applications. In this review, comprehensive overview recent advances integrated microfluidics on‐chip three prominent application domains provided: investigation somatic (particularly cancer immune cells), stem cells, microorganisms. Also, use cell separation (e.g., dielectrophoresis) unconventional or ways, which advance integration multiple systems, is discussed. Finally, critical discussion related current how they could be overcome provided.

Language: Английский

Citations

8
Tunable velocity-based deterministic lateral displacement for efficient separation of particles in various size ranges DOI Open Access
Ali Kheirkhah Barzoki, Rasool Dezhkam,

Amir Shamloo

et al.

Physics of Fluids, Journal Year: 2023, Volume and Issue: 35(7)

Published: July 1, 2023

Deterministic lateral displacement (DLD) is a promising method showing great potential in achieving high-resolution separation of suspended particles based on their size, through the use micropillars arranged periodic manner. In traditional approach to DLD, migration mode particle with specific size determined by critical diameter (Dc), which predetermined device's geometry. contrast conventional DLD chips that alter direction pillar array create an angle fluid streamlines, this paper proposes novel changing streamlines. The proposed enables fabrication tunable chip simple produce and can generate considerable Dc range adjusting two control parameters. first parameter ratio velocity between main outlet minor outlets, located at end microchannel outlets situated upper side. second flow rate sheath inlets controls particles' entrance position chamber. By manipulating these parameters, be easily adjusted. This features completely horizontal rows pillars provide values ranging from 1 25 μm.

Language: Английский

Citations

14
Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments DOI

Nahid Shanehband,

Seyed Morteza Naghib

Biochimie, Journal Year: 2024, Volume and Issue: 220, P. 122 - 143

Published: Jan. 3, 2024

Language: Английский

Citations

6
A novel microfluidic platform for MCF-7 separation: Arc-shaped deterministic lateral displacement microchannel DOI
Seyed Sepehr Mohseni, Ali Abouei Mehrizi,

Sogol Fathi

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113076 - 113076

Published: Feb. 1, 2025

Language: Английский

Citations

0
Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges DOI Creative Commons
Mark D. Tarn, Kirsty J. Shaw, Polly B. Foster

et al.

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing supercooled liquid water droplets, altering lifetime and radiative properties clouds having substantial impact on weather climate. However, INPs notoriously difficult model due lack information their global sources, sinks, concentrations, activity, necessitating development new instrumentation for quantifying characterizing in rapid automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups recent years as means performing droplet analysis INPs, enabling measurement hundreds or thousands droplets per experiment at temperatures down homogeneous water. The potential microfluidics extends far beyond this, with an entire toolbox bioanalytical separation detection techniques developed over 30 medical applications. Such methods could easily be adapted biological biogenic INP revolutionize field, example, identification quantification bacteria fungi. Combined miniaturized sampling techniques, we can envisage deployment microfluidic sample-to-answer platforms automated, user-friendly field that would enable greater understanding seasonal activity. Here, review various components such platform incorporate highlight feasibility, challenges, endeavor, from assays separations bioanalysis.

Language: Английский

Citations

0
Continuous and straightforward sorting of particles in microcavities with side outlets using inertial microfluidics DOI
Feng Shen, Jie Zhang, Chenchen Zhang

et al.

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)

Published: March 1, 2025

Inertial microfluidic technology has emerged as a highly promising approach for the separation of particles/cells, characterized by high throughput and label-free features. This study presents novel inertial chip design that enables continuous target particles at low Reynolds numbers (Re ≤ 36). To enhance its sorting performance, influences = 3.7–60), particle sizes (d 10 20 μm), outlet flow rates on efficiency purity are further examined, phase diagram optimal working conditions is obtained. The evolution field structure within comprehensively analyzed, which can be divided into three distinct regions, namely, main flow, sheath vortex. mechanism migration behavior across curved streamlines explored. device achieve maximum 94% large with fivefold increase in enrichment concentration, 31.3-fold purity, removal small reaching 97.1%. results demonstrate this facilitate direct larger based their size, presenting numerous advantages, such short microchannel length, number, minimal cell damage, ease operation. Hence, method represents an easy-to-use straightforward techniques anticipated to have practical application rare circulating tumor cells from complex solutions.

Language: Английский

Citations

0
Development of a Microfluidic System for Mitochondrial Extraction, Purification, and Analysis DOI

Rukhsar Ajmal,

Weisen Zhang,

Hui Liu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Mitochondria, as essential cellular organelles, play a key role in numerous diseases, from neurodegenerative disorders to cancer and rare conditions. The extraction of mitochondria cells has many applications disease diagnosis, pathological research, emerging mitochondrial transplantation therapy (MTT). Recent advancements microfluidic-on-chip systems offer promising improvements by enabling high-throughput processing, precise control, flexibility while facilitating integration with other devices platforms. Despite growing interest microfluidic (MME), there is lack comprehensive reviews on the latest developments this field. This review aims summarize recent well advantages limitations MME, providing deeper insights into microfluidic-based approaches for extraction, purification, analysis.

Language: Английский

Citations

0