Quantitation of Copper Tripeptide in Cosmetics via Fabric Phase Sorptive Extraction Combined with Zwitterionic Hydrophilic Interaction Liquid Chromatography and UV/Vis Detection DOI Creative Commons

Pantelitsa Pingou,

Anthi Parla, Abuzar Kabir

et al.

Separations, Journal Year: 2024, Volume and Issue: 11(10), P. 293 - 293

Published: Oct. 12, 2024

The increasing demand for effective cosmetics has driven the development of innovative analytical techniques to ensure product quality. This work presents and validation a zwitterionic hydrophilic interaction liquid chromatography method, coupled with ultraviolet detection, quantification copper tripeptide in cosmetics. A novel protocol sample preparation was developed using fabric phase sorptive extraction extract targeted analyte from complex cosmetic cream matrix, followed by chromatographic separation on ZIC®-pHILIC column. thorough investigation behavior HILIC column performed during method development. mobile consisted 133 mM ammonium formate (pH 9, adjusted hydroxide) acetonitrile at 40:60 (v/v) ratio, flow rate 0.2 mL/min. design experiments (DOE) approach allowed precise adjustments various factors influencing process, leading optimization analysis. demonstrated excellent linearity over concentration range 0.002 0.005% w/w tripeptide, correlation coefficient exceeding 0.998. limits detection quantitation were 5.3 × 10−4% 2.0 10−3% w/w, respectively. selectivity verified through successful other components within 10 min, establishing its suitability high-throughput quality control formulations.

Language: Английский

Impurity profiling of synthetic cyclic peptides based on orthogonality between hydrophilic-interaction and reversed-phase liquid chromatography DOI
Kenichi Yoshida, Satoshi Kato, Kanji Nagai

et al.

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: unknown, P. 465748 - 465748

Published: Jan. 1, 2025

Language: Английский

Citations

0
Focus on therapeutic peptides and their delivery DOI Creative Commons

Elise Rosson,

François Lux, Laurent David

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125555 - 125555

Published: April 1, 2025

Peptides are bioactive intermediates between small organic molecules and large biological compounds like antibodies or proteins. These play a unique valuable role as therapeutic agents, owing to their biochemical properties versatility in treating wide range of diseases such metabolic disorders, cancer therapy, antimicrobial anti-inflammatory agents. The global peptide therapeutics market is projected exceed USD 50 billion by 2024, reflecting the increasing demand interest this field. Therapeutic peptides offer an optimal balance specificity, safety, molecular size, providing greater precision targeting specific receptors with fewer off-target effects reduced toxicity compared small-organic drugs. also exhibit enhanced tissue penetration present simpler, cheaper manufacturing processes lower immunogenicity. To date, around 100 have attained clinical approval major markets, nearly half these approvals occurring past 20 years. This trend highlights growing importance potential modern medicine, explaining substantial associated treatments. review presents detailed comparison parenteral administration modes for peptides, specifically subcutaneous intravenous routes. We highlight how methods impact pharmacokinetic profiles influence patient outcomes, critical insights into advantages limitations each route. Finally, significant aspect its focus on innovative drug delivery systems formulations designed address challenges delivery, namely stability, bioavailability, efficacy.

Language: Английский

Citations

0
N‐ to C‐Peptide Synthesis, Arguably the Future for Sustainable Production DOI Creative Commons

Kinshuk Ghosh,

William D. Lubell

Journal of Peptide Science, Journal Year: 2025, Volume and Issue: 31(6)

Published: May 1, 2025

A revolution in peptide production arrived from the innovation of carboxylate to amine C- N-direction solid-phase synthesis. This cornerstone modern science has enabled multiple academic and industrial applications; however, process N-solid phase synthesis (C-N-SPPS) extreme mass intensity poor atom economy. Notably, C-N-SPPS relies upon use atom-intensive protecting groups, such as fluorenylmethyloxycarbonyl (Fmoc) protection wasteful excess protected amino acids coupling agents. On other hand, N- C-direction offers potential minimize may arguably enable more efficient means for manufacturing peptides. For example, amide bond formation been accomplished using methods employing thioesters, vinyl esters, transamidation achieve with minimal epimerization. review aims provide an overview C-peptide indicating advantages taking this avenue sustainable production.

Language: Английский

Citations

0
Potential Inhibitory Effect of the Peptide Melittin Purified from Apis mellifera Venom on CTX-M-Type Extended-Spectrum β-Lactamases of Escherichia coli DOI Creative Commons

Sheril Ramos-Alcántara,

María Alejandra Cornejo Napan,

Giovanni Lopez Campana

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(4), P. 403 - 403

Published: April 14, 2025

Background. Extended-spectrum β-lactamases (ESBLs) hydrolyze nearly all β-lactam antibiotics, affecting one of the most important groups antimicrobials used in Gram-negative infections. Among them, CTX-M is widespread type ESBL. This study aimed to evaluate hydrolytic activity CTX-M-type ESBLs following exposure antimicrobial peptide Melittin. Methods. Melittin was purified from Apis mellifera venom through ultrafiltration and characterized by SDS-PAGE. The minimum inhibitory concentration (MIC) against ESBL-producing E. coli determined broth microdilution method. inhibition ESBL’s sub-MIC doses quantified using a kinetic assay based on hydrolyzed nitrocefin. Additionally, effect expression blaCTX-M gene evaluated via RT-PCR. Results. fraction Apitoxin smaller than 10 kDa exhibited protein band corresponding Melittin, devoid higher molecular weight proteins. MIC ranged 50 80 µg/mL. Exposure at significantly inhibited ESBL activity, reducing it up 67%. However, transcription presence revealed no significant changes. Conclusions. able inhibit but not possibly indicating an translational or post-translational level.

Language: Английский

Citations

0
Screening of antioxidant peptides from soy protein isolate: In vitro activity validation and structure-activity relationships investigation through quantum chemical calculations DOI

Zhiyi Chen,

Yuting Xia, Guizhao Liang

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144616 - 144616

Published: May 1, 2025

Language: Английский

Citations

0
Advancements in superparamagnetic nanogels: A dual-role platform for diagnosis and targeted drug delivery DOI
Anil Pareek, Sanjesh Kumar, Devesh U. Kapoor

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125683 - 125683

Published: May 1, 2025

Language: Английский

Citations

0
Quantitation of Copper Tripeptide in Cosmetics via Fabric Phase Sorptive Extraction Combined with Zwitterionic Hydrophilic Interaction Liquid Chromatography and UV/Vis Detection DOI Creative Commons

Pantelitsa Pingou,

Anthi Parla, Abuzar Kabir

et al.

Separations, Journal Year: 2024, Volume and Issue: 11(10), P. 293 - 293

Published: Oct. 12, 2024

The increasing demand for effective cosmetics has driven the development of innovative analytical techniques to ensure product quality. This work presents and validation a zwitterionic hydrophilic interaction liquid chromatography method, coupled with ultraviolet detection, quantification copper tripeptide in cosmetics. A novel protocol sample preparation was developed using fabric phase sorptive extraction extract targeted analyte from complex cosmetic cream matrix, followed by chromatographic separation on ZIC®-pHILIC column. thorough investigation behavior HILIC column performed during method development. mobile consisted 133 mM ammonium formate (pH 9, adjusted hydroxide) acetonitrile at 40:60 (v/v) ratio, flow rate 0.2 mL/min. design experiments (DOE) approach allowed precise adjustments various factors influencing process, leading optimization analysis. demonstrated excellent linearity over concentration range 0.002 0.005% w/w tripeptide, correlation coefficient exceeding 0.998. limits detection quantitation were 5.3 × 10−4% 2.0 10−3% w/w, respectively. selectivity verified through successful other components within 10 min, establishing its suitability high-throughput quality control formulations.

Language: Английский

Citations

1