Putative Identification of 12 Isomeric and 21 Non-isomeric Compounds from Leaves of Rubus Alceifolius Poir Using Database-affinity UHPLC-Q-Exactive-Qrbitrap-MS/MS DOI Creative Commons
Xican Li,

Jingyuan Zeng,

Rongxin Ca

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract This is the first article to report of chemical profile leaves Rubus alceifolius Poir, a widely distributed medicinal plant in Eastern Hemisphere. Fresh R. were treated by set experimental protocols prepare lyophilized aqueous extract. A novel strategy was used analyze extract, i.e., The extract then analyzed using new strategy, database-affinity ultra-high-performance liquid chromatography-quadrupole-Exactive-Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS). Using MS/MS at full elucidation and comparison with database, 33 compounds putatively identified, including 12 flavonoid derivatives, 6 phenolic acid caffeoylquinic acids, two tea polyphenols, others. Especially, isomers have been strictly distinguished, apigenin vs 2'-hydroxydaidzein, luteolin 7-O-glucuronide scutellarin, (+) catechin (-) epicatechin, 3 -O- 4 5 acid, 3,4 dicaffeoylquinic 3,5 4,5- O -dicaffeoylquinic acid. In addition, 21 non-isomeric also found under both negative positive ion models, such as ellagic gallic Structural suggested that there biogenetic relationships among glycosidation hydroxylation. All these findings will help understand substance basis traditional functions Alceifolius . structural between These allow scientific community better functions.

Language: Английский

Comparative analysis of whole chloroplast genomes of three common species of Nekemias from vine tea DOI Creative Commons
Feng Yang, Shaoxiong Wu,

Zhengwen Yu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 17, 2024

Nekemias grossedentata (N. grossedentata) is a medicinal and edible plant. The young leaves tender stems are specifically utilized to manufacture vine tea, which traditionally employed in the treatment of conditions such as common cold fever, sore throat, jaundice hepatitis, other ailments. morphologically N. similar cantoniensis cantoniensis) megalophylla megalophylla), lead chaotic market supply. Numerious studies have confirmed that chloroplast genomes chromatography play important role plant classification. Here, whole (cp) three species were sequenced Illumina sequencing platform. Meanwhile, their chromatographic fingerprints constructed using high-performance liquid (HPLC). annotation results demonstrated typical quadripartite structures, with lengths 162,147 bp grossedentata), 161,981 162,500 cantoniensis), respectively. A total 89 /86 protein-coding genes, 37 tRNA gene 8 rRNA genes annotated. IR/SC boundary regions relatively conserved across species, although (rps19-rpl2, rpl32-trnL-UAG, ccsA-ndhD) exhibited nucleotide diversity values (Pi) variable sites higher than 1%. Phylogenetic analysis indicated had closer genetic relationship cantoniensis. Moreover, revealed main functional components relatedness highly morphological results. In conclusion, can be consider origin plants tea. This study provides appropriate information for identification, phylogeny, quality assessment genus will contribute standardization tea raw materials.

Language: Английский

Citations

0
Simultaneous Qualitative and Quantitative Analyses of 41 Constituents in Uvaria macrophylla Leaves Screen Antioxidant Quality-Markers Using Database-Affinity Ultra-High-Performance Liquid Chromatography with Quadrupole Orbitrap Tandem Mass Spectrometry DOI Creative Commons
Xiaoqiong Xu, Xican Li,

Shaoman Chen

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(20), P. 4886 - 4886

Published: Oct. 15, 2024

To date, no study has focused on

Language: Английский

Citations

0
Recent Advances in Analysis of Food and Beverages DOI Creative Commons
Javier Saurina

Separations, Journal Year: 2023, Volume and Issue: 10(3), P. 163 - 163

Published: Feb. 27, 2023

In this section, we summarize the characteristics of published papers according to different typologies [...]

Language: Английский

Citations

0
Library-based UHPLC-Q-Exactive-Orbitrap-MS Putative Identification of Isomeric and Non-isomeric Compounds from Shenling Baizhu Granule and its Pharmacopoeia Quality-marker Chemistry DOI Creative Commons
Xican Li,

Jingyuan Zeng,

Rongxin Cai

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: May 22, 2023

Shenling Baizhu Granule, a traditional Chinese medicine prescription comprising Renshen, Gancao, and Shanyao, has been widely consumed in China nowadays. However, its pharmacopoeia quality-marker (Q-marker) not defined The study thus used novel strategy, i.e., library-based ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry, to analyze lyophilized aqueous powder. strategy simultaneously putatively 77 compounds; Especially, 32 isomeric compounds have strictly distinguished, including ferulic acid isoferulic acid; liquiritigenin isoliquiritigenin; 3,4-dicaffeoylquinic 4,5-dicaffeoylquinic schaftoside isoschaftoside; ginsenoside Re Rd; Rc Rb2; daidzein 7,4’-dihydroxyflavone; luteolin kaempferol; butin S- naringenin; calycosin prunetin; isovitexin cosmosiin; orientin quercitrin; costunolide alantolactone; 2’-hydroxydaidzein, genistein, apigenin; Rg2, 20R -ginsenoside Rg3, 20S Rg3. In addition, 45 non-isomeric also identified. Combining with the quantum chemistry calculation UV-vis spectra scanning results, 4 are recommended as Q-marker candidates, glycyrrhizic acid, Re, Rb1, diosgenin. four can be qualitatively assess materials of prevent possible adulterant.

Language: Английский

Citations

0
Putative Identification of 12 Isomeric and 21 Non-isomeric Compounds from Leaves of Rubus Alceifolius Poir Using Database-affinity UHPLC-Q-Exactive-Qrbitrap-MS/MS DOI Creative Commons
Xican Li,

Jingyuan Zeng,

Rongxin Ca

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract This is the first article to report of chemical profile leaves Rubus alceifolius Poir, a widely distributed medicinal plant in Eastern Hemisphere. Fresh R. were treated by set experimental protocols prepare lyophilized aqueous extract. A novel strategy was used analyze extract, i.e., The extract then analyzed using new strategy, database-affinity ultra-high-performance liquid chromatography-quadrupole-Exactive-Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS). Using MS/MS at full elucidation and comparison with database, 33 compounds putatively identified, including 12 flavonoid derivatives, 6 phenolic acid caffeoylquinic acids, two tea polyphenols, others. Especially, isomers have been strictly distinguished, apigenin vs 2'-hydroxydaidzein, luteolin 7-O-glucuronide scutellarin, (+) catechin (-) epicatechin, 3 -O- 4 5 acid, 3,4 dicaffeoylquinic 3,5 4,5- O -dicaffeoylquinic acid. In addition, 21 non-isomeric also found under both negative positive ion models, such as ellagic gallic Structural suggested that there biogenetic relationships among glycosidation hydroxylation. All these findings will help understand substance basis traditional functions Alceifolius . structural between These allow scientific community better functions.

Language: Английский

Citations

0