Isolation, Identification, and Characterization of Phosphate-Solubilizing Bacteria from Tunisian Soils

Microorganisms, Journal Year: 2023, Volume and Issue: 11(3), P. 783 - 783

Published: March 18, 2023

Soil microorganisms play an important role in maintaining natural ecological balance through active participation carbon, nitrogen, sulfur, and phosphorous cycles. Phosphate-solubilizing bacteria (PSB) are of high importance the rhizosphere, enhancing solubilization inorganic phosphorus complexes into soluble forms available for plant nutrition. The investigation this species is major interest agriculture, as they can be used biofertilizers crops. In present study, 28 isolates PSB were obtained after phosphate enrichment soil samples from five Tunisian regions. Five identified by 16S rRNA gene sequencing including Pseudomonas fluorescens, P. putida, taiwanensis, Stenotrophomonas maltophilia, Pantoea agglomerans. Solid liquid Pikovskaya's (PVK) National Botanical Research Institute's (NBRIP) media containing insoluble tricalcium evaluation ability bacterial two methods: visual zone around colonies (halo) determination solubilized phosphates medium colorimetric method vanado-molybdate yellow. Based on results halo method, isolate each that showed higher index was selected method. media, ranging 535.70 to 618.57 µg mL-1 NBRIP medium, 374.20 544.28 PVK with highest values produced fluorescens. best reduction broth pH, which indicates organic acid production, achieved most PSB. Strong correlations observed between average capability solubilize both pH total content soil. production hormone indole acetic (IAA), promote growth, all species. Among them, fluorescens forest northern Tunisia IAA (50.4 ± 0.9 mL-1).

Language: Английский

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Ecological and sustainable implications of phosphorous-solubilizing microorganisms in soil

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(2)

Published: Jan. 26, 2024

Abstract Phosphorus (P) is a macronutrient that plants need to grow. However, most of the soil’s phosphorus still insoluble, making it difficult for absorb. This creates barrier ecologically responsible farming methods and calls innovative approaches solubilization. Solublizing microorganisms improve availability phosphorous in soil. The term “phosphorus-solubilizing microorganisms” (PSMs) describes various fungi or bacteria divide into more soluble forms. It shows how PSMs interact with their processes solubilize phosphorus. Soil pH, temperature, nutrient are only few parameters affecting its activity. investigated potential increase plant absorption use, thereby boosting agricultural yield usage efficiency. use effects on environment also evaluated. By using PSMs, farmers may less chemical fertilizers contribute runoff eutrophication waterways. Furthermore, soil structure, decrease losses, cycling, all which health long-term viability systems. Phosphorus-solubilizing have enormous promise environmentally land management. Better availability, greater output, pollution, better possible outcomes PSMs. further study required determine best application strategies, formulations, choices Incorporating operations can potentially environmental sustainability resilience. article will explore addressing critical challenges, including erosion, runoff, sustainable practices, resource conservation. Graphical

Language: Английский

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Phosphorus' future insecurity, the horror of depletion, and sustainability measures

International Journal of Environmental Science and Technology, Journal Year: 2024, Volume and Issue: 21(14), P. 9265 - 9280

Published: May 8, 2024

Language: Английский

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Use of Phosphorus-Solubilizing Microorganisms as a Biotechnological Alternative: A Review

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1591 - 1591

Published: Aug. 5, 2024

Microorganisms with the ability to dissolve phosphorus have potential release this essential nutrient into soil through natural solubilization processes, which allows for boosting plant growth and development. While literature reviews acknowledge their potential, unexplored territories concerning accessibility, application, effective integration sustainable agriculture necessitate further research. This manuscript employed distinct methodologies execute a bibliometric analysis review. The combined application of both enables holistic understanding domain landscape its innovative facets. For analysis, propositions Donthu Jia were utilized, supplemented by tools, such as Bibliometrix. review adhered systematic methodology predicated on Petersen's guidelines represent accurately, pinpointing trends gaps that could steer future, more detailed investigation uncovers an escalating interest in studying these microorganisms since 2000s, emphasizing significance context scarcity. It was also discerned India China, nations notable agricultural sectors high demand fertilizers, spearheaded research output subject. signifies substantial contribution progression scientific field. Furthermore, according consulted, phosphorus-solubilizing play pivotal role symbiotic interaction roots efficacious strategy counteract low availability sustainably enhance systems. Finally, contributes relevant examining existing empirical evidence special emphasis agriculture, improved mechanisms, recognition various microbial entities.

Language: Английский

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The effect of combined application of biochar and phosphate fertilizers on phosphorus transformation in saline-alkali soil and its microbiological mechanism

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175610 - 175610

Published: Aug. 18, 2024

Language: Английский

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The joint application of biochar and nitrogen enhances fruit yield, quality and water-nitrogen productivity of water-stressed greenhouse tomato under drip fertigation

Agricultural Water Management, Journal Year: 2023, Volume and Issue: 290, P. 108605 - 108605

Published: Nov. 23, 2023

The biochar application has been reported to have a positive effect on crop yield and nutrient utilization. However, the effects of joint nitrogen (N) soil physical properties, fruit yield, quality, water productivity (WP) partial factor (PFPN) water-stressed greenhouse tomato under drip fertigation remain poorly understood. In this study, two-season experiment was conducted in autumn 2021 spring 2022, involving two rates (0 30 t ha–1 only first season before sowing), N fertilization (175 250 kg ha–1), three irrigation levels (100%, 75%, 50% evapotranspiration). results showed that significantly decreased bulk density by 7–13%, but increased porosity 5.5%− 10% 43.2–73.8% compared with non-biochar treatment. affected total soluble solids (TSS), sugar (SS), vitamin C fruits. interaction PFPN WP 67.4% 76.3% 2021, 38.1% 51.8% respectively. Biochar rate level had significant TSS SS contents both seasons, seasons. application, yield. rate, were growing enhanced quality water-nitrogen tomato. Principal Component Analysis (PCA) revealed PC1 accounted for 47.7% 48%of variation while PC2 14.6% 15.6% This study underscores significance drip-fertigated cultivation highlights its sustained next season.

Language: Английский

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Rhizospheric Bacteria of Cover Legumes from Acidic Soils Are Capable of Solubilizing Different Inorganic Phosphates

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1101 - 1101

Published: May 29, 2024

Due to its adsorption with aluminum and iron hydroxides, phosphorus viability is low in acidic soils; thus, the aim of this study was isolate identify bacteria from rhizosphere four legumes growing soils Cumbaza Sub-basin, San Martín, Peru, as well characterize their ability solubilize phosphate phosphate. The isolation process conducted on TSA medium isolates were classified based origin morphocolonial characteristics, bacillary shape being most frequent, followed by cocci. To assess solubilization phosphates, liquid GELP employed. Sixteen strains selected, among which three stood out for effectiveness solubilizing AlPO4 (Sfcv-098-02, 22.65 mg L−1; Sfc-093-04, 26.50 Sfcv-041-01-2, 55.98 L−1) one FePO4 (Sfcr-043-02, 32.61 L−1). These molecularly characterized, identified Enterobacter sp., Pseudomonas Staphylococcus sp. Additionally, a decrease pH observed reactions, values ranging 5.23 3.29, enhanced solubilization. This suggests that selected could be used improve availability agricultural soils.

Language: Английский

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Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2493 - 2493

Published: Oct. 24, 2024

Tropical grassland soils, especially those with alkaline properties, often exhibit limited phosphorus availability due to its precipitation in insoluble forms. Phosphate-solubilizing bacteria (PSB) and rhizobia have demonstrated their potential enhance the of this nutrient promote growth forage legumes. This study, conducted under controlled conditions a mesh house, evaluated effect co-inoculation PSB, including Micrococcus sp. Sfcm-14-01, Agrobacterium Sfl-043-09, Enterobacter Sfcm-014-02 Sfcm-054-06, along (Ensifer terangae R1-012-02 Bradyrhizobium glycinis Rcm-025-01), different levels fertilization on legumes Leucaena leucocephala Centrosema macrocarpum. The results indicate significant increases various parameters, such as chlorophyll (SPAD), biomass (dry weight roots aerial parts) (mg), foliar concentration (ppm), available soil, particularly low-phosphorus conditions. highest level soil was achieved 75% recommended dose, resulting concentrations 13.73 ppm for L. 7.69 C. macrocarpum, representing 170.81% 240.27%, respectively, compared no or inoculation. These findings suggest that PSB native is promising strategy productivity mineral content tropical grazing systems, phosphorus-limited

Language: Английский

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Preparation of slow-release fertilizer derived from rice husk silica, hydroxypropyl methylcellulose, polyvinyl alcohol and paper composite coated urea.

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e42036 - e42036

Published: Jan. 1, 2025

There is a growing trend toward utilizing agricultural waste to create value-added products, addressing environmental concerns associated with their disposal. This study focuses on developing slow-release fertilizers (SRFs) using amorphous silica derived from rice husk, hydroxypropyl methylcellulose (HPMC), polyvinyl alcohol (PVA), paper, and urea. Experimental optimization was carried out the response surface methodology central composite design (RSM-CCD). The optimal formulation included 8.63 g of silica, 1.04 HPMC, 0.27 PVA. Two SRFs were prepared under these conditions: SRF1, consisting PVA, SRF2, which additionally incorporated coated paper. Characterization techniques such as Fourier Transform Infrared (FTIR) spectroscopy, X-ray diffraction (XRD) Scanning Electron microscopy (SEM) Brunauer-Emmett-Teller (BET) analysis used examine materials. rice-husk-derived exhibited pore size 2.140 nm BET area 690 m2/g, providing an excellent for nutrient encapsulation. Although addition paper minimally influenced behaviour however other components effectively reduced leakage by trapping nutrients. swelling analyzed in different media after 72 h, showing values 2.66, 2.54 (g/g) distilled water, 2.20, 2.58 pH 4, 1.86, 3.09 9 solutions. kinetics aligned Scott's second-order kinetic model. Urea release tests water revealed 94 % 97 at 24 h SRF1 respectively, compared 98 pure urea within 1 h. SRF2 demonstrated 48 followed first-order model both highlighting potential effective fertilizers.

Language: Английский

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Phosphate Solubilizing Microorganisms and Their Use in Sustainable Agriculture: A Review

Geomicrobiology Journal, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 29, 2025

Language: Английский

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