Identification of Cyanobacterial Estrogen-Like Compounds by Metabolomics and Machine Learning DOI

Jinmei Zi,

Justin R. Barker,

Yuanyan Zi

et al.

Published: Jan. 1, 2023

Cyanobacterial blooms, often dominated by Microcystis aeruginosa , are capable of producing estrogenic effects. It is important to identify specific compounds produced cyanobacteria, though this can prove challenging owing the complexity exudate mixtures. In study, we used untargeted metabolomics compare components exudates from two M. strains that differed with respect their ability produce microcystins, and across growth phases. We identified 416 chemicals found similar components, mainly organoheterocyclic (20.2%), organic acids derivatives (17.3%), phenylpropanoids polyketides (12.7%), benzenoids (12.0%), lipids lipid-like molecules (11.5%), oxygen (10.1%). then predicted group using random forest machine learning. Six (daidzin, biochanin A, phenylethylamine, rhein, o-Cresol, arbutin) belonging (3), (2), compound (1) were tested exhibited potency based upon E-screen assay. This study confirmed both contain properties, a growing concern in cyanobacteria management.

Language: Английский

Occurrence, Distribution and Toxins of Benthic Cyanobacteria in German Lakes DOI Creative Commons
Franziska Bauer,

Immanuel Wolfschlaeger,

Juergen Geist

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(8), P. 643 - 643

Published: July 25, 2023

Cyanobacteria are favored by climate change and global warming; however, to date, most research monitoring programs have focused on planktic cyanobacteria. Benthic cyanobacteria blooms also increase pose a risk animal human health; there is limited knowledge of their occurrence, distribution the toxins involved, especially in relation conspecifics. Therefore, we analyzed benthic life forms cyanobacterial communities 34 lakes Germany, including cyanotoxins. Community analyses were based microscopic examination Illumina sequencing 16S rRNA gene. The cyanotoxins carried out using LC-MS/MS ELISA. Observed mats containing consisted mainly Nostocales Oscillatoriales, being present 35% lakes. Anatoxin was abundant cyanotoxin samples, reaching maximum concentrations 45,000 µg/L, whereas microcystin predominate open-water up 18,000 µg/L. Based results, specific at toxic could be identified. Our findings suggest that should receive greater attention, ideally complementing existing sampling with little additional effort.

Language: Английский

Citations

13
Snapshot of cyanobacterial toxins in Pakistani freshwater bodies DOI
Uzma Batool, Nicolas Tromas, Dana F. Simon

et al.

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(16), P. 24648 - 24661

Published: March 6, 2024

Language: Английский

Citations

3
Harmful planktonic Microcystis and benthic Oscillatoria-induced toxicological effects on the Asian clam (Corbicula fluminea): A survey on histopathology, behavior, oxidative stress, apoptosis and inflammation DOI

Huiting Yang,

Xiaohong Gu,

Huihui Chen

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 283, P. 109961 - 109961

Published: June 16, 2024

Language: Английский

Citations

3
Assessment of estrogenic potential from exudates of microcystin-producing and non-microcystin-producing Microcystis by metabolomics, machine learning and E-screen assay DOI Creative Commons

Jinmei Zi,

Justin R. Barker,

Yuanyan Zi

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 470, P. 134170 - 134170

Published: April 9, 2024

Cyanobacterial blooms, often dominated by Microcystis aeruginosa, are capable of producing estrogenic effects. It is important to identify specific compounds produced cyanobacteria, though this can prove challenging owing the complexity exudate mixtures. In study, we used untargeted metabolomics compare components exudates from microcystin-producing and non-microcystin-producing M. aeruginosa strains that differed with respect their ability produce microcystins, across two growth phases. We identified 416 chemicals found similar components, mainly organoheterocyclic (20.2%), organic acids derivatives (17.3%), phenylpropanoids polyketides (12.7%), benzenoids (12.0%), lipids lipid-like molecules (11.5%), oxygen (10.1%). then predicted group using random forest machine learning. Six (daidzin, biochanin A, phenylethylamine, rhein, o-Cresol, arbutin) belonging (3), (2), compound (1) were tested exhibited potency based upon E-screen assay. This study confirmed both contain properties, a growing concern in cyanobacteria management.

Language: Английский

Citations

2
High altitude may limit production of secondary metabolites by cyanobacteria DOI
Iwona Jasser, Nataliia Khomutovska, Małgorzata Sandzewicz

et al.

Ecohydrology & Hydrobiology, Journal Year: 2024, Volume and Issue: 24(2), P. 271 - 280

Published: April 1, 2024

Language: Английский

Citations

2
Cyanotoxins in Epipelic and Epiphytic Cyanobacteria from a Hypersaline Coastal Lagoon, an Environmental Hazard in Climate Warming Times and a Potential Source of New Compounds DOI Creative Commons

Yerai Gómez-Leyva,

Alejandro Torrecillas, Marina Aboal

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(8), P. 334 - 334

Published: July 24, 2024

Cyanobacterial biodiversity and potential toxicity in coastal lagoons have barely been studied despite these transitional water systems being very important conservation for the preservation of economic resources. Most affected by eutrophication, climate change will severely affect them promoting cyanobacteria growth, especially Mediterranean areas. This study aims to characterize diversity epipelic epiphytic species a lagoon their toxins production (microcystins saxitoxins). Strains were isolated genetically identified. Toxins extracted quantified LC/MS-MS. All taxa belong former Oscillatoriales. The presence Nodosilinea Toxifilum is reported first time Spanish waters, but Pseudanabaena, Phormidium, Geitlerinema Synechococcus also formed part benthic mats. strains contained Microcystin-YR (MC-YR), saxitoxin (STX) was present only extracts Pseudanabena. MC-LY, MC-LW [D-Asp3] MC-LR detected MC-LF Toxifilum, at concentrations that did not permit quantification. may represent hazard, an opportunity obtain potentially interesting compounds should be further studied.

Language: Английский

Citations

1
Floridanema gen. nov. (Aerosakkonemataceae, Aerosakkonematales ord. nov., Cyanobacteria) from benthic tropical and subtropical fresh waters, with the description of four new species DOI
Jéssica Aparecida Silva Moretto, David E. Berthold, Forrest W. Lefler

et al.

Journal of Phycology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Cyanobacteria exhibit a vast diversity from polar to tropical environments. Though much work has been done on elucidating their biodiversity, knowledge the occurrence, and toxicity of benthic cyanobacteria is limited when compared planktonic forms. Integrating molecular techniques with ecological morphological analyses become essential in untangling cyanobacterial diversity, particularly for taxa such as cryptic “ Lyngbya .” Molecular markers 16S rRNA gene whole genome sequencing have significantly improved taxonomy cyanobacteria. Building these advancements, this study characterizes isolates various locations Florida, USA, Orange Walk, Belize, resulting identification novel genus, Floridanema , four new species ( F. aerugineum evergladense flaviceps fluviatile ). This genus commonly occurs canals, ponds, lakes rivers. By integrating ecological, morphological, genomic analyses, provides support family Aerosakkonemataceae establishment order Aerosakkonematales. The LC–MS data revealed that strains do not produce microcystins, nodularin‐R, or anabaenopeptins.

Language: Английский

Citations

1
Cyanobacteria and Macroinvertebrate Relationships in Freshwater Benthic Communities beyond Cytotoxicity DOI Creative Commons
Nicolás Ubero‐Pascal, Marina Aboal

Toxins, Journal Year: 2024, Volume and Issue: 16(4), P. 190 - 190

Published: April 15, 2024

Cyanobacteria are harmful algae that monitored worldwide to prevent the effects of toxins they can produce. Most research efforts have focused on direct or indirect human populations, with a view gain easy accurate detection and quantification methods, mainly in planktic communities, but increasing interest shown benthos. However, cyanobacteria played fundamental role from very beginning both development our planet’s biodiversity construction new habitats. These organisms colonized almost every possible benthic environment earth, including most extreme ones, display vast number adaptations. All this explains why important only phototrophs some The negative cyanotoxins macroinvertebrates been demonstrated, usually under conditions far natural, forms exposure, toxin concentration, composition. cohabitation invertebrate groups is long-standing has probably contributed detoxification means, which would explain survival species inside colonies. This review focuses cyanobacteria, their capacity produce several types toxins, relationships beyond toxicity.

Language: Английский

Citations

0
Benthic cyanobacterial proliferations drive anatoxin production throughout the Klamath River watershed, California, USA DOI

Laurel Genzoli,

Robert O. Hall,

Timothy G. Otten

et al.

Freshwater Science, Journal Year: 2024, Volume and Issue: 43(3), P. 307 - 324

Published: June 24, 2024

Language: Английский

Citations

0
Fluorescence capturing behaviour of cyanobacterial resilience: Insights into UV‐exposed ecosystems and its environmental applications DOI

R. Prathima,

Kanthesh M. Basalingappa,

Sai Kavya D

et al.

Luminescence, Journal Year: 2024, Volume and Issue: 39(9)

Published: Sept. 1, 2024

Abstract Cyanobacteria are resilient microorganisms and thrive in environments exposed to UV radiation, ranging from ocean surfaces scorching hot springs dry expanses. ‘Cyanobacterial Resilience’ refers their ability withstand revealing intricate genomic secrets adaptive mechanisms ensuring survival. These include metabolic adaptations, robust DNA repair systems UV‐protective compounds such as Scytonemin Mycosporine, vital for shielding against radiation crucial pioneers UV‐exposed ecosystems, highlighting resilience adaptability. Some cyanobacteria exhibit luminescence, emitting blue‐green light due phycobiliproteins, while bioluminescence cyanobacteria, if it occurs, involves different rather than luciferins luciferase enzymes. This luminescence holds promise various biotechnological applications, biosensors, imaging probes carbon sequestration, participating photocatalytic processes water purification CO 2 conversion, contributes solar simulation studies advance photosynthesis renewable energy technologies. The versatile applications of these materials highlight ecological importance potential addressing global challenges. In conclusion, highlights the remarkable adaptation strategies environments. It emphasises role innovators biological technological domains, providing insights into enduring impact on ecosystems scientific advancement.

Language: Английский

Citations

0