Polystyrene microplastics enhance microcystin-LR-induced cardiovascular toxicity and oxidative stress in zebrafish embryos

Environmental Pollution, Journal Year: 2024, Volume and Issue: 352, P. 124022 - 124022

Published: April 26, 2024

Language: Английский

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Transgenerational effects of extracts containing Microcystin-LR exposure on reproductive toxicity and offspring growth inhibition in a model organism zebrafish

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 268, P. 106860 - 106860

Published: Feb. 5, 2024

Language: Английский

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Impact of algicidal fungus Aspergillus welwitschiae GF6 on harmful bloom-forming cyanobacterium Microcystis aeruginosa: Growth and physiological responses

Chemosphere, Journal Year: 2025, Volume and Issue: 372, P. 144090 - 144090

Published: Jan. 13, 2025

Language: Английский

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Spatial multi-omics analysis of metabolic heterogeneity in zebrafish exposed to microcystin-LR and its disinfection byproducts

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123599 - 123599

Published: April 1, 2025

Language: Английский

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A portable EIS-based biosensor for the detection of microcystin-LR residues in environmental water bodies and simulated body fluids

The Analyst, Journal Year: 2024, Volume and Issue: 149(7), P. 2170 - 2179

Published: Jan. 1, 2024

Due to the eutrophication of water bodies around world, there is a drastic increase in harmful cyanobacterial blooms leading contamination with cyanotoxins. Chronic exposure cyanotoxins such as microcystin leads oxidative stress, inflammation, and liver damage, potentially cancer. We developed novel easy-to-use electrochemical impedance spectroscopy-based immunosensor by fabricating stencil-printed conductive carbon-based interdigitated microelectrodes immobilising them cysteamine-capped gold nanoparticles embedded polyaniline. It has been also coupled custom handheld device enabling regular on-site assessment, especially resource-constrained situations encountered developing countries. The sensor able detect microcystin-LR up 0.1 μg L

Language: Английский

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Algae blooms with resistance in fresh water: Potential interplay between Microcystis and antibiotic resistance genes

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 940, P. 173528 - 173528

Published: May 25, 2024

Language: Английский

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Subchronic Microcystin-LR Aggravates Colorectal Inflammatory Response and Barrier Disruption via Raf/ERK Signaling Pathway in Obese Mice

Toxins, Journal Year: 2023, Volume and Issue: 15(4), P. 262 - 262

Published: April 1, 2023

Microcystin-LR (MC-LR) is an extremely poisonous cyanotoxin that poses a threat to ecosystems and human health. MC-LR has been reported as enterotoxin. The objective of this study was determine the effect mechanism subchronic toxicity on preexisting diet-induced colorectal damage. C57BL/6J mice were given either regular diet or high-fat (HFD) for 8 weeks. After weeks feeding, animals supplied with vehicle 120 μg/L via drinking water another weeks, their stained H&E detect microstructural alterations. Compared CT group, HFD + HFD-treatment group induced significant weight gain in mice. Histopathological findings showed HFD- groups caused epithelial barrier disruption infiltration inflammatory cells. raised levels inflammation mediator factors decreased expression tight junction-related compared group. p-Raf/Raf p-ERK/ERK significantly increased Additionally, treated HFD, injury further aggravated These suggest by stimulating Raf/ERK signaling pathway, may cause disruption. This suggests treatment exacerbate HFD. offer unique insights into consequences harmful mechanisms provide strategies preventing treating intestinal disorders.

Language: Английский

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Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis

Toxins, Journal Year: 2023, Volume and Issue: 15(7), P. 411 - 411

Published: June 25, 2023

Freshwater prokaryotic cyanobacteria within harmful algal blooms produce cyanotoxins which are considered major pollutants in the aquatic system. Direct exposure to through inhalation, skin contact, or ingestion of contaminated drinking water can target liver and may cause hepatotoxicity. In current study, we investigated effect low concentrations on cytotoxicity, inflammation, modulation unfolded protein response (UPR), steatosis, fibrosis signaling human hepatocytes cell models. Exposure microcystin-LR (MC-LR), microcystin-RR (MC-RR), nodularin (NOD), cylindrospermopsin (CYN) bipotent progenitor line HepaRG hepatocellular carcinoma (HCC) lines HepG2 SK-Hep1 resulted increased toxicity. MC-LR, NOD, CYN differentially regulated inflammatory signaling, activated UPR lipogenic gene expression, induced cellular steatosis fibrotic HCC cells. also AKT/mTOR inhibited autophagy. Chronic upregulated expression biomarkers. Moreover, RNA sequencing (RNA seq) data suggested that hepatocytes, HepaRG, cells MC-LR modulated levels several genes regulate non-alcoholic fatty disease (NAFLD). Our suggest hepatotoxicity promote NAFLD progression.

Language: Английский

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Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS

Chemosensors, Journal Year: 2023, Volume and Issue: 11(10), P. 511 - 511

Published: Sept. 25, 2023

Dairy milk holds a prominent position as widely consumed food, particularly among infants and children. However, it is crucial to address the presence of multiple natural toxic compounds that may co-occur in dairy ensure its safety prior consumption. Aflatoxin M1 (AFM1), an emerging mycotoxin interest, potential contaminant animals who ingest aflatoxin B1 (AFB1). The toxin regulated European Union under Commission Regulation No 1881/2006. Unfortunately, there notable lack data concerning transfer various microbial contaminants into and, therefore, their occurrences. In this study, simple sensitive LC-MS/MS method was developed validated for quantification cyanotoxins (microcystin congeners nodularin) AFM1 by main analytical guidelines. Toxins are extracted with methanol 80%, followed SPE clean-up step before analysis. LOQ fixed at 1 µg/L nine 0.05 AFM1. Recoveries were measured between 82.67% 102%. To best our knowledge, no other methods available simultaneous mycotoxins milk.

Language: Английский

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Chronic exposure to low-dose MC-LR induces ileal inflammation in mice through the PI3K/AKT/mTOR pathway

Journal of Toxicology and Environmental Health, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 9

Published: Jan. 10, 2025

The global phenomenon of cyanobacterial bloom pollution is spreading globally due to climate change and eutrophication. It well established that harmful cyanobacteria produce a wide range toxins including microcystin-LR (MC-LR), cyclic heptapeptide toxin known damage various organs. intestinal tract the main site MC-LR absorption one targets susceptible toxicity. Currently, studies on enterotoxic effects predominantly focused colorectum, with limited investigations addressing impact microcystins small intestine. Therefore, aim our study was examine chronic 9-month exposure mice low-dose 120 μg/L in drinking water ileal inflammation potential mechanisms underlying these effects. Our findings showed chronically administered disorganized epithelial cells, lymphocytic infiltration disturbed crypt arrangement were detected. results qPCR Western blot demonstrated that, comparison control, mRNA expression levels pro-inflammatory factors IL-6, IL-17, IL-18, IFN-γ markedly elevated tissue treated MC-LR, associated significant increases protein p-PI3K, p-AKT, p-mTOR. Taken together, evidence indicates induces histopathological involved activation PI3K/AKT/mTOR signaling pathway.

Language: Английский

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