Current Research in Biotechnology,
Journal Year:
2023,
Volume and Issue:
6, P. 100153 - 100153
Published: Jan. 1, 2023
Aflatoxins
(AFs)
are
naturally
occurring
mycotoxins
known
to
cause
a
considerable
threat
food
safety
and
affect
animal
health.
Rapid
reliable
analytical
methods
crucial
for
preventing
AF
contamination
in
the
global
supply
chain.
Many
conventional
detection
involve
complex
sample
preparation
steps,
lengthy
analysis
times,
multiple
handling
stages
which
lead
delays
obtaining
results,
addition
being
unaffordable
many
settings
with
resource
constraints.
Herein,
we
demonstrate
proof
of
concept
competitive
immunochromatographic
(IC)
strip
test
quantification
total
using
commercially
available
antibodies
low-cost
portable
CubeTM
analyzer.
We
conducted
preliminary
testing
our
point-of-need
(PON)
method
corn
samples
results
indicated
good
agreement
when
compared
gold
standard
HPLC
method.
Furthermore,
range
5–50
ppb
time
5
min,
makes
this
technology
suitable
rapid
meets
regulatory
requirements
samples.
also
real-time
data
sharing
capabilities
reader
proof-of-concept
centralized
cloud-based
databank,
that
developed
provide
timely
monitoring
different
parts
systems.
It
is
critical
be
easily
accessible
within
systems
dashboard
enable
early
warning,
data-driven
decision-making,
interventions,
improve
overall
coordination
between
various
stakeholders
system.
Comprehensive Reviews in Food Science and Food Safety,
Journal Year:
2024,
Volume and Issue:
23(3)
Published: April 17, 2024
Abstract
Mycotoxins,
ubiquitous
contaminants
in
food,
present
a
global
threat
to
human
health
and
well‐being.
Mitigation
efforts,
such
as
the
implementation
of
sound
agricultural
practices,
thorough
food
processing,
advancement
mycotoxin
control
technologies,
have
been
instrumental
reducing
exposure
associated
toxicity.
To
comprehensively
assess
mycotoxins
their
toxicodynamic
implications,
deployment
effective
predictive
strategies
is
imperative.
Understanding
manner
action,
transformation,
cumulative
toxic
effects
mycotoxins,
moreover,
interactions
with
matrices
can
be
gleaned
through
gene
expression
transcriptome
analyses
at
cellular
molecular
levels.
MicroRNAs
(miRNAs)
govern
target
genes
enzymes
that
play
pivotal
roles
physiological,
pathological,
toxicological
responses,
whereas
acute
phase
proteins
(APPs)
exert
regulatory
over
metabolism
therapeutic
agents,
both
endogenously
posttranscriptionally.
Consequently,
this
review
aims
consolidate
current
knowledge
concerning
role
miRNAs
initiation
pathways
by
explores
potential
APPs
biomarkers
following
exposure.
The
findings
research
highlight
utility
indicators
for
detection
management
biological
processes.
These
markers
offer
promising
avenues
enhancing
safety
quality
products.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 21, 2025
Abstract
Aflatoxin
B
1
(AFB
)
is
a
highly
potent
mycotoxin
that
poses
serious
threat
to
human
and
animal
health.
This
study
investigated
the
biodegradation
of
AFB
by
supernatant
submerged
cultured
Trametes
hirsuta
,
with
focus
on
identifying
characterizing
responsible
enzyme(s).
The
extracellular
enzymes
white-rot
mushroom
were
extracted
from
pre-separated
using
anion
exchange
fast
protein
liquid
chromatography
(FPLC).
To
pinpoint
specific
enzyme,
eluted
fractions
exhibiting
highest
degradation
activity
subjected
detailed
biochemical
proteomic
analyses.
A
second
purification
step,
ultrafiltration,
yielded
an
electrophoretically
pure
enzyme.
Sequencing
tryptic
peptides
nano-LC
system
coupled
qQTOF
mass
spectrometer
identified
enzyme
as
lignolytic
phenoloxidase.
exhibited
molecular
55.6
kDa
achieved
impressive
rate
77.9%
under
optimized
experimental
conditions.
first
fungal
phenoloxidase
capable
aflatoxin
without
requiring
hydrogen
peroxide
cofactor,
highlighting
its
unique
catalytic
mechanism.
It
may
be
used
in
remediation
strategies,
such
treating
surfaces
contaminated
fruits,
vegetables,
nuts.
Life,
Journal Year:
2025,
Volume and Issue:
15(2), P. 140 - 140
Published: Jan. 21, 2025
Fungal
contamination
represents
a
significant
threat
during
peanut
storage.
In
this
research,
strain
of
Penicillium
spp.
was
used
as
test
microorganism
to
assess
its
viability
storage
over
30
days
at
three
different
temperatures
(4,
15,
and
25
°C)
two
inoculum
levels
(low-2
log
CFU/g
high-5
CFU/g).
Two
types
were
tested:
the
Spanish
type
Virginia
type.
Independently
spore
age,
fungus
survived
throughout
period,
in
some
samples
(low
Virginia-type
peanuts)
level
increased.
second
phase,
four
drying
treatments,
differing
temperature
duration,
tested.
inactivation
primarily
depended
on
temperature,
while
duration
process
did
not
have
effect.
At
low
temperatures,
fungal
minimal
statistically
significant,
suggesting
that
low-temperature
treatments
could
pose
potential
health
risk.
